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The map label for this gene is wcaI [H]

Identifier: 186477217

GI number: 186477217

Start: 2770779

End: 2772086

Strand: Reverse

Name: wcaI [H]

Synonym: Bphy_2468

Alternate gene names: 186477217

Gene position: 2772086-2770779 (Counterclockwise)

Preceding gene: 186477218

Following gene: 186477215

Centisome position: 79.68

GC content: 66.06

Gene sequence:

>1308_bases
ATGAAAATCCTGATCTATGGCCTCAACTACGCGCCCGAGCTGTCGGGTGCGGGTAAATATACGGCCGAGATGGCGCAGAT
GCTGGTGGAGTGCGGTCATGACGTTCGCGTTATCTGCGCGCCTCCGTACTATCCGGACTGGAAAGTCGCGCAGGGCTACT
CGATGTGGCGCTATCGGCGTGACGTGATGCGCGGGGTGCACATCTGGCGCGCGCCGTTGTGGGTGCCGCCGCGCCCAGGC
GGCCTGAAGCGCATGGTGCATCTGGCGTCGTTCGCGCTGGCGTCGCTGCCGTTGCTCGCACGGCAGATCGCGTGGCGGCC
CGGCGCGGTGATGCTGATCGCGCCGACGATGGCCTGCGCCCCCGGCGCGCTGGCGCTCGCACGCGTGACAGGTGCGAAGG
CATGGCTGCATATCCAGGACTACGAAGTGGATGCCGCCTTCGATCTCGGCCTGTTGAAGAGCGCGCGCGCCGCGCGCATC
GCATACTGGATCGAGCGGCTCGTGCTGAAGCGCTTCGACGTGGTCTCATCGATCTCGAACCAGATGGTGCGGCGCGCCGT
CGCCAAGGGCGTCGATGCGGACAAGACCGAGTTTCTGCCGAACTGGGTCGACACGCGCGACATCTTTCCGCTCGGCCGCG
TCAGCGAATATCGTCAGACGCTCGGCATTCCCGCCGACAACACGGTCGTCCTTTACTCCGGCAACATCGGCGCGAAGCAG
GGCATCGAAACGCTCGCCGACGCAGCGGCCGCCCTCGTTGCGCGCAACGACATCAGCTTTGTCTTTTGCGGAAATGGCGC
AGCGCGTGACGATCTCGTAAAGCGCTGCGAGGGCTTGTCCAATTGCCGCTTCCTGCCGTTGCAACCGTCGTCGTCGCTGA
ACGAACTGCTGAACGTTGCGGATATTCACGTGTTGCCGCAGCGCAGCGACGCGGCCGACCTCGTGATGCCGTCGAAGCTG
ACGGGCATGCTGGCGAGCGGCGCTGCGGTCATCGCGATGGCGCGGCCCGGCACCGGGCTCCATGAGGCAGTAGTGAACAA
CGGCGTGGTCGTGCCGCCTGAAAACACCGCTGCGCTCGTCGACGCGATCGCGGCACTCGCTGCCGATCGCGAGCAGCGCG
CCGCGATTGGCGCCGCAGGGCGGCGCTATGCGGAGACGATGCTGTCGCCGCTCTCGACGCTGCTCACGCTCGATACGCGT
CTTGCGCTGCTGACGGGGGGCGATGCGTCGGGTGCCAAGCCGGTCGCTGCACCATTGACGAATGCGCCGATCCTGCCTGC
TCGCGTCGAGGAGTCGGAAGTGGAGTGA

Upstream 100 bases:

>100_bases
CACGCGCGGCCTGAATCACGCAGGTCGGGGCCGCGTCGGTACGGGGAAGGCGTAGCGCTGTCCGCTGCCGCATGACGGAC
ACACGTCGTGGAAGTCAACT

Downstream 100 bases:

>100_bases
CGAGCGCCGGGCACACTGACACCGGAAAGAGAGAAGGCCGCATGCGAACCGTCACGGGACGGTCGCATGCGGCCTTCGGC
TTTTGCATGGCTCAGATTGC

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKILIYGLNYAPELSGAGKYTAEMAQMLVECGHDVRVICAPPYYPDWKVAQGYSMWRYRRDVMRGVHIWRAPLWVPPRPG
GLKRMVHLASFALASLPLLARQIAWRPGAVMLIAPTMACAPGALALARVTGAKAWLHIQDYEVDAAFDLGLLKSARAARI
AYWIERLVLKRFDVVSSISNQMVRRAVAKGVDADKTEFLPNWVDTRDIFPLGRVSEYRQTLGIPADNTVVLYSGNIGAKQ
GIETLADAAAALVARNDISFVFCGNGAARDDLVKRCEGLSNCRFLPLQPSSSLNELLNVADIHVLPQRSDAADLVMPSKL
TGMLASGAAVIAMARPGTGLHEAVVNNGVVVPPENTAALVDAIAALAADREQRAAIGAAGRRYAETMLSPLSTLLTLDTR
LALLTGGDASGAKPVAAPLTNAPILPARVEESEVE

Sequences:

>Translated_435_residues
MKILIYGLNYAPELSGAGKYTAEMAQMLVECGHDVRVICAPPYYPDWKVAQGYSMWRYRRDVMRGVHIWRAPLWVPPRPG
GLKRMVHLASFALASLPLLARQIAWRPGAVMLIAPTMACAPGALALARVTGAKAWLHIQDYEVDAAFDLGLLKSARAARI
AYWIERLVLKRFDVVSSISNQMVRRAVAKGVDADKTEFLPNWVDTRDIFPLGRVSEYRQTLGIPADNTVVLYSGNIGAKQ
GIETLADAAAALVARNDISFVFCGNGAARDDLVKRCEGLSNCRFLPLQPSSSLNELLNVADIHVLPQRSDAADLVMPSKL
TGMLASGAAVIAMARPGTGLHEAVVNNGVVVPPENTAALVDAIAALAADREQRAAIGAAGRRYAETMLSPLSTLLTLDTR
LALLTGGDASGAKPVAAPLTNAPILPARVEESEVE
>Mature_435_residues
MKILIYGLNYAPELSGAGKYTAEMAQMLVECGHDVRVICAPPYYPDWKVAQGYSMWRYRRDVMRGVHIWRAPLWVPPRPG
GLKRMVHLASFALASLPLLARQIAWRPGAVMLIAPTMACAPGALALARVTGAKAWLHIQDYEVDAAFDLGLLKSARAARI
AYWIERLVLKRFDVVSSISNQMVRRAVAKGVDADKTEFLPNWVDTRDIFPLGRVSEYRQTLGIPADNTVVLYSGNIGAKQ
GIETLADAAAALVARNDISFVFCGNGAARDDLVKRCEGLSNCRFLPLQPSSSLNELLNVADIHVLPQRSDAADLVMPSKL
TGMLASGAAVIAMARPGTGLHEAVVNNGVVVPPENTAALVDAIAALAADREQRAAIGAAGRRYAETMLSPLSTLLTLDTR
LALLTGGDASGAKPVAAPLTNAPILPARVEESEVE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=407, Percent_Identity=42.014742014742, Blast_Score=288, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 46753; Mature: 46753

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIYGLNYAPELSGAGKYTAEMAQMLVECGHDVRVICAPPYYPDWKVAQGYSMWRYRR
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHHHHHHH
DVMRGVHIWRAPLWVPPRPGGLKRMVHLASFALASLPLLARQIAWRPGAVMLIAPTMACA
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHCC
PGALALARVTGAKAWLHIQDYEVDAAFDLGLLKSARAARIAYWIERLVLKRFDVVSSISN
CCHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMVRRAVAKGVDADKTEFLPNWVDTRDIFPLGRVSEYRQTLGIPADNTVVLYSGNIGAKQ
HHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHH
GIETLADAAAALVARNDISFVFCGNGAARDDLVKRCEGLSNCRFLPLQPSSSLNELLNVA
HHHHHHHHHHHHHEECCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
DIHVLPQRSDAADLVMPSKLTGMLASGAAVIAMARPGTGLHEAVVNNGVVVPPENTAALV
HEEEECCCCCCCCEECCHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEECCCCHHHHH
DAIAALAADREQRAAIGAAGRRYAETMLSPLSTLLTLDTRLALLTGGDASGAKPVAAPLT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCC
NAPILPARVEESEVE
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKILIYGLNYAPELSGAGKYTAEMAQMLVECGHDVRVICAPPYYPDWKVAQGYSMWRYRR
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHHHHHHH
DVMRGVHIWRAPLWVPPRPGGLKRMVHLASFALASLPLLARQIAWRPGAVMLIAPTMACA
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHCC
PGALALARVTGAKAWLHIQDYEVDAAFDLGLLKSARAARIAYWIERLVLKRFDVVSSISN
CCHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMVRRAVAKGVDADKTEFLPNWVDTRDIFPLGRVSEYRQTLGIPADNTVVLYSGNIGAKQ
HHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHH
GIETLADAAAALVARNDISFVFCGNGAARDDLVKRCEGLSNCRFLPLQPSSSLNELLNVA
HHHHHHHHHHHHHEECCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
DIHVLPQRSDAADLVMPSKLTGMLASGAAVIAMARPGTGLHEAVVNNGVVVPPENTAALV
HEEEECCCCCCCCEECCHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEECCCCHHHHH
DAIAALAADREQRAAIGAAGRRYAETMLSPLSTLLTLDTRLALLTGGDASGAKPVAAPLT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCC
NAPILPARVEESEVE
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]