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The map label for this gene is hemB [H]

Identifier: 186477557

GI number: 186477557

Start: 3163275

End: 3164273

Strand: Direct

Name: hemB [H]

Synonym: Bphy_2809

Alternate gene names: 186477557

Gene position: 3163275-3164273 (Clockwise)

Preceding gene: 186477556

Following gene: 186477605

Centisome position: 90.92

GC content: 62.76

Gene sequence:

>999_bases
ATGAGCTTCTATCCGCATCACCGCCCGCGCCGCATGCGCCGGGACGACTTCTCGCGCCGCATGATGCGCGAAAACATCCT
CACCACCAATGATCTGATTTACCCCGTCTTCGTGATCGAAGGGACGAACGTGCGTCAGGCCGTACCGTCGATGCCGGGCG
TCGAGCGCGTATCGGTTGATCTGCTGATGGGTGTTGCCGAGCAATGCATGGAACTCGGTGTGCCGGTGCTGTCGCTCTTC
CCTGTGATCGAGCCGTCGCTGAAAACGCCCGACGGCCGTGAGGCAACCAACGCAACCGGTCTGATTCCGCGCGCCGTGTG
CGAACTGAAGAAGCATTTCCCCGAACTGGGCGTGCTGACCGACGTCGCGCTCGATCCGTACACGAGCCATGGCCAGGACG
GCGTGCTCGACGAGCATGGCTACGTAATCAACGACGAAACCGTCGACATCCTCGTCGAACAGGCGCAGACGCAGGCGCAG
GCGGGCGTCGACATCGTCGCGCCGTCCGACATGATGGATGGCCGGATCGGCGCGATCCGCGAGTTGCTCGAAAGCGAAGG
CCACATACACACGCGGATCATGGCGTACTCGGCGAAGTTCGCGTCCGCGTTCTACGGCCCGTTCCGCGATGCCGTGGGTT
CGGCAGCGAACCTCGGCAAGGGCAACAAGATGACGTATCAACTCGATCCCGCCAACTCGGACGAGGCGCTTCGCGAAGTG
CGCGCCGACATCGAAGAGGGCGCGGATATGGTGATGGTCAAGCCGGGCATGCCGTATCTGGACATCGTGCGTCGCGTAAA
GGACGAATTCCGCTTCCCGACGTACGTGTACCAGGTGAGCGGCGAGTACGCAATGCTGAAGGCCGCCGCGCAGAACGGCT
GGCTGGATCACGACAAGGTGATGATGGAATCGCTGCTCGCGTTCAAGCGCGCGGGCGCCGATGGCGTGTTGACGTATTTC
GCGCTGGACGCCGCGCGGTTGCTGCGAGCGCAGAAGTAA

Upstream 100 bases:

>100_bases
CAGCGCTAGGCGCCTTGCTCGATCGGTATGATATACCATTGTCTCGAAAAGTTTCTGGCAAGGCACGGCCAGCATTTTTG
ACTTTTGCAGATATCCTCAC

Downstream 100 bases:

>100_bases
GGGTCGTAGTCGCAGGCCGTGGCCGCGCATCGTTATGGTGCGACCGTCTTGCGACAGTGACTGCAAGCGGAGGGGTCGAC
CTGACAGCCTCCGCTTCTCT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSFYPHHRPRRMRRDDFSRRMMRENILTTNDLIYPVFVIEGTNVRQAVPSMPGVERVSVDLLMGVAEQCMELGVPVLSLF
PVIEPSLKTPDGREATNATGLIPRAVCELKKHFPELGVLTDVALDPYTSHGQDGVLDEHGYVINDETVDILVEQAQTQAQ
AGVDIVAPSDMMDGRIGAIRELLESEGHIHTRIMAYSAKFASAFYGPFRDAVGSAANLGKGNKMTYQLDPANSDEALREV
RADIEEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFKRAGADGVLTYF
ALDAARLLRAQK

Sequences:

>Translated_332_residues
MSFYPHHRPRRMRRDDFSRRMMRENILTTNDLIYPVFVIEGTNVRQAVPSMPGVERVSVDLLMGVAEQCMELGVPVLSLF
PVIEPSLKTPDGREATNATGLIPRAVCELKKHFPELGVLTDVALDPYTSHGQDGVLDEHGYVINDETVDILVEQAQTQAQ
AGVDIVAPSDMMDGRIGAIRELLESEGHIHTRIMAYSAKFASAFYGPFRDAVGSAANLGKGNKMTYQLDPANSDEALREV
RADIEEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFKRAGADGVLTYF
ALDAARLLRAQK
>Mature_331_residues
SFYPHHRPRRMRRDDFSRRMMRENILTTNDLIYPVFVIEGTNVRQAVPSMPGVERVSVDLLMGVAEQCMELGVPVLSLFP
VIEPSLKTPDGREATNATGLIPRAVCELKKHFPELGVLTDVALDPYTSHGQDGVLDEHGYVINDETVDILVEQAQTQAQA
GVDIVAPSDMMDGRIGAIRELLESEGHIHTRIMAYSAKFASAFYGPFRDAVGSAANLGKGNKMTYQLDPANSDEALREVR
ADIEEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFKRAGADGVLTYFA
LDAARLLRAQK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=309, Percent_Identity=44.336569579288, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI87081728, Length=325, Percent_Identity=45.5384615384615, Blast_Score=253, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321398, Length=306, Percent_Identity=38.8888888888889, Blast_Score=197, Evalue=1e-51,
Organism=Drosophila melanogaster, GI21358291, Length=295, Percent_Identity=41.6949152542373, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36928; Mature: 36797

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYPHHRPRRMRRDDFSRRMMRENILTTNDLIYPVFVIEGTNVRQAVPSMPGVERVSVD
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCCHHHHHHH
LLMGVAEQCMELGVPVLSLFPVIEPSLKTPDGREATNATGLIPRAVCELKKHFPELGVLT
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
DVALDPYTSHGQDGVLDEHGYVINDETVDILVEQAQTQAQAGVDIVAPSDMMDGRIGAIR
HHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEECCCHHHCCHHHHHH
ELLESEGHIHTRIMAYSAKFASAFYGPFRDAVGSAANLGKGNKMTYQLDPANSDEALREV
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEECCCCCHHHHHHH
RADIEEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKV
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHHH
MMESLLAFKRAGADGVLTYFALDAARLLRAQK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFYPHHRPRRMRRDDFSRRMMRENILTTNDLIYPVFVIEGTNVRQAVPSMPGVERVSVD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCCHHHHHHH
LLMGVAEQCMELGVPVLSLFPVIEPSLKTPDGREATNATGLIPRAVCELKKHFPELGVLT
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
DVALDPYTSHGQDGVLDEHGYVINDETVDILVEQAQTQAQAGVDIVAPSDMMDGRIGAIR
HHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEECCCHHHCCHHHHHH
ELLESEGHIHTRIMAYSAKFASAFYGPFRDAVGSAANLGKGNKMTYQLDPANSDEALREV
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEECCCCCHHHHHHH
RADIEEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKV
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHHH
MMESLLAFKRAGADGVLTYFALDAARLLRAQK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]