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The map label for this gene is fcl [H]

Identifier: 186477219

GI number: 186477219

Start: 2773448

End: 2774443

Strand: Reverse

Name: fcl [H]

Synonym: Bphy_2470

Alternate gene names: 186477219

Gene position: 2774443-2773448 (Counterclockwise)

Preceding gene: 186477220

Following gene: 186477218

Centisome position: 79.74

GC content: 63.96

Gene sequence:

>996_bases
ATGAACAAAAATGCACGCATTTTCGTCGCGGGACATCGCGGGATGGTCGGCTCGGCGCTCGTGCGCAAGCTCGACGCGAG
CGGCTATCGCAACCTCGTCACGCGCACGCATGCCGAACTCGATCTGACCGATCAGGCCGCCGTGAACCGCTTCTTCGAGT
GCGAGCAGATCGACGTCGTGCTGCTCGCGGCGGCGCGCGTCGGCGGTATTCTCGCCAACGCGACGCAGCCCGGCGAGTTT
CTCTATGAGAACCTCGTGATCGAAACCAACGTGATTCACGCGGCGTATCGCGCGAACGTCGACCGGCTGGTGTTCTTCGG
TTCGTCGTGCATCTACCCGAAGCTGTGTCCGCAGCCGATCCTCGAATCGTATCTGCTGACGTCGGAACTGGAACCGACCA
ACGACGCGTACGCGATCGCGAAGATTGCCGGCCTGAAACTGTGCGACGCCTACAACCGCGAGTACGGCACGCGCTATGTC
GCGCTCATGCCGACCAACCTGTACGGTCCGAACGACAACTACGACCTCAAGAACAGTCATGTGCTGCCGGCGCTGATCCG
CAAGGCGCACGAGGCGCGCCTGCACGGCGACCGGTCTTTGATCGTGTGGGGCAGCGGCACGCCGCGCCGCGAGTTCCTGC
ATGTCGACGATCTCGCGGCGGCGACGCTGTTCGTACTCGAGCACGACGTGAATACGGGTGTGTTCAACGTCGGCGTCGGC
GAAGACCTCAGCATTCGCGAACTCGCGCAGACGGTCTGCGATGTGGTGGGCTTCGAAGGCGATCTGCAGTTCGATGCGAG
CAAGCCCGACGGCACGCCGCGCAAGCTGCTCGATGTGTCGCGGCTGGCGGCGATGGGCTGGCGCGCGAGCATCGGGCTCG
CGGAAGGCATCGGCAACACCTATCGCGAGTTTGCCGCCCGGTATGACGAGAAGTACGGGGACAAGCATGGGGAGACACGC
GCCGCGCCTTCGTTCGCGATGGCTGCGCGTGTCTGA

Upstream 100 bases:

>100_bases
TCGTGAAGGAAATGGTGCTGTCGGACTACCAGATCGCGCGGCGCGATGCGCTCGTCACGCTGGCTGGCTTCAAGGCGCTT
GAACATCACGAGTGATGCCC

Downstream 100 bases:

>100_bases
TGCGCCGTTCCTGGTGACGGACGTTGCCTGGGACCGGGATTAGGGCGACGTCCGTTCTTTTTGCAAGGGCAATCGACAGA
TTCGACCGAATGAGGACTGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNKNARIFVAGHRGMVGSALVRKLDASGYRNLVTRTHAELDLTDQAAVNRFFECEQIDVVLLAAARVGGILANATQPGEF
LYENLVIETNVIHAAYRANVDRLVFFGSSCIYPKLCPQPILESYLLTSELEPTNDAYAIAKIAGLKLCDAYNREYGTRYV
ALMPTNLYGPNDNYDLKNSHVLPALIRKAHEARLHGDRSLIVWGSGTPRREFLHVDDLAAATLFVLEHDVNTGVFNVGVG
EDLSIRELAQTVCDVVGFEGDLQFDASKPDGTPRKLLDVSRLAAMGWRASIGLAEGIGNTYREFAARYDEKYGDKHGETR
AAPSFAMAARV

Sequences:

>Translated_331_residues
MNKNARIFVAGHRGMVGSALVRKLDASGYRNLVTRTHAELDLTDQAAVNRFFECEQIDVVLLAAARVGGILANATQPGEF
LYENLVIETNVIHAAYRANVDRLVFFGSSCIYPKLCPQPILESYLLTSELEPTNDAYAIAKIAGLKLCDAYNREYGTRYV
ALMPTNLYGPNDNYDLKNSHVLPALIRKAHEARLHGDRSLIVWGSGTPRREFLHVDDLAAATLFVLEHDVNTGVFNVGVG
EDLSIRELAQTVCDVVGFEGDLQFDASKPDGTPRKLLDVSRLAAMGWRASIGLAEGIGNTYREFAARYDEKYGDKHGETR
AAPSFAMAARV
>Mature_331_residues
MNKNARIFVAGHRGMVGSALVRKLDASGYRNLVTRTHAELDLTDQAAVNRFFECEQIDVVLLAAARVGGILANATQPGEF
LYENLVIETNVIHAAYRANVDRLVFFGSSCIYPKLCPQPILESYLLTSELEPTNDAYAIAKIAGLKLCDAYNREYGTRYV
ALMPTNLYGPNDNYDLKNSHVLPALIRKAHEARLHGDRSLIVWGSGTPRREFLHVDDLAAATLFVLEHDVNTGVFNVGVG
EDLSIRELAQTVCDVVGFEGDLQFDASKPDGTPRKLLDVSRLAAMGWRASIGLAEGIGNTYREFAARYDEKYGDKHGETR
AAPSFAMAARV

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=286, Percent_Identity=34.6153846153846, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=26.0606060606061, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI42516563, Length=313, Percent_Identity=26.1980830670927, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788365, Length=317, Percent_Identity=57.4132492113565, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=24.0469208211144, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=284, Percent_Identity=32.7464788732394, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922778, Length=320, Percent_Identity=30.625, Blast_Score=141, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36425; Mature: 36425

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNARIFVAGHRGMVGSALVRKLDASGYRNLVTRTHAELDLTDQAAVNRFFECEQIDVV
CCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHH
LLAAARVGGILANATQPGEFLYENLVIETNVIHAAYRANVDRLVFFGSSCIYPKLCPQPI
EEEHHHHCCEEECCCCCHHHHHHHEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHH
LESYLLTSELEPTNDAYAIAKIAGLKLCDAYNREYGTRYVALMPTNLYGPNDNYDLKNSH
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC
VLPALIRKAHEARLHGDRSLIVWGSGTPRREFLHVDDLAAATLFVLEHDVNTGVFNVGVG
CHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEEEHHHHHHHEEEEEEECCCCCEEECCCC
EDLSIRELAQTVCDVVGFEGDLQFDASKPDGTPRKLLDVSRLAAMGWRASIGLAEGIGNT
CCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHCCHH
YREFAARYDEKYGDKHGETRAAPSFAMAARV
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MNKNARIFVAGHRGMVGSALVRKLDASGYRNLVTRTHAELDLTDQAAVNRFFECEQIDVV
CCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHH
LLAAARVGGILANATQPGEFLYENLVIETNVIHAAYRANVDRLVFFGSSCIYPKLCPQPI
EEEHHHHCCEEECCCCCHHHHHHHEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHH
LESYLLTSELEPTNDAYAIAKIAGLKLCDAYNREYGTRYVALMPTNLYGPNDNYDLKNSH
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC
VLPALIRKAHEARLHGDRSLIVWGSGTPRREFLHVDDLAAATLFVLEHDVNTGVFNVGVG
CHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEEEHHHHHHHEEEEEEECCCCCEEECCCC
EDLSIRELAQTVCDVVGFEGDLQFDASKPDGTPRKLLDVSRLAAMGWRASIGLAEGIGNT
CCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHCCHH
YREFAARYDEKYGDKHGETRAAPSFAMAARV
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]