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The map label for this gene is tyrS [H]

Identifier: 186477308

GI number: 186477308

Start: 2880909

End: 2882207

Strand: Reverse

Name: tyrS [H]

Synonym: Bphy_2560

Alternate gene names: 186477308

Gene position: 2882207-2880909 (Counterclockwise)

Preceding gene: 186477310

Following gene: 186477307

Centisome position: 82.84

GC content: 61.35

Gene sequence:

>1299_bases
ATGCGCCGCAACTATGGGACAATCTCTCTTTTTGCGATCTCCCGTTCCAGCATGAGCACCGAGTTCACCGCCAAGCCCAG
TTCCCCCTATCCGATCACCGACCAGGTCCTTCATGCGCTCGAAGTCACGAAGCGCGGCGTCGACGAACTGCTGATCGAAG
AGGAGTTCGCGCAAAAGCTCGCGAGGAGCGCTGCCACGGGCACGCCGCTGCGCATCAAGCTCGGTCTGGATCCGACGGCG
CCCGACATTCACATCGGTCATACCGTGGTGCTGAACAAGATGCGCCAGTTGCAGGATCTCGGCCACACGGTGATTTTCCT
GATCGGCGATTTCACGTCGCTGATCGGCGATCCTTCGGGCCGCAATGCAACGCGCCCGCCGCTCACGCGCGAGCAGATCG
AATCGAACGCGAAGACCTACTTCGAACAGGCTGCGCTGGTGCTCGACCGCGCGAAGACGGAAATCCGTTACAACAGCGAA
TGGTCGATGCCGCTCGGTGCCGACGGCATGATCAAGCTCGCATCGCGCTACACCGTCGCGCGCATCCTGGAGCGCGAAGA
CTTTACGAAGCGTTTTCAGAGCGGCGTGCCCATCTCGATCCACGAATTCCTCTACCCGCTGATGCAGGGTTACGACTCGG
TGGCGCTGAATGCCGACCTGGAACTCGGCGGCACGGACCAGAAGTTCAACCTGCTGGTCGGCCGTGAATTGCAGAAGCAG
TACGGCCAGGAACAGCAGTGCATCCTGACGATGCCGCTGCTCGAAGGGCTGGACGGCGTCGAGAAGATGTCGAAGTCGAA
GGGCAACTACGTCGGTATCAGCGAAAAGCCGAATGACATGTTCGGCAAGCTAATGAGCATCTCCGACGTGCTGATGTGGC
GGTACTTCGAGCTACTGTCTTTCCGTCCTATGTCGGAAATCGAGGGCTTCAAGCGAGAGATCGACGGCGGACGCAATCCG
CGCGACTTCAAGGTACTGCTTGCGCAGGAAATCGTCGCTCGCTTCCACTCGCAGGCCGACGCTGAGCGCGCTCTCGAAGA
CTTCAACCACCGCGCGAAGGGCGGCGTGCCGGACGACATCCCGACGGTGACGCTCGCGGGCGCGCCGCTGGCCATTGGCC
AGTTGCTGAAACAGGCGGGCCTCGTGCCGTCGACGAGTGAAGCGCTGCGCAACATCGAACAGGGCGGCGTGAAGATCGAC
GGCGCAACTGTGTCGGACAAGGGCCTGAAGGTCGAGGCGGGCGAGTTCGTCGTGCAGGTCGGCAAACGACGTTTTGCGCG
TGTCACGCTGACGGCATGA

Upstream 100 bases:

>100_bases
GCATCACAACCTGGCCGATCGGCCAGGGTGCGCGGCGCCGAGAATTGCGGAGAATACGCTTCGATGCAGTTCCAGACGTT
TGCAGAGAGTTGAGGGCGCA

Downstream 100 bases:

>100_bases
TGCTCAGTCAGCCTGTGCACATGCCACCACGCGCCCGCGTCGCATGATCGCGCTGATTCAACGTGTGCGGCGCGCCGAAG
TGCGCGTCGGCGATCGCGTG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MRRNYGTISLFAISRSSMSTEFTAKPSSPYPITDQVLHALEVTKRGVDELLIEEEFAQKLARSAATGTPLRIKLGLDPTA
PDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKTYFEQAALVLDRAKTEIRYNSE
WSMPLGADGMIKLASRYTVARILEREDFTKRFQSGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQ
YGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPNDMFGKLMSISDVLMWRYFELLSFRPMSEIEGFKREIDGGRNP
RDFKVLLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPTVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKID
GATVSDKGLKVEAGEFVVQVGKRRFARVTLTA

Sequences:

>Translated_432_residues
MRRNYGTISLFAISRSSMSTEFTAKPSSPYPITDQVLHALEVTKRGVDELLIEEEFAQKLARSAATGTPLRIKLGLDPTA
PDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKTYFEQAALVLDRAKTEIRYNSE
WSMPLGADGMIKLASRYTVARILEREDFTKRFQSGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQ
YGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPNDMFGKLMSISDVLMWRYFELLSFRPMSEIEGFKREIDGGRNP
RDFKVLLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPTVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKID
GATVSDKGLKVEAGEFVVQVGKRRFARVTLTA
>Mature_432_residues
MRRNYGTISLFAISRSSMSTEFTAKPSSPYPITDQVLHALEVTKRGVDELLIEEEFAQKLARSAATGTPLRIKLGLDPTA
PDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKTYFEQAALVLDRAKTEIRYNSE
WSMPLGADGMIKLASRYTVARILEREDFTKRFQSGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQ
YGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPNDMFGKLMSISDVLMWRYFELLSFRPMSEIEGFKREIDGGRNP
RDFKVLLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPTVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKID
GATVSDKGLKVEAGEFVVQVGKRRFARVTLTA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=398, Percent_Identity=26.1306532663317, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=28.6729857819905, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=210, Percent_Identity=29.5238095238095, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325160, Length=406, Percent_Identity=25.3694581280788, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130383, Length=450, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47905; Mature: 47905

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNYGTISLFAISRSSMSTEFTAKPSSPYPITDQVLHALEVTKRGVDELLIEEEFAQKL
CCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSAATGTPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSG
HHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCC
RNATRPPLTREQIESNAKTYFEQAALVLDRAKTEIRYNSEWSMPLGADGMIKLASRYTVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
RILEREDFTKRFQSGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQ
HHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHHHHHHHH
YGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPNDMFGKLMSISDVLMWRYFELLS
CCCCCCEEEEHHHHHCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
FRPMSEIEGFKREIDGGRNPRDFKVLLAQEIVARFHSQADAERALEDFNHRAKGGVPDDI
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PTVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKIDGATVSDKGLKVEAGEFVVQV
CEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCEECCCCCEEEHHHHHHHH
GKRRFARVTLTA
HHHHEEEEEECC
>Mature Secondary Structure
MRRNYGTISLFAISRSSMSTEFTAKPSSPYPITDQVLHALEVTKRGVDELLIEEEFAQKL
CCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSAATGTPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSG
HHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCC
RNATRPPLTREQIESNAKTYFEQAALVLDRAKTEIRYNSEWSMPLGADGMIKLASRYTVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
RILEREDFTKRFQSGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQ
HHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHHHHHHHH
YGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPNDMFGKLMSISDVLMWRYFELLS
CCCCCCEEEEHHHHHCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
FRPMSEIEGFKREIDGGRNPRDFKVLLAQEIVARFHSQADAERALEDFNHRAKGGVPDDI
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PTVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKIDGATVSDKGLKVEAGEFVVQV
CEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCEECCCCCEEEHHHHHHHH
GKRRFARVTLTA
HHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA