| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is gshA [H]
Identifier: 186477830
GI number: 186477830
Start: 3464308
End: 3465951
Strand: Reverse
Name: gshA [H]
Synonym: Bphy_3086
Alternate gene names: 186477830
Gene position: 3465951-3464308 (Counterclockwise)
Preceding gene: 186477831
Following gene: 186477829
Centisome position: 99.62
GC content: 62.71
Gene sequence:
>1644_bases ATGGCGGCCGACCCTGTTCGACCCGCATCAATGCCATACACGACTTCCACCCGCACGACCGACGCGCTTCTGCATCGCCT GTCCGTGCTGACATCCGGCCCGCAGCGCGACGCGCTGGCAGCCGGTTTGCGCGGCATCGAAAAGGAAAGCTTGCGCGTGC TGTCCGACGGCCATCTCGCGATGACGCAGCATCCGCGCTCGCTCGGCTCGGCGCTCACGCATCCTTCGCTGACCACCGAT TATTCCGAAGCACTGCTCGAACTGATCACGCCCGCCGAGCAGGACGCCGCGGCCACCATCGAGCGTCTCGACACGCTGCA TCGCTTCGTCTATTCGGCCATCGGCGACGAACTGCTGTGGAACAACTCGATGCCCGGTCTCTTGCCCGAAGACGACGAGA TTCCTATTGCGCACTATGGCACGTCGAACATGGGCAGGCTGAAGTACGTGTATCGCGTCGGCCTCGCGCTGCGTTACGGA CGAACGATGCAATGCATCGCAGGCATCCACTACAACTATTCGTTGAACGAAGAAGTGTGGCGGCTGCTGCACGCCGACCA GAAATCGACGGCCAATGCGGTCGATTTCCAGTCCGATCGCTATCTCGCGCTGATCCGCAACTTCCGCCGTACGAGCTGGC TGCTGATGTATCTGTTCGGCGCGTCGCCGGCACTCGACAGGCGCTTCCTGCGCGGCCGCCAGCACACGCTCGAAACGTTC GACGCCGATACGCTCTATCGTCCCTACGCAACCAGTCTGCGCATGAGCGATCTCGGCTATTCGAACACGACCGCGCAAGC TGCGCTGCATGCCGACTACGACACGCTGCCCGGCTATCTCGACGCGCTCGCGAAAGCCGTGAGCCAGCCGTATCCGCAAT ACGAGGCAATCGGCACGCAGCGCGACGGCGAATGGGTGCAAATCAACACTAACGTGCTGCAGATCGAAAACGAGTTTTAC TCGACGATCCGCCCGAAGCGCGTCACCTATCCGGGCGAGCGCCCGCTGCATGCGCTTGCTGCACGCGGCGTGCAATACGT CGAAGTGCGCTGCATGGACATCGACCCGTTCGAACCGACGGGCATCGCGCTCGAAACGGCGCGTTTTCTCGACGCATATC TTCTGGTGTGCGCGCTGGACGACAGCGCTACGCTGCCGCCGCCCGCGTACTGCGAGGCCAACCAGAACTTCGGCCGCGTG ACGATGGAAGGGCGCAAGCCGGGCCTCGAACTGGTGCGCGGTGGACAGCCCGTGAAGATGGTCGACTGGGCCCACGAACT GTTCGCGAAAATTGATCACGCAGCGGCCGTGCTCGATTCGATTCGCGGTGACGACAGCCACGCGCGCTCGGTCGCAGCGC AACGCGCGAAGCTCGCGGATGCATCGTTGACGCCGTCTGCCCGTGTGCTGCAGATCATGCGCGACAAGCAGCAAAGCTTC CTCGAATTCGGCCTCGCGCAAAGCGTCGCGCATGCGGAGCATTTCCGCGCGCGTCCGCTGGACGAAGCGCAAGCGCTTGA GTTTGCCGCGCTGGCCGAGAAATCGCTGGCCGAACAAACGAAGCTCGAACGTGAAGAAGTCGGTTCGTTCGATGCGTTCG TCGCCGCATACCGCGCTTATACGTTGAACCGCTTCAGCGTTTGA
Upstream 100 bases:
>100_bases AGGCAACGCCAAGCAGCGGTTCTGGCGAGATGTTAAAGTGCCCGCTACTGAAAATTCCGCCGAACGTTGTCGACCGCGCT CCAGCAGCACCGTGCGGCGC
Downstream 100 bases:
>100_bases TGCGGTCGTGTTCGGGGCACTAGCCTCGCGCCCGACGAATGGCGAAAGGGCAGCTTTCACGCTGCCCTTTTTTCGTGGCG CGCGGCGCAGGGCCGGAAAT
Product: glutamate--cysteine ligase
Products: NA
Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]
Number of amino acids: Translated: 547; Mature: 546
Protein sequence:
>547_residues MAADPVRPASMPYTTSTRTTDALLHRLSVLTSGPQRDALAAGLRGIEKESLRVLSDGHLAMTQHPRSLGSALTHPSLTTD YSEALLELITPAEQDAAATIERLDTLHRFVYSAIGDELLWNNSMPGLLPEDDEIPIAHYGTSNMGRLKYVYRVGLALRYG RTMQCIAGIHYNYSLNEEVWRLLHADQKSTANAVDFQSDRYLALIRNFRRTSWLLMYLFGASPALDRRFLRGRQHTLETF DADTLYRPYATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPQYEAIGTQRDGEWVQINTNVLQIENEFY STIRPKRVTYPGERPLHALAARGVQYVEVRCMDIDPFEPTGIALETARFLDAYLLVCALDDSATLPPPAYCEANQNFGRV TMEGRKPGLELVRGGQPVKMVDWAHELFAKIDHAAAVLDSIRGDDSHARSVAAQRAKLADASLTPSARVLQIMRDKQQSF LEFGLAQSVAHAEHFRARPLDEAQALEFAALAEKSLAEQTKLEREEVGSFDAFVAAYRAYTLNRFSV
Sequences:
>Translated_547_residues MAADPVRPASMPYTTSTRTTDALLHRLSVLTSGPQRDALAAGLRGIEKESLRVLSDGHLAMTQHPRSLGSALTHPSLTTD YSEALLELITPAEQDAAATIERLDTLHRFVYSAIGDELLWNNSMPGLLPEDDEIPIAHYGTSNMGRLKYVYRVGLALRYG RTMQCIAGIHYNYSLNEEVWRLLHADQKSTANAVDFQSDRYLALIRNFRRTSWLLMYLFGASPALDRRFLRGRQHTLETF DADTLYRPYATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPQYEAIGTQRDGEWVQINTNVLQIENEFY STIRPKRVTYPGERPLHALAARGVQYVEVRCMDIDPFEPTGIALETARFLDAYLLVCALDDSATLPPPAYCEANQNFGRV TMEGRKPGLELVRGGQPVKMVDWAHELFAKIDHAAAVLDSIRGDDSHARSVAAQRAKLADASLTPSARVLQIMRDKQQSF LEFGLAQSVAHAEHFRARPLDEAQALEFAALAEKSLAEQTKLEREEVGSFDAFVAAYRAYTLNRFSV >Mature_546_residues AADPVRPASMPYTTSTRTTDALLHRLSVLTSGPQRDALAAGLRGIEKESLRVLSDGHLAMTQHPRSLGSALTHPSLTTDY SEALLELITPAEQDAAATIERLDTLHRFVYSAIGDELLWNNSMPGLLPEDDEIPIAHYGTSNMGRLKYVYRVGLALRYGR TMQCIAGIHYNYSLNEEVWRLLHADQKSTANAVDFQSDRYLALIRNFRRTSWLLMYLFGASPALDRRFLRGRQHTLETFD ADTLYRPYATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPQYEAIGTQRDGEWVQINTNVLQIENEFYS TIRPKRVTYPGERPLHALAARGVQYVEVRCMDIDPFEPTGIALETARFLDAYLLVCALDDSATLPPPAYCEANQNFGRVT MEGRKPGLELVRGGQPVKMVDWAHELFAKIDHAAAVLDSIRGDDSHARSVAAQRAKLADASLTPSARVLQIMRDKQQSFL EFGLAQSVAHAEHFRARPLDEAQALEFAALAEKSLAEQTKLEREEVGSFDAFVAAYRAYTLNRFSV
Specific function: Glutathione biosynthesis; first step. [C]
COG id: COG2918
COG function: function code H; Gamma-glutamylcysteine synthetase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1789044, Length=514, Percent_Identity=41.6342412451362, Blast_Score=369, Evalue=1e-103,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR007370 - InterPro: IPR006334 [H]
Pfam domain/function: PF04262 Glu_cys_ligase [H]
EC number: =6.3.2.2 [H]
Molecular weight: Translated: 61037; Mature: 60905
Theoretical pI: Translated: 6.10; Mature: 6.10
