| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is purB [H]
Identifier: 186477324
GI number: 186477324
Start: 2896525
End: 2897913
Strand: Direct
Name: purB [H]
Synonym: Bphy_2576
Alternate gene names: 186477324
Gene position: 2896525-2897913 (Clockwise)
Preceding gene: 186477316
Following gene: 186477329
Centisome position: 83.25
GC content: 61.41
Gene sequence:
>1389_bases ATGTCCGACACCCGCCCCGACACCCTCTTCGCGCTGACCGCCCTTTCCCCGCTCGACGGCCGTTACGCATCGAAAACCGA AGCTTTGCGCGACTGGCTCTCAGAGGCCGCGTTCATGCGCCATCGCGTGACGGTTGAAATTCACTGGCTGATCGCGCTGT CGCACGCAGGCTTCGCCGAAGTACCGCGCTTCTCGGCAGCGTCGGAGCAGTTCCTGCTGCAGCTCGCCGAGCGCTTCACC GCCCACGACGCCGCGCGCATCAAGGACATCGAGCGCGTGACGAACCACGACGTGAAGGCCGTCGAATACTGGCTGAAAGA ATCGGTGAAGGGTCAGCCGGAACTGGAGCGCGCGAGCGAATTCATCCACTTCGCGTGCACGTCCGAGGACATCAATAACA CGTCACACGGCCTGATGCTCGCCGGCGCGCGCGAACACGTGATCCTGCCCGCGCTGCGCGCCGTGCATCAGCGTCTCGTC GCGCTTGCGCATGCGCAGGCCGATCAGCCGATGCTGTCGCGCACGCACGGCCAGCCTGCCAGCCCGACCACACTGGGCAA GGAAATCGCGAACGTCGCGGCGCGGCTGTCGCGCGCGATCGATCGAATCGCGAAGGTCGAACTGCTCGGCAAGATGAACG GCGCCGTCGGCAACTTCAATGCGCATCTGTCGGCGTATCCGGAATTCGACTGGGAAGCGTTCTCGAAGGAAGTCGTCGAG CAGCGTCTGAAGCTCACGTTCAACCCGTACACGATCCAGATCGAGCCGCACGACTACATGGCCGAACTGTTCGACGCGAT CTCGCGCGCAAACACGATCCTGCTCGACCTCGATCGTGACGTGTGGGGTTATATCTCGCTTGGGTATTTCAAGCAGAAGA CGAAGGCGGGCGAAATCGGTTCGTCGACAATGCCGCACAAGGTCAATCCGATCGACTTCGAAAATTCGGAAGGCAATCTG GGCCTTGCGAATGCGACGCTGCGGCATCTGGCCGACAAGCTGCCCGTCTCGCGCTGGCAGCGCGACCTGACCGATTCGAC GGTGCTGCGCAATATCGGTGTTGCGTTCGGGTATTCGCTGTTGGCGTATGACGCGCTCAATCGCGGGCTCGACAAGCTCG AGGTGAATCCTCAACGCCTCAACGACGATCTCGATGCGACATGGGAAGTTCTCGCTGAGCCTGTGCAAACCGTGATGCGA CGGTATGGGATCGAGAATCCGTACGAACAGCTCAAGGAACTCACGCGTGGCAAGGGCATCACGCGGGAGGCGCTGCAGAC TTTCGTCAACGGGCTTGCTATTCCTGCTGACGCCAAAGAGCGGCTTCTTGCGATGACGCCAGGGTCGTATGTCGGCAAGG CTGCCGAGTTGGCCAAGCGGATTAAATAA
Upstream 100 bases:
>100_bases GCGCACGCGGCAGGGCCGATGTCCTAAAGTGGTAAAATTCGCGGTTCTCCCCGCTGATATCCGGCAATCGTATTTTCACC GTTCCTGCCTGCCTCCGATC
Downstream 100 bases:
>100_bases TCGGTTTTTGTTGTCTGTGCCGTCTGGACGCTGGTGTTGGTTTTCGCCTTCGCTGGCATGCGCGTTTTGCTTCTGGTTCG CTGGCGTTGCTCCTCTGCGG
Product: adenylosuccinate lyase
Products: NA
Alternate protein names: ASL; Adenylosuccinase; ASase [H]
Number of amino acids: Translated: 462; Mature: 461
Protein sequence:
>462_residues MSDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEIHWLIALSHAGFAEVPRFSAASEQFLLQLAERFT AHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELERASEFIHFACTSEDINNTSHGLMLAGAREHVILPALRAVHQRLV ALAHAQADQPMLSRTHGQPASPTTLGKEIANVAARLSRAIDRIAKVELLGKMNGAVGNFNAHLSAYPEFDWEAFSKEVVE QRLKLTFNPYTIQIEPHDYMAELFDAISRANTILLDLDRDVWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNL GLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDALNRGLDKLEVNPQRLNDDLDATWEVLAEPVQTVMR RYGIENPYEQLKELTRGKGITREALQTFVNGLAIPADAKERLLAMTPGSYVGKAAELAKRIK
Sequences:
>Translated_462_residues MSDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEIHWLIALSHAGFAEVPRFSAASEQFLLQLAERFT AHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELERASEFIHFACTSEDINNTSHGLMLAGAREHVILPALRAVHQRLV ALAHAQADQPMLSRTHGQPASPTTLGKEIANVAARLSRAIDRIAKVELLGKMNGAVGNFNAHLSAYPEFDWEAFSKEVVE QRLKLTFNPYTIQIEPHDYMAELFDAISRANTILLDLDRDVWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNL GLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDALNRGLDKLEVNPQRLNDDLDATWEVLAEPVQTVMR RYGIENPYEQLKELTRGKGITREALQTFVNGLAIPADAKERLLAMTPGSYVGKAAELAKRIK >Mature_461_residues SDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEIHWLIALSHAGFAEVPRFSAASEQFLLQLAERFTA HDAARIKDIERVTNHDVKAVEYWLKESVKGQPELERASEFIHFACTSEDINNTSHGLMLAGAREHVILPALRAVHQRLVA LAHAQADQPMLSRTHGQPASPTTLGKEIANVAARLSRAIDRIAKVELLGKMNGAVGNFNAHLSAYPEFDWEAFSKEVVEQ RLKLTFNPYTIQIEPHDYMAELFDAISRANTILLDLDRDVWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLG LANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDALNRGLDKLEVNPQRLNDDLDATWEVLAEPVQTVMRR YGIENPYEQLKELTRGKGITREALQTFVNGLAIPADAKERLLAMTPGSYVGKAAELAKRIK
Specific function: De novo purine biosynthesis; eighth step. [C]
COG id: COG0015
COG function: function code F; Adenylosuccinate lyase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]
Homologues:
Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=62.4175824175824, Blast_Score=588, Evalue=1e-169,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013539 - InterPro: IPR000362 - InterPro: IPR020557 - InterPro: IPR008948 - InterPro: IPR022761 - InterPro: IPR004769 [H]
Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]
EC number: =4.3.2.2 [H]
Molecular weight: Translated: 51648; Mature: 51517
Theoretical pI: Translated: 6.69; Mature: 6.69
Prosite motif: PS00163 FUMARATE_LYASES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEIHWLIALSHAGFAE CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCHHH VPRFSAASEQFLLQLAERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELERASE CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH FIHFACTSEDINNTSHGLMLAGAREHVILPALRAVHQRLVALAHAQADQPMLSRTHGQPA HEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC SPTTLGKEIANVAARLSRAIDRIAKVELLGKMNGAVGNFNAHLSAYPEFDWEAFSKEVVE CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHH QRLKLTFNPYTIQIEPHDYMAELFDAISRANTILLDLDRDVWGYISLGYFKQKTKAGEIG HHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSL CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH LAYDALNRGLDKLEVNPQRLNDDLDATWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGI HHHHHHHCCCHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC TREALQTFVNGLAIPADAKERLLAMTPGSYVGKAAELAKRIK CHHHHHHHHCCCCCCCCHHHCEEEECCCHHHHHHHHHHHHCC >Mature Secondary Structure SDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEIHWLIALSHAGFAE CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCHHH VPRFSAASEQFLLQLAERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELERASE CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH FIHFACTSEDINNTSHGLMLAGAREHVILPALRAVHQRLVALAHAQADQPMLSRTHGQPA HEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC SPTTLGKEIANVAARLSRAIDRIAKVELLGKMNGAVGNFNAHLSAYPEFDWEAFSKEVVE CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHH QRLKLTFNPYTIQIEPHDYMAELFDAISRANTILLDLDRDVWGYISLGYFKQKTKAGEIG HHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSL CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH LAYDALNRGLDKLEVNPQRLNDDLDATWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGI HHHHHHHCCCHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC TREALQTFVNGLAIPADAKERLLAMTPGSYVGKAAELAKRIK CHHHHHHHHCCCCCCCCHHHCEEEECCCHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]