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The map label for this gene is hipO [H]

Identifier: 186477435

GI number: 186477435

Start: 3033648

End: 3034841

Strand: Direct

Name: hipO [H]

Synonym: Bphy_2687

Alternate gene names: 186477435

Gene position: 3033648-3034841 (Clockwise)

Preceding gene: 186477432

Following gene: 186477437

Centisome position: 87.19

GC content: 58.04

Gene sequence:

>1194_bases
ATGAAACTACTCCCTGAAATCCAAGCCGCCCGCGGTGAAATTCAGACGCTCCGACGAACGATCCACGCCAATCCGGAATT
GAGGTATGAGGAGACGCAGACAGCGTCTTTGGTCGCCAAAACGCTGGCCGGATGGGGTATCGAAGTGCATGAGGGAATCG
GTAAAACCGGCGTGGTCGGCGTGCTCAAGCGCGGCGCAGGACCGAAATCGATCGGCTTGCGCGCCGACATGGACGCGCTG
CCTATCCAGGAATTGAATACTTTCGACCATCGGTCGAAAAACGAAGGGAAGATGCACGCGTGCGGCCACGACGGGCATAC
TGCGATGCTACTCGGCGCTGCGCGACATCTTGCAAAACACGGCGATTTCGACGGTACGATCGTGTTCATCTTCCAGCCCG
CCGAGGAAGGCGGAGCCGGCGCGCAAGCTATGATCGACGATGGCTTGTTCACGCGCTTTCCGGTCGATGCGGTGTTCGGC
ATTCACAACTGGCCGGGCATGCCGGCTGGGCATTTCGGTGTTACCGAAGGCCCGATCATGGCATCCAGTAACGAATTTCG
CATCCAAATCAAAGGCGTGGGGGCGCATGCGGCGTTGCCCCACAATGGACGCGATCCGGTTTTCACTGCTGTGCAGATCG
CAAATGGGTTACAAAGCGTGATCACGCGCAGCAAGAAGCCGCTCGATACAGCTGTCCTTTCGATTACGCAAATTCATGCC
GGCGATGCCGTGAACGTCGTTCCGGATCAAGCATGGCTCGCGGGTACAGTGCGAACTTTCACGACGGAGACGCTCGATCT
GATTGAGTCACGGATGCGCAAGATTGTGCAAAGCACCGCCGACGCGTATGAGTGTTCCGTTGAGATGACGTTTCACCGTA
ACTATCCGCCGACAATCAATAGCAGCAACGAGACACAGTTTGCCGCAAGAGTGATGCGGGAAGTCGTTGGAGACGAGAAG
GTCGACGCTTCCGTCGAACCGACGATGGGCGCCGAGGACTTTTCCTTCATGCTGCTGGCAAAGCCCGGGTGTTATGCGTT
TCTGGGCAACGGCGAAGGTGGTCACCGCGAGGCGGGACACGGTGCGGGCCCCTGCATGCTGCACAACGCGAGCTACGATT
TCAACGATGAACTGCTGCCGGTGGGTGCGACATACTGGGTTCGGTTGGCCGAGAGATTTTTGGCCGCAGGGTAA

Upstream 100 bases:

>100_bases
ATGGCCGTCAAAAATGGGGATCGGCGTAGAATCGATTTCGACTATCTCCCGGAATCGACTGTCCGGATCACACTGCTCAC
AGCCAGACACTCATTTCATC

Downstream 100 bases:

>100_bases
GAGCCGAACATTTAGCGCCGCGCGGAGTTGGCGACCGACTGCAAGACTTAAGGCAGAATCGTTATCGTCATCTCGCGCCT
CGGATCCCGCTTTAAGTTGT

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKLLPEIQAARGEIQTLRRTIHANPELRYEETQTASLVAKTLAGWGIEVHEGIGKTGVVGVLKRGAGPKSIGLRADMDAL
PIQELNTFDHRSKNEGKMHACGHDGHTAMLLGAARHLAKHGDFDGTIVFIFQPAEEGGAGAQAMIDDGLFTRFPVDAVFG
IHNWPGMPAGHFGVTEGPIMASSNEFRIQIKGVGAHAALPHNGRDPVFTAVQIANGLQSVITRSKKPLDTAVLSITQIHA
GDAVNVVPDQAWLAGTVRTFTTETLDLIESRMRKIVQSTADAYECSVEMTFHRNYPPTINSSNETQFAARVMREVVGDEK
VDASVEPTMGAEDFSFMLLAKPGCYAFLGNGEGGHREAGHGAGPCMLHNASYDFNDELLPVGATYWVRLAERFLAAG

Sequences:

>Translated_397_residues
MKLLPEIQAARGEIQTLRRTIHANPELRYEETQTASLVAKTLAGWGIEVHEGIGKTGVVGVLKRGAGPKSIGLRADMDAL
PIQELNTFDHRSKNEGKMHACGHDGHTAMLLGAARHLAKHGDFDGTIVFIFQPAEEGGAGAQAMIDDGLFTRFPVDAVFG
IHNWPGMPAGHFGVTEGPIMASSNEFRIQIKGVGAHAALPHNGRDPVFTAVQIANGLQSVITRSKKPLDTAVLSITQIHA
GDAVNVVPDQAWLAGTVRTFTTETLDLIESRMRKIVQSTADAYECSVEMTFHRNYPPTINSSNETQFAARVMREVVGDEK
VDASVEPTMGAEDFSFMLLAKPGCYAFLGNGEGGHREAGHGAGPCMLHNASYDFNDELLPVGATYWVRLAERFLAAG
>Mature_397_residues
MKLLPEIQAARGEIQTLRRTIHANPELRYEETQTASLVAKTLAGWGIEVHEGIGKTGVVGVLKRGAGPKSIGLRADMDAL
PIQELNTFDHRSKNEGKMHACGHDGHTAMLLGAARHLAKHGDFDGTIVFIFQPAEEGGAGAQAMIDDGLFTRFPVDAVFG
IHNWPGMPAGHFGVTEGPIMASSNEFRIQIKGVGAHAALPHNGRDPVFTAVQIANGLQSVITRSKKPLDTAVLSITQIHA
GDAVNVVPDQAWLAGTVRTFTTETLDLIESRMRKIVQSTADAYECSVEMTFHRNYPPTINSSNETQFAARVMREVVGDEK
VDASVEPTMGAEDFSFMLLAKPGCYAFLGNGEGGHREAGHGAGPCMLHNASYDFNDELLPVGATYWVRLAERFLAAG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=369, Percent_Identity=31.1653116531165, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787598, Length=307, Percent_Identity=29.3159609120521, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42762; Mature: 42762

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLPEIQAARGEIQTLRRTIHANPELRYEETQTASLVAKTLAGWGIEVHEGIGKTGVVG
CCCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCEEECCCCCCCHHHH
VLKRGAGPKSIGLRADMDALPIQELNTFDHRSKNEGKMHACGHDGHTAMLLGAARHLAKH
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHC
GDFDGTIVFIFQPAEEGGAGAQAMIDDGLFTRFPVDAVFGIHNWPGMPAGHFGVTEGPIM
CCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEE
ASSNEFRIQIKGVGAHAALPHNGRDPVFTAVQIANGLQSVITRSKKPLDTAVLSITQIHA
ECCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECC
GDAVNVVPDQAWLAGTVRTFTTETLDLIESRMRKIVQSTADAYECSVEMTFHRNYPPTIN
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
SSNETQFAARVMREVVGDEKVDASVEPTMGAEDFSFMLLAKPGCYAFLGNGEGGHREAGH
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
GAGPCMLHNASYDFNDELLPVGATYWVRLAERFLAAG
CCCCEEEECCCCCCCCCEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLLPEIQAARGEIQTLRRTIHANPELRYEETQTASLVAKTLAGWGIEVHEGIGKTGVVG
CCCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCEEECCCCCCCHHHH
VLKRGAGPKSIGLRADMDALPIQELNTFDHRSKNEGKMHACGHDGHTAMLLGAARHLAKH
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHC
GDFDGTIVFIFQPAEEGGAGAQAMIDDGLFTRFPVDAVFGIHNWPGMPAGHFGVTEGPIM
CCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEE
ASSNEFRIQIKGVGAHAALPHNGRDPVFTAVQIANGLQSVITRSKKPLDTAVLSITQIHA
ECCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECC
GDAVNVVPDQAWLAGTVRTFTTETLDLIESRMRKIVQSTADAYECSVEMTFHRNYPPTIN
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
SSNETQFAARVMREVVGDEKVDASVEPTMGAEDFSFMLLAKPGCYAFLGNGEGGHREAGH
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
GAGPCMLHNASYDFNDELLPVGATYWVRLAERFLAAG
CCCCEEEECCCCCCCCCEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]