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The map label for this gene is wcaF [H]

Identifier: 186477215

GI number: 186477215

Start: 2769144

End: 2769857

Strand: Reverse

Name: wcaF [H]

Synonym: Bphy_2466

Alternate gene names: 186477215

Gene position: 2769857-2769144 (Counterclockwise)

Preceding gene: 186477217

Following gene: 186477214

Centisome position: 79.61

GC content: 64.29

Gene sequence:

>714_bases
ATGGATAACACGTTCGGCCCGACCCTTGGGCGGGCAGCGGACGGCCAGGACGCGCGGATGAAAGACCAGCATGCCGCGCG
CGCAACCGAGGCCGCTGTGCCGCCCGATTCGCCCCTGCACGATGCTTCCGGTGCGACCCAGCCGCCCGGCCGCGTGATCG
ACCTGAGCCTCGCGGGCAAGGGCAATTACGTCGCGTCGCGCGGCTTTTTCACCGAGCTGATCTGGTTCTTTGTCGAAGCC
TGCGTGCTCAATAACAAACTGCTGCCCGTGTCGTCGGTGCGCGTCGCGTTGCTGAGGCTGTTCGGCGCGAAGATCGGTGC
GAACTGCCGCTTCGTGCATCCCGTGCGTGTGAAGGCGCCGTGGAACCTCACGGTCGGCGACAACTGCTGGTTCGGCGTCG
ACGTGTGGATCTACAACCAGGCGCCCATCCGCATCGGCTCGAACGTGTGCATCTCGCAAGGCAGCTTTCTGAGCGCGGGT
TCGCATGACATGCGAACCAACATGGATTTGCGCGTGGCGCCGATCGTCATCGAAGACGGCGCGTGGATCTCGTCGAAATG
CGTCGTGCAGATGGGCGTGACGATCGGCCGTTCGGCCGTCGTGACGCCGCTGTCCGTCGTGCACCGTTCACTCGAGGCGG
AAGGTGTGTATGGCGGCAATCCGTGCCGCTTTATCCGCAAGCGTTTTGACGCGGATGGGGGGTGCGTCTCATAA

Upstream 100 bases:

>100_bases
GCGTGAGCCAGATAATCAAACGACAGGGCGCGCAACGTCCGGCCTGGCCGCATAACGGGATGAAGAGCCAGCGATGCGCC
TCCATCGATTGGAGAACACT

Downstream 100 bases:

>100_bases
CATTCGCAGGATGCATGGTTCTGTCTGCCGGAAGCGCGAAGTTGGCGGATGAACTGCATTACGCGGAGCCATCATCCTAC
GTAAGCACTGGCTGGCGCGG

Product: acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MDNTFGPTLGRAADGQDARMKDQHAARATEAAVPPDSPLHDASGATQPPGRVIDLSLAGKGNYVASRGFFTELIWFFVEA
CVLNNKLLPVSSVRVALLRLFGAKIGANCRFVHPVRVKAPWNLTVGDNCWFGVDVWIYNQAPIRIGSNVCISQGSFLSAG
SHDMRTNMDLRVAPIVIEDGAWISSKCVVQMGVTIGRSAVVTPLSVVHRSLEAEGVYGGNPCRFIRKRFDADGGCVS

Sequences:

>Translated_237_residues
MDNTFGPTLGRAADGQDARMKDQHAARATEAAVPPDSPLHDASGATQPPGRVIDLSLAGKGNYVASRGFFTELIWFFVEA
CVLNNKLLPVSSVRVALLRLFGAKIGANCRFVHPVRVKAPWNLTVGDNCWFGVDVWIYNQAPIRIGSNVCISQGSFLSAG
SHDMRTNMDLRVAPIVIEDGAWISSKCVVQMGVTIGRSAVVTPLSVVHRSLEAEGVYGGNPCRFIRKRFDADGGCVS
>Mature_237_residues
MDNTFGPTLGRAADGQDARMKDQHAARATEAAVPPDSPLHDASGATQPPGRVIDLSLAGKGNYVASRGFFTELIWFFVEA
CVLNNKLLPVSSVRVALLRLFGAKIGANCRFVHPVRVKAPWNLTVGDNCWFGVDVWIYNQAPIRIGSNVCISQGSFLSAG
SHDMRTNMDLRVAPIVIEDGAWISSKCVVQMGVTIGRSAVVTPLSVVHRSLEAEGVYGGNPCRFIRKRFDADGGCVS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=160, Percent_Identity=40, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1786537, Length=143, Percent_Identity=31.4685314685315, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786664, Length=150, Percent_Identity=30, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 25500; Mature: 25500

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNTFGPTLGRAADGQDARMKDQHAARATEAAVPPDSPLHDASGATQPPGRVIDLSLAGK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
GNYVASRGFFTELIWFFVEACVLNNKLLPVSSVRVALLRLFGAKIGANCRFVHPVRVKAP
CCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECC
WNLTVGDNCWFGVDVWIYNQAPIRIGSNVCISQGSFLSAGSHDMRTNMDLRVAPIVIEDG
EEEEECCCCEEEEEEEEECCCCEEECCCEEECCCCEECCCCCCHHCCCCEEEEEEEEECC
AWISSKCVVQMGVTIGRSAVVTPLSVVHRSLEAEGVYGGNPCRFIRKRFDADGGCVS
CEECCCEEEEECCEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDNTFGPTLGRAADGQDARMKDQHAARATEAAVPPDSPLHDASGATQPPGRVIDLSLAGK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
GNYVASRGFFTELIWFFVEACVLNNKLLPVSSVRVALLRLFGAKIGANCRFVHPVRVKAP
CCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECC
WNLTVGDNCWFGVDVWIYNQAPIRIGSNVCISQGSFLSAGSHDMRTNMDLRVAPIVIEDG
EEEEECCCCEEEEEEEEECCCCEEECCCEEECCCCEECCCCCCHHCCCCEEEEEEEEECC
AWISSKCVVQMGVTIGRSAVVTPLSVVHRSLEAEGVYGGNPCRFIRKRFDADGGCVS
CEECCCEEEEECCEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]