| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is slt [H]
Identifier: 186477648
GI number: 186477648
Start: 3254432
End: 3256405
Strand: Reverse
Name: slt [H]
Synonym: Bphy_2900
Alternate gene names: 186477648
Gene position: 3256405-3254432 (Counterclockwise)
Preceding gene: 186477650
Following gene: 186477647
Centisome position: 93.6
GC content: 64.69
Gene sequence:
>1974_bases ATGTCCAAACGCCTTTTCCGAGTATATCGCGCGGCCAGTCTGGCGCTCGCCGCGGCGGCACTCGTCGCGTGCAGCACGGC CTCCGCGGTCAAGCCCATCCAGCTCTCCCAACTCACCAACGACGACCAGATTTTCGTGCAGTTGCGCGAGGCAGCGCGCA ACAACGACGCAGGCCGCGCTGCGCAACTCGCGGCGATGATTCCGGACTATCCGGCGCCCGCGTACCTCGAATACTTCCAG CTGAAGCCGCAACTGTTCGACGGCTCGGGCCATCCGCGCATCGACGCAGCCGACGCACCCGTGCTGTCGTTCTTGCAACG CTACGACGGCCAGGCCATCGCCGACCGCATGCGCAACGACTACCTGGTCGTGCTCGGCGCACGACACGACTGGCGCAACT TCGACCAGCAATACGCGCGCTTCGTGCTGAACGACGACACCCAGGTGAAGTGCTACGCGCTCGAATCGCGCGCGGCGAAA GGCGAGAACGTCGTCGACGCAGCGCGCGCGCTGCTCGTCGAGCCGCGCTATTACGGCGACGGCTGCGTCGATCTCATCAC CTCGCTGGCGATCAGCCAGCAGTTCACGACCGACGACGTATGGCAGCAGATCCGTCTCGCGTACGAACAGAACCAGACCA GCACGGGCGCGAAGCTCGTCGACGCACTGGGCAACAACCGTCCCGATCCGACGCTGTTCGATCAGGCCGCAACTACGCCG CCGTTGCTGCTCGCGCGCGGTGTCGGCGCCGATCCGCAATCGCATCAGCTCGCGCTGCTCGCGATCACGCGCATGGCCCG CAACGATCCCGCCATGGCAGCCGCCACCTTCGGCTCGGTCGCACCTTCGCTGACGTCGCCCGAACGCGGGATCGGCTGGG GCACGATTGCGTATCAGGCCGCTGCGAAGCAGATGCCGGGCGCCGTCGACTGGTATCGCCTGTCGGCGAATGCACCGCTG TCCACGCCCGCTTACGAATGGCGCACGCGCGTCGCGCTTCTTGCCGGCGACTGGACGATGGTGCGCTGGTCGATCGAGCA AATGCCGCCTTCATTGCGCAGCCAGCCCGCGTGGGCCTACTGGCACGCGCGCGCTATCAAGGCGGCGGGCGACGTGACGA CGGCCAATCAGGAATTCGAGTCGATCGCGCAGCAGTACAACTTCTACGGCCAGCTTGCCGCCGAAGAACTCGGCCAGAAG ATCGTCGTGCCGCCGAAAACCACCGTCACCGATGCCGAAGTGCAACAGGCGGGCGACACGCCCGGCTTCGCGCTTGCCCA GAAGTTCTATCAGCTGAATCTGCGGCTCGAAGGCAACCGCGAATGGAACTGGCCGTTGCGCAGCATGACCGACCGTCAAC TGATCGCCGCCGCCGAATATGCGCGCCGTATCGAGCTCTATGACCGCGCAGTGAATACGGCCGATCGCACGAAGACCGAG CACGACTTCTCGCTGCGCTTCCTGTCGCCGTTCCGCGATATCGTCGAGCGCGACGCGCAGTCGAACGGGCTCGATGTCGA ATGGGCGTATGGTCTGATCCGTCAGGAATCGCGCTTCATCATGAACGCGCGCTCGGAGGTCGGCGCGAGCGGCCTGATGC AGCTGATGCCGGGCACGGCGCAACTGGTCGCGAAGAAGATCGGCCTCGGCACCATCTCGCGTGCCCAGATGAACGACATC AACACCAATATCCTGCTCGGCACGAACTATCTGTCGATGATCTACAATGAGTTCGACGGCTCCGCCGTGCTCGCCACGGC GGGCTACAACGCAGGTCCGGGCCGTCCGCGCAACTGGCGCGCGTCGTTGCCGCGCGGTGTCGAAGGCGCGATTTTCGCGG AAGCGATTCCGTTCCAGGAAACGCGCGACTACGTGAAGAACGTGCTGTCCAACACCGTCTACTATGCGGCGTTGTTCGAA GGCCGTCCGCAATCGCTGAAGGCGCGTCTGGGTTATATCCAGCCGTTGCAGTAA
Upstream 100 bases:
>100_bases TTCCAGCAGTGTTGCGCGCAGGTCCTTTTTCGATTTCGCCGCGGGGTTGCATGCTATGCTTGAGTTCAATTCGCGCTCCA GAAACGCTCTAGAAATAACG
Downstream 100 bases:
>100_bases CGATGCGCCATGCCGCCGCCGCGTGACCCGTCACGCAAGCGGCGGCGCTCGAACCTCAGCCGTTGCCGCGGCTTCCATCA GGGAGTCGAAAAATGCGACA
Product: lytic transglycosylase
Products: 1,6-Anhydrobond [C]
Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]
Number of amino acids: Translated: 657; Mature: 656
Protein sequence:
>657_residues MSKRLFRVYRAASLALAAAALVACSTASAVKPIQLSQLTNDDQIFVQLREAARNNDAGRAAQLAAMIPDYPAPAYLEYFQ LKPQLFDGSGHPRIDAADAPVLSFLQRYDGQAIADRMRNDYLVVLGARHDWRNFDQQYARFVLNDDTQVKCYALESRAAK GENVVDAARALLVEPRYYGDGCVDLITSLAISQQFTTDDVWQQIRLAYEQNQTSTGAKLVDALGNNRPDPTLFDQAATTP PLLLARGVGADPQSHQLALLAITRMARNDPAMAAATFGSVAPSLTSPERGIGWGTIAYQAAAKQMPGAVDWYRLSANAPL STPAYEWRTRVALLAGDWTMVRWSIEQMPPSLRSQPAWAYWHARAIKAAGDVTTANQEFESIAQQYNFYGQLAAEELGQK IVVPPKTTVTDAEVQQAGDTPGFALAQKFYQLNLRLEGNREWNWPLRSMTDRQLIAAAEYARRIELYDRAVNTADRTKTE HDFSLRFLSPFRDIVERDAQSNGLDVEWAYGLIRQESRFIMNARSEVGASGLMQLMPGTAQLVAKKIGLGTISRAQMNDI NTNILLGTNYLSMIYNEFDGSAVLATAGYNAGPGRPRNWRASLPRGVEGAIFAEAIPFQETRDYVKNVLSNTVYYAALFE GRPQSLKARLGYIQPLQ
Sequences:
>Translated_657_residues MSKRLFRVYRAASLALAAAALVACSTASAVKPIQLSQLTNDDQIFVQLREAARNNDAGRAAQLAAMIPDYPAPAYLEYFQ LKPQLFDGSGHPRIDAADAPVLSFLQRYDGQAIADRMRNDYLVVLGARHDWRNFDQQYARFVLNDDTQVKCYALESRAAK GENVVDAARALLVEPRYYGDGCVDLITSLAISQQFTTDDVWQQIRLAYEQNQTSTGAKLVDALGNNRPDPTLFDQAATTP PLLLARGVGADPQSHQLALLAITRMARNDPAMAAATFGSVAPSLTSPERGIGWGTIAYQAAAKQMPGAVDWYRLSANAPL STPAYEWRTRVALLAGDWTMVRWSIEQMPPSLRSQPAWAYWHARAIKAAGDVTTANQEFESIAQQYNFYGQLAAEELGQK IVVPPKTTVTDAEVQQAGDTPGFALAQKFYQLNLRLEGNREWNWPLRSMTDRQLIAAAEYARRIELYDRAVNTADRTKTE HDFSLRFLSPFRDIVERDAQSNGLDVEWAYGLIRQESRFIMNARSEVGASGLMQLMPGTAQLVAKKIGLGTISRAQMNDI NTNILLGTNYLSMIYNEFDGSAVLATAGYNAGPGRPRNWRASLPRGVEGAIFAEAIPFQETRDYVKNVLSNTVYYAALFE GRPQSLKARLGYIQPLQ >Mature_656_residues SKRLFRVYRAASLALAAAALVACSTASAVKPIQLSQLTNDDQIFVQLREAARNNDAGRAAQLAAMIPDYPAPAYLEYFQL KPQLFDGSGHPRIDAADAPVLSFLQRYDGQAIADRMRNDYLVVLGARHDWRNFDQQYARFVLNDDTQVKCYALESRAAKG ENVVDAARALLVEPRYYGDGCVDLITSLAISQQFTTDDVWQQIRLAYEQNQTSTGAKLVDALGNNRPDPTLFDQAATTPP LLLARGVGADPQSHQLALLAITRMARNDPAMAAATFGSVAPSLTSPERGIGWGTIAYQAAAKQMPGAVDWYRLSANAPLS TPAYEWRTRVALLAGDWTMVRWSIEQMPPSLRSQPAWAYWHARAIKAAGDVTTANQEFESIAQQYNFYGQLAAEELGQKI VVPPKTTVTDAEVQQAGDTPGFALAQKFYQLNLRLEGNREWNWPLRSMTDRQLIAAAEYARRIELYDRAVNTADRTKTEH DFSLRFLSPFRDIVERDAQSNGLDVEWAYGLIRQESRFIMNARSEVGASGLMQLMPGTAQLVAKKIGLGTISRAQMNDIN TNILLGTNYLSMIYNEFDGSAVLATAGYNAGPGRPRNWRASLPRGVEGAIFAEAIPFQETRDYVKNVLSNTVYYAALFEG RPQSLKARLGYIQPLQ
Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]
COG id: COG0741
COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)
Gene ontology:
Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transglycosylase slt family [H]
Homologues:
Organism=Escherichia coli, GI87082441, Length=649, Percent_Identity=26.6563944530046, Blast_Score=180, Evalue=2e-46,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016026 - InterPro: IPR008258 - InterPro: IPR012289 - InterPro: IPR008939 - InterPro: IPR000189 [H]
Pfam domain/function: PF01464 SLT [H]
EC number: 3.2.1.- [C]
Molecular weight: Translated: 72776; Mature: 72645
Theoretical pI: Translated: 7.05; Mature: 7.05
Prosite motif: PS00013 PROKAR_LIPOPROTEIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSKRLFRVYRAASLALAAAALVACSTASAVKPIQLSQLTNDDQIFVQLREAARNNDAGRA CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCCHHHHHHHHHHHCCCCCCHH AQLAAMIPDYPAPAYLEYFQLKPQLFDGSGHPRIDAADAPVLSFLQRYDGQAIADRMRND HHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCC YLVVLGARHDWRNFDQQYARFVLNDDTQVKCYALESRAAKGENVVDAARALLVEPRYYGD EEEEEECCCCHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC GCVDLITSLAISQQFTTDDVWQQIRLAYEQNQTSTGAKLVDALGNNRPDPTLFDQAATTP HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCC PLLLARGVGADPQSHQLALLAITRMARNDPAMAAATFGSVAPSLTSPERGIGWGTIAYQA CHHEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHH AAKQMPGAVDWYRLSANAPLSTPAYEWRTRVALLAGDWTMVRWSIEQMPPSLRSQPAWAY HHHHCCCCCEEEEEECCCCCCCCCHHHHHEEEEEECCEEEEEEEHHHCCCCHHCCCCCHH WHARAIKAAGDVTTANQEFESIAQQYNFYGQLAAEELGQKIVVPPKTTVTDAEVQQAGDT HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHCCCC PGFALAQKFYQLNLRLEGNREWNWPLRSMTDRQLIAAAEYARRIELYDRAVNTADRTKTE CHHHHHHHHHEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC HDFSLRFLSPFRDIVERDAQSNGLDVEWAYGLIRQESRFIMNARSEVGASGLMQLMPGTA CCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHH QLVAKKIGLGTISRAQMNDINTNILLGTNYLSMIYNEFDGSAVLATAGYNAGPGRPRNWR HHHHHHHCCCCCCHHHHCCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC ASLPRGVEGAIFAEAIPFQETRDYVKNVLSNTVYYAALFEGRPQSLKARLGYIQPLQ CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCCCC >Mature Secondary Structure SKRLFRVYRAASLALAAAALVACSTASAVKPIQLSQLTNDDQIFVQLREAARNNDAGRA CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCCHHHHHHHHHHHCCCCCCHH AQLAAMIPDYPAPAYLEYFQLKPQLFDGSGHPRIDAADAPVLSFLQRYDGQAIADRMRND HHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCC YLVVLGARHDWRNFDQQYARFVLNDDTQVKCYALESRAAKGENVVDAARALLVEPRYYGD EEEEEECCCCHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC GCVDLITSLAISQQFTTDDVWQQIRLAYEQNQTSTGAKLVDALGNNRPDPTLFDQAATTP HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCC PLLLARGVGADPQSHQLALLAITRMARNDPAMAAATFGSVAPSLTSPERGIGWGTIAYQA CHHEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHH AAKQMPGAVDWYRLSANAPLSTPAYEWRTRVALLAGDWTMVRWSIEQMPPSLRSQPAWAY HHHHCCCCCEEEEEECCCCCCCCCHHHHHEEEEEECCEEEEEEEHHHCCCCHHCCCCCHH WHARAIKAAGDVTTANQEFESIAQQYNFYGQLAAEELGQKIVVPPKTTVTDAEVQQAGDT HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHCCCC PGFALAQKFYQLNLRLEGNREWNWPLRSMTDRQLIAAAEYARRIELYDRAVNTADRTKTE CHHHHHHHHHEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC HDFSLRFLSPFRDIVERDAQSNGLDVEWAYGLIRQESRFIMNARSEVGASGLMQLMPGTA CCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHH QLVAKKIGLGTISRAQMNDINTNILLGTNYLSMIYNEFDGSAVLATAGYNAGPGRPRNWR HHHHHHHCCCCCCHHHHCCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC ASLPRGVEGAIFAEAIPFQETRDYVKNVLSNTVYYAALFEGRPQSLKARLGYIQPLQ CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]
Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]
General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]