Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 186477401

GI number: 186477401

Start: 2993037

End: 2994029

Strand: Direct

Name: ispB [H]

Synonym: Bphy_2653

Alternate gene names: 186477401

Gene position: 2993037-2994029 (Clockwise)

Preceding gene: 186477396

Following gene: 186477402

Centisome position: 86.03

GC content: 62.24

Gene sequence:

>993_bases
ATGTCGTCAACCGCCACCCCAACCCCCAACGCAGCCAATCTGCTCGCTCCGATCGCCGAAGACATGCAGCAGGTGAATCG
CGTGATCCGGCACCGCCTGGCGTCGGAAGTGATGCTGATCAACCAGATCTCCGAGTACATCATCAGTGCCGGGGGCAAGC
GTCTGCGGCCCGCGCTGCTCCTGCTCGTGGCGGGGGCGCTCGGTGACCGGACGGGGTACCGGCACGAACTGGCGGCCGTC
GTCGAGTTCATCCATACGGCTACGCTGCTGCACGACGACGTCGTCGACGAATCCGACCTGCGTCGCGGCCGGCAGACCGC
TAACGCGCTGTTCGGCAACGCGGCCAGTGTGCTGGTCGGCGACTTCCTGTATTCGCGCTCCTTCCAGATGATGGTCGGCG
TTGGCAAGATGCGCGTGATGGAGATTCTTTCGGAAGCGACCAACATCATTTCCGAAGGCGAGGTGCTGCAACTGCTGAAC
ATGCATGACGCCGACGTCGACGAAGCGCGTTACATGCAGGTGATCCGCTACAAGACGGCCAAGCTGTTCGAGGCCGCCGC
CCAGCTGGGCGCGGTGCTGTCAGGAGCCGACGCGACCACGGAAGCCGCCGCGGCAGAGTTCGGCCGCCGCATCGGTACGG
CCTTCCAGATCATGGACGACTGGCTCGACTACACCGGCACGCCGGAATCGATGGGCAAGAACGCCGGCGACGATCTGCGC
GAAGGCAAGCCCACGCTGCCGCTTATCTATCTGATCGAACGAGGCACGCCGGAACAGTCGGCGCTCGCGCGCGAGGCTAT
CGAGCAAGGCGGCACCGATCGTTTCGACGAGATCTTTGAGGCGATCACGCGTTCCGGCGCGCTCGATCACACGCTTGAGT
GCGCAAAACAGGAAGCACAGGCCGCGGCAACAGCAATTTCTTCGTTTCCCAGTTCCATTTACAAAGAAAGCCTGTTAAAC
TTATGTTCTTACTCGACGACGAGGCAGTCTTAA

Upstream 100 bases:

>100_bases
CCACGCGGAAACGGACCGAAACCGCCATGACCCAATGGCATACGGCGCGGAAATACCGCAGTTCGCCTTATAATTCGCGG
CACTACCCGAATTCGCCACC

Downstream 100 bases:

>100_bases
ACAAGACTGAAACGCCGGATTAGTCTGAATCGAGTGACAGCAGTACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCT
ACGTTCGGGACGTAGAGGCC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSSTATPTPNAANLLAPIAEDMQQVNRVIRHRLASEVMLINQISEYIISAGGKRLRPALLLLVAGALGDRTGYRHELAAV
VEFIHTATLLHDDVVDESDLRRGRQTANALFGNAASVLVGDFLYSRSFQMMVGVGKMRVMEILSEATNIISEGEVLQLLN
MHDADVDEARYMQVIRYKTAKLFEAAAQLGAVLSGADATTEAAAAEFGRRIGTAFQIMDDWLDYTGTPESMGKNAGDDLR
EGKPTLPLIYLIERGTPEQSALAREAIEQGGTDRFDEIFEAITRSGALDHTLECAKQEAQAAATAISSFPSSIYKESLLN
LCSYSTTRQS

Sequences:

>Translated_330_residues
MSSTATPTPNAANLLAPIAEDMQQVNRVIRHRLASEVMLINQISEYIISAGGKRLRPALLLLVAGALGDRTGYRHELAAV
VEFIHTATLLHDDVVDESDLRRGRQTANALFGNAASVLVGDFLYSRSFQMMVGVGKMRVMEILSEATNIISEGEVLQLLN
MHDADVDEARYMQVIRYKTAKLFEAAAQLGAVLSGADATTEAAAAEFGRRIGTAFQIMDDWLDYTGTPESMGKNAGDDLR
EGKPTLPLIYLIERGTPEQSALAREAIEQGGTDRFDEIFEAITRSGALDHTLECAKQEAQAAATAISSFPSSIYKESLLN
LCSYSTTRQS
>Mature_329_residues
SSTATPTPNAANLLAPIAEDMQQVNRVIRHRLASEVMLINQISEYIISAGGKRLRPALLLLVAGALGDRTGYRHELAAVV
EFIHTATLLHDDVVDESDLRRGRQTANALFGNAASVLVGDFLYSRSFQMMVGVGKMRVMEILSEATNIISEGEVLQLLNM
HDADVDEARYMQVIRYKTAKLFEAAAQLGAVLSGADATTEAAAAEFGRRIGTAFQIMDDWLDYTGTPESMGKNAGDDLRE
GKPTLPLIYLIERGTPEQSALAREAIEQGGTDRFDEIFEAITRSGALDHTLECAKQEAQAAATAISSFPSSIYKESLLNL
CSYSTTRQS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=29.5238095238095, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1789578, Length=311, Percent_Identity=52.0900321543408, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI1786623, Length=235, Percent_Identity=32.3404255319149, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=302, Percent_Identity=31.4569536423841, Blast_Score=153, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319475, Length=242, Percent_Identity=31.8181818181818, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=236, Percent_Identity=24.5762711864407, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24651612, Length=287, Percent_Identity=30.3135888501742, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35878; Mature: 35747

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTATPTPNAANLLAPIAEDMQQVNRVIRHRLASEVMLINQISEYIISAGGKRLRPALL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLVAGALGDRTGYRHELAAVVEFIHTATLLHDDVVDESDLRRGRQTANALFGNAASVLVG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
DFLYSRSFQMMVGVGKMRVMEILSEATNIISEGEVLQLLNMHDADVDEARYMQVIRYKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KLFEAAAQLGAVLSGADATTEAAAAEFGRRIGTAFQIMDDWLDYTGTPESMGKNAGDDLR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHH
EGKPTLPLIYLIERGTPEQSALAREAIEQGGTDRFDEIFEAITRSGALDHTLECAKQEAQ
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAATAISSFPSSIYKESLLNLCSYSTTRQS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSTATPTPNAANLLAPIAEDMQQVNRVIRHRLASEVMLINQISEYIISAGGKRLRPALL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLVAGALGDRTGYRHELAAVVEFIHTATLLHDDVVDESDLRRGRQTANALFGNAASVLVG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
DFLYSRSFQMMVGVGKMRVMEILSEATNIISEGEVLQLLNMHDADVDEARYMQVIRYKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KLFEAAAQLGAVLSGADATTEAAAAEFGRRIGTAFQIMDDWLDYTGTPESMGKNAGDDLR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHH
EGKPTLPLIYLIERGTPEQSALAREAIEQGGTDRFDEIFEAITRSGALDHTLECAKQEAQ
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAATAISSFPSSIYKESLLNLCSYSTTRQS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]