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAADPVRPASMPYTTSTRTTDALLHRLSVLTSGPQRDALAAGLRGIEKESLRVLSDGHLA CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCEE MTQHPRSLGSALTHPSLTTDYSEALLELITPAEQDAAATIERLDTLHRFVYSAIGDELLW ECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHC NNSMPGLLPEDDEIPIAHYGTSNMGRLKYVYRVGLALRYGRTMQCIAGIHYNYSLNEEVW CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHH RLLHADQKSTANAVDFQSDRYLALIRNFRRTSWLLMYLFGASPALDRRFLRGRQHTLETF HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC DADTLYRPYATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPQYEAIGTQ CCCCCCCCHHHHEEHHHCCCCCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCHHHCCCC RDGEWVQINTNVLQIENEFYSTIRPKRVTYPGERPLHALAARGVQYVEVRCMDIDPFEPT CCCCEEEEECCEEEECHHHHHHCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCC GIALETARFLDAYLLVCALDDSATLPPPAYCEANQNFGRVTMEGRKPGLELVRGGQPVKM CCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCHH VDWAHELFAKIDHAAAVLDSIRGDDSHARSVAAQRAKLADASLTPSARVLQIMRDKQQSF HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH LEFGLAQSVAHAEHFRARPLDEAQALEFAALAEKSLAEQTKLEREEVGSFDAFVAAYRAY HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH TLNRFSV HHHHCCC >Mature Secondary Structure AADPVRPASMPYTTSTRTTDALLHRLSVLTSGPQRDALAAGLRGIEKESLRVLSDGHLA CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCEE MTQHPRSLGSALTHPSLTTDYSEALLELITPAEQDAAATIERLDTLHRFVYSAIGDELLW ECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHC NNSMPGLLPEDDEIPIAHYGTSNMGRLKYVYRVGLALRYGRTMQCIAGIHYNYSLNEEVW CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHH RLLHADQKSTANAVDFQSDRYLALIRNFRRTSWLLMYLFGASPALDRRFLRGRQHTLETF HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC DADTLYRPYATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPQYEAIGTQ CCCCCCCCHHHHEEHHHCCCCCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCHHHCCCC RDGEWVQINTNVLQIENEFYSTIRPKRVTYPGERPLHALAARGVQYVEVRCMDIDPFEPT CCCCEEEEECCEEEECHHHHHHCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCC GIALETARFLDAYLLVCALDDSATLPPPAYCEANQNFGRVTMEGRKPGLELVRGGQPVKM CCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCHH VDWAHELFAKIDHAAAVLDSIRGDDSHARSVAAQRAKLADASLTPSARVLQIMRDKQQSF HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH LEFGLAQSVAHAEHFRARPLDEAQALEFAALAEKSLAEQTKLEREEVGSFDAFVAAYRAY HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH TLNRFSV HHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA