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The map label for this gene is rocA [H]

Identifier: 186477437

GI number: 186477437

Start: 3041734

End: 3043425

Strand: Direct

Name: rocA [H]

Synonym: Bphy_2689

Alternate gene names: 186477437

Gene position: 3041734-3043425 (Clockwise)

Preceding gene: 186477435

Following gene: 186477438

Centisome position: 87.43

GC content: 61.52

Gene sequence:

>1692_bases
ATGACCCACTCACTCTTCACGAAGCACGAAGACACGCTGCAGAAAGCACTCGCCGCCATCGAAACCCGCGGCTACTGGAG
CCCGTTCTCCGAAATGCCGAGTCCCAAAGTGTACGGGGAAACCGCCAATGCGGACGGCGAGTCTGCATTCAAGGCTCATC
TGAATAAGACCTTCGAACTGGATCAGCCGAGCACGGGTGAGACGGTCGGCGCCGAGCAATCGCCGTTCGGCTTTCCGCTG
GGCATCCGTTACCCGAAATCGGATCCCGATGCGCTCATCGCCGCAGCATCCCACGCCCAGCGCCAATGGCGCGAAGCCGG
ACCCAAAGCCTGGGTAGGCGTAAGCCTTGAAGTCCTTGCGCGCATCAATCGCCTTAGTTTCGAGATCGGCTACAGCGTGA
TGCACACCACCGGCCAGGCATTCATGATGGCTTTCCAAGCGGGTGGTCCCCACGCCCAAGATCGCGCACTTGAAGCCGTC
GCCTATGCCTGGGATCAACTGCGCCGGATTCCGGCCGACGCCCATTGGGAAAAGCCGCAAGGCAAGAATCCTCCGCTCGC
GATGCGAAAGCACTTCACCATCGTGCCACGCGGCACGGGTCTCGTGCTCGGCTGCTGCACGTTTCCGACGTGGAATGGCT
ACCCCGGCCTGTTCGCAGACCTGGCAACGGGCAACACCGTGATTGTCAAACCGCACCCAGGCGCAATCCTGCCGCTTGCA
ATTACTGTCCGCGTCGCTCGCGAAGTTTTGCGCGAAGCCGGCTTCGATCCGAACGTCGTTACCTTGCTCGCGACGGATCC
AAACGACGGGGAACTCGTTCAGGCCCTTGCGCAACGCCCGGAGATCAAGTTGATCGATTTCACGGGCAGCACACAAAACG
GTACCTGGCTCGAACGGAATGCGCATCAGGCGCAAGTGTTCACCGAAAAGGCCGGCGTGAACCAGATCGTGATCGACTCG
GTCGACGACATCAAATCCGCCGCGCGCAACATCGCCTTCTCGCTCGCGCTGTACTCGGGTCAGATGTGCACGGCCCCGCA
AAACATCTATGTGCCGCGCGACGGCGTCCGCACCGCTGCAGGCCCGCTTTCGTTCGAAGCAGTTGCCGAAGCGATTGCGA
GCGCCGTCCAGAAGCTCGGTTCGGACCCGGCGCGCGCAGTCGAACTGCTCGGGGCGATCCAAAACGAAGGCGTCGCACGA
CGTATCGATGAAGCGCGCAAGCTCGGTCGCGTTCTGGCTGATAGTCAATCACTTCAGCACCCCGCGTTCGAAGGCGCCCG
CGTGCGCACGCCGCTGGTTATGCAACTCGATGCTGCAAAGGACCAGGCGAAGTTCACCAAGGAATGGTTCGGTCCCATCT
CGTTCGTGATAGCGACGGATTCGACCGAACAGTCGCTCGAGCTAGCCGGCCAGATCGCCTCCGAACACGGCGCGCTAACG
CTCTCCGTTTACAGCACCGACGAAAGCGTGCTCGAAGCGGCCACGGACGCTTCGATTCAAGGCGGCGTGGCACTTTCGAT
CAACCTGACTGGCGGCGTATTCGTCAATCAGTCGGCCGCGTTCTCGGACTTCCACGGGACGGGAGCCAACCCCGCTGCCA
ATGCCGCGCTCGCTGACGCCGCCTTCGTCGCGAACCGCTTCCGTGTCGTTCAGAGTCGAGTTCATGTTGAACCGAAGACG
GTTGCCGCATAA

Upstream 100 bases:

>100_bases
GCTCGCGCCGCATCCCAATAATGCTAACATTCACCAACCAACCGGTCGGTCAATAAATCGACGAGGTTCCTTCTAACGTT
CAGTCAGAAACCTGCCCGCC

Downstream 100 bases:

>100_bases
GCCGCCCGAACAATATAGGACGTTTGACCGACGTTTTGGCCTATAACTTCTGCGCGACTGAAAACGCGCCAGACGTCGAA
CTAAGATGCAGTTCAAGGGC

Product: phenylacetic acid degradation protein paaN

Products: NA

Alternate protein names: P5C dehydrogenase [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTHSLFTKHEDTLQKALAAIETRGYWSPFSEMPSPKVYGETANADGESAFKAHLNKTFELDQPSTGETVGAEQSPFGFPL
GIRYPKSDPDALIAAASHAQRQWREAGPKAWVGVSLEVLARINRLSFEIGYSVMHTTGQAFMMAFQAGGPHAQDRALEAV
AYAWDQLRRIPADAHWEKPQGKNPPLAMRKHFTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
ITVRVAREVLREAGFDPNVVTLLATDPNDGELVQALAQRPEIKLIDFTGSTQNGTWLERNAHQAQVFTEKAGVNQIVIDS
VDDIKSAARNIAFSLALYSGQMCTAPQNIYVPRDGVRTAAGPLSFEAVAEAIASAVQKLGSDPARAVELLGAIQNEGVAR
RIDEARKLGRVLADSQSLQHPAFEGARVRTPLVMQLDAAKDQAKFTKEWFGPISFVIATDSTEQSLELAGQIASEHGALT
LSVYSTDESVLEAATDASIQGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAAFVANRFRVVQSRVHVEPKT
VAA

Sequences:

>Translated_563_residues
MTHSLFTKHEDTLQKALAAIETRGYWSPFSEMPSPKVYGETANADGESAFKAHLNKTFELDQPSTGETVGAEQSPFGFPL
GIRYPKSDPDALIAAASHAQRQWREAGPKAWVGVSLEVLARINRLSFEIGYSVMHTTGQAFMMAFQAGGPHAQDRALEAV
AYAWDQLRRIPADAHWEKPQGKNPPLAMRKHFTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
ITVRVAREVLREAGFDPNVVTLLATDPNDGELVQALAQRPEIKLIDFTGSTQNGTWLERNAHQAQVFTEKAGVNQIVIDS
VDDIKSAARNIAFSLALYSGQMCTAPQNIYVPRDGVRTAAGPLSFEAVAEAIASAVQKLGSDPARAVELLGAIQNEGVAR
RIDEARKLGRVLADSQSLQHPAFEGARVRTPLVMQLDAAKDQAKFTKEWFGPISFVIATDSTEQSLELAGQIASEHGALT
LSVYSTDESVLEAATDASIQGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAAFVANRFRVVQSRVHVEPKT
VAA
>Mature_562_residues
THSLFTKHEDTLQKALAAIETRGYWSPFSEMPSPKVYGETANADGESAFKAHLNKTFELDQPSTGETVGAEQSPFGFPLG
IRYPKSDPDALIAAASHAQRQWREAGPKAWVGVSLEVLARINRLSFEIGYSVMHTTGQAFMMAFQAGGPHAQDRALEAVA
YAWDQLRRIPADAHWEKPQGKNPPLAMRKHFTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLAI
TVRVAREVLREAGFDPNVVTLLATDPNDGELVQALAQRPEIKLIDFTGSTQNGTWLERNAHQAQVFTEKAGVNQIVIDSV
DDIKSAARNIAFSLALYSGQMCTAPQNIYVPRDGVRTAAGPLSFEAVAEAIASAVQKLGSDPARAVELLGAIQNEGVARR
IDEARKLGRVLADSQSLQHPAFEGARVRTPLVMQLDAAKDQAKFTKEWFGPISFVIATDSTEQSLELAGQIASEHGALTL
SVYSTDESVLEAATDASIQGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAAFVANRFRVVQSRVHVEPKTV
AA

Specific function: Oxidizes Proline To Glutamate For Use As A Carbon And Nitrogen Source And Also Function As A Transcriptional Repressor Of The Put Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldehyde dehydrogenase family. RocA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787250, Length=372, Percent_Identity=27.1505376344086, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787684, Length=423, Percent_Identity=23.6406619385343, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081896, Length=291, Percent_Identity=28.1786941580756, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320917, Length=325, Percent_Identity=28.3076923076923, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016160
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR005932 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.5.1.12 [H]

Molecular weight: Translated: 60343; Mature: 60211

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSLFTKHEDTLQKALAAIETRGYWSPFSEMPSPKVYGETANADGESAFKAHLNKTFEL
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEECCCCCCCCHHHHHHHCCCEEEC
DQPSTGETVGAEQSPFGFPLGIRYPKSDPDALIAAASHAQRQWREAGPKAWVGVSLEVLA
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
RINRLSFEIGYSVMHTTGQAFMMAFQAGGPHAQDRALEAVAYAWDQLRRIPADAHWEKPQ
HHHHHHHHHCHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GKNPPLAMRKHFTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
CCCCCEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEECCCCCCEEHHH
ITVRVAREVLREAGFDPNVVTLLATDPNDGELVQALAQRPEIKLIDFTGSTQNGTWLERN
HHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEECC
AHQAQVFTEKAGVNQIVIDSVDDIKSAARNIAFSLALYSGQMCTAPQNIYVPRDGVRTAA
CCHHHHHHHHCCCCEEEECCHHHHHHHHHHHEEEEEEECCCEECCCCCEECCCCCCCCCC
GPLSFEAVAEAIASAVQKLGSDPARAVELLGAIQNEGVARRIDEARKLGRVLADSQSLQH
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCC
PAFEGARVRTPLVMQLDAAKDQAKFTKEWFGPISFVIATDSTEQSLELAGQIASEHGALT
CCCCCCEECCCEEEEECCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCEEE
LSVYSTDESVLEAATDASIQGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADA
EEEECCCHHHHHHHCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
AFVANRFRVVQSRVHVEPKTVAA
HHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
THSLFTKHEDTLQKALAAIETRGYWSPFSEMPSPKVYGETANADGESAFKAHLNKTFEL
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEECCCCCCCCHHHHHHHCCCEEEC
DQPSTGETVGAEQSPFGFPLGIRYPKSDPDALIAAASHAQRQWREAGPKAWVGVSLEVLA
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
RINRLSFEIGYSVMHTTGQAFMMAFQAGGPHAQDRALEAVAYAWDQLRRIPADAHWEKPQ
HHHHHHHHHCHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GKNPPLAMRKHFTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
CCCCCEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEECCCCCCEEHHH
ITVRVAREVLREAGFDPNVVTLLATDPNDGELVQALAQRPEIKLIDFTGSTQNGTWLERN
HHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEECC
AHQAQVFTEKAGVNQIVIDSVDDIKSAARNIAFSLALYSGQMCTAPQNIYVPRDGVRTAA
CCHHHHHHHHCCCCEEEECCHHHHHHHHHHHEEEEEEECCCEECCCCCEECCCCCCCCCC
GPLSFEAVAEAIASAVQKLGSDPARAVELLGAIQNEGVARRIDEARKLGRVLADSQSLQH
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCC
PAFEGARVRTPLVMQLDAAKDQAKFTKEWFGPISFVIATDSTEQSLELAGQIASEHGALT
CCCCCCEECCCEEEEECCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCEEE
LSVYSTDESVLEAATDASIQGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADA
EEEECCCHHHHHHHCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
AFVANRFRVVQSRVHVEPKTVAA
HHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA