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The map label for this gene is pyrC [H]

Identifier: 186477315

GI number: 186477315

Start: 2887463

End: 2888527

Strand: Reverse

Name: pyrC [H]

Synonym: Bphy_2567

Alternate gene names: 186477315

Gene position: 2888527-2887463 (Counterclockwise)

Preceding gene: 186477317

Following gene: 186477314

Centisome position: 83.02

GC content: 64.6

Gene sequence:

>1065_bases
ATGACTGCCTCTACCGCTGCTTCCGTCGATACCCTCACGTTCGCCCGTCCCGATGACTGGCACCTGCACGTCCGCGATGG
CGCGATGCTCGCCGCTGTTCTGCCGCACACTGCGCGCCAGTTCGGCCGCGCGATCATCATGCCGAACCTGAAGCCGCCCG
TCACGACGACGGAAATGGCGCGGGCGTATCGCGAGCGCATCGTGGCGGCGATTCCCGCCGGCGTGAAGTTCGAGCCGCTG
ATGACGTTGTATCTCACCGACAACACCCCGCCCGAAGAAATCCGCCGGGCACGCGAGAGCGGCTTCGTGCATGGCGTGAA
GCTCTACCCGGCGGGTGCGACGACGAACTCGGACGCAGGCGTCACCGACATCCGCAAGTGCGCGAAGACGTTGGAGATGA
TGCAGGAAACGGGCATGCCGCTGCTCGTGCATGGCGAAGTGACGGATTCGTCGATCGATCTGTTCGACCGCGAGAAAGTG
TTCATCGACCGCGTGATGACGCCGCTGCGCCGCGATTTCCCAGCGTTGAAAGTCGTGTTCGAGCACATCACGACAAAAGA
TGCCGCCGACTACGTACGCGATTCGGGCGCGCCGCCTGAACTGCTCGGCGCGACGATTACCGCGCATCACCTGCTGTACA
ACCGCAACGCGATCTTCCAGGGCGGCATTCGTCCGCATTACTACTGCCTGCCTGTGTTGAAGCGCGAAACGCATCGCGTG
GCGCTCGTCGAGGCGGCGACGTCGGGCAATCCGCGCTTCTTCCTCGGCACGGACAGCGCACCACATCCGAAGGGCCTGAA
GGAACACGCGTGCGGCTGCGCGGGTTGCTACACGGCGCTGCATGCGCTCGAGCTGTACGGGGAAGCGTTCGACACGGCGG
GCGCGCTCGACAAGCTGGAAGGTTTTGCCAGCTTCCACGGTGCGGATTTCTACCGTCTGCCGCGCAGCGAAGAGACAGTG
ACGCTGCGCCGCGAACAGTGGACGCTGCCCGCGGAGCTCGAGGCTGGTGATACGCCCGTCGTGCCATTGCGCGGCGGCGA
AGCGATCGGCTGGAAGCTCATCTGA

Upstream 100 bases:

>100_bases
ACGGCAAAAAGCAAAATTGACGGGTGTTTTGGCTGCATTTGTGGAAAGCGCTCGTCAGTTATGCTGTTTGCCGTGTCCGC
GCCGTTCGCGAGTTCCCGCC

Downstream 100 bases:

>100_bases
GGGCTTGGCTGACGATATGTCGTGCGGTGCAGTTTCACCGGATGGGGCGCTGAAACGCGCCGCCCCGGGTTTCGCGGATA
TCGACTGGTCGCGGCCGTGG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTASTAASVDTLTFARPDDWHLHVRDGAMLAAVLPHTARQFGRAIIMPNLKPPVTTTEMARAYRERIVAAIPAGVKFEPL
MTLYLTDNTPPEEIRRARESGFVHGVKLYPAGATTNSDAGVTDIRKCAKTLEMMQETGMPLLVHGEVTDSSIDLFDREKV
FIDRVMTPLRRDFPALKVVFEHITTKDAADYVRDSGAPPELLGATITAHHLLYNRNAIFQGGIRPHYYCLPVLKRETHRV
ALVEAATSGNPRFFLGTDSAPHPKGLKEHACGCAGCYTALHALELYGEAFDTAGALDKLEGFASFHGADFYRLPRSEETV
TLRREQWTLPAELEAGDTPVVPLRGGEAIGWKLI

