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The map label for this gene is gidB [H]

Identifier: 186477784

GI number: 186477784

Start: 3411615

End: 3412292

Strand: Reverse

Name: gidB [H]

Synonym: Bphy_3038

Alternate gene names: 186477784

Gene position: 3412292-3411615 (Counterclockwise)

Preceding gene: 186477785

Following gene: 186477783

Centisome position: 98.08

GC content: 58.55

Gene sequence:

>678_bases
ATGACGGCGCGGTCGGATGCAGTGGTGAGCCGTCAAGCGCTCGTCGGTTTGCTGGATGAGGGCGTGCATGAGTTGGGATT
CGACCTTCGCGCAGATCAGAAAGGCAAACTGCTCGACTATGTCGCGTTGCTGGCCAAGTGGAATGCCGTCTACAACCTGA
CGGCGATCCGCGATCCGCGGCAAATGCTAATTCAGCACATCTTGGACTCGCTCTCGATTGTTCCGCATCTGGCTTCGCTC
GAACGGGCGTCGGCGCTTGATGTCGGCTCAGGCGGTGGCCTGCCGGGTATCGTGCTGGCGATCGTATTCCCCGCATGGTC
GATCACGGTCAACGACATCGTCCAGAAAAAGTCCGCCTTTCAGTCCCAAGTCAAAGCTGAACTCGGCCTGACGAATCTGT
CCGTGGTGACCGGACGTGTTGAAAGATTGCAGCCAGGCGTAGAGGTCCCGGGAAAGTTTGATGTGATCGTCTCGCGCGCA
TTCGCAGAACTCGCGGATTTCGTTACACTGGCCCGTCACCTCGTTGCTGACAATGGTGCGATCTGGGCGATGAAGGGCGT
GCGACCCGATAAGGAAATCGAGCGCTTGCCCGAAGGCGCACAGGTCAAGCAGGTGATTCGTCTGAAAGTGCCATCGCTGG
ATGCCGAACGGCATCTGGTCGAAGTCGCGGTAGGCTGA

Upstream 100 bases:

>100_bases
CGGCCGCGATCTCCTTGTTGATGGTTCACCTCAAGCGCGGCCTAGGTCGGCGAGCGCCCAGGCCTGCTGGCGCCGGTAAC
GACAACTCTCCGGTGGCTCA

Downstream 100 bases:

>100_bases
ATTCCGATTGGGATTTCAATTAAACAGGCTGCAAAGGGGCACCTAAAAGATGGCAAAAATCTTCTGCGTTGCGAACCAGA
AGGGTGGAGTCGGCAAAACG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTARSDAVVSRQALVGLLDEGVHELGFDLRADQKGKLLDYVALLAKWNAVYNLTAIRDPRQMLIQHILDSLSIVPHLASL
ERASALDVGSGGGLPGIVLAIVFPAWSITVNDIVQKKSAFQSQVKAELGLTNLSVVTGRVERLQPGVEVPGKFDVIVSRA
FAELADFVTLARHLVADNGAIWAMKGVRPDKEIERLPEGAQVKQVIRLKVPSLDAERHLVEVAVG

Sequences:

>Translated_225_residues
MTARSDAVVSRQALVGLLDEGVHELGFDLRADQKGKLLDYVALLAKWNAVYNLTAIRDPRQMLIQHILDSLSIVPHLASL
ERASALDVGSGGGLPGIVLAIVFPAWSITVNDIVQKKSAFQSQVKAELGLTNLSVVTGRVERLQPGVEVPGKFDVIVSRA
FAELADFVTLARHLVADNGAIWAMKGVRPDKEIERLPEGAQVKQVIRLKVPSLDAERHLVEVAVG
>Mature_224_residues
TARSDAVVSRQALVGLLDEGVHELGFDLRADQKGKLLDYVALLAKWNAVYNLTAIRDPRQMLIQHILDSLSIVPHLASLE
RASALDVGSGGGLPGIVLAIVFPAWSITVNDIVQKKSAFQSQVKAELGLTNLSVVTGRVERLQPGVEVPGKFDVIVSRAF
AELADFVTLARHLVADNGAIWAMKGVRPDKEIERLPEGAQVKQVIRLKVPSLDAERHLVEVAVG

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=197, Percent_Identity=47.2081218274112, Blast_Score=171, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 24349; Mature: 24217

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARSDAVVSRQALVGLLDEGVHELGFDLRADQKGKLLDYVALLAKWNAVYNLTAIRDPR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEHCCCCHH
QMLIQHILDSLSIVPHLASLERASALDVGSGGGLPGIVLAIVFPAWSITVNDIVQKKSAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSQVKAELGLTNLSVVTGRVERLQPGVEVPGKFDVIVSRAFAELADFVTLARHLVADNGA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IWAMKGVRPDKEIERLPEGAQVKQVIRLKVPSLDAERHLVEVAVG
EEEECCCCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHEEEEECC
>Mature Secondary Structure 
TARSDAVVSRQALVGLLDEGVHELGFDLRADQKGKLLDYVALLAKWNAVYNLTAIRDPR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEHCCCCHH
QMLIQHILDSLSIVPHLASLERASALDVGSGGGLPGIVLAIVFPAWSITVNDIVQKKSAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSQVKAELGLTNLSVVTGRVERLQPGVEVPGKFDVIVSRAFAELADFVTLARHLVADNGA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IWAMKGVRPDKEIERLPEGAQVKQVIRLKVPSLDAERHLVEVAVG
EEEECCCCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA