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The map label for this gene is wcaL [C]

Identifier: 186477218

GI number: 186477218

Start: 2772199

End: 2773341

Strand: Reverse

Name: wcaL [C]

Synonym: Bphy_2469

Alternate gene names: 186477218

Gene position: 2773341-2772199 (Counterclockwise)

Preceding gene: 186477219

Following gene: 186477217

Centisome position: 79.71

GC content: 64.65

Gene sequence:

>1143_bases
ATGAGCGCGTCAGTCACGATCTTCCACAACGTTGTGTGGTCACGGCACAAGGGCGCCGTGTTCTCCGCGTTGCACAACAT
CTCGGCATCGGGGGCGATCCGCTATTCGATGGTGCAGATCGCGGACACGGAGCATGATCGGATCGGTTTTTCGGACGTCG
ACTATTCGTTTCATCGTTATCCGATGCACAAGCTGTTCGACGGCTGCTACGAGGACGTGCCGCGCTGGCGTCTGACGCTG
CGCCTCGTGTGGGAAGTGCTGAAGGCGAAGTCGGATCTGATCGTGCTGCCCGGCTATCACCGCCCCGAATACTGGGCGAT
GCTCGCCGCGTGCATTGTGACGGGCAAGCGGCGTGCCGTGTTCTGCGATTCGACCGCGCGCGACCGTCCAAAGCGGCTCA
TCACGTCGATTCCGAAGCGCGTGTTTTTTTCGCTGTGCGACGGCTACTTCGGTTTCGGCGAGCGCAGCCGCGATTATCTG
ATGTCGCTTGGGGCGAAGCGCGAGAAGATTTTCATTCCGTGCCAGGCGGCCGCGCTGCCCGTCACGTTTTCGCCGGAGCG
CGCGCTCGCCGAGCGTGTGCGATACCGCGCGGGCAACCCGAATGTGTTTCTGTACGTCGGGCGGCTGTCGGAAGAGAAGG
GCATCACGACGTTGATCGACGCGTTCGCCGGCATCGCGAAGCGCCTGTCCGATCCGCGATTGCGCATCGTCGGCACGGGC
CCGCTCGAACGCGACTTGCGCAAGCGCGTGACGGACCTGGGCCTCGACGGGGCGATCACCTTCGTCGGCAGTCTTCAGGA
CGAGCCGCTGTCGCGCGAATACTACGGCGCGACCTGCATGGTGCTGCCGAGCCGAAGCGAACCCTGGGGGCTCGTCGTCA
ACGAGTCGCTCGCGCACGGCTGTCCCGTCGTCGTCAGCGAGAGCTGCGGGTGCGTGCCCGAACTCGTGCACGAAGGCGAG
AGCGGCTACGCGTTCACGGCGGGGGACGTGGCAGGCCTTCAGCGCACGCTGCTGAAGGCAACGGAAGCATTCGCCGATGC
GCAAGCGTCGGCTCATCGCTGCATGGACGTGATACGGCGTTTCGATCCGCCGTCAGCGGCGGCCAACATTGCGCGCGGCT
GCGCGTTGATGCTGGGCGCCTGA

Upstream 100 bases:

>100_bases
GTTCCTGGTGACGGACGTTGCCTGGGACCGGGATTAGGGCGACGTCCGTTCTTTTTGCAAGGGCAATCGACAGATTCGAC
CGAATGAGGACTGCAAGAAC

Downstream 100 bases:

>100_bases
GATCGGAGAAGGCACGCGCGGCCTGAATCACGCAGGTCGGGGCCGCGTCGGTACGGGGAAGGCGTAGCGCTGTCCGCTGC
CGCATGACGGACACACGTCG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSASVTIFHNVVWSRHKGAVFSALHNISASGAIRYSMVQIADTEHDRIGFSDVDYSFHRYPMHKLFDGCYEDVPRWRLTL
RLVWEVLKAKSDLIVLPGYHRPEYWAMLAACIVTGKRRAVFCDSTARDRPKRLITSIPKRVFFSLCDGYFGFGERSRDYL
MSLGAKREKIFIPCQAAALPVTFSPERALAERVRYRAGNPNVFLYVGRLSEEKGITTLIDAFAGIAKRLSDPRLRIVGTG
PLERDLRKRVTDLGLDGAITFVGSLQDEPLSREYYGATCMVLPSRSEPWGLVVNESLAHGCPVVVSESCGCVPELVHEGE
SGYAFTAGDVAGLQRTLLKATEAFADAQASAHRCMDVIRRFDPPSAAANIARGCALMLGA

Sequences:

>Translated_380_residues
MSASVTIFHNVVWSRHKGAVFSALHNISASGAIRYSMVQIADTEHDRIGFSDVDYSFHRYPMHKLFDGCYEDVPRWRLTL
RLVWEVLKAKSDLIVLPGYHRPEYWAMLAACIVTGKRRAVFCDSTARDRPKRLITSIPKRVFFSLCDGYFGFGERSRDYL
MSLGAKREKIFIPCQAAALPVTFSPERALAERVRYRAGNPNVFLYVGRLSEEKGITTLIDAFAGIAKRLSDPRLRIVGTG
PLERDLRKRVTDLGLDGAITFVGSLQDEPLSREYYGATCMVLPSRSEPWGLVVNESLAHGCPVVVSESCGCVPELVHEGE
SGYAFTAGDVAGLQRTLLKATEAFADAQASAHRCMDVIRRFDPPSAAANIARGCALMLGA
>Mature_379_residues
SASVTIFHNVVWSRHKGAVFSALHNISASGAIRYSMVQIADTEHDRIGFSDVDYSFHRYPMHKLFDGCYEDVPRWRLTLR
LVWEVLKAKSDLIVLPGYHRPEYWAMLAACIVTGKRRAVFCDSTARDRPKRLITSIPKRVFFSLCDGYFGFGERSRDYLM
SLGAKREKIFIPCQAAALPVTFSPERALAERVRYRAGNPNVFLYVGRLSEEKGITTLIDAFAGIAKRLSDPRLRIVGTGP
LERDLRKRVTDLGLDGAITFVGSLQDEPLSREYYGATCMVLPSRSEPWGLVVNESLAHGCPVVVSESCGCVPELVHEGES
GYAFTAGDVAGLQRTLLKATEAFADAQASAHRCMDVIRRFDPPSAAANIARGCALMLGA

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42103; Mature: 41972

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASVTIFHNVVWSRHKGAVFSALHNISASGAIRYSMVQIADTEHDRIGFSDVDYSFHRY
CCCCEEEHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHC
PMHKLFDGCYEDVPRWRLTLRLVWEVLKAKSDLIVLPGYHRPEYWAMLAACIVTGKRRAV
CHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEE
FCDSTARDRPKRLITSIPKRVFFSLCDGYFGFGERSRDYLMSLGAKREKIFIPCQAAALP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCCEEC
VTFSPERALAERVRYRAGNPNVFLYVGRLSEEKGITTLIDAFAGIAKRLSDPRLRIVGTG
EEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECC
PLERDLRKRVTDLGLDGAITFVGSLQDEPLSREYYGATCMVLPSRSEPWGLVVNESLAHG
CHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCEEEEEECCCCCCCEEEEECHHHCC
CPVVVSESCGCVPELVHEGESGYAFTAGDVAGLQRTLLKATEAFADAQASAHRCMDVIRR
CCEEECCCCCCHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDPPSAAANIARGCALMLGA
CCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SASVTIFHNVVWSRHKGAVFSALHNISASGAIRYSMVQIADTEHDRIGFSDVDYSFHRY
CCCEEEHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHC
PMHKLFDGCYEDVPRWRLTLRLVWEVLKAKSDLIVLPGYHRPEYWAMLAACIVTGKRRAV
CHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEE
FCDSTARDRPKRLITSIPKRVFFSLCDGYFGFGERSRDYLMSLGAKREKIFIPCQAAALP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCCEEC
VTFSPERALAERVRYRAGNPNVFLYVGRLSEEKGITTLIDAFAGIAKRLSDPRLRIVGTG
EEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECC
PLERDLRKRVTDLGLDGAITFVGSLQDEPLSREYYGATCMVLPSRSEPWGLVVNESLAHG
CHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCEEEEEECCCCCCCEEEEECHHHCC
CPVVVSESCGCVPELVHEGESGYAFTAGDVAGLQRTLLKATEAFADAQASAHRCMDVIRR
CCEEECCCCCCHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDPPSAAANIARGCALMLGA
CCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]