Sequences:

>Translated_354_residues
MTASTAASVDTLTFARPDDWHLHVRDGAMLAAVLPHTARQFGRAIIMPNLKPPVTTTEMARAYRERIVAAIPAGVKFEPL
MTLYLTDNTPPEEIRRARESGFVHGVKLYPAGATTNSDAGVTDIRKCAKTLEMMQETGMPLLVHGEVTDSSIDLFDREKV
FIDRVMTPLRRDFPALKVVFEHITTKDAADYVRDSGAPPELLGATITAHHLLYNRNAIFQGGIRPHYYCLPVLKRETHRV
ALVEAATSGNPRFFLGTDSAPHPKGLKEHACGCAGCYTALHALELYGEAFDTAGALDKLEGFASFHGADFYRLPRSEETV
TLRREQWTLPAELEAGDTPVVPLRGGEAIGWKLI
>Mature_353_residues
TASTAASVDTLTFARPDDWHLHVRDGAMLAAVLPHTARQFGRAIIMPNLKPPVTTTEMARAYRERIVAAIPAGVKFEPLM
TLYLTDNTPPEEIRRARESGFVHGVKLYPAGATTNSDAGVTDIRKCAKTLEMMQETGMPLLVHGEVTDSSIDLFDREKVF
IDRVMTPLRRDFPALKVVFEHITTKDAADYVRDSGAPPELLGATITAHHLLYNRNAIFQGGIRPHYYCLPVLKRETHRVA
LVEAATSGNPRFFLGTDSAPHPKGLKEHACGCAGCYTALHALELYGEAFDTAGALDKLEGFASFHGADFYRLPRSEETVT
LRREQWTLPAELEAGDTPVVPLRGGEAIGWKLI

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=347, Percent_Identity=53.8904899135447, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323452, Length=357, Percent_Identity=35.8543417366947, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 38975; Mature: 38844

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTAASVDTLTFARPDDWHLHVRDGAMLAAVLPHTARQFGRAIIMPNLKPPVTTTEMA
CCCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHH
RAYRERIVAAIPAGVKFEPLMTLYLTDNTPPEEIRRARESGFVHGVKLYPAGATTNSDAG
HHHHHHHHHHCCCCCEECCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
VTDIRKCAKTLEMMQETGMPLLVHGEVTDSSIDLFDREKVFIDRVMTPLRRDFPALKVVF
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
EHITTKDAADYVRDSGAPPELLGATITAHHLLYNRNAIFQGGIRPHYYCLPVLKRETHRV
HHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHCCCCEE
ALVEAATSGNPRFFLGTDSAPHPKGLKEHACGCAGCYTALHALELYGEAFDTAGALDKLE
EEEEEECCCCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFASFHGADFYRLPRSEETVTLRREQWTLPAELEAGDTPVVPLRGGEAIGWKLI
HHHHHCCCCEEECCCCCCEEEEEHHCCCCCCCCCCCCCCEEEECCCCCCCEEEC
>Mature Secondary Structure 
TASTAASVDTLTFARPDDWHLHVRDGAMLAAVLPHTARQFGRAIIMPNLKPPVTTTEMA
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHH
RAYRERIVAAIPAGVKFEPLMTLYLTDNTPPEEIRRARESGFVHGVKLYPAGATTNSDAG
HHHHHHHHHHCCCCCEECCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
VTDIRKCAKTLEMMQETGMPLLVHGEVTDSSIDLFDREKVFIDRVMTPLRRDFPALKVVF
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
EHITTKDAADYVRDSGAPPELLGATITAHHLLYNRNAIFQGGIRPHYYCLPVLKRETHRV
HHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHCCCCEE
ALVEAATSGNPRFFLGTDSAPHPKGLKEHACGCAGCYTALHALELYGEAFDTAGALDKLE
EEEEEECCCCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFASFHGADFYRLPRSEETVTLRREQWTLPAELEAGDTPVVPLRGGEAIGWKLI
HHHHHCCCCEEECCCCCCEEEEEHHCCCCCCCCCCCCCCEEEECCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA