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The map label for this gene is pcnB [H]

Identifier: 186477240

GI number: 186477240

Start: 2801396

End: 2803012

Strand: Direct

Name: pcnB [H]

Synonym: Bphy_2491

Alternate gene names: 186477240

Gene position: 2801396-2803012 (Clockwise)

Preceding gene: 186477239

Following gene: 186477241

Centisome position: 80.52

GC content: 65.49

Gene sequence:

>1617_bases
GTGATCAAGAAACTCATTCGCAAGCTGTTCGGACAGGACGCGGAGCCCGCTGACGAGGCCGCGCCTTCCACGGAATCCGA
CGACTCCTCGTTCGACGAGCGCCCGCATACGGCGCGCTCGACTCCCAGGTCATCGAAAGGCGGCGCCGCATCGTCGAAGG
CCGGCTCGCGGCGCAAGCCGGCCGCCGCAACGGCCGCCTGCGAGCCCGATGTGCCCGTCATCATCTCCTCCGATATTCAC
GGCATCGACCCATCGCTGATTTCGCGCAACGCCATCCGCGTGACGGAAGGTCTGCAGCAAGCGGGCTTTCGCGCGTTCAT
CGTCGGCGGCGCGGTGCGCGATCTGCTGCTCGGCATCGCGCCGAAGGACTTCGACGTCGCCACGGACGCCACGCCCGAAC
AGGTGCAGAAGCTGTTCCGGCGCGCCCGCATCATCGGCCGGCGCTTTCAGATCGTGCACGTGCAGTTCGGTCAGGAAATC
ATCGAAACGTCGACCTTCCGCGCGCTCATGGACGCGCCGCCCGCCGATTCTCCCCCGCCTCGCCGCCTGAAGCGCGACGA
GCTGGACCGGCGCACGCATGCCGTCGACGCAAGCGGCCGCGTGCTGCGCGACAACGTGTGGGGCGAGCAGCACGAAGACG
CGACGCGACGCGACTTCACCGTCAACGCGATGTACTACGATCCCGCGACGCAGACGGTGCTCGACTATCACAACGGCATG
GCTGACGTGCGCGCGCGCCTGCTGCGCATGATCGGCGATCCTGCAACGCGCTATCGCGAAGACCCGGTGCGCATGCTGCG
GGTCGTGCGCTTTGCGGCGAAGCTCGGCTTCGAAATCGACGAGGCGACGCGTGCACCGATCACCGAACTCGCCGACCTGA
TCAACAACGTGCCCGCCGCGCGTCTGTTCGACGAGATGCTCAAGCTGCTGCTGTCGGGCCACGCGCTCGCGTGCCTGCAG
CGCCTGCGCAAGGAAGGGCTGCATCACGGCCTGCTGCCGCTGCTCGATGTCGTACTCGAACAGCCGCACGGCGAGAAGTT
CATCACGCTCGCATTGAACAACACCGACGCGCGCGTGCGCGCCGGCAAGCCGGTATCGCCCGGCTTCCTGTTCGCAACGC
TGTTGTGGCACGACATGCAGCAGCGGTGGCAGCAATACGAGGCAAACGGCGAATACCCGGTGCCCGCGCTGCATCGCGCG
ATGGATGACGTGCTGGACATGCAGACCGAGAAGCTCGCTATTCACAAGCGTTTCTCGTCGGATATGCGCGAGATCTGGGG
CCTGCAGCATCGCCTCGAAAAGCGTTCCGGCCGCAGCGCGCTGAAGCTGCTGGAACACCAAAGATTTAGAGCGGGGTATG
ATTTCCTCCTGTTGCGCTGCGAATCGGGCGAACTGGATGAAGCGGTCGGTTCGTGGTGGACGGAGTTCATCGAAGGAGAC
GCCGCTGCGCGCGAAGCACTGCTTTCGCAAGGCGGGAAGGACCGGGCGCCCAGAAAACGACGGCGGCGTAGCAACAGCCG
AAACCGCAGCAAACAGGGCGACGGAATGGAGGGCGGCACGGCTTCAGGAAACCGCGTAACAGACGATGCGAGCCACGACG
GCCCGCATGAGGACTGA

Upstream 100 bases:

>100_bases
TCGAAAAGGTCACCGACCCGATCGCGACCAACCCCGACGACACGCTGCGCGCTCACGCACAGAAGCAAGGCTGGCGCATC
CTCGAACTCTTTCAACCCAC

Downstream 100 bases:

>100_bases
TGCATTTCCGGCGCGCGCCGTCGAACGCAAGTTGCAGGAAGTTATGCCATGACGGTTGCCTATCTCGGCCTCGGCGCGAA
TCTCGGGGACGCGCGCCAGA

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MIKKLIRKLFGQDAEPADEAAPSTESDDSSFDERPHTARSTPRSSKGGAASSKAGSRRKPAAATAACEPDVPVIISSDIH
GIDPSLISRNAIRVTEGLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEI
IETSTFRALMDAPPADSPPPRRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTVNAMYYDPATQTVLDYHNGM
ADVRARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFEIDEATRAPITELADLINNVPAARLFDEMLKLLLSGHALACLQ
RLRKEGLHHGLLPLLDVVLEQPHGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMQQRWQQYEANGEYPVPALHRA
MDDVLDMQTEKLAIHKRFSSDMREIWGLQHRLEKRSGRSALKLLEHQRFRAGYDFLLLRCESGELDEAVGSWWTEFIEGD
AAAREALLSQGGKDRAPRKRRRRSNSRNRSKQGDGMEGGTASGNRVTDDASHDGPHED

Sequences:

>Translated_538_residues
MIKKLIRKLFGQDAEPADEAAPSTESDDSSFDERPHTARSTPRSSKGGAASSKAGSRRKPAAATAACEPDVPVIISSDIH
GIDPSLISRNAIRVTEGLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEI
IETSTFRALMDAPPADSPPPRRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTVNAMYYDPATQTVLDYHNGM
ADVRARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFEIDEATRAPITELADLINNVPAARLFDEMLKLLLSGHALACLQ
RLRKEGLHHGLLPLLDVVLEQPHGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMQQRWQQYEANGEYPVPALHRA
MDDVLDMQTEKLAIHKRFSSDMREIWGLQHRLEKRSGRSALKLLEHQRFRAGYDFLLLRCESGELDEAVGSWWTEFIEGD
AAAREALLSQGGKDRAPRKRRRRSNSRNRSKQGDGMEGGTASGNRVTDDASHDGPHED
>Mature_538_residues
MIKKLIRKLFGQDAEPADEAAPSTESDDSSFDERPHTARSTPRSSKGGAASSKAGSRRKPAAATAACEPDVPVIISSDIH
GIDPSLISRNAIRVTEGLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEI
IETSTFRALMDAPPADSPPPRRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTVNAMYYDPATQTVLDYHNGM
ADVRARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFEIDEATRAPITELADLINNVPAARLFDEMLKLLLSGHALACLQ
RLRKEGLHHGLLPLLDVVLEQPHGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMQQRWQQYEANGEYPVPALHRA
MDDVLDMQTEKLAIHKRFSSDMREIWGLQHRLEKRSGRSALKLLEHQRFRAGYDFLLLRCESGELDEAVGSWWTEFIEGD
AAAREALLSQGGKDRAPRKRRRRSNSRNRSKQGDGMEGGTASGNRVTDDASHDGPHED

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=239, Percent_Identity=28.8702928870293, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI87081691, Length=431, Percent_Identity=45.4756380510441, Blast_Score=344, Evalue=8e-96,
Organism=Escherichia coli, GI1789436, Length=252, Percent_Identity=30.5555555555556, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 60228; Mature: 60228

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLIRKLFGQDAEPADEAAPSTESDDSSFDERPHTARSTPRSSKGGAASSKAGSRRKP
CHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAATAACEPDVPVIISSDIHGIDPSLISRNAIRVTEGLQQAGFRAFIVGGAVRDLLLGIA
CHHCCCCCCCCCEEECCCCCCCCHHHHCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCC
PKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRALMDAPPADSPPP
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCH
RRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTVNAMYYDPATQTVLDYHNGM
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHCCH
ADVRARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFEIDEATRAPITELADLINNVPAA
HHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCHH
RLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLLDVVLEQPHGEKFITLALNNTDARVR
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
AGKPVSPGFLFATLLWHDMQQRWQQYEANGEYPVPALHRAMDDVLDMQTEKLAIHKRFSS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DMREIWGLQHRLEKRSGRSALKLLEHQRFRAGYDFLLLRCESGELDEAVGSWWTEFIEGD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCH
AAAREALLSQGGKDRAPRKRRRRSNSRNRSKQGDGMEGGTASGNRVTDDASHDGPHED
HHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKLIRKLFGQDAEPADEAAPSTESDDSSFDERPHTARSTPRSSKGGAASSKAGSRRKP
CHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAATAACEPDVPVIISSDIHGIDPSLISRNAIRVTEGLQQAGFRAFIVGGAVRDLLLGIA
CHHCCCCCCCCCEEECCCCCCCCHHHHCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCC
PKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRALMDAPPADSPPP
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCH
RRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTVNAMYYDPATQTVLDYHNGM
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHCCH
ADVRARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFEIDEATRAPITELADLINNVPAA
HHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCHH
RLFDEMLKLLLSGHALACLQRLRKEGLHHGLLPLLDVVLEQPHGEKFITLALNNTDARVR
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
AGKPVSPGFLFATLLWHDMQQRWQQYEANGEYPVPALHRAMDDVLDMQTEKLAIHKRFSS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DMREIWGLQHRLEKRSGRSALKLLEHQRFRAGYDFLLLRCESGELDEAVGSWWTEFIEGD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCH
AAAREALLSQGGKDRAPRKRRRRSNSRNRSKQGDGMEGGTASGNRVTDDASHDGPHED
HHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]