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The map label for this gene is livM [H]

Identifier: 186477790

GI number: 186477790

Start: 3421333

End: 3422304

Strand: Reverse

Name: livM [H]

Synonym: Bphy_3045

Alternate gene names: 186477790

Gene position: 3422304-3421333 (Counterclockwise)

Preceding gene: 186477791

Following gene: 186477789

Centisome position: 98.37

GC content: 63.27

Gene sequence:

>972_bases
ATGCAGAGAAAAGCGCTCTACGTTCTGCTGCTGATCGCGCTCATCGTTGCGCCGTTCGCCGGCGCGTATCCCGTGTTCGT
GATGAAGGTGCTGTGCTTCGCGCTGTTCGCGGGGGCCTTCAATCTGTTGATCGGTTATACGGGGCTTCTGTCGTTCGGTC
ACGCGATGTTTCTTGCGACGGCTGGCTATGTCACCGGCTACGCGATCCAGACGCTCGCGCTGTCGCCTGAACTCGGCGTA
TTGGCAGGGACGGCGGCGGCGACGGCGCTCGGCCTCGTCGTCGGACTGTTCGCAATCCGCAGGCAGGGCATCTACTTTGC
AATGGTCACGCTGGCGCTCGCGCAGATGGTCTACTTCGTGTTTCTGCAGGCGCCGTTCACGCACGGCGAGGACGGGCTTC
AAGGTGTGCCGCGAGGCAAGCTGTTCGGCGTGCTGAGCCTCTCGTCGGACCTGACGCTGTACTTCGTCGTGCTCGCGGTG
ATGGTGCTGGCGTTTCTGCTGATCGTGCGGATCGTGCATTCGCCGTTCGGGCAGGTGCTGATCGCGATCAAGGAGAATGA
GCCGCGCGCTATCTCGCTCGGCTATGATACGAACCGCTTCAAGCTGCTCGCGTTCATTCTGTCAGCGGGTCTGGCAGGCC
TTGCGGGCTCGCTCAAGGTGATCGTGCTGGGCTTCGAGACGCTCGGCGATGCGTACTGGACGATGTCAGGTCTCGTCGTG
CTGATGACGCTGGTGGGCGGCATGGGCACGCTGTTCGGGCCGCTGCTCGGTGCGGCGCTCATCGTTGCGCTCGAAGACCG
GCTTGGCGATATCGGCAGCGGGCTCGCGTCGATGACGGGCGTCGAATGGTTCCGCTCGCTTGGCGAGTCCGCGACAATCG
TGACGGGCCTCATCTTCATCGCATGCGTGCTGGCATTTCGGCGCGGCATCGTCGGCGAGATCGTGCAGCGGGTTAAACCG
CTGCGAGCGTGA

Upstream 100 bases:

>100_bases
CGCGCGTGTTCTATCCGGAAGCCTCGGCGACCGTGGTCTTCGTAATCATGGCGCTCGTTTTGCTCGTGCGTCCGGCGGGT
CTCTTCGGCAAGGAAAAATG

Downstream 100 bases:

>100_bases
GCAAGACGCGAATCTGATTGGGCCTCCGAAATAACGGCTTGCTGCTGTGAAATGATGCGAACGCGGAGCGAAACTACAGG
AACGCGCCCCAATTTTGTGC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQRKALYVLLLIALIVAPFAGAYPVFVMKVLCFALFAGAFNLLIGYTGLLSFGHAMFLATAGYVTGYAIQTLALSPELGV
LAGTAAATALGLVVGLFAIRRQGIYFAMVTLALAQMVYFVFLQAPFTHGEDGLQGVPRGKLFGVLSLSSDLTLYFVVLAV
MVLAFLLIVRIVHSPFGQVLIAIKENEPRAISLGYDTNRFKLLAFILSAGLAGLAGSLKVIVLGFETLGDAYWTMSGLVV
LMTLVGGMGTLFGPLLGAALIVALEDRLGDIGSGLASMTGVEWFRSLGESATIVTGLIFIACVLAFRRGIVGEIVQRVKP
LRA

Sequences:

>Translated_323_residues
MQRKALYVLLLIALIVAPFAGAYPVFVMKVLCFALFAGAFNLLIGYTGLLSFGHAMFLATAGYVTGYAIQTLALSPELGV
LAGTAAATALGLVVGLFAIRRQGIYFAMVTLALAQMVYFVFLQAPFTHGEDGLQGVPRGKLFGVLSLSSDLTLYFVVLAV
MVLAFLLIVRIVHSPFGQVLIAIKENEPRAISLGYDTNRFKLLAFILSAGLAGLAGSLKVIVLGFETLGDAYWTMSGLVV
LMTLVGGMGTLFGPLLGAALIVALEDRLGDIGSGLASMTGVEWFRSLGESATIVTGLIFIACVLAFRRGIVGEIVQRVKP
LRA
>Mature_323_residues
MQRKALYVLLLIALIVAPFAGAYPVFVMKVLCFALFAGAFNLLIGYTGLLSFGHAMFLATAGYVTGYAIQTLALSPELGV
LAGTAAATALGLVVGLFAIRRQGIYFAMVTLALAQMVYFVFLQAPFTHGEDGLQGVPRGKLFGVLSLSSDLTLYFVVLAV
MVLAFLLIVRIVHSPFGQVLIAIKENEPRAISLGYDTNRFKLLAFILSAGLAGLAGSLKVIVLGFETLGDAYWTMSGLVV
LMTLVGGMGTLFGPLLGAALIVALEDRLGDIGSGLASMTGVEWFRSLGESATIVTGLIFIACVLAFRRGIVGEIVQRVKP
LRA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=262, Percent_Identity=28.2442748091603, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34180; Mature: 34180

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKALYVLLLIALIVAPFAGAYPVFVMKVLCFALFAGAFNLLIGYTGLLSFGHAMFLAT
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGYVTGYAIQTLALSPELGVLAGTAAATALGLVVGLFAIRRQGIYFAMVTLALAQMVYFV
HHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FLQAPFTHGEDGLQGVPRGKLFGVLSLSSDLTLYFVVLAVMVLAFLLIVRIVHSPFGQVL
HHHCCCCCCCCCCCCCCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
IAIKENEPRAISLGYDTNRFKLLAFILSAGLAGLAGSLKVIVLGFETLGDAYWTMSGLVV
EEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHH
LMTLVGGMGTLFGPLLGAALIVALEDRLGDIGSGLASMTGVEWFRSLGESATIVTGLIFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ACVLAFRRGIVGEIVQRVKPLRA
HHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQRKALYVLLLIALIVAPFAGAYPVFVMKVLCFALFAGAFNLLIGYTGLLSFGHAMFLAT
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGYVTGYAIQTLALSPELGVLAGTAAATALGLVVGLFAIRRQGIYFAMVTLALAQMVYFV
HHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FLQAPFTHGEDGLQGVPRGKLFGVLSLSSDLTLYFVVLAVMVLAFLLIVRIVHSPFGQVL
HHHCCCCCCCCCCCCCCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
IAIKENEPRAISLGYDTNRFKLLAFILSAGLAGLAGSLKVIVLGFETLGDAYWTMSGLVV
EEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHH
LMTLVGGMGTLFGPLLGAALIVALEDRLGDIGSGLASMTGVEWFRSLGESATIVTGLIFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ACVLAFRRGIVGEIVQRVKPLRA
HHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]