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The map label for this gene is hisH [H]

Identifier: 186477503

GI number: 186477503

Start: 3106723

End: 3107364

Strand: Reverse

Name: hisH [H]

Synonym: Bphy_2755

Alternate gene names: 186477503

Gene position: 3107364-3106723 (Counterclockwise)

Preceding gene: 186477504

Following gene: 186477502

Centisome position: 89.31

GC content: 63.55

Gene sequence:

>642_bases
ATGAAAACTTCGATAGCGATTGTGGATTACGGAATGGGCAACCTGCGTTCGGTTGCCCAGGCGTTGAAGAAAGCCGCGCC
GGAAGCGGACGTGGCGATCGTCGACAAGCCCGAAGCGATCCGCGCGGCAGACCGTGTGGTGCTGCCCGGCCAGGGCGCGA
TGCCCGACTGCATGCGCTCGCTCGGCGAATCCGGTTTGCAGGAAGCGCTGATTGAAGCGTCGCGTAACAAGCCGCTGATG
GGCGTGTGCGTGGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCGACACGAACGGTCTCGGACTGCTGCCCGGCAA
GGTAGTGCGCTTCGATCTGGAAGGCCAGCTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAGATGGGCTGGAACCGTG
TCCGCCAGGCGCAGCCGCACCCGCTGTGGGACGGCGTCGCGGACAACGCGTTCTTCTACTTCGTGCACAGTTACTACGTC
GTGCCTGAAAACGCCGCGCATATGGCGGGCGAAACGGTGTATGGCGTGCCCTTTACCTCGGCGGTGGCGCGGGATAACAT
TTTCGCGACCCAGTTCCACCCGGAAAAGAGCGCCGACGCGGGCCTGCGCGTGTATCGCAACTTCGTACACTGGAATCCCT
GA

Upstream 100 bases:

>100_bases
CGGGAATCAATATCGGCACGCGGCTGATGGGTTTGATGTTGTCGGCGCTGGCGGTGGAGTTCATCGTCAACGGATTAAAG
GCGTTGCTGCCTAACTTGAA

Downstream 100 bases:

>100_bases
TCGCTGCCGTCAGTTCCGGCATTCATTTCTTTAACGGTTCATGTTTTCCTTCCCCGTAGCTTGCCCCGTTCCCATGCACA
CGCATGCGAAGACCGTCGGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALKKAAPEADVAIVDKPEAIRAADRVVLPGQGAMPDCMRSLGESGLQEALIEASRNKPLM
GVCVGEQMLFDWSAEGDTNGLGLLPGKVVRFDLEGQLQDDGSRFKVPQMGWNRVRQAQPHPLWDGVADNAFFYFVHSYYV
VPENAAHMAGETVYGVPFTSAVARDNIFATQFHPEKSADAGLRVYRNFVHWNP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAAPEADVAIVDKPEAIRAADRVVLPGQGAMPDCMRSLGESGLQEALIEASRNKPLM
GVCVGEQMLFDWSAEGDTNGLGLLPGKVVRFDLEGQLQDDGSRFKVPQMGWNRVRQAQPHPLWDGVADNAFFYFVHSYYV
VPENAAHMAGETVYGVPFTSAVARDNIFATQFHPEKSADAGLRVYRNFVHWNP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAAPEADVAIVDKPEAIRAADRVVLPGQGAMPDCMRSLGESGLQEALIEASRNKPLM
GVCVGEQMLFDWSAEGDTNGLGLLPGKVVRFDLEGQLQDDGSRFKVPQMGWNRVRQAQPHPLWDGVADNAFFYFVHSYYV
VPENAAHMAGETVYGVPFTSAVARDNIFATQFHPEKSADAGLRVYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=207, Percent_Identity=37.6811594202899, Blast_Score=121, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=33.7962962962963, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23353; Mature: 23353

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAAPEADVAIVDKPEAIRAADRVVLPGQGAMPDCMRS
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEALIEASRNKPLMGVCVGEQMLFDWSAEGDTNGLGLLPGKVVRFDLEGQLQDD
HHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCEECCCCEEEEEEECCCCCCC
GSRFKVPQMGWNRVRQAQPHPLWDGVADNAFFYFVHSYYVVPENAAHMAGETVYGVPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCHHHHCCCEEECCCCHH
AVARDNIFATQFHPEKSADAGLRVYRNFVHWNP
HHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAAPEADVAIVDKPEAIRAADRVVLPGQGAMPDCMRS
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEALIEASRNKPLMGVCVGEQMLFDWSAEGDTNGLGLLPGKVVRFDLEGQLQDD
HHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCEECCCCEEEEEEECCCCCCC
GSRFKVPQMGWNRVRQAQPHPLWDGVADNAFFYFVHSYYVVPENAAHMAGETVYGVPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCHHHHCCCEEECCCCHH
AVARDNIFATQFHPEKSADAGLRVYRNFVHWNP
HHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]