Click here to switch to the map view.

The map label for this gene is hisF [H]

Identifier: 186477501

GI number: 186477501

Start: 3104831

End: 3105604

Strand: Reverse

Name: hisF [H]

Synonym: Bphy_2753

Alternate gene names: 186477501

Gene position: 3105604-3104831 (Counterclockwise)

Preceding gene: 186477502

Following gene: 186477500

Centisome position: 89.26

GC content: 64.08

Gene sequence:

>774_bases
ATGGCTTTAGCTAAACGCATCATCCCCTGTCTGGACGTCACGGCTGGCCGCGTGGTGAAGGGCGTCAATTTCGTCGAGTT
GCGCGATGCGGGCGATCCCGTCGAAATCGCGCGCCGCTATGACGAGCAGGGCGCCGACGAACTCACGTTCCTCGACATCA
CGGCGACTTCCGACCAGCGCGACCTGATTCTGCCGATCATCGAAGCTGTCGCCTCGCAAGTGTTCATCCCGCTCACCGTC
GGCGGCGGGGTGCGTGCCGTCGAAGACGTCCGGCGTTTGCTGAATGCGGGCGCGGACAAGGTCAGCATGAACTCGTCGGC
GGTCGCGAATCCGCAACTTGTGCGAGATTCGACGGACAAATACGGCTCGCAATGTATCGTCGTCGCGATCGACGCAAAGC
GCGTATCCGCCGAAGGCGAGACGCCGCGCTGGGAAGTCTTCACGCACGGCGGGCGCAAGAACACGGGACTGGACGCTGTC
GAATGGGCGCGCAGGATGGCCGAACTCGGCGCGGGCGAAATCCTGCTGACCAGCATGGACCGCGACGGCACGAAGAGCGG
CTTTGACATCGCGCTGACGCGCGCCGTGTCGGACGCGGTGTCGATTCCTGTGATTGCCTCCGGCGGTGTCGGCTCGCTGC
AGCATCTTGCCGACGGCATCACGCAAGGCCGCGCGGACGCGGTGCTGGCCGCGAGCATCTTCCACTATGGCGAGCACACG
GTTGGCGAGTGCAAGCGCTTCATGGCCGATCAGGGCATTTCGGTGAGGTTGTAA

Upstream 100 bases:

>100_bases
CGATGGGCAGCGCCCGGCGTGCCGGCCTTGCGCCGTTCCCCTGCGAGTCACGCACGGGCGAGCGGCCTCACCAGCGGTAT
AGGCAAGAACTAGCAAGATC

Downstream 100 bases:

>100_bases
CGTGATGAATCCGTCTGAAGTCGGCTGGCTCGACAAGGTGCAGTGGGACGCGAACGGCCTCGTGCCCGTGATCGCGCAGG
AAGCGTCGACGAACGACGTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDSTDKYGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKNTGLDAV
EWARRMAELGAGEILLTSMDRDGTKSGFDIALTRAVSDAVSIPVIASGGVGSLQHLADGITQGRADAVLAASIFHYGEHT
VGECKRFMADQGISVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDSTDKYGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKNTGLDAV
EWARRMAELGAGEILLTSMDRDGTKSGFDIALTRAVSDAVSIPVIASGGVGSLQHLADGITQGRADAVLAASIFHYGEHT
VGECKRFMADQGISVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDSTDKYGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKNTGLDAVE
WARRMAELGAGEILLTSMDRDGTKSGFDIALTRAVSDAVSIPVIASGGVGSLQHLADGITQGRADAVLAASIFHYGEHTV
GECKRFMADQGISVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=41.6988416988417, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082028, Length=217, Percent_Identity=32.258064516129, Blast_Score=101, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=35.126582278481, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27375; Mature: 27244

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQR
CCCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDSTDK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCHH
YGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKNTGLDAVEWARRMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDIALTRAVSDAVSIPVIASGGVGSLQHLADGITQGRADAVLAASIFHYGEHT
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
VGECKRFMADQGISVRL
HHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQR
CCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDSTDK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCHH
YGSQCIVVAIDAKRVSAEGETPRWEVFTHGGRKNTGLDAVEWARRMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDIALTRAVSDAVSIPVIASGGVGSLQHLADGITQGRADAVLAASIFHYGEHT
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
VGECKRFMADQGISVRL
HHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA