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The map label for this gene is murF [H]

Identifier: 186477425

GI number: 186477425

Start: 3020255

End: 3021667

Strand: Reverse

Name: murF [H]

Synonym: Bphy_2677

Alternate gene names: 186477425

Gene position: 3021667-3020255 (Counterclockwise)

Preceding gene: 186477426

Following gene: 186477424

Centisome position: 86.85

GC content: 64.76

Gene sequence:

>1413_bases
ATGATGTTCACACTACGTGAAGCCGCCGCGGTGATTCCGGGCGCAGCGGTAAGTGGCGACGATTCGATCGCATTCGAACG
CGTGTCGACGGATAGCCGCACGTGCGGTCCCGGCGATCTGTTCGTCGCGCTCAAAGGCGACCGCTTCGACGCGCACGACT
TTCTGTCCGACGTCGCCGCGCACAACGTTAGCGCCGTGCTTGCGACGCGCACGCCCGACAACTTCGAGGTGCCCGCGCTG
CGCGTCACGGGCGACACGCGCACGGCCCTGGGCGCATTGGCGAATGGCTGGCGGCAGCGCTTTTCCTTGCCGCTGGTGGC
CGTGACGGGCAGCAACGGCAAGACCACGGTCAAGGAAATGATCTCGTCGATCTTCGCGGCGGCTGTCGGCGAAGCGTCGC
GTCTCGCGACGGCGGGCAATTTCAACAACGACGTCGGCCTGCCGCTGACGCTGTTTCGGCTGAACGAAACGCACAAGCTT
GCTGTTGTCGAGCTCGGCATGAACCATCCGGGCGAAACTGCGCTGCTCGGCAAAATTGCCGAGCCGACTGTCGCCGTCGT
CAACAACGCGCAGCGTGAGCATCAGGAATTCATGGCGACGGTGGAAGCCGTCGCGCTCGAACATGCAGCCGTGATTCACG
CGCTCAAGCCTGAAGGCGTCGCCGTGTTTCCGGCCAACGACGCGTACGCGGGTATCTGGCGCGTCGCCGCGACAGGCAAC
CGCATTATCGATTTCGCGCTGAATACGGCTGAACACACGACGGAAGCTGCGGTCAAAGGCTCGCTCGAAGGTAGCGAATT
GAGCATCGACACCGCGGAAGGCCACGTCGACGTCACGTTGCAAGTGCTTGGCGCACACAACGCGCACAACGCATTGGCAG
CGACTGCAGCCGCGCTTGCAGCGGGCGTGTCGCTCGACGCGATCAAGCGCGGTCTCGAAGCATTCGGCGCAGTCAAAGGC
CGCTTGCAGGTGAAGCGCGCGGTGCTCGGCGCGATGGCGGGCGCGACCGTGATCGACGACACGTACAACGCGAATCCCGA
CTCGATGCTCGCCGCGATCGACGTGCTCGCCGCGCGTCCTTCGCCGCGTGTGCTGGTGATGGGCGACATGGGTGAAGTCG
GCGACAACGGCCCCGAGTTTCATCGCGAAGTCGGCGCGTATGCAAAGGCGCAAGGCATCGATGCGCTGTACGCGCTCGGC
GAGGCGTCGCGCGATGCGTGCGCCGCATACGGCGACGAAGCGCACCACTGCGACGACGTGGAAGCACTCGTCGCGCACTT
GAAGCAAGCCGCTTACGGCGCGGCTGCAACGTATCTCGTGAAAGGCTCGCGCTTCATGAAAATGGAACGCGTGGTGGACG
CCGTTACGAGTCCACAACCCGCTGCACCGGGCACGGCGCCCGGCGCACACTGA

Upstream 100 bases:

>100_bases
CGACGCAGGAAATCATGGGCAAGAAGCGCGCCTTCTCGGATCAGGATCATGCACGGCTCGCACTCGCGGCCCGCGCCACG
CATGCACGCGGAGGCGGCGA

Downstream 100 bases:

>100_bases
AAGAGAAGGACAGGAGTATGTTACTGGCGCTGGCGCAATGGCTGCAGAACGACGTAGGCTTTTTGCGCGTGTTCAGTTAT
CTGACGTTTCGTGCTGTCGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAAHNVSAVLATRTPDNFEVPAL
RVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEMISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKL
AVVELGMNHPGETALLGKIAEPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN
RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALAAGVSLDAIKRGLEAFGAVKG
RLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARPSPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALG
EASRDACAAYGDEAHHCDDVEALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH

Sequences:

>Translated_470_residues
MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAAHNVSAVLATRTPDNFEVPAL
RVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEMISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKL
AVVELGMNHPGETALLGKIAEPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN
RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALAAGVSLDAIKRGLEAFGAVKG
RLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARPSPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALG
EASRDACAAYGDEAHHCDDVEALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH
>Mature_470_residues
MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAAHNVSAVLATRTPDNFEVPAL
RVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEMISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKL
AVVELGMNHPGETALLGKIAEPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN
RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALAAGVSLDAIKRGLEAFGAVKG
RLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARPSPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALG
EASRDACAAYGDEAHHCDDVEALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=457, Percent_Identity=40.0437636761488, Blast_Score=255, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48781; Mature: 48781

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAA
CEEEHHHHHHHCCCCEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
HNVSAVLATRTPDNFEVPALRVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEM
CCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
ISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKLAVVELGMNHPGETALLGKIA
HHHHHHHHHCCHHHHEECCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHCCC
EPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCEEEEEEECCC
RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALA
EEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHH
AGVSLDAIKRGLEAFGAVKGRLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC
SPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALGEASRDACAAYGDEAHHCDDV
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCCCCCCCH
EALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAA
CEEEHHHHHHHCCCCEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
HNVSAVLATRTPDNFEVPALRVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEM
CCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
ISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKLAVVELGMNHPGETALLGKIA
HHHHHHHHHCCHHHHEECCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHCCC
EPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCEEEEEEECCC
RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALA
EEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHH
AGVSLDAIKRGLEAFGAVKGRLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC
SPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALGEASRDACAAYGDEAHHCDDV
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCCCCCCCH
EALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]