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The map label for this gene is tag [H]

Identifier: 186477731

GI number: 186477731

Start: 3343206

End: 3343802

Strand: Direct

Name: tag [H]

Synonym: Bphy_2985

Alternate gene names: 186477731

Gene position: 3343206-3343802 (Clockwise)

Preceding gene: 186477728

Following gene: 186477732

Centisome position: 96.09

GC content: 59.8

Gene sequence:

>597_bases
GTGGCTGAGCAGCGATGCAACTGGGCGACGAGCGAAGCGCTCGCACACTATCACGACACCGAGTGGGGTGTCCCTTCGCG
CGACGACCGGCATCTGTTCGAGATGCTCGTGCTCGAAGGCGCGCAGGCAGGGCTGTCGTGGTCGACGATCCTGAACAAGC
GCGCCGGCTATCGGCGTGCATTCTCGAATTTCGATATCGATAAGGTCGCCCGCTACTCCGCGCAAAAGATCGACGCGCTC
GTGCTCGATGAGGGTATCGTGCGCCATCGCGGCAAGATTGAGTCGACGGTTCTCAATGCGAAAGCGGTCAGGCAGATTCA
GGCGGAGCACGGTTCGCTAGCGGATTTCGTATGGTCATTCGTCGATAACGTGCCCATCCAGAATACGTGGACGAGCTACC
AGCACGCGCCCGCATCGACGGAAGTGTCGGACATGCTCAGCAAGGCGCTCAAAGGTTATGGCTGCAAATTTGTGGGCACA
ACGATCTGCTACGCCTTCATGCAGGCAGTCGGCATGGTCAACGACCACCAGACCGATTGCGCCTGCTATTCGCGCTGCGC
GTCGCTCGGTGAGAAGCGGCGCAAGAAGACGAAGTGA

Upstream 100 bases:

>100_bases
CGACAATTAGCCGATCGGCCAGGTTGCGGCTGCACCCCATTCCGCTAAGCTGGCGCGATCCGATCGACGGGCGATCGACC
AACCATGCAGGAGCCTGAAA

Downstream 100 bases:

>100_bases
TGCGTCGCGGGCCATATGCCGACCGCGATCCGCTGGTGCGCCGTTGCAACACGTGATGTCTCGGCGCGCCAGCTCGAAGG
GGTGCGAGAGCTCGCCCGGG

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAEQRCNWATSEALAHYHDTEWGVPSRDDRHLFEMLVLEGAQAGLSWSTILNKRAGYRRAFSNFDIDKVARYSAQKIDAL
VLDEGIVRHRGKIESTVLNAKAVRQIQAEHGSLADFVWSFVDNVPIQNTWTSYQHAPASTEVSDMLSKALKGYGCKFVGT
TICYAFMQAVGMVNDHQTDCACYSRCASLGEKRRKKTK

Sequences:

>Translated_198_residues
MAEQRCNWATSEALAHYHDTEWGVPSRDDRHLFEMLVLEGAQAGLSWSTILNKRAGYRRAFSNFDIDKVARYSAQKIDAL
VLDEGIVRHRGKIESTVLNAKAVRQIQAEHGSLADFVWSFVDNVPIQNTWTSYQHAPASTEVSDMLSKALKGYGCKFVGT
TICYAFMQAVGMVNDHQTDCACYSRCASLGEKRRKKTK
>Mature_197_residues
AEQRCNWATSEALAHYHDTEWGVPSRDDRHLFEMLVLEGAQAGLSWSTILNKRAGYRRAFSNFDIDKVARYSAQKIDALV
LDEGIVRHRGKIESTVLNAKAVRQIQAEHGSLADFVWSFVDNVPIQNTWTSYQHAPASTEVSDMLSKALKGYGCKFVGTT
ICYAFMQAVGMVNDHQTDCACYSRCASLGEKRRKKTK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=182, Percent_Identity=52.1978021978022, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 22271; Mature: 22140

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQRCNWATSEALAHYHDTEWGVPSRDDRHLFEMLVLEGAQAGLSWSTILNKRAGYRRA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FSNFDIDKVARYSAQKIDALVLDEGIVRHRGKIESTVLNAKAVRQIQAEHGSLADFVWSF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VDNVPIQNTWTSYQHAPASTEVSDMLSKALKGYGCKFVGTTICYAFMQAVGMVNDHQTDC
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHH
ACYSRCASLGEKRRKKTK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEQRCNWATSEALAHYHDTEWGVPSRDDRHLFEMLVLEGAQAGLSWSTILNKRAGYRRA
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FSNFDIDKVARYSAQKIDALVLDEGIVRHRGKIESTVLNAKAVRQIQAEHGSLADFVWSF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VDNVPIQNTWTSYQHAPASTEVSDMLSKALKGYGCKFVGTTICYAFMQAVGMVNDHQTDC
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHH
ACYSRCASLGEKRRKKTK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]