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The map label for this gene is yafJ [C]

Identifier: 186477316

GI number: 186477316

Start: 2888720

End: 2889625

Strand: Direct

Name: yafJ [C]

Synonym: Bphy_2568

Alternate gene names: 186477316

Gene position: 2888720-2889625 (Clockwise)

Preceding gene: 186477313

Following gene: 186477324

Centisome position: 83.03

GC content: 62.58

Gene sequence:

>906_bases
ATGTGCCAACTACTCGGAATGAATTGCGCCGCGCCGACGGACGTCACGTTCTCGTTCACCGGCTTCGCGGCGCGCGGCGG
CGTCACCGACCACCACTCCGACGGCTGGGGCATCGCCTTCTTCGAAGACAAGGCCTGTCGCCTGTTCATCGACCATCAGT
CGTCGGCGACCTCGCCGATAGCGGAGATGGTCAAGCGCTACCCGATCAAGTCAAAAAACACGATTGCCCATATCCGCAAG
GCGACGCAAGGGCATATCGTGCTCGAGAACTGCCACCCGTTCATGCGGGAGTTGTGGGGACGGCACTGGATCTTCGCGCA
CAACGGCGATCTGCAGAACTACGGCCCGGTGCTGTCGGGCGTCTATCAGCCCGTCGGCAGCACGGATAGCGAACTGGCCT
TCTGCGCGCTGCTGCAAGGTTTGCGCAAGGCGTTTCCGGCCTCGCAGCCGCCGCTCGACGAACTGTTCGCCGCGCTCGAA
ACGCTGACCAAGGAAATCACCCAGTTCGGCGTGTTCAATTTCCTGATGTCGAACGGCCAGGCGCTGTTCGCGCATTGCTC
GACGCATCTGCATTACCTCGTGCGCAGCTGGCCTTTTTCGACCGCACATCTGGTCGACGCGGACGTTTCGATCGACTTCG
CCAAATACACGACGCCCGAAGATCGCGTCGCCGTGATCGCCACGCAGCCGTTGACCGACAATGAAATCTGGACGGCCTTC
GAGCCCGGCGATCTGCTGATGTTCCAGCATGGCGAAGTGATCGCACGCGCCAATGTGCCTGTGCCGCCCGCCGTGCTGGA
GAAGGCGCGCAACCCCGCTTGCGACCCCAGCGCGTCGGCGAGCATGCTGCCCGCCGCTTCCGTCGTCGATCTCGAATCCG
ACGACGCGGCAGCGTATGAATCGTAA

Upstream 100 bases:

>100_bases
TTTGCCGTTTCTGGCTCGGTGATATGCTTTGAAAATCATCATTGTAACGGGTCGGCCCACGTGGGCTCATACCTCGTTAG
AAAGGCTTGTCTTCCGCATC

Downstream 100 bases:

>100_bases
TGGCCTTATCGGCTGACGGGCACGCACGTGCGCGTACGCCGCGATGCACGGCGCAGTGTCATGCTTCGCGCTCGTTCGCG
CCGGCGATATCAGTGCCGCG

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MCQLLGMNCAAPTDVTFSFTGFAARGGVTDHHSDGWGIAFFEDKACRLFIDHQSSATSPIAEMVKRYPIKSKNTIAHIRK
ATQGHIVLENCHPFMRELWGRHWIFAHNGDLQNYGPVLSGVYQPVGSTDSELAFCALLQGLRKAFPASQPPLDELFAALE
TLTKEITQFGVFNFLMSNGQALFAHCSTHLHYLVRSWPFSTAHLVDADVSIDFAKYTTPEDRVAVIATQPLTDNEIWTAF
EPGDLLMFQHGEVIARANVPVPPAVLEKARNPACDPSASASMLPAASVVDLESDDAAAYES

Sequences:

>Translated_301_residues
MCQLLGMNCAAPTDVTFSFTGFAARGGVTDHHSDGWGIAFFEDKACRLFIDHQSSATSPIAEMVKRYPIKSKNTIAHIRK
ATQGHIVLENCHPFMRELWGRHWIFAHNGDLQNYGPVLSGVYQPVGSTDSELAFCALLQGLRKAFPASQPPLDELFAALE
TLTKEITQFGVFNFLMSNGQALFAHCSTHLHYLVRSWPFSTAHLVDADVSIDFAKYTTPEDRVAVIATQPLTDNEIWTAF
EPGDLLMFQHGEVIARANVPVPPAVLEKARNPACDPSASASMLPAASVVDLESDDAAAYES
>Mature_301_residues
MCQLLGMNCAAPTDVTFSFTGFAARGGVTDHHSDGWGIAFFEDKACRLFIDHQSSATSPIAEMVKRYPIKSKNTIAHIRK
ATQGHIVLENCHPFMRELWGRHWIFAHNGDLQNYGPVLSGVYQPVGSTDSELAFCALLQGLRKAFPASQPPLDELFAALE
TLTKEITQFGVFNFLMSNGQALFAHCSTHLHYLVRSWPFSTAHLVDADVSIDFAKYTTPEDRVAVIATQPLTDNEIWTAF
EPGDLLMFQHGEVIARANVPVPPAVLEKARNPACDPSASASMLPAASVVDLESDDAAAYES

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=255, Percent_Identity=51.7647058823529, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 32969; Mature: 32969

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCAAPTDVTFSFTGFAARGGVTDHHSDGWGIAFFEDKACRLFIDHQSSATSPI
CCEEECCCCCCCCCCEEEECCEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHH
AEMVKRYPIKSKNTIAHIRKATQGHIVLENCHPFMRELWGRHWIFAHNGDLQNYGPVLSG
HHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHCCCEEEEEECCCCCHHHHHHHH
VYQPVGSTDSELAFCALLQGLRKAFPASQPPLDELFAALETLTKEITQFGVFNFLMSNGQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALFAHCSTHLHYLVRSWPFSTAHLVDADVSIDFAKYTTPEDRVAVIATQPLTDNEIWTAF
EEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEHCCCCCCCCEEEEEECCCCCCCCEEEE
EPGDLLMFQHGEVIARANVPVPPAVLEKARNPACDPSASASMLPAASVVDLESDDAAAYE
CCCCEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
S
C
>Mature Secondary Structure
MCQLLGMNCAAPTDVTFSFTGFAARGGVTDHHSDGWGIAFFEDKACRLFIDHQSSATSPI
CCEEECCCCCCCCCCEEEECCEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHH
AEMVKRYPIKSKNTIAHIRKATQGHIVLENCHPFMRELWGRHWIFAHNGDLQNYGPVLSG
HHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHCCCEEEEEECCCCCHHHHHHHH
VYQPVGSTDSELAFCALLQGLRKAFPASQPPLDELFAALETLTKEITQFGVFNFLMSNGQ
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALFAHCSTHLHYLVRSWPFSTAHLVDADVSIDFAKYTTPEDRVAVIATQPLTDNEIWTAF
EEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEHCCCCCCCCEEEEEECCCCCCCCEEEE
EPGDLLMFQHGEVIARANVPVPPAVLEKARNPACDPSASASMLPAASVVDLESDDAAAYE
CCCCEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]