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The map label for this gene is pilQ [H]

Identifier: 186477543

GI number: 186477543

Start: 3147158

End: 3148729

Strand: Reverse

Name: pilQ [H]

Synonym: Bphy_2795

Alternate gene names: 186477543

Gene position: 3148729-3147158 (Counterclockwise)

Preceding gene: 186477544

Following gene: 186477542

Centisome position: 90.5

GC content: 66.41

Gene sequence:

>1572_bases
ATGACTTGGATCGCCTTCCTGCGATGGACACTTGCATGTGCTGCCTTTGTCGCAATGACGGCGCGTGCGGCCTTGCCTCC
GCTTCCCGCCGAGATGCCGTTCGACGATGCGATGACGCCGTCCGGCATGCCACCGCTGCCCCGCGTGATCACCACGGACG
TTGCCAATCCGTTCACGCCAGACGCGAGCGAGGACGGAGCGGCCCCGGAAGCGTCGCAGGCGCAGGCTTCCGCACGCGAC
GACCACGCGCCGGAATCGCGAACCGAACGGCGGACGGACGCACGGCAGGAACCGCTCGAAGGTCCGCCCGTTCTGCTCGC
GCCTGCCGCGCGTCTGAGCGAGAGTGCGTCGGCATCCGTTCCCGCCGACCGGCCCATCACGCTGCATTTCCAGCGCGGCG
AACTCGGCGCGGTGCTCGGCGCGTTCGCAAAGTTCACCGGCCTGAATATCGTCGCGAGCGACAAGGCGCGCGGCGCGGTC
ACGCTGCACCTGAACAGCGTGCCGTGGCGCACCGCATTCGATACGCTGCTCGATGTCAACGGCCTCGCGATGGAACAGCG
CGGCAACGTGATCTGGGTCGCACCGCAGTCGGAACTGGCCGCCCGCGAGCGTCAGCGTTTCGAGACGCATGCCCGCGCCG
CCGAGCTGGAACCGCTCGCGAGCCGCACCTTTGAGTTGCACTACGCCCATGCCGAGGAACTGCGCAAGCTGCTGACGGCG
TCGGGCAATCAGCGGGTGTTGTCCAAACGCGGCGCGGCGATGGCGGATCCGCGCACCAACCTGCTGTTCGTCACCGATCT
GGCTGCGCGGCTCGCGCAGATCGCCGAACTGGTCGCGTCGCTCGACCGGCCGACGCGCCAGGTGCTGATCGAGGCGCGCA
TCGTCGAAGCGGAAAAGGGCTTTTCTCGCAATCTCGGCGTGAAGCTGTCCATGATTGGCACCAACGAAGACGGCAAGGCG
ACGGGTCTGGTCGGCGGCAAGGACGGCGCGATCCTCGACCTGTCGGCGCGGCCTGTTTCCGGCTTCGACGCCGCAACGAT
GGGCCTGACTTTATTTGCCGCGCAGGCGACGCGGCTCGTGAACATCGAGCTCAGCGCGCTGGAGGCCGAGGGGCGCGGCC
AGATCGTATCGAGCCCGCGCGTCGTGACAGCGGACCGGATGAAGGCGGTCGTCGAGCAAGGCACCGAGCTGCCGTACCAG
GCAAAAGTCGGGCAGGGCGTGTCGGGCGTGCAGTTTCGCCGTGCGACCCTCAAACTGGAGGTCGAGCCGCAGATCACGCC
CGACGGCCGGGTGGTGCTCGATCTCGACGTGGCGAAGGACAGTGTCGGCGAGCAGACGGCGTCCGGACCTGCCATCAATA
CCAAGCACGTACAGACGCGCGTCGAAGTCGAAGATGGCGGAACGGTGTCGATCGGCGGAATCTACGAGACGGACGGCCGT
GATGATGTGACGCGCGTGCCACTCCTGGGCAAAATACCGGTTTTGGGCGTCTTTTTCCGTCATCGCGCCCATCGCGATCT
GCGCAGCGAACTCGTCGTTTTCATTACGCCGAGGGTGGTGCAGATAAATTGA

Upstream 100 bases:

>100_bases
AGTGGGGCGACGAACGAGTCGCCAATATCGACGCCCGCGCGCTGACGCTGACGAAACGCGACGGCAGCGCGCATTCGCTG
ACATTGGCGGAGGCCGTCGA

Downstream 100 bases:

>100_bases
CCAATCGCCGGCAAAGCGCCACGGGCGCGGGCCGAAAGCACCACCTGCGGCAGGCGCGCCGGCCGTCAGGCTCGACAAGT
CGGGCGCTTTGCCAGTAAGC

Product: type IV pilus secretin PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTWIAFLRWTLACAAFVAMTARAALPPLPAEMPFDDAMTPSGMPPLPRVITTDVANPFTPDASEDGAAPEASQAQASARD
DHAPESRTERRTDARQEPLEGPPVLLAPAARLSESASASVPADRPITLHFQRGELGAVLGAFAKFTGLNIVASDKARGAV
TLHLNSVPWRTAFDTLLDVNGLAMEQRGNVIWVAPQSELAARERQRFETHARAAELEPLASRTFELHYAHAEELRKLLTA
SGNQRVLSKRGAAMADPRTNLLFVTDLAARLAQIAELVASLDRPTRQVLIEARIVEAEKGFSRNLGVKLSMIGTNEDGKA
TGLVGGKDGAILDLSARPVSGFDAATMGLTLFAAQATRLVNIELSALEAEGRGQIVSSPRVVTADRMKAVVEQGTELPYQ
AKVGQGVSGVQFRRATLKLEVEPQITPDGRVVLDLDVAKDSVGEQTASGPAINTKHVQTRVEVEDGGTVSIGGIYETDGR
DDVTRVPLLGKIPVLGVFFRHRAHRDLRSELVVFITPRVVQIN

Sequences:

>Translated_523_residues
MTWIAFLRWTLACAAFVAMTARAALPPLPAEMPFDDAMTPSGMPPLPRVITTDVANPFTPDASEDGAAPEASQAQASARD
DHAPESRTERRTDARQEPLEGPPVLLAPAARLSESASASVPADRPITLHFQRGELGAVLGAFAKFTGLNIVASDKARGAV
TLHLNSVPWRTAFDTLLDVNGLAMEQRGNVIWVAPQSELAARERQRFETHARAAELEPLASRTFELHYAHAEELRKLLTA
SGNQRVLSKRGAAMADPRTNLLFVTDLAARLAQIAELVASLDRPTRQVLIEARIVEAEKGFSRNLGVKLSMIGTNEDGKA
TGLVGGKDGAILDLSARPVSGFDAATMGLTLFAAQATRLVNIELSALEAEGRGQIVSSPRVVTADRMKAVVEQGTELPYQ
AKVGQGVSGVQFRRATLKLEVEPQITPDGRVVLDLDVAKDSVGEQTASGPAINTKHVQTRVEVEDGGTVSIGGIYETDGR
DDVTRVPLLGKIPVLGVFFRHRAHRDLRSELVVFITPRVVQIN
>Mature_522_residues
TWIAFLRWTLACAAFVAMTARAALPPLPAEMPFDDAMTPSGMPPLPRVITTDVANPFTPDASEDGAAPEASQAQASARDD
HAPESRTERRTDARQEPLEGPPVLLAPAARLSESASASVPADRPITLHFQRGELGAVLGAFAKFTGLNIVASDKARGAVT
LHLNSVPWRTAFDTLLDVNGLAMEQRGNVIWVAPQSELAARERQRFETHARAAELEPLASRTFELHYAHAEELRKLLTAS
GNQRVLSKRGAAMADPRTNLLFVTDLAARLAQIAELVASLDRPTRQVLIEARIVEAEKGFSRNLGVKLSMIGTNEDGKAT
GLVGGKDGAILDLSARPVSGFDAATMGLTLFAAQATRLVNIELSALEAEGRGQIVSSPRVVTADRMKAVVEQGTELPYQA
KVGQGVSGVQFRRATLKLEVEPQITPDGRVVLDLDVAKDSVGEQTASGPAINTKHVQTRVEVEDGGTVSIGGIYETDGRD
DVTRVPLLGKIPVLGVFFRHRAHRDLRSELVVFITPRVVQIN

Specific function: Required for type IV pilus biogenesis and competence. Could function as a pore for exit of the pilus but also as a channel for entry of heme and antimicrobial agents and uptake of transforming DNA [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=414, Percent_Identity=35.9903381642512, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87082242, Length=352, Percent_Identity=28.9772727272727, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 56186; Mature: 56054

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWIAFLRWTLACAAFVAMTARAALPPLPAEMPFDDAMTPSGMPPLPRVITTDVANPFTP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
DASEDGAAPEASQAQASARDDHAPESRTERRTDARQEPLEGPPVLLAPAARLSESASASV
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHCCCCCCCC
PADRPITLHFQRGELGAVLGAFAKFTGLNIVASDKARGAVTLHLNSVPWRTAFDTLLDVN
CCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHCCC
GLAMEQRGNVIWVAPQSELAARERQRFETHARAAELEPLASRTFELHYAHAEELRKLLTA
CEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEHHHHHHHHHHHC
SGNQRVLSKRGAAMADPRTNLLFVTDLAARLAQIAELVASLDRPTRQVLIEARIVEAEKG
CCCHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHEEHHEEEECCCC
FSRNLGVKLSMIGTNEDGKATGLVGGKDGAILDLSARPVSGFDAATMGLTLFAAQATRLV
CCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHEEECCEEEE
NIELSALEAEGRGQIVSSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLKLE
EEEEEEEECCCCCCEECCCEEEEHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEEEEEE
VEPQITPDGRVVLDLDVAKDSVGEQTASGPAINTKHVQTRVEVEDGGTVSIGGIYETDGR
ECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCEEECCCC
DDVTRVPLLGKIPVLGVFFRHRAHRDLRSELVVFITPRVVQIN
CCCEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCEEEEEC
>Mature Secondary Structure 
TWIAFLRWTLACAAFVAMTARAALPPLPAEMPFDDAMTPSGMPPLPRVITTDVANPFTP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
DASEDGAAPEASQAQASARDDHAPESRTERRTDARQEPLEGPPVLLAPAARLSESASASV
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHCCCCCCCC
PADRPITLHFQRGELGAVLGAFAKFTGLNIVASDKARGAVTLHLNSVPWRTAFDTLLDVN
CCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHCCC
GLAMEQRGNVIWVAPQSELAARERQRFETHARAAELEPLASRTFELHYAHAEELRKLLTA
CEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEHHHHHHHHHHHC
SGNQRVLSKRGAAMADPRTNLLFVTDLAARLAQIAELVASLDRPTRQVLIEARIVEAEKG
CCCHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHEEHHEEEECCCC
FSRNLGVKLSMIGTNEDGKATGLVGGKDGAILDLSARPVSGFDAATMGLTLFAAQATRLV
CCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHEEECCEEEE
NIELSALEAEGRGQIVSSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLKLE
EEEEEEEECCCCCCEECCCEEEEHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEEEEEE
VEPQITPDGRVVLDLDVAKDSVGEQTASGPAINTKHVQTRVEVEDGGTVSIGGIYETDGR
ECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCEEECCCC
DDVTRVPLLGKIPVLGVFFRHRAHRDLRSELVVFITPRVVQIN
CCCEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]