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The map label for this gene is alkA [H]

Identifier: 186477831

GI number: 186477831

Start: 3466171

End: 3467094

Strand: Reverse

Name: alkA [H]

Synonym: Bphy_3087

Alternate gene names: 186477831

Gene position: 3467094-3466171 (Counterclockwise)

Preceding gene: 186477832

Following gene: 186477830

Centisome position: 99.65

GC content: 71.0

Gene sequence:

>924_bases
TTGAGCACCACGCCGACAGCCGTACTCGAACTGCCGTTCAAAGCGCCCTATGACTGGCCGCGCATCCTGCGATTTTTCGG
CGGACGCGCGACGCCGGGCGTCGAAGCCGTCGAAGACGGCGTATGGCGTCGCGCGATCGGATGGAACGGCGACAGCGGGA
CGCTCACCGTCCGCAAGCATCCGCGCCGGCATTGCATCGTCGCCACGCTCGATGGCGCGGTAAGCCGCCATGCGGACGCG
CTCGCCGCGCCGATTGCCCGCATGTTCGATCTGCACGCCGACCCTCGATCGATCGGCGCAGCCCTTGCCGCCGATCCGTG
GCTCGCGCCGCTGGTCGCCGCGGCGCCCGGCCTTCGCGTGCCCGGCGCGTGGTCCGGGTTCGAACTCGTCGTGCGCGCTA
TCGTCGGCCAGCAGGTCAGCGTGAAGGCCGCGACGACGATCATCGGCCGAATCGTGCAGCGCGCCGGCGAGCGCATCGAC
GGTCATCCGCATGAAAGCACCGCCTGGCGCTTTCCGACGCCCGCCGCGCTCGCCGCCGTCGACCTCGCGGGCATCGGCAT
GCCGGGCAAGCGCATTCAGGCGCTGCAAGGATTCGCGCGCGCCGTGGCGGATGGGGAAGTGCCCATAGACAGCGTCGGCA
GCGCCTCCTTCGATCTTATCCAGCTGCGTGCGTCGCTGCTCGCGCTGCCGGGCATCGGTCCGTGGACCGTCGAATACGTC
GCGATGCGCGCCTGGCGCGACGCCGATGCGTGGCCCGCATGGGATCTCGTGCTGATGCAGGCCATCGCGGCCCGCGACCC
CGCGCTCGTGCGCCCCAATCAGCAGCGTGCCCGCACCGACGCCTGGCGGCCGTGGCGCGCCTACGCGGCGATGCATCTGT
GGAACGAAATCGCCGATCGCGCGGGTGCGGCACGCGGCGGCTAG

Upstream 100 bases:

>100_bases
GCACCGCGCGCAAGGCGACGCTGCTCGACACGGAAGCGCAGCGTGAATCCAGCACTGCCGACGAAGCCACCCCCACGACC
GCCGACATGGACCACGCCGC

Downstream 100 bases:

>100_bases
CGGTTTGCCGCACATTCAAGCCCGCGCCGGGAATCGACTTTGCGGCCTCGCGCGGCGTACAGTGAGAGAACGTGCACGAA
CCTCGCGAACCCCGCCGCAA

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase II, inducible; TAG II; DNA-3-methyladenine glycosidase II; DNA-3-methyladenine glycosylase II [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSTTPTAVLELPFKAPYDWPRILRFFGGRATPGVEAVEDGVWRRAIGWNGDSGTLTVRKHPRRHCIVATLDGAVSRHADA
LAAPIARMFDLHADPRSIGAALAADPWLAPLVAAAPGLRVPGAWSGFELVVRAIVGQQVSVKAATTIIGRIVQRAGERID
GHPHESTAWRFPTPAALAAVDLAGIGMPGKRIQALQGFARAVADGEVPIDSVGSASFDLIQLRASLLALPGIGPWTVEYV
AMRAWRDADAWPAWDLVLMQAIAARDPALVRPNQQRARTDAWRPWRAYAAMHLWNEIADRAGAARGG

Sequences:

>Translated_307_residues
MSTTPTAVLELPFKAPYDWPRILRFFGGRATPGVEAVEDGVWRRAIGWNGDSGTLTVRKHPRRHCIVATLDGAVSRHADA
LAAPIARMFDLHADPRSIGAALAADPWLAPLVAAAPGLRVPGAWSGFELVVRAIVGQQVSVKAATTIIGRIVQRAGERID
GHPHESTAWRFPTPAALAAVDLAGIGMPGKRIQALQGFARAVADGEVPIDSVGSASFDLIQLRASLLALPGIGPWTVEYV
AMRAWRDADAWPAWDLVLMQAIAARDPALVRPNQQRARTDAWRPWRAYAAMHLWNEIADRAGAARGG
>Mature_306_residues
STTPTAVLELPFKAPYDWPRILRFFGGRATPGVEAVEDGVWRRAIGWNGDSGTLTVRKHPRRHCIVATLDGAVSRHADAL
AAPIARMFDLHADPRSIGAALAADPWLAPLVAAAPGLRVPGAWSGFELVVRAIVGQQVSVKAATTIIGRIVQRAGERIDG
HPHESTAWRFPTPAALAAVDLAGIGMPGKRIQALQGFARAVADGEVPIDSVGSASFDLIQLRASLLALPGIGPWTVEYVA
MRAWRDADAWPAWDLVLMQAIAARDPALVRPNQQRARTDAWRPWRAYAAMHLWNEIADRAGAARGG

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=290, Percent_Identity=36.2068965517241, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 33016; Mature: 32885

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPTAVLELPFKAPYDWPRILRFFGGRATPGVEAVEDGVWRRAIGWNGDSGTLTVRKH
CCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCC
PRRHCIVATLDGAVSRHADALAAPIARMFDLHADPRSIGAALAADPWLAPLVAAAPGLRV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCC
PGAWSGFELVVRAIVGQQVSVKAATTIIGRIVQRAGERIDGHPHESTAWRFPTPAALAAV
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
DLAGIGMPGKRIQALQGFARAVADGEVPIDSVGSASFDLIQLRASLLALPGIGPWTVEYV
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
AMRAWRDADAWPAWDLVLMQAIAARDPALVRPNQQRARTDAWRPWRAYAAMHLWNEIADR
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
AGAARGG
CCCCCCC
>Mature Secondary Structure 
STTPTAVLELPFKAPYDWPRILRFFGGRATPGVEAVEDGVWRRAIGWNGDSGTLTVRKH
CCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCC
PRRHCIVATLDGAVSRHADALAAPIARMFDLHADPRSIGAALAADPWLAPLVAAAPGLRV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCC
PGAWSGFELVVRAIVGQQVSVKAATTIIGRIVQRAGERIDGHPHESTAWRFPTPAALAAV
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
DLAGIGMPGKRIQALQGFARAVADGEVPIDSVGSASFDLIQLRASLLALPGIGPWTVEYV
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
AMRAWRDADAWPAWDLVLMQAIAARDPALVRPNQQRARTDAWRPWRAYAAMHLWNEIADR
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
AGAARGG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6094528; 9097040; 9278503; 6389535; 3529081; 8706135; 8706136 [H]