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The map label for this gene is ada [H]

Identifier: 186477832

GI number: 186477832

Start: 3467091

End: 3468221

Strand: Reverse

Name: ada [H]

Synonym: Bphy_3088

Alternate gene names: 186477832

Gene position: 3468221-3467091 (Counterclockwise)

Preceding gene: 186477834

Following gene: 186477831

Centisome position: 99.68

GC content: 66.49

Gene sequence:

>1131_bases
ATGAACACAGCCTTCTCTCACGCCGTGCCCGCCACGTTCCATAACGAAGACGAGCGCTGGGCGGCTGTGCTGAAACGCGA
CATGTCCGCCGACGGCGCGTTCTTTTACGGCGTCCGCACGACGGGCGTGTTCTGCCGTCCGTCGTGCGCGTCGCGACTAC
CGCGTCGTGAGAATGTCGACTTCTTCGAATCGGCCGACGACGCGCGCGCTGCGGGCTATCGCGAATGCAAGCGCTGCCAG
CCGGGCGGCCTGCCGCGCGAACTCGAGATCGTCAAGCGCGCGTGCGCAACGCTCGACGCCTACCCGCAGCAGCGCTTGAC
GCTCGCGCAGTTGAGCGATGCCGTGCACATCAGCCCATTTCATCTGCAGCGGCTGTTCAAACGCGTGGTTGGCGTCTCGC
CGCGGCAATATCAGGCTGCGCAACGCGGCGCGGCGTTTCGCGACGCGCTTCAACGGGGCGCGGACGTCACACGTGCGACG
GTCGACGCTGGCTTCGGCTCGCCGTCTCGCATGTACGACACGGCGGCCGCCGAACTGGGCATGGCGCCGTCCGAATATCG
ACGCAAAGGCGCCGGATTGCGCGTCCGTTACGCGAGTGCGCTGACGTCGCTTGGCTTCGTGCTGGTGGCGACGACGGACA
AGGGCATTTGCAAGATCGCATTCGGCGATGACGAAGCGCCGCTTATCGACGATCTGCGCCAAGAATTCGCAAGCGCGGAA
CTGCTTGGCGATCCTGTGCGGCTCGCACCGTTCATCGAGCAGATCGACGCGTACCTGCAAGGCAAGCGCGAGCGCTTCGA
TCTGCCGCTCGACATCGCCGCCACCGCCTTCCAGCAGCGCGTGTGGGAAGCGCTGCGCCGCATCCCTTACGGGGAGACAC
GCACCTATTCCCAGATTGCCGAAGCGGTCGGATTGCCGCGTGCGGTGCGCGCCGTCGCCAACGCGTGCGCATCCAACCCG
GTGGCGCTTGCGATACCCTGTCATCGCGTCGTGCAGAAGGGCGGCGCACTCGCGGGTTATCGCTGGGGCACCGCGCGCAA
GGCGACGCTGCTCGACACGGAAGCGCAGCGTGAATCCAGCACTGCCGACGAAGCCACCCCCACGACCGCCGACATGGACC
ACGCCGCTTGA

Upstream 100 bases:

>100_bases
CCGTCGAAACGCCCCCAATGACACCGCAAGAATCGGAGCGTACCGTGTAAGGGCGCTCGCTACAGTGGTAAGCATCAAGA
CAGTTCCCTGGGGCAAAACG

Downstream 100 bases:

>100_bases
GCACCACGCCGACAGCCGTACTCGAACTGCCGTTCAAAGCGCCCTATGACTGGCCGCGCATCCTGCGATTTTTCGGCGGA
CGCGCGACGCCGGGCGTCGA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNTAFSHAVPATFHNEDERWAAVLKRDMSADGAFFYGVRTTGVFCRPSCASRLPRRENVDFFESADDARAAGYRECKRCQ
PGGLPRELEIVKRACATLDAYPQQRLTLAQLSDAVHISPFHLQRLFKRVVGVSPRQYQAAQRGAAFRDALQRGADVTRAT
VDAGFGSPSRMYDTAAAELGMAPSEYRRKGAGLRVRYASALTSLGFVLVATTDKGICKIAFGDDEAPLIDDLRQEFASAE
LLGDPVRLAPFIEQIDAYLQGKRERFDLPLDIAATAFQQRVWEALRRIPYGETRTYSQIAEAVGLPRAVRAVANACASNP
VALAIPCHRVVQKGGALAGYRWGTARKATLLDTEAQRESSTADEATPTTADMDHAA

Sequences:

>Translated_376_residues
MNTAFSHAVPATFHNEDERWAAVLKRDMSADGAFFYGVRTTGVFCRPSCASRLPRRENVDFFESADDARAAGYRECKRCQ
PGGLPRELEIVKRACATLDAYPQQRLTLAQLSDAVHISPFHLQRLFKRVVGVSPRQYQAAQRGAAFRDALQRGADVTRAT
VDAGFGSPSRMYDTAAAELGMAPSEYRRKGAGLRVRYASALTSLGFVLVATTDKGICKIAFGDDEAPLIDDLRQEFASAE
LLGDPVRLAPFIEQIDAYLQGKRERFDLPLDIAATAFQQRVWEALRRIPYGETRTYSQIAEAVGLPRAVRAVANACASNP
VALAIPCHRVVQKGGALAGYRWGTARKATLLDTEAQRESSTADEATPTTADMDHAA
>Mature_376_residues
MNTAFSHAVPATFHNEDERWAAVLKRDMSADGAFFYGVRTTGVFCRPSCASRLPRRENVDFFESADDARAAGYRECKRCQ
PGGLPRELEIVKRACATLDAYPQQRLTLAQLSDAVHISPFHLQRLFKRVVGVSPRQYQAAQRGAAFRDALQRGADVTRAT
VDAGFGSPSRMYDTAAAELGMAPSEYRRKGAGLRVRYASALTSLGFVLVATTDKGICKIAFGDDEAPLIDDLRQEFASAE
LLGDPVRLAPFIEQIDAYLQGKRERFDLPLDIAATAFQQRVWEALRRIPYGETRTYSQIAEAVGLPRAVRAVANACASNP
VALAIPCHRVVQKGGALAGYRWGTARKATLLDTEAQRESSTADEATPTTADMDHAA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=129, Percent_Identity=37.2093023255814, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788542, Length=351, Percent_Identity=47.5783475783476, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI1787596, Length=104, Percent_Identity=41.3461538461538, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=55.5555555555556, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=55.5555555555556, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=91, Percent_Identity=39.5604395604396, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137554, Length=177, Percent_Identity=35.5932203389831, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 41149; Mature: 41149

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAFSHAVPATFHNEDERWAAVLKRDMSADGAFFYGVRTTGVFCRPSCASRLPRRENVD
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHHHHCCCCCCCC
FFESADDARAAGYRECKRCQPGGLPRELEIVKRACATLDAYPQQRLTLAQLSDAVHISPF
HHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHH
HLQRLFKRVVGVSPRQYQAAQRGAAFRDALQRGADVTRATVDAGFGSPSRMYDTAAAELG
HHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHC
MAPSEYRRKGAGLRVRYASALTSLGFVLVATTDKGICKIAFGDDEAPLIDDLRQEFASAE
CCHHHHHHCCCCEEEHHHHHHHHCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
LLGDPVRLAPFIEQIDAYLQGKRERFDLPLDIAATAFQQRVWEALRRIPYGETRTYSQIA
HCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EAVGLPRAVRAVANACASNPVALAIPCHRVVQKGGALAGYRWGTARKATLLDTEAQRESS
HHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCEEEECCCCCCCEEEECCHHHHHCC
TADEATPTTADMDHAA
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTAFSHAVPATFHNEDERWAAVLKRDMSADGAFFYGVRTTGVFCRPSCASRLPRRENVD
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHHHHCCCCCCCC
FFESADDARAAGYRECKRCQPGGLPRELEIVKRACATLDAYPQQRLTLAQLSDAVHISPF
HHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHH
HLQRLFKRVVGVSPRQYQAAQRGAAFRDALQRGADVTRATVDAGFGSPSRMYDTAAAELG
HHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHC
MAPSEYRRKGAGLRVRYASALTSLGFVLVATTDKGICKIAFGDDEAPLIDDLRQEFASAE
CCHHHHHHCCCCEEEHHHHHHHHCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
LLGDPVRLAPFIEQIDAYLQGKRERFDLPLDIAATAFQQRVWEALRRIPYGETRTYSQIA
HCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EAVGLPRAVRAVANACASNPVALAIPCHRVVQKGGALAGYRWGTARKATLLDTEAQRESS
HHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCEEEECCCCCCCEEEECCHHHHHCC
TADEATPTTADMDHAA
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]