BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

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The map label for this gene is ydfG [H]

Identifier: 186477199

GI number: 186477199

Start: 2750041

End: 2750787

Strand: Direct

Name: ydfG [H]

Synonym: Bphy_2450

Alternate gene names: 186477199

Gene position: 2750041-2750787 (Clockwise)

Preceding gene: 186477191

Following gene: 186477200

Centisome position: 79.04

GC content: 66.93

Gene sequence:

>747_bases
ATGATCGTGTTCGTCACCGGGGCGTCCGCAGGATTCGGCGCCGCCATCGCCCGGGCTTTCGTCAAAGGCGGCCATCGCGT
CGTCGCAACCGCGCGCCGCAAGGACCGCCTTCAGGCGCTCGCTGACGAACTCGGGGACAACCTGCTGCCGTTCGAACTCG
ACGTGCGCGACCGCGCGGCTGTCCAAGCCCTCCCCGCCGCGCTGCCCGCCGGCTTTGCCGCCGTCGACGTGCTCGTCAAC
AATGCCGGCCTCGCGCTCGGTCTCGAGCCCGCGCAACGCGCCGAGCTCGACGACTGGACCACGATGATCGATACCAACTG
CACGGGTCTCGTGCAGGTCACGCGCGCGTTCTTGCCCGGCATGATCGAGCGCAAGCGCGGACATGTGTTCAATCTGGGCT
CGGTGGCGGGCACGTGGCCCTATCCGGGCGGCAACGTGTATGGCGCGACCAAGGCGTTCGTGCGCCAGTTCAGTCTGAAC
CTGCGCGCGGACCTCCACGGCACGCCCATTCGCGTGACGGACATCGAGCCGGGACTGTGCGGCGGCACCGAATTTTCCAA
CGTCCGTTTCCGCGGCGACGAGGAAAAAGCTGCGACCGTCTACCAGAACGTCCAGCCGCTCACGGCGGAAGACATCGCCG
ACTCCATCTACTGGATCGCGACGCGCCCCGCGCACGTCAACATCAATACGATCGAGCTGATGCCCGTCGGACAGTCGTTC
GCCGGCCTGAACATCCACCGGGGTTAG

Upstream 100 bases:

>100_bases
CGGCCTGCCCGGACGCACCGTCGCGCGGCGGAGTCATTCTTGCCGCATCGTGCCATTGGAAGTAGGCTTGTGGCCTTTCT
CCACCGACCAGGGAACAGCA

Downstream 100 bases:

>100_bases
TATCGCGAACCCGGCCCGGATCGGGCCGCCTCGTGCGGCCTCGGTGCGGCGCCTGTCGCGTGCGCAGAACACATTCCGGT
ACAACAGTGCCCAAAAATGC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQALADELGDNLLPFELDVRDRAAVQALPAALPAGFAAVDVLVN
NAGLALGLEPAQRAELDDWTTMIDTNCTGLVQVTRAFLPGMIERKRGHVFNLGSVAGTWPYPGGNVYGATKAFVRQFSLN
LRADLHGTPIRVTDIEPGLCGGTEFSNVRFRGDEEKAATVYQNVQPLTAEDIADSIYWIATRPAHVNINTIELMPVGQSF
AGLNIHRG

Sequences:

>Translated_248_residues
MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQALADELGDNLLPFELDVRDRAAVQALPAALPAGFAAVDVLVN
NAGLALGLEPAQRAELDDWTTMIDTNCTGLVQVTRAFLPGMIERKRGHVFNLGSVAGTWPYPGGNVYGATKAFVRQFSLN
LRADLHGTPIRVTDIEPGLCGGTEFSNVRFRGDEEKAATVYQNVQPLTAEDIADSIYWIATRPAHVNINTIELMPVGQSF
AGLNIHRG
>Mature_248_residues
MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQALADELGDNLLPFELDVRDRAAVQALPAALPAGFAAVDVLVN
NAGLALGLEPAQRAELDDWTTMIDTNCTGLVQVTRAFLPGMIERKRGHVFNLGSVAGTWPYPGGNVYGATKAFVRQFSLN
LRADLHGTPIRVTDIEPGLCGGTEFSNVRFRGDEEKAATVYQNVQPLTAEDIADSIYWIATRPAHVNINTIELMPVGQSF
AGLNIHRG

Specific function: Catalyzes the NADP-dependent oxidation of L-allo- threonine and L-serine [H]

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=33.0645161290323, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI7706318, Length=255, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI20149619, Length=193, Percent_Identity=33.160621761658, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI7705855, Length=194, Percent_Identity=29.3814432989691, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI22507405, Length=178, Percent_Identity=33.7078651685393, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI150247226, Length=192, Percent_Identity=34.8958333333333, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI40548397, Length=206, Percent_Identity=31.5533980582524, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI214010158, Length=206, Percent_Identity=31.5533980582524, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI214010156, Length=206, Percent_Identity=31.5533980582524, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI22758144, Length=206, Percent_Identity=31.5533980582524, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI7657478, Length=184, Percent_Identity=35.3260869565217, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI157739938, Length=184, Percent_Identity=28.2608695652174, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI40254992, Length=179, Percent_Identity=31.2849162011173, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI19923817, Length=186, Percent_Identity=27.4193548387097, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4557649, Length=178, Percent_Identity=28.6516853932584, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI19743808, Length=178, Percent_Identity=29.7752808988764, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI44680136, Length=184, Percent_Identity=29.8913043478261, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI44680133, Length=184, Percent_Identity=29.8913043478261, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI17738292, Length=184, Percent_Identity=29.8913043478261, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4758504, Length=192, Percent_Identity=30.7291666666667, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI10190704, Length=228, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI223718074, Length=160, Percent_Identity=37.5, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI32483357, Length=210, Percent_Identity=27.6190476190476, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787820, Length=247, Percent_Identity=66.8016194331984, Blast_Score=366, Evalue=1e-103,
Organism=Escherichia coli, GI1787335, Length=238, Percent_Identity=31.0924369747899, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082100, Length=179, Percent_Identity=35.195530726257, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI87082160, Length=179, Percent_Identity=30.1675977653631, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786812, Length=179, Percent_Identity=29.608938547486, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786701, Length=181, Percent_Identity=28.7292817679558, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=239, Percent_Identity=26.3598326359833, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=26.4, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=259, Percent_Identity=25.8687258687259, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=189, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=269, Percent_Identity=25.6505576208178, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534660, Length=180, Percent_Identity=25, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=184, Percent_Identity=28.2608695652174, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=188, Percent_Identity=28.1914893617021, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=242, Percent_Identity=26.8595041322314, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=260, Percent_Identity=25.3846153846154, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=169, Percent_Identity=30.1775147928994, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=207, Percent_Identity=27.0531400966184, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=169, Percent_Identity=30.1775147928994, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71982365, Length=140, Percent_Identity=33.5714285714286, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=246, Percent_Identity=37.8048780487805, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322067, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322227, Length=199, Percent_Identity=25.1256281407035, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI116007236, Length=253, Percent_Identity=33.201581027668, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281364494, Length=252, Percent_Identity=32.1428571428571, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24663911, Length=252, Percent_Identity=29.7619047619048, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571526, Length=236, Percent_Identity=29.6610169491525, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI116007234, Length=253, Percent_Identity=33.201581027668, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21358495, Length=184, Percent_Identity=31.5217391304348, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI116007238, Length=186, Percent_Identity=34.9462365591398, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24641388, Length=252, Percent_Identity=31.7460317460317, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21357041, Length=236, Percent_Identity=28.3898305084746, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641232, Length=255, Percent_Identity=29.8039215686275, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI23397609, Length=242, Percent_Identity=29.7520661157025, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640442, Length=193, Percent_Identity=30.0518134715026, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648693, Length=251, Percent_Identity=31.0756972111554, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355889, Length=251, Percent_Identity=31.0756972111554, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24643142, Length=176, Percent_Identity=31.25, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28571387, Length=254, Percent_Identity=29.9212598425197, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24584748, Length=180, Percent_Identity=29.4444444444444, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI161076933, Length=180, Percent_Identity=29.4444444444444, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28574402, Length=185, Percent_Identity=29.7297297297297, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24584744, Length=185, Percent_Identity=29.7297297297297, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644339, Length=236, Percent_Identity=26.271186440678, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648944, Length=241, Percent_Identity=28.2157676348548, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355631, Length=170, Percent_Identity=28.2352941176471, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584752, Length=185, Percent_Identity=28.6486486486486, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364892, Length=192, Percent_Identity=31.25, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007232, Length=186, Percent_Identity=35.4838709677419, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580925, Length=233, Percent_Identity=25.3218884120172, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24639444, Length=244, Percent_Identity=29.9180327868852, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26581; Mature: 26581

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQALADELGDNLLPFELDVRDRAA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEECCCHHHH
VQALPAALPAGFAAVDVLVNNAGLALGLEPAQRAELDDWTTMIDTNCTGLVQVTRAFLPG
HHHHHHHHCCCHHEEEEEECCCCEEEECCCHHHCCCCCHHEEECCCCHHHHHHHHHHHCH
MIERKRGHVFNLGSVAGTWPYPGGNVYGATKAFVRQFSLNLRADLHGTPIRVTDIEPGLC
HHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCEEEEECCCCCC
GGTEFSNVRFRGDEEKAATVYQNVQPLTAEDIADSIYWIATRPAHVNINTIELMPVGQSF
CCCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHCCEEEEEECCCEEEEEEEEEEECCCCC
AGLNIHRG
CCCEEECC
>Mature Secondary Structure
MIVFVTGASAGFGAAIARAFVKGGHRVVATARRKDRLQALADELGDNLLPFELDVRDRAA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEECCCHHHH
VQALPAALPAGFAAVDVLVNNAGLALGLEPAQRAELDDWTTMIDTNCTGLVQVTRAFLPG
HHHHHHHHCCCHHEEEEEECCCCEEEECCCHHHCCCCCHHEEECCCCHHHHHHHHHHHCH
MIERKRGHVFNLGSVAGTWPYPGGNVYGATKAFVRQFSLNLRADLHGTPIRVTDIEPGLC
HHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCEEEEECCCCCC
GGTEFSNVRFRGDEEKAATVYQNVQPLTAEDIADSIYWIATRPAHVNINTIELMPVGQSF
CCCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHCCEEEEEECCCEEEEEEEEEEECCCCC
AGLNIHRG
CCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]