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The map label for this gene is pilF [H]

Identifier: 186477403

GI number: 186477403

Start: 2995672

End: 2996970

Strand: Direct

Name: pilF [H]

Synonym: Bphy_2655

Alternate gene names: 186477403

Gene position: 2995672-2996970 (Clockwise)

Preceding gene: 186477402

Following gene: 186477404

Centisome position: 86.1

GC content: 65.82

Gene sequence:

>1299_bases
ATGATGAAGCCGTCATTTCTACAGGCGGCCCTCAGCCGGTATTCGATGCGCACCCCTTCCAACACTGCCACGCCTGCGCC
CAGCGTCGCCGCGAACACGCCGTCGCTTCAATCCGTCACGTCAGACGCCGACAGCCCGCCCGCCGTCCGCCTCCTGTCCG
GCACGCTGCAGGAAGCGGCGCGCCGCAATGCATCCGACCTGCACATCGAGCCGATGGAGCACGGCTGGCGAATGCGTCTG
CGTGTCGATGGTGTACTGCACGAACTGGCGCGCCCGCCCGTCCATCTACGTGACGCTTTCATCACGCGCGTGAAAGTGCT
GGCGCGCATGGATATCGCAGAACGGCGCGTGCCCCAGGACGGCCGCTTGCGCCTGCCCGTGACGGCGGGCCGCGTCGAAG
ACTACCGCGTGAATTCGCTGCCCACGCTGTTCGGGGAGAAGCTCGTGCTGCGGCGGCTCGAAGCGCTGCCCGCCGATCTG
TCGCTCGATTCGCTCGGACTCGCGCCCGGGCAGCGCGACATCGTGGACCGGTCGATCCGCTCGCCGCATGGGCTCGTGCT
CGTCACAGGTCCGACGGGCAGCGGCAAAACGATGTCGCTGTACTGCTTCCTGCAACTGTTGAACTGCGGTTCGCGCAATC
TCTGCTCGGTCGAAGATCCTGCCGAAATCCAGCTCGCGGGCGTCAATCAGGTCAGCGTGCGCGAGAAGGCGGGGCTGACG
TTTGCCGTCGCGCTTCGCGCGTTCCTTCGTCAGGATCCCGACGTCATCATGGTCGGTGAAATCCGCGACGAAGAAACGGC
CGACGTCGCCGTCAAAGCGGCTCAAACCGGGCATCTGGTGCTGTCGACGCTACACACGAACGACGCGCCGGCCGCCATCG
CGCGTCTGATCGACATCGGCGTCGAACCGTATAACCTCGCGGCGGCGCTGCGTCTCGTCACGGCGCAGCGGCTCGTGCGG
CGTCTGTGTTCGAGGTGCAAACAACATGCGGCAGAAACACACGCGTCGTTGCGCTCGGCTGGCGTGCCCGACGATCAGCT
CGCGCAGTGGCATCCCTACGCTCCATGCGGATGCGAAGCGTGTCATGGCATCGGCTTTCGCGGACGGGTCGGCATCCATC
AGGTCATGCCGATCTCCGATGCAATGCGCGAACTCATCGTCGCTCGCGCCGGTACACACGAGCTCGCTCGACAGGCGCAG
GCCGAACGCGTGCAGACATTGCGCGAAGCCGCGCTCGCCCGTGCTTTCGACGGCACGACGAGTCTGGCCGAAGCATCGAG
CGCAACGGAGGTTGCATGA

Upstream 100 bases:

>100_bases
TCGCCCTGGTCCGCCGGCGGGCTCGAAGATCAAGCGCCTCGTGCGCCGCTGACACTCGGTTGGCCAATCGGCCGAATAGT
CTGCACGCGCACGCTCCCGG

Downstream 100 bases:

>100_bases
CCCGCGCGTCGATCAGCAGCGAACAGCGCTTCGAGTGGCGCGCATTCGACACACATGGCGTACGCCGTCGCGGAACCGTG
ATCGCACCCGATCTCTCCGC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MMKPSFLQAALSRYSMRTPSNTATPAPSVAANTPSLQSVTSDADSPPAVRLLSGTLQEAARRNASDLHIEPMEHGWRMRL
RVDGVLHELARPPVHLRDAFITRVKVLARMDIAERRVPQDGRLRLPVTAGRVEDYRVNSLPTLFGEKLVLRRLEALPADL
SLDSLGLAPGQRDIVDRSIRSPHGLVLVTGPTGSGKTMSLYCFLQLLNCGSRNLCSVEDPAEIQLAGVNQVSVREKAGLT
FAVALRAFLRQDPDVIMVGEIRDEETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVEPYNLAAALRLVTAQRLVR
RLCSRCKQHAAETHASLRSAGVPDDQLAQWHPYAPCGCEACHGIGFRGRVGIHQVMPISDAMRELIVARAGTHELARQAQ
AERVQTLREAALARAFDGTTSLAEASSATEVA

Sequences:

>Translated_432_residues
MMKPSFLQAALSRYSMRTPSNTATPAPSVAANTPSLQSVTSDADSPPAVRLLSGTLQEAARRNASDLHIEPMEHGWRMRL
RVDGVLHELARPPVHLRDAFITRVKVLARMDIAERRVPQDGRLRLPVTAGRVEDYRVNSLPTLFGEKLVLRRLEALPADL
SLDSLGLAPGQRDIVDRSIRSPHGLVLVTGPTGSGKTMSLYCFLQLLNCGSRNLCSVEDPAEIQLAGVNQVSVREKAGLT
FAVALRAFLRQDPDVIMVGEIRDEETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVEPYNLAAALRLVTAQRLVR
RLCSRCKQHAAETHASLRSAGVPDDQLAQWHPYAPCGCEACHGIGFRGRVGIHQVMPISDAMRELIVARAGTHELARQAQ
AERVQTLREAALARAFDGTTSLAEASSATEVA
>Mature_432_residues
MMKPSFLQAALSRYSMRTPSNTATPAPSVAANTPSLQSVTSDADSPPAVRLLSGTLQEAARRNASDLHIEPMEHGWRMRL
RVDGVLHELARPPVHLRDAFITRVKVLARMDIAERRVPQDGRLRLPVTAGRVEDYRVNSLPTLFGEKLVLRRLEALPADL
SLDSLGLAPGQRDIVDRSIRSPHGLVLVTGPTGSGKTMSLYCFLQLLNCGSRNLCSVEDPAEIQLAGVNQVSVREKAGLT
FAVALRAFLRQDPDVIMVGEIRDEETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVEPYNLAAALRLVTAQRLVR
RLCSRCKQHAAETHASLRSAGVPDDQLAQWHPYAPCGCEACHGIGFRGRVGIHQVMPISDAMRELIVARAGTHELARQAQ
AERVQTLREAALARAFDGTTSLAEASSATEVA

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=408, Percent_Identity=42.6470588235294, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=46.5968586387435, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI87082188, Length=274, Percent_Identity=35.7664233576642, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 46791; Mature: 46791

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKPSFLQAALSRYSMRTPSNTATPAPSVAANTPSLQSVTSDADSPPAVRLLSGTLQEAA
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHH
RRNASDLHIEPMEHGWRMRLRVDGVLHELARPPVHLRDAFITRVKVLARMDIAERRVPQD
HCCCCCCEEEECCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GRLRLPVTAGRVEDYRVNSLPTLFGEKLVLRRLEALPADLSLDSLGLAPGQRDIVDRSIR
CCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
SPHGLVLVTGPTGSGKTMSLYCFLQLLNCGSRNLCSVEDPAEIQLAGVNQVSVREKAGLT
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCH
FAVALRAFLRQDPDVIMVGEIRDEETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIG
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHECCCCCCEEEEEECCCCHHHHHHHHHHCC
VEPYNLAAALRLVTAQRLVRRLCSRCKQHAAETHASLRSAGVPDDQLAQWHPYAPCGCEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHH
CHGIGFRGRVGIHQVMPISDAMRELIVARAGTHELARQAQAERVQTLREAALARAFDGTT
HHCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SLAEASSATEVA
HHHHCCCCCCCC
>Mature Secondary Structure
MMKPSFLQAALSRYSMRTPSNTATPAPSVAANTPSLQSVTSDADSPPAVRLLSGTLQEAA
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHH
RRNASDLHIEPMEHGWRMRLRVDGVLHELARPPVHLRDAFITRVKVLARMDIAERRVPQD
HCCCCCCEEEECCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GRLRLPVTAGRVEDYRVNSLPTLFGEKLVLRRLEALPADLSLDSLGLAPGQRDIVDRSIR
CCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
SPHGLVLVTGPTGSGKTMSLYCFLQLLNCGSRNLCSVEDPAEIQLAGVNQVSVREKAGLT
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCH
FAVALRAFLRQDPDVIMVGEIRDEETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIG
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHECCCCCCEEEEEECCCCHHHHHHHHHHCC
VEPYNLAAALRLVTAQRLVRRLCSRCKQHAAETHASLRSAGVPDDQLAQWHPYAPCGCEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHH
CHGIGFRGRVGIHQVMPISDAMRELIVARAGTHELARQAQAERVQTLREAALARAFDGTT
HHCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SLAEASSATEVA
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]