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The map label for this gene is nudD [H]

Identifier: 186477227

GI number: 186477227

Start: 2786938

End: 2787354

Strand: Direct

Name: nudD [H]

Synonym: Bphy_2478

Alternate gene names: 186477227

Gene position: 2786938-2787354 (Clockwise)

Preceding gene: 186477226

Following gene: 186477229

Centisome position: 80.1

GC content: 63.55

Gene sequence:

>417_bases
ATGACGCCGCTCGTGTCGATCGACCTGATCGTGTCGGATTACGCAGGCCGCGTGCTGGTCGGCCATCGCCGCAACCGGCC
GGCCCTCGGCACATGGTTCGTGCCGGGCGGACGCATCTGCAAGAACGAAAGGCTCGACGCGGCGTTCACGCGCATCGTCG
ATGCGGAACTCGGCATTGCGGGCATGGAGCGCTCGGCCGCACGCTTCGGCGGCCTGTTCGAACACCTTTATAAGGACAAC
TTCGCTGGCGCCGACGAAATCTCCACGCACTATGTCGTGCTGGCCTATTTCCTGACGCTGGAAAGTACCGCGTCCGTGGG
CCGTTTCGATCAGCACAGCCGCTACATCTGGCTCACGCCCGAAGCGCTGCTCGCGCGCGATGATGTGCACGAGAACACCA
AGGCCTACTTCCGCTGA

Upstream 100 bases:

>100_bases
TGCGCTGCACCCATTAAACAAGACTACATGGCGAGACAGAGACATGGCCGCTACGTGCGCGGATTTTCTCGAAGCAGACG
ATTTTCTTCAGGTCGTGCGA

Downstream 100 bases:

>100_bases
GCCGCACGCGTGCGGTAACGCTTGGGCAACGGAAAGCGCGGCAGACTCTGCCGCCGCGCCCTCTCGAACACGACGCTCAC
ACCCGGCTCAGCAACTCGGC

Product: NUDIX hydrolase

Products: GDP; mannose [C]

Alternate protein names: GDPMH; Colanic acid biosynthesis protein wcaH [H]

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MTPLVSIDLIVSDYAGRVLVGHRRNRPALGTWFVPGGRICKNERLDAAFTRIVDAELGIAGMERSAARFGGLFEHLYKDN
FAGADEISTHYVVLAYFLTLESTASVGRFDQHSRYIWLTPEALLARDDVHENTKAYFR

Sequences:

>Translated_138_residues
MTPLVSIDLIVSDYAGRVLVGHRRNRPALGTWFVPGGRICKNERLDAAFTRIVDAELGIAGMERSAARFGGLFEHLYKDN
FAGADEISTHYVVLAYFLTLESTASVGRFDQHSRYIWLTPEALLARDDVHENTKAYFR
>Mature_137_residues
TPLVSIDLIVSDYAGRVLVGHRRNRPALGTWFVPGGRICKNERLDAAFTRIVDAELGIAGMERSAARFGGLFEHLYKDNF
AGADEISTHYVVLAYFLTLESTASVGRFDQHSRYIWLTPEALLARDDVHENTKAYFR

Specific function: It could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. May be involved in the degradation of GDP-mannose and GDP-glucose, diverting the GDP to the synthesis of GDP-fucos

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI87082031, Length=136, Percent_Identity=50, Blast_Score=135, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021161
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: NA

Molecular weight: Translated: 15535; Mature: 15404

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLVSIDLIVSDYAGRVLVGHRRNRPALGTWFVPGGRICKNERLDAAFTRIVDAELGIA
CCCEEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC
GMERSAARFGGLFEHLYKDNFAGADEISTHYVVLAYFLTLESTASVGRFDQHSRYIWLTP
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECH
EALLARDDVHENTKAYFR
HHHHHHCCCCCCHHHHCC
>Mature Secondary Structure 
TPLVSIDLIVSDYAGRVLVGHRRNRPALGTWFVPGGRICKNERLDAAFTRIVDAELGIA
CCEEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC
GMERSAARFGGLFEHLYKDNFAGADEISTHYVVLAYFLTLESTASVGRFDQHSRYIWLTP
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECH
EALLARDDVHENTKAYFR
HHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-mannose; H2O [C]

Specific reaction: GDP-mannose + H2O = GDP + mannose [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]