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The map label for this gene is folK [H]

Identifier: 186477241

GI number: 186477241

Start: 2803061

End: 2803651

Strand: Direct

Name: folK [H]

Synonym: Bphy_2492

Alternate gene names: 186477241

Gene position: 2803061-2803651 (Clockwise)

Preceding gene: 186477240

Following gene: 186477242

Centisome position: 80.57

GC content: 63.79

Gene sequence:

>591_bases
ATGACGGTTGCCTATCTCGGCCTCGGCGCGAATCTCGGGGACGCGCGCCAGACCCTGAAAGACGCGGTGGTGTGTCTCGC
ACAACAGCACACCATCACCGTGCTCGCGAAATCGAGCCTTTATCGAACCGCCCCGATCGACGCGGGCGGCGACGACTATC
TGAATCTCGTCGTCAAGCTCGACACCTCGATGCCCGTGCGGCATCTGCTCGCGCTGTGCCACAAGATCGAGCATCACTTC
GGCCGGGAACGTCCCTTTCGCAATGCGCCGCGCACGCTTGATCTCGACATTCTGCTGTATGGCGAACACGCCATCGACGA
ACCCGATCTGATCGTGCCGCATCCGCGCATGACGGAGCGCGCATTCGTGCTCGTGCCGCTCGTCGAAATCGAACCGGCGC
TGATCATTCCGCAACGCGGCCGCGCCGATGCGTTTCTGCCAGGCGTCGCCGGTCAGCGCATCGAGAAGATGAAATCTCCC
TGCCAGTGCCTCGCGTCCAATGCGTCCAATGCGGCCAACGCGAGCGACGCAGCCGGCACGCTCGGCATGGCAGACAACGG
GGACGACGCACCCAAAGGCGGCTGCCGATGA

Upstream 100 bases:

>100_bases
AGGAAACCGCGTAACAGACGATGCGAGCCACGACGGCCCGCATGAGGACTGATGCATTTCCGGCGCGCGCCGTCGAACGC
AAGTTGCAGGAAGTTATGCC

Downstream 100 bases:

>100_bases
ACTCCTTGCCGCTGACGGCCCCGCCACTCACCGTGACGGCGCCCGACTTGCATCCACCGCACGGCTATCTGACGATCGAA
GGGCCGATCGGCGTCGGCAA

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MTVAYLGLGANLGDARQTLKDAVVCLAQQHTITVLAKSSLYRTAPIDAGGDDYLNLVVKLDTSMPVRHLLALCHKIEHHF
GRERPFRNAPRTLDLDILLYGEHAIDEPDLIVPHPRMTERAFVLVPLVEIEPALIIPQRGRADAFLPGVAGQRIEKMKSP
CQCLASNASNAANASDAAGTLGMADNGDDAPKGGCR

Sequences:

>Translated_196_residues
MTVAYLGLGANLGDARQTLKDAVVCLAQQHTITVLAKSSLYRTAPIDAGGDDYLNLVVKLDTSMPVRHLLALCHKIEHHF
GRERPFRNAPRTLDLDILLYGEHAIDEPDLIVPHPRMTERAFVLVPLVEIEPALIIPQRGRADAFLPGVAGQRIEKMKSP
CQCLASNASNAANASDAAGTLGMADNGDDAPKGGCR
>Mature_195_residues
TVAYLGLGANLGDARQTLKDAVVCLAQQHTITVLAKSSLYRTAPIDAGGDDYLNLVVKLDTSMPVRHLLALCHKIEHHFG
RERPFRNAPRTLDLDILLYGEHAIDEPDLIVPHPRMTERAFVLVPLVEIEPALIIPQRGRADAFLPGVAGQRIEKMKSPC
QCLASNASNAANASDAAGTLGMADNGDDAPKGGCR

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=42.4460431654676, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324073, Length=142, Percent_Identity=38.7323943661972, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 21098; Mature: 20966

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAYLGLGANLGDARQTLKDAVVCLAQQHTITVLAKSSLYRTAPIDAGGDDYLNLVVKL
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCEEEEEEEE
DTSMPVRHLLALCHKIEHHFGRERPFRNAPRTLDLDILLYGEHAIDEPDLIVPHPRMTER
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCCCC
AFVLVPLVEIEPALIIPQRGRADAFLPGVAGQRIEKMKSPCQCLASNASNAANASDAAGT
EEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LGMADNGDDAPKGGCR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVAYLGLGANLGDARQTLKDAVVCLAQQHTITVLAKSSLYRTAPIDAGGDDYLNLVVKL
EEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCEEEEEEEE
DTSMPVRHLLALCHKIEHHFGRERPFRNAPRTLDLDILLYGEHAIDEPDLIVPHPRMTER
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCCCC
AFVLVPLVEIEPALIIPQRGRADAFLPGVAGQRIEKMKSPCQCLASNASNAANASDAAGT
EEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LGMADNGDDAPKGGCR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 2123867 [H]