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The map label for this gene is vipB [H]

Identifier: 187734545

GI number: 187734545

Start: 37306

End: 38232

Strand: Reverse

Name: vipB [H]

Synonym: Amuc_0029

Alternate gene names: 187734545

Gene position: 38232-37306 (Counterclockwise)

Preceding gene: 187734546

Following gene: 187734544

Centisome position: 1.44

GC content: 57.07

Gene sequence:

>927_bases
ATGAAGATTCTCGTAACCGGCGGCGCCGGATTCATCGGTTCCCACATTGTGGAACACTATCAGGATAAGGCGGAGGAAAT
CCGTGTGCTGGACAACCTGCGCACGGGCTATCTCAAGAACCTGGAAGGGCTCAGGCACACGTTCATCGAAGGTTCCATCT
GCGACCGGGAGCTGGTGCGCCAGGCGGTGCAGGGAGTGGACTATATTTTCCACATGGCCGCGCTCGTCTCCGTGCCGGAA
TCCATGAGCAAGATCAGCGAATGCATCGACATCAACGTCAACGGTTTGCTGAACGTGCTGGAGGAAGCTTCCGCCGCCGG
AGTCAAAAAAATCGTGCTGGCGTCTTCCGCCGCCATTTACGGAGACAATCCCACGGTGCCCAAACTGGAAACCATGTACC
CGGAACCCAAGAGTCCCTATGCCATTACCAAGCTGGATGGGGAATACTACCTCAACATGTTCCGGGCGGAAGGAAAAATT
AATACGGCAGCCGTGCGCTTCTTCAATGTCTTCGGCCCCCGGCAGGACCCCAAGGGCGCCTATGCCGCAGCCGTGCCCAT
TTTCATTGAAAAAGCTGTCAAAGGAGAAGACATCACCGTGTATGGGGACGGCTCCCAGACGCGCGATTTCATTTATGTGA
AAGACATTGTAGGAGCCCTCACCTTTGTGGCGGAACACCCGGAAGTCACCGGCGTGTTCAATGCCGGTTACGGCGGCCAG
ATCACCATTGAAGAGCTGGCGCAGAACATCATCAAGGCTGCCGGGTCTTCCTCCAAGGTGCTTCATGCCCCGGAACGTCC
GGGAGACGTCAAGCATTCCCGCGCCTGTGCGGACAAGCTCCGCAATGCCGGATGGCAGCCCAGGCATACTTTGCCGGAAG
GCCTGGCGACGACGCTGGAATACTTCAAGGGCATTCTGGGCAGGTAA

Upstream 100 bases:

>100_bases
GCACGCAGATGGCCCTGGTAGAATCCATTGAGGCGCAGATGGATGAAATGAAGGCCATTGTGGACCGCTATTAACCAATA
TCTACCAACATCGGAAAAAC

Downstream 100 bases:

>100_bases
CCTTCTGTAATTTCCGGTTTTATATTACCCCCGGACTGCCTCCGCTCCTGCAGCGGAGGCAGTCTTTTTTATGGGAAACT
GGGGCTGTGTATGACAAATT

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKILVTGGAGFIGSHIVEHYQDKAEEIRVLDNLRTGYLKNLEGLRHTFIEGSICDRELVRQAVQGVDYIFHMAALVSVPE
SMSKISECIDINVNGLLNVLEEASAAGVKKIVLASSAAIYGDNPTVPKLETMYPEPKSPYAITKLDGEYYLNMFRAEGKI
NTAAVRFFNVFGPRQDPKGAYAAAVPIFIEKAVKGEDITVYGDGSQTRDFIYVKDIVGALTFVAEHPEVTGVFNAGYGGQ
ITIEELAQNIIKAAGSSSKVLHAPERPGDVKHSRACADKLRNAGWQPRHTLPEGLATTLEYFKGILGR

Sequences:

>Translated_308_residues
MKILVTGGAGFIGSHIVEHYQDKAEEIRVLDNLRTGYLKNLEGLRHTFIEGSICDRELVRQAVQGVDYIFHMAALVSVPE
SMSKISECIDINVNGLLNVLEEASAAGVKKIVLASSAAIYGDNPTVPKLETMYPEPKSPYAITKLDGEYYLNMFRAEGKI
NTAAVRFFNVFGPRQDPKGAYAAAVPIFIEKAVKGEDITVYGDGSQTRDFIYVKDIVGALTFVAEHPEVTGVFNAGYGGQ
ITIEELAQNIIKAAGSSSKVLHAPERPGDVKHSRACADKLRNAGWQPRHTLPEGLATTLEYFKGILGR
>Mature_308_residues
MKILVTGGAGFIGSHIVEHYQDKAEEIRVLDNLRTGYLKNLEGLRHTFIEGSICDRELVRQAVQGVDYIFHMAALVSVPE
SMSKISECIDINVNGLLNVLEEASAAGVKKIVLASSAAIYGDNPTVPKLETMYPEPKSPYAITKLDGEYYLNMFRAEGKI
NTAAVRFFNVFGPRQDPKGAYAAAVPIFIEKAVKGEDITVYGDGSQTRDFIYVKDIVGALTFVAEHPEVTGVFNAGYGGQ
ITIEELAQNIIKAAGSSSKVLHAPERPGDVKHSRACADKLRNAGWQPRHTLPEGLATTLEYFKGILGR

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=309, Percent_Identity=32.6860841423948, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI7657641, Length=320, Percent_Identity=29.6875, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI56237023, Length=349, Percent_Identity=27.2206303724928, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI56118217, Length=349, Percent_Identity=27.2206303724928, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI189083684, Length=349, Percent_Identity=27.2206303724928, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI193211614, Length=229, Percent_Identity=31.0043668122271, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI8393516, Length=229, Percent_Identity=31.0043668122271, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786974, Length=330, Percent_Identity=32.1212121212121, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788353, Length=339, Percent_Identity=28.023598820059, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI48994969, Length=335, Percent_Identity=28.6567164179104, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1790049, Length=314, Percent_Identity=29.2993630573248, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788365, Length=325, Percent_Identity=24.3076923076923, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=32.2884012539185, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17568069, Length=324, Percent_Identity=29.6296296296296, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532424, Length=322, Percent_Identity=25.4658385093168, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=338, Percent_Identity=27.5147928994083, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=340, Percent_Identity=27.3529411764706, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319493, Length=319, Percent_Identity=29.7805642633229, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356223, Length=315, Percent_Identity=32.3809523809524, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19923002, Length=346, Percent_Identity=28.6127167630058, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 33589; Mature: 33589

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSHIVEHYQDKAEEIRVLDNLRTGYLKNLEGLRHTFIEGSICDRELVR
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QAVQGVDYIFHMAALVSVPESMSKISECIDINVNGLLNVLEEASAAGVKKIVLASSAAIY
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GDNPTVPKLETMYPEPKSPYAITKLDGEYYLNMFRAEGKINTAAVRFFNVFGPRQDPKGA
CCCCCCCCHHCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
YAAAVPIFIEKAVKGEDITVYGDGSQTRDFIYVKDIVGALTFVAEHPEVTGVFNAGYGGQ
HHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECCCCCE
ITIEELAQNIIKAAGSSSKVLHAPERPGDVKHSRACADKLRNAGWQPRHTLPEGLATTLE
EEHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
YFKGILGR
HHHHHHCC
>Mature Secondary Structure
MKILVTGGAGFIGSHIVEHYQDKAEEIRVLDNLRTGYLKNLEGLRHTFIEGSICDRELVR
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QAVQGVDYIFHMAALVSVPESMSKISECIDINVNGLLNVLEEASAAGVKKIVLASSAAIY
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GDNPTVPKLETMYPEPKSPYAITKLDGEYYLNMFRAEGKINTAAVRFFNVFGPRQDPKGA
CCCCCCCCHHCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
YAAAVPIFIEKAVKGEDITVYGDGSQTRDFIYVKDIVGALTFVAEHPEVTGVFNAGYGGQ
HHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECCCCCE
ITIEELAQNIIKAAGSSSKVLHAPERPGDVKHSRACADKLRNAGWQPRHTLPEGLATTLE
EEHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
YFKGILGR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]