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The map label for this gene is hisA

Identifier: 186477502

GI number: 186477502

Start: 3105761

End: 3106513

Strand: Reverse

Name: hisA

Synonym: Bphy_2754

Alternate gene names: 186477502

Gene position: 3106513-3105761 (Counterclockwise)

Preceding gene: 186477503

Following gene: 186477501

Centisome position: 89.29

GC content: 63.48

Gene sequence:

>753_bases
ATGCTGCTGATCCCCGCCATCGACCTGAAAGACGGTCAGTGTGTACGCCTCAAACAAGGCGATATGGACCAGGCGACGAT
TTTCCACGAGGAACCGGCGGTGATGGCCCGACATTGGGTCGACCGTGGCGCGCGGCGCCTGCACCTCGTCGACCTGAATG
GCGCGTTTGCCGGAAAGCCGAAGAATGAAGACGCGATTCGCGCGATCATCGAAGAAGTCGGCGGCGAAATTCCCGTGCAA
CTGGGCGGCGGCATCCGCGATCTGAACACGATCGAACGCTATCTGGACGACGGTCTCGAGTACGTGATCATCGGCACGGC
GGCGGTGAAGAATCCGGGCTTCCTGCTCGAAGCGTGCACGGCTTTCGGCGGACACATCATCGTCGGCCTCGACGCGAAGG
ACGGCAAGGTGGCGACCGACGGCTGGAGCAAGCTGACGGGCCACGAAGTCGCCGATCTGGCGCGCAAGTTCGAGGACTAC
GGCTGCGAGTCGATCATCTACACGGACATCGGGCGCGACGGCATGCTTCAGGGCATCAATATCGAGGCGACGGTGCGGCT
CGCGCGCGCGGTGAAGATTCCCGTGATCGCGAGCGGCGGCCTGTCCAATCTCGGCGACATTGAAGCGCTGTGCGAAGTGG
AAGACGAAGGGATCGAAGGCGTGATCTGCGGACGCGCCATCTATTCGGGCGACCTGGACTTCGCCGCGGCGCAGACGCAC
GCGGATCGCCTGCGCGAATCGGACGACGCCTGA

Upstream 100 bases:

>100_bases
GAAAGACATGTACTAAACTAGCGGAACGGTCTGGCACGGGCTGTAGCCTGATTTCCCGCCAGCCGAACTTCAACTTTTTC
CCTGACAACACCCGATTGCT

Downstream 100 bases:

>100_bases
GCGCCTGTTCACCGGGCATCCGGCGCGGCTGACCTCGTGCCAGCGGCGCCAGGATGCGATGGGCAGCGCCCGGCGTGCCG
GCCTTGCGCCGTTCCCCTGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MLLIPAIDLKDGQCVRLKQGDMDQATIFHEEPAVMARHWVDRGARRLHLVDLNGAFAGKPKNEDAIRAIIEEVGGEIPVQ
LGGGIRDLNTIERYLDDGLEYVIIGTAAVKNPGFLLEACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKFEDY
GCESIIYTDIGRDGMLQGINIEATVRLARAVKIPVIASGGLSNLGDIEALCEVEDEGIEGVICGRAIYSGDLDFAAAQTH
ADRLRESDDA

Sequences:

>Translated_250_residues
MLLIPAIDLKDGQCVRLKQGDMDQATIFHEEPAVMARHWVDRGARRLHLVDLNGAFAGKPKNEDAIRAIIEEVGGEIPVQ
LGGGIRDLNTIERYLDDGLEYVIIGTAAVKNPGFLLEACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKFEDY
GCESIIYTDIGRDGMLQGINIEATVRLARAVKIPVIASGGLSNLGDIEALCEVEDEGIEGVICGRAIYSGDLDFAAAQTH
ADRLRESDDA
>Mature_250_residues
MLLIPAIDLKDGQCVRLKQGDMDQATIFHEEPAVMARHWVDRGARRLHLVDLNGAFAGKPKNEDAIRAIIEEVGGEIPVQ
LGGGIRDLNTIERYLDDGLEYVIIGTAAVKNPGFLLEACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKFEDY
GCESIIYTDIGRDGMLQGINIEATVRLARAVKIPVIASGGLSNLGDIEALCEVEDEGIEGVICGRAIYSGDLDFAAAQTH
ADRLRESDDA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=31.6872427983539, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788336, Length=213, Percent_Identity=24.4131455399061, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_BURP8 (B2JHY0)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858972.1
- ProteinModelPortal:   B2JHY0
- SMR:   B2JHY0
- GeneID:   6244237
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2754
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26868; Mature: 26868

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLIPAIDLKDGQCVRLKQGDMDQATIFHEEPAVMARHWVDRGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
KNEDAIRAIIEEVGGEIPVQLGGGIRDLNTIERYLDDGLEYVIIGTAAVKNPGFLLEACT
CCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHH
AFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKFEDYGCESIIYTDIGRDGMLQGIN
HCCCEEEEEEECCCCEEECCCCHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCC
IEATVRLARAVKIPVIASGGLSNLGDIEALCEVEDEGIEGVICGRAIYSGDLDFAAAQTH
HHHHEEEEHHEEEEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCEEECCCCCHHHHHHH
ADRLRESDDA
HHHHHCCCCC
>Mature Secondary Structure
MLLIPAIDLKDGQCVRLKQGDMDQATIFHEEPAVMARHWVDRGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
KNEDAIRAIIEEVGGEIPVQLGGGIRDLNTIERYLDDGLEYVIIGTAAVKNPGFLLEACT
CCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHH
AFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKFEDYGCESIIYTDIGRDGMLQGIN
HCCCEEEEEEECCCCEEECCCCHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCC
IEATVRLARAVKIPVIASGGLSNLGDIEALCEVEDEGIEGVICGRAIYSGDLDFAAAQTH
HHHHEEEEHHEEEEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCEEECCCCCHHHHHHH
ADRLRESDDA
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA