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The map label for this gene is gmd [H]

Identifier: 186477220

GI number: 186477220

Start: 2774449

End: 2775576

Strand: Reverse

Name: gmd [H]

Synonym: Bphy_2471

Alternate gene names: 186477220

Gene position: 2775576-2774449 (Counterclockwise)

Preceding gene: 186477221

Following gene: 186477219

Centisome position: 79.78

GC content: 63.03

Gene sequence:

>1128_bases
ATGACTCAGAAGGTTGCCTTGATTACCGGCATTACCGGCCAGGACGGCTCATACCTGGCCGAGCTGTTGATCGACAAGGG
CTACGAGGTGCACGGCATCAAGCGTCGCTCGTCGTTGTTCAACACCGACCGGATCGACCACCTTTACCGCGATGTGCATG
ACCCGGACCAGCGCCTGCAACTGCATCACGGCGACCTGACGGATTCGACGAGCCTGCTGCGCATCATGCAGCGCGTCGAG
CCCGACGAAGTCTATAACCTCGCGGCGCAGAGCCACGTGGCCGTGTCGTTCGAGGAACCCGAATACACCGCCAATGCCGA
CGGACTCGGCACGCTGCGCCTGCTCGAAGCGATCCGCATCTGCGGCTTCGAGAAAAAGACGCGCTTTTATCAGGCATCGA
CCTCCGAACTGTATGGCCTCGTGCAGGAAGTGCCGCAGCGCGAGACCACGCCGTTCTATCCGCGCAGCCCGTACGCGGTG
GCGAAGCTGTTCGCGTACTGGACCACGGTCAACTATCGCGAAGCGTATGGCATCTACGCGTGCAACGGCATTCTGTTCAA
CCACGAGTCGCCCGTGCGCGGCGAGACCTTCGTCACGCGCAAGATCACGCGCGCAATTGCACGCATCGCGGTCGGCCTGC
AAGACCAGCTCTATCTGGGCAATCTGTCCGCGCTGCGCGACTGGGGCCACGCGCGCGATTACGTCGAAATGCAATGGATG
ATGCTGCAGCAGGAGCAGCCCGAAGACTTCGTGATCGCGACGGGCGTGCAGTACAGCGTGCGCGAGTTCGTGCAGCGCGC
GGCCGCTGAACTGGGCATCACGGTGCGTTTCGAAGGCAGCGGCGTGCACGAAGTGGGCATCGTCGAACATGTCGAGCGCC
GCCATCGCGCGGAAATGCGTGTGCAGCCTGGCGATGTGATCGTGCGCGTCGATCCGCGCTACTTCCGCCCGACGGAAGTC
GAGACGCTGCTTGGCGATCCGTCGAAGGCGCAGCAAAGGCTCGGCTGGACACCCGCCACGCCGTTCGAGGCGCTCGTGAA
GGAAATGGTGCTGTCGGACTACCAGATCGCGCGGCGCGATGCGCTCGTCACGCTGGCTGGCTTCAAGGCGCTTGAACATC
ACGAGTGA

Upstream 100 bases:

>100_bases
CGGCAGGGCGGCAAGGGAGCACCCGCGGACATCTACACGAACTGGTGCAAGGGCTGGGGGCCGCGCACAGGCCACAACCC
GGCCGACACAGGAGAATACG

Downstream 100 bases:

>100_bases
TGCCCATGAACAAAAATGCACGCATTTTCGTCGCGGGACATCGCGGGATGGTCGGCTCGGCGCTCGTGCGCAAGCTCGAC
GCGAGCGGCTATCGCAACCT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTQKVALITGITGQDGSYLAELLIDKGYEVHGIKRRSSLFNTDRIDHLYRDVHDPDQRLQLHHGDLTDSTSLLRIMQRVE
PDEVYNLAAQSHVAVSFEEPEYTANADGLGTLRLLEAIRICGFEKKTRFYQASTSELYGLVQEVPQRETTPFYPRSPYAV
AKLFAYWTTVNYREAYGIYACNGILFNHESPVRGETFVTRKITRAIARIAVGLQDQLYLGNLSALRDWGHARDYVEMQWM
MLQQEQPEDFVIATGVQYSVREFVQRAAAELGITVRFEGSGVHEVGIVEHVERRHRAEMRVQPGDVIVRVDPRYFRPTEV
ETLLGDPSKAQQRLGWTPATPFEALVKEMVLSDYQIARRDALVTLAGFKALEHHE

Sequences:

>Translated_375_residues
MTQKVALITGITGQDGSYLAELLIDKGYEVHGIKRRSSLFNTDRIDHLYRDVHDPDQRLQLHHGDLTDSTSLLRIMQRVE
PDEVYNLAAQSHVAVSFEEPEYTANADGLGTLRLLEAIRICGFEKKTRFYQASTSELYGLVQEVPQRETTPFYPRSPYAV
AKLFAYWTTVNYREAYGIYACNGILFNHESPVRGETFVTRKITRAIARIAVGLQDQLYLGNLSALRDWGHARDYVEMQWM
MLQQEQPEDFVIATGVQYSVREFVQRAAAELGITVRFEGSGVHEVGIVEHVERRHRAEMRVQPGDVIVRVDPRYFRPTEV
ETLLGDPSKAQQRLGWTPATPFEALVKEMVLSDYQIARRDALVTLAGFKALEHHE
>Mature_374_residues
TQKVALITGITGQDGSYLAELLIDKGYEVHGIKRRSSLFNTDRIDHLYRDVHDPDQRLQLHHGDLTDSTSLLRIMQRVEP
DEVYNLAAQSHVAVSFEEPEYTANADGLGTLRLLEAIRICGFEKKTRFYQASTSELYGLVQEVPQRETTPFYPRSPYAVA
KLFAYWTTVNYREAYGIYACNGILFNHESPVRGETFVTRKITRAIARIAVGLQDQLYLGNLSALRDWGHARDYVEMQWMM
LQQEQPEDFVIATGVQYSVREFVQRAAAELGITVRFEGSGVHEVGIVEHVERRHRAEMRVQPGDVIVRVDPRYFRPTEVE
TLLGDPSKAQQRLGWTPATPFEALVKEMVLSDYQIARRDALVTLAGFKALEHHE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=359, Percent_Identity=57.9387186629526, Blast_Score=438, Evalue=1e-123,
Organism=Escherichia coli, GI1788366, Length=365, Percent_Identity=71.5068493150685, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1788353, Length=365, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994969, Length=186, Percent_Identity=30.1075268817204, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=362, Percent_Identity=61.6022099447514, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539424, Length=362, Percent_Identity=60.7734806629834, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901790, Length=362, Percent_Identity=60.7734806629834, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901788, Length=362, Percent_Identity=60.7734806629834, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17539422, Length=362, Percent_Identity=60.7734806629834, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901786, Length=362, Percent_Identity=60.7734806629834, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24158427, Length=360, Percent_Identity=58.6111111111111, Blast_Score=439, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 42818; Mature: 42687

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKVALITGITGQDGSYLAELLIDKGYEVHGIKRRSSLFNTDRIDHLYRDVHDPDQRLQ
CCCEEEEEEECCCCCHHHHHHHHHHCCCCEECCHHHHHHCCHHHHHHHHHHCCCHHHHHE
LHHGDLTDSTSLLRIMQRVEPDEVYNLAAQSHVAVSFEEPEYTANADGLGTLRLLEAIRI
EECCCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
CGFEKKTRFYQASTSELYGLVQEVPQRETTPFYPRSPYAVAKLFAYWTTVNYREAYGIYA
CCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCEEE
CNGILFNHESPVRGETFVTRKITRAIARIAVGLQDQLYLGNLSALRDWGHARDYVEMQWM
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHH
MLQQEQPEDFVIATGVQYSVREFVQRAAAELGITVRFEGSGVHEVGIVEHVERRHRAEMR
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCE
VQPGDVIVRVDPRYFRPTEVETLLGDPSKAQQRLGWTPATPFEALVKEMVLSDYQIARRD
ECCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALVTLAGFKALEHHE
HEEEHHHHHHHHCCC
>Mature Secondary Structure 
TQKVALITGITGQDGSYLAELLIDKGYEVHGIKRRSSLFNTDRIDHLYRDVHDPDQRLQ
CCEEEEEEECCCCCHHHHHHHHHHCCCCEECCHHHHHHCCHHHHHHHHHHCCCHHHHHE
LHHGDLTDSTSLLRIMQRVEPDEVYNLAAQSHVAVSFEEPEYTANADGLGTLRLLEAIRI
EECCCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
CGFEKKTRFYQASTSELYGLVQEVPQRETTPFYPRSPYAVAKLFAYWTTVNYREAYGIYA
CCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCEEE
CNGILFNHESPVRGETFVTRKITRAIARIAVGLQDQLYLGNLSALRDWGHARDYVEMQWM
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHH
MLQQEQPEDFVIATGVQYSVREFVQRAAAELGITVRFEGSGVHEVGIVEHVERRHRAEMR
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCE
VQPGDVIVRVDPRYFRPTEVETLLGDPSKAQQRLGWTPATPFEALVKEMVLSDYQIARRD
ECCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALVTLAGFKALEHHE
HEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]