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The map label for this gene is oprM [H]

Identifier: 186477160

GI number: 186477160

Start: 2707542

End: 2708993

Strand: Direct

Name: oprM [H]

Synonym: Bphy_2409

Alternate gene names: 186477160

Gene position: 2707542-2708993 (Clockwise)

Preceding gene: 186477159

Following gene: 186477161

Centisome position: 77.82

GC content: 67.36

Gene sequence:

>1452_bases
ATGCTGCTGTCGGGCTGCACGGTCGGCCCCGATTACCGCACGCCTGCCCCGCCCGCCACCCAAACCTACACGCCCGCGCC
GTTGCCCGACGAGACGGCCCCGTCGCCGGGCGCAACGGGCGTGCGGCAGCAGTTCGTCACCGGACAGGACATCCCCGCGC
AATGGTGGACGCTCTTTCATTGCGAGCCGCTCGACGCGCTGATCCGGGAAGCGCTCGCGAACAGCCCGAACGTCGCCGCT
GCGCAAGCTGCATTGCGTCAGGCGGCGGAGAACTATCGCGCGGAAGTGGGCAGCGCGCTCTATCCGTCGGTGGATGCGAA
GCTCAATGCGACACGCGAGAAGTTCAACGGCGTCACGTTCGGCCAGCCCGGGCTCACGGAAGTGCTGAATCTGTATAACG
CGTCGGTGAACGTCTCGTACAACCTCGACGTGTTCGGCGGCTCGCGGCGCGAACTGGAATCGCTGCGCTCACAGATCGAT
TACCAGGGCTATCAGTTGCAGGCCGCGTATCTCGCGCTGTCGGCAAACATCGTTACGGCGGCCGTCAAGGAAGCGTCGCT
GCGCGAGCAGATCGATGCAACGGAACGCATCGCCCGCGACGAGGCATCGCAGCTCGACGTCTTGCACAAGCAGTTCGATC
TGGGCGGCGTGGGCCGCACTGCCGTACTCTCGCAGCAAACGTTGCTCGCGCAAACACGCGCGAGCTTGCCGCCGCTGCGT
CAATCGCTCGACCAGACGCGGCATCAGCTCGCGGTGCTCGCAGGCAAGCCGCCCAGCGATGCGGGCGTGCCCGAATTCAG
GCTGTCGATGTTCACGCTGCCGCAAAGCCTGCCCGTGAGTCTGCCTTCATCGCTCGTCAGGCAGCGCCCCGACATTCTCG
CCGCCGACGCGACGCTGCATCAGGCAAGCGCCCAGGTCGGCGTCGCGACGGCGAACATGTATCCGCAGATCACGCTGTCC
GCGAGTTACGGTCCGCAGGCGCTGACGCCCGCCGGCCTGCTCAAGTATGCCGACATGATCTGGAGCATCGGTGCGGGCAT
CACGCAGCCGATCTTCCACGGCGGCCAGTTGAGCGCGCAAAAGCGCGCCGCTGAAGCCGCCTTCGATCAGGCCAACGCGC
AGTACCGCCAGACGGTGCTGCTCGCGTTCCAGAACGTCGCCGATACGCTGCGCGCGCTCGAACACGACGCGACGGGCCTC
GCCGCGCAAACGGACGCATGGCGCTCCGCGAGCGACACGCTCGATCTGACGCGCGGCCAGTTCCGTATCGGCGGGGTCAG
TTATCTGGCGCTGCTCGATGCGCAGCGCCAGTACCAGCAGACTGTCGTGAATCTCGCGCAGGCTCAGGCCGCCCGCTACG
CGGACACGGCGGCGCTGTTCCAGGCGCTCGGCGGCGGCTGGTGGAACGACACGGCGACGGGCACGGCACCGCCCGTCGCC
GCATCGCAGTGA

Upstream 100 bases:

>100_bases
CGCTTCGATCCCTGCTTCGATCCCTGCTTCGATCCCTGCTTCGATCATTGCTTCGATCATTGCTTCGATCACGGCCTTCG
CGATCCTGAGTGCGGCCCTC

Downstream 100 bases:

>100_bases
GCCCGCAGGTTCCTTCAGGTAGTCACCGGGCAGCGCAGGCGACGAAACGCAGCGCGCCGGACAGGTTTGTGCCTCAGTTT
GCCGTGCAAGGACATGACGA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLLSGCTVGPDYRTPAPPATQTYTPAPLPDETAPSPGATGVRQQFVTGQDIPAQWWTLFHCEPLDALIREALANSPNVAA
AQAALRQAAENYRAEVGSALYPSVDAKLNATREKFNGVTFGQPGLTEVLNLYNASVNVSYNLDVFGGSRRELESLRSQID
YQGYQLQAAYLALSANIVTAAVKEASLREQIDATERIARDEASQLDVLHKQFDLGGVGRTAVLSQQTLLAQTRASLPPLR
QSLDQTRHQLAVLAGKPPSDAGVPEFRLSMFTLPQSLPVSLPSSLVRQRPDILAADATLHQASAQVGVATANMYPQITLS
ASYGPQALTPAGLLKYADMIWSIGAGITQPIFHGGQLSAQKRAAEAAFDQANAQYRQTVLLAFQNVADTLRALEHDATGL
AAQTDAWRSASDTLDLTRGQFRIGGVSYLALLDAQRQYQQTVVNLAQAQAARYADTAALFQALGGGWWNDTATGTAPPVA
ASQ

Sequences:

>Translated_483_residues
MLLSGCTVGPDYRTPAPPATQTYTPAPLPDETAPSPGATGVRQQFVTGQDIPAQWWTLFHCEPLDALIREALANSPNVAA
AQAALRQAAENYRAEVGSALYPSVDAKLNATREKFNGVTFGQPGLTEVLNLYNASVNVSYNLDVFGGSRRELESLRSQID
YQGYQLQAAYLALSANIVTAAVKEASLREQIDATERIARDEASQLDVLHKQFDLGGVGRTAVLSQQTLLAQTRASLPPLR
QSLDQTRHQLAVLAGKPPSDAGVPEFