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The map label for this gene is proS

Identifier: 186477394

GI number: 186477394

Start: 2986249

End: 2987985

Strand: Reverse

Name: proS

Synonym: Bphy_2646

Alternate gene names: 186477394

Gene position: 2987985-2986249 (Counterclockwise)

Preceding gene: 186477397

Following gene: 186477393

Centisome position: 85.88

GC content: 62.41

Gene sequence:

>1737_bases
ATGAAAGCCTCCCGTTTCTTTATTGGCACCCTGAAGGAAGCGCCCGCCGACGCGGAAATCGTCAGTCACAAGCTCATGGT
GCGTGCCGGGATGATCCGGCGCGTGGCTGGCGGCATCTACAACTACCTGCCAATTGGCCTGCGTTCGATCCGCAAGGTAG
AAGCGATCGTGCGCGAGGAAATGAACCGGGCAGGCGCGATCGAACTGCTGATGCCGGCTGTGCAGCCGGCCGAGCTGTGG
CAGGAATCGGGCCGCTGGGAAAAATATGGTCCCGAACTACTGCGCTTCAAGGATCGCAAGCAAAGCGACTTCGTGATCGG
GCCCACTCACGAAGAAGTGGTCACGGACATCGCCCGCGGCCAGATCAAGAGCTACCGCCAGTTGCCTGTGAACTTCTACC
AGGTACAGACCAAGTTCCGGGATGAAATCCGTCCGCGTTTCGGCGTAATGCGCGGCCGCGAATTCATCATGAAGGACGCG
TATTCGTTCGATAAGGACATGGACGGCCTGCGCGAGTCGTATCGCAAGATGTACGACGCGTACGTGCGCATTTTCACGCG
CCTTGGTCTCGACTTCCGCGCGGTCGCGGCGGACAACGGCTCGATCGGCGGCAGCGGCTCGCACGAATTCCACGTGATCG
CCGATACGGGCGAGGACGACATCGCGTATTGCCCGACGTCGGACTTCGCATCGAACGTCGAAGCGGCCGAAGCGCTGCCG
CTGATCGCCGAGCGCGCCGCGCCGAAGGAAGAGCTCAGGAAAACGTCGACGCCGGGCAAGGCAAAGTGCGAAGCCGTCGC
CGAGCACCTGAACATCCCGCTCGAAAAGACGATCAAGTCGATCATCCTTGCCACGGAAAACGAAGGCGCCGAGCCGACCA
TCTGGCTCCTGATGTTGCGCGGCGACCACGATCTGAACGAGATCAAGGTCAACAAGCTGCCGGGCCTCGGCGAATTCCGC
TTCGCGACGGAAGAGGAAATCGTCGAGTGGTTCGGCACGCCGCCGGGCTACCTCGGGCCGCTGAATACGAAGAAGCCGAT
CAAGGTGATCGCGGACCGCACAGTCGCGAACATGAGCGACTTCGTGGTCGGCACGAACGAAGTGGACTTCCATACTACCG
GCGTCAACTGGGGCCGCGATCTGCCCGAGCCCGTCGTCGCGGACATCCGCAACGTGAAGAAGGACGATCCGTCGCCGGAC
GGCAAGGGCGTGATCGACATCTGCCGGGGCATCGAAGTCGGCCATGTGTTTCAGCTCGGCACCAAGTATTCGGAAGCGAT
GAACGCGACCTTCCTCGACGAATCGGGCAAGCCGCAACCGATGCAGATGGGCTGTTACGGCATCGGCATCACGCGTATCC
TCGGCGCGGCCATCGAACAGAACTTCGACGACAAGGGCATCATCTGGCCGGAATCGATCGCGCCGTTCGAAGTCGTGCTG
TGCCCGATGGGCTACGACCGCAGCGACGCCGTGCGCGAGCAGGCGGACAAGCTGCACGACGAACTGACGGCGGCGGGCAT
CGACGTGATTCTGGACGATCGCGGCGAGCGTCCGGGCGTGATGTTCGCCGACTGGGAACTGATCGGCGTGCCGCATCGCC
TCGTGATCGGTGAACGTGGCCTGAAGGACGGCAAGATCGAGTATCAGGGCCGCCGCGATGCGGAAGCCACGCTGTTGCCC
GTCGAAGCGGCGGCACAGGCAGTGATCGACAAGATCCACGCAGCGCTCGCGCGCTGA

Upstream 100 bases:

>100_bases
TTCGACGTGCGCCGGCATGGCCAGGCGCTCAGGCGCTCAGACCCATCGTCTTACCCCTTTCGGGCGGTCCCTTTGGTCCG
CCGCTTTTGGAAAATTCTGA

Downstream 100 bases:

>100_bases
TGGGAGCGGACGGTGGAGTACAACTTCCTGTCCGCGACCATCCTGCTGATTCTCATCACTGATCCACTCGGCAACATCCC
GCTCTTCATCAATTGCCTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARGQIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDMDGLRESYRKMYDAYVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIADTGEDDIAYCPTSDFASNVEAAEALP
LIAERAAPKEELRKTSTPGKAKCEAVAEHLNIPLEKTIKSIILATENEGAEPTIWLLMLRGDHDLNEIKVNKLPGLGEFR
FATEEEIVEWFGTPPGYLGPLNTKKPIKVIADRTVANMSDFVVGTNEVDFHTTGVNWGRDLPEPVVADIRNVKKDDPSPD
GKGVIDICRGIEVGHVFQLGTKYSEAMNATFLDESGKPQPMQMGCYGIGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDAVREQADKLHDELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDAEATLLP
VEAAAQAVIDKIHAALAR

Sequences:

>Translated_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARGQIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDMDGLRESYRKMYDAYVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIADTGEDDIAYCPTSDFASNVEAAEALP
LIAERAAPKEELRKTSTPGKAKCEAVAEHLNIPLEKTIKSIILATENEGAEPTIWLLMLRGDHDLNEIKVNKLPGLGEFR
FATEEEIVEWFGTPPGYLGPLNTKKPIKVIADRTVANMSDFVVGTNEVDFHTTGVNWGRDLPEPVVADIRNVKKDDPSPD
GKGVIDICRGIEVGHVFQLGTKYSEAMNATFLDESGKPQPMQMGCYGIGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDAVREQADKLHDELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDAEATLLP
VEAAAQAVIDKIHAALAR
>Mature_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARGQIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDMDGLRESYRKMYDAYVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIADTGEDDIAYCPTSDFASNVEAAEALP
LIAERAAPKEELRKTSTPGKAKCEAVAEHLNIPLEKTIKSIILATENEGAEPTIWLLMLRGDHDLNEIKVNKLPGLGEFR
FATEEEIVEWFGTPPGYLGPLNTKKPIKVIADRTVANMSDFVVGTNEVDFHTTGVNWGRDLPEPVVADIRNVKKDDPSPD
GKGVIDICRGIEVGHVFQLGTKYSEAMNATFLDESGKPQPMQMGCYGIGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDAVREQADKLHDELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDAEATLLP
VEAAAQAVIDKIHAALAR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=227, Percent_Identity=44.4933920704846, Blast_Score=220, Evalue=2e-57,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=56.7190226876091, Blast_Score=687, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=260, Percent_Identity=36.9230769230769, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=82, Percent_Identity=41.4634146341463, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71991342, Length=176, Percent_Identity=27.8409090909091, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=221, Percent_Identity=39.3665158371041, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=37.9591836734694, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BURP8 (B2JHD3)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858864.1
- ProteinModelPortal:   B2JHD3
- SMR:   B2JHD3
- GeneID:   6244129
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2646
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64359; Mature: 64359

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARG
HHHCCCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDMDGLRESYRKMYDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIADTGEDDIAYCPTSDFASNVEAAEALP
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCHHHHCCCHHHHHHH
LIAERAAPKEELRKTSTPGKAKCEAVAEHLNIPLEKTIKSIILATENEGAEPTIWLLMLR
HHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKVNKLPGLGEFRFATEEEIVEWFGTPPGYLGPLNTKKPIKVIADRTVANMSD
CCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGTNEVDFHTTGVNWGRDLPEPVVADIRNVKKDDPSPDGKGVIDICRGIEVGHVFQLG
HEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHC
TKYSEAMNATFLDESGKPQPMQMGCYGIGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDAVREQADKLHDELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDAEATLLPVEAAAQAVIDKIHAALAR
CCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEKYGPELLRFKDRKQSDFVIGPTHEEVVTDIARG
HHHCCCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQLPVNFYQVQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDMDGLRESYRKMYDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIADTGEDDIAYCPTSDFASNVEAAEALP
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCHHHHCCCHHHHHHH
LIAERAAPKEELRKTSTPGKAKCEAVAEHLNIPLEKTIKSIILATENEGAEPTIWLLMLR
HHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKVNKLPGLGEFRFATEEEIVEWFGTPPGYLGPLNTKKPIKVIADRTVANMSD
CCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGTNEVDFHTTGVNWGRDLPEPVVADIRNVKKDDPSPDGKGVIDICRGIEVGHVFQLG
HEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHC
TKYSEAMNATFLDESGKPQPMQMGCYGIGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDAVREQADKLHDELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDAEATLLPVEAAAQAVIDKIHAALAR
CCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA