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The map label for this gene is tolB

Identifier: 186477204

GI number: 186477204

Start: 2753859

End: 2755151

Strand: Direct

Name: tolB

Synonym: Bphy_2455

Alternate gene names: 186477204

Gene position: 2753859-2755151 (Clockwise)

Preceding gene: 186477203

Following gene: 186477205

Centisome position: 79.15

GC content: 64.11

Gene sequence:

>1293_bases
ATGAGTTTGATGACGAAGCTAGGCCTGCGAGCACTCGTAGCGTCGTGTCTGATCGCCGCGGGCGGTGCCGCCCATGCACA
ACTCAACGTCCTCGTGACGGGCGTCGGATCCACGCAGTTCCCGATTGCTACGGCAAATTTTGCCAACGAGGCAAATTCTC
CCCAGCAGATCAGCACCATCGTCCGCCAGGACCTTCAGCGCAGCGGTAAATTCACCAACATCGACGCGGGCAGCGCGCCC
GTATCGGAAGGCGATTCGGTCGATCTCGGCGCATGGAAGGCAAAGGGCGCGAACGCGTTCGTCGCGGGCAGCGTCACGAA
GCTGCCGAACGGCCAGTACCAGGTTCGCTTCAAGCTGTACGACACCGTCAACCAGCAAAGCCTCGGCGGCCTCGAACTGG
TCAGCCCGGAAGGCGGCCTGCGCATGAGCGCGCACAAGGTGGCCGACTACATCTACGCGAAGCTGATGGGCGGCCGCGGC
GTGTTCGCGACGCGTCTGTCGTACGTGATCAAGACGGGCAACCGCTATCAGCTGCAGATTTCCGATTCCGACGGCCAGGA
CGCGCACATCGCACTGTCCAGCCCGGAGCCCATCATCTCGCCGGCGTGGTCGCCGGACGGCACGAAGGTCGCGTACGTCT
CGTTCGAAAAGAAAAAGCCGATCGTCTATATCCACGATCTGCCGACGGGCCGCCGCGTCGTCGTGTCGGACCAGAAGGGC
AACAACAGTGCGCCCGCGTGGTCGCCTGACGGCCGCACGCTCGCCGTCGCACTGTCGCGCACGGGCAACACGCAGATTTT
CGCCGTCAACGCCGACGGCAGCGGCCTACGCCGCCTGACGCAAGGCAGCTCTATCGACACCGAGCCGTCGTACTCGCCCG
ATGGCCAGTCGATCTACTTCACGAGCGACCGCGGCGGCCAGCCGCAGATCTACAAGATGCCCGCCAGCGGCGAGGCATCG
GGCGGCGCACAGCGCGTGACGTTCACGGGCAACTACAATACGAGTCCGCGCGTGAGCCCGGACGGCAAGCAGCTCGCCTA
TATCTCGCGCACGGGCGGGGGCTTCAAGCTGTATATCCAGGATCTGCAGTCGGGCGTCGCGACGGCGCTCACCGATACAA
CACATGACGAATCGCCGAGCTTTGCGGCGAATGGTCAGTACATTCTTTACGCCACTCAGGTGAACGGCCGTGGCGTGTTG
GCCGCAGTATCGACCGACGGTCGGACGCGGCAGGTCCTGTCCGTTCAGGGGGGCAGCGTACGCGAGCCGTCCTGGGGCCC
GTTTATGCAATAA

Upstream 100 bases:

>100_bases
CGCCCGCTCGCGCGGGCCTAGCGGGAACGATTTCTGCTCAAGGCGGTCTGTTTGCAGTTGTGGAGTGTTAGAAATTTGTT
TTTTGGGGAATCTATAAACA

Downstream 100 bases:

>100_bases
CACACAAGGAGAGTAAAGCATGATGTCCAAACTTCGTATCGCGTTGGCCGTGGCAATGGTCGGCACGCTGGCTGCCTGTC
ATTCGGGCGTAAAGCTCGAC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSLMTKLGLRALVASCLIAAGGAAHAQLNVLVTGVGSTQFPIATANFANEANSPQQISTIVRQDLQRSGKFTNIDAGSAP
VSEGDSVDLGAWKAKGANAFVAGSVTKLPNGQYQVRFKLYDTVNQQSLGGLELVSPEGGLRMSAHKVADYIYAKLMGGRG
VFATRLSYVIKTGNRYQLQISDSDGQDAHIALSSPEPIISPAWSPDGTKVAYVSFEKKKPIVYIHDLPTGRRVVVSDQKG
NNSAPAWSPDGRTLAVALSRTGNTQIFAVNADGSGLRRLTQGSSIDTEPSYSPDGQSIYFTSDRGGQPQIYKMPASGEAS
GGAQRVTFTGNYNTSPRVSPDGKQLAYISRTGGGFKLYIQDLQSGVATALTDTTHDESPSFAANGQYILYATQVNGRGVL
AAVSTDGRTRQVLSVQGGSVREPSWGPFMQ

Sequences:

>Translated_430_residues
MSLMTKLGLRALVASCLIAAGGAAHAQLNVLVTGVGSTQFPIATANFANEANSPQQISTIVRQDLQRSGKFTNIDAGSAP
VSEGDSVDLGAWKAKGANAFVAGSVTKLPNGQYQVRFKLYDTVNQQSLGGLELVSPEGGLRMSAHKVADYIYAKLMGGRG
VFATRLSYVIKTGNRYQLQISDSDGQDAHIALSSPEPIISPAWSPDGTKVAYVSFEKKKPIVYIHDLPTGRRVVVSDQKG
NNSAPAWSPDGRTLAVALSRTGNTQIFAVNADGSGLRRLTQGSSIDTEPSYSPDGQSIYFTSDRGGQPQIYKMPASGEAS
GGAQRVTFTGNYNTSPRVSPDGKQLAYISRTGGGFKLYIQDLQSGVATALTDTTHDESPSFAANGQYILYATQVNGRGVL
AAVSTDGRTRQVLSVQGGSVREPSWGPFMQ
>Mature_429_residues
SLMTKLGLRALVASCLIAAGGAAHAQLNVLVTGVGSTQFPIATANFANEANSPQQISTIVRQDLQRSGKFTNIDAGSAPV
SEGDSVDLGAWKAKGANAFVAGSVTKLPNGQYQVRFKLYDTVNQQSLGGLELVSPEGGLRMSAHKVADYIYAKLMGGRGV
FATRLSYVIKTGNRYQLQISDSDGQDAHIALSSPEPIISPAWSPDGTKVAYVSFEKKKPIVYIHDLPTGRRVVVSDQKGN
NSAPAWSPDGRTLAVALSRTGNTQIFAVNADGSGLRRLTQGSSIDTEPSYSPDGQSIYFTSDRGGQPQIYKMPASGEASG
GAQRVTFTGNYNTSPRVSPDGKQLAYISRTGGGFKLYIQDLQSGVATALTDTTHDESPSFAANGQYILYATQVNGRGVLA
AVSTDGRTRQVLSVQGGSVREPSWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=450, Percent_Identity=36.8888888888889, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_BURP8 (B2JGA0)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858674.1
- ProteinModelPortal:   B2JGA0
- SMR:   B2JGA0
- GeneID:   6243939
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2455
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK02889
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 45467; Mature: 45336

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMTKLGLRALVASCLIAAGGAAHAQLNVLVTGVGSTQFPIATANFANEANSPQQISTI
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
VRQDLQRSGKFTNIDAGSAPVSEGDSVDLGAWKAKGANAFVAGSVTKLPNGQYQVRFKLY
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCEECCCCCEEEECCEEECCCCCEEEEEEEE
DTVNQQSLGGLELVSPEGGLRMSAHKVADYIYAKLMGGRGVFATRLSYVIKTGNRYQLQI
ECCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCEEEEEE
SDSDGQDAHIALSSPEPIISPAWSPDGTKVAYVSFEKKKPIVYIHDLPTGRRVVVSDQKG
ECCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDGRTLAVALSRTGNTQIFAVNADGSGLRRLTQGSSIDTEPSYSPDGQSIYF
CCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEE
TSDRGGQPQIYKMPASGEASGGAQRVTFTGNYNTSPRVSPDGKQLAYISRTGGGFKLYIQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
DLQSGVATALTDTTHDESPSFAANGQYILYATQVNGRGVLAAVSTDGRTRQVLSVQGGSV
HHHCCHHEEEECCCCCCCCCEECCCEEEEEEEEECCCEEEEEEECCCCEEEEEEECCCCC
REPSWGPFMQ
CCCCCCCCCC
>Mature Secondary Structure 
SLMTKLGLRALVASCLIAAGGAAHAQLNVLVTGVGSTQFPIATANFANEANSPQQISTI
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
VRQDLQRSGKFTNIDAGSAPVSEGDSVDLGAWKAKGANAFVAGSVTKLPNGQYQVRFKLY
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCEECCCCCEEEECCEEECCCCCEEEEEEEE
DTVNQQSLGGLELVSPEGGLRMSAHKVADYIYAKLMGGRGVFATRLSYVIKTGNRYQLQI
ECCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCEEEEEE
SDSDGQDAHIALSSPEPIISPAWSPDGTKVAYVSFEKKKPIVYIHDLPTGRRVVVSDQKG
ECCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDGRTLAVALSRTGNTQIFAVNADGSGLRRLTQGSSIDTEPSYSPDGQSIYF
CCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEE
TSDRGGQPQIYKMPASGEASGGAQRVTFTGNYNTSPRVSPDGKQLAYISRTGGGFKLYIQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
DLQSGVATALTDTTHDESPSFAANGQYILYATQVNGRGVLAAVSTDGRTRQVLSVQGGSV
HHHCCHHEEEECCCCCCCCCEECCCEEEEEEEEECCCEEEEEEECCCCEEEEEEECCCCC
REPSWGPFMQ
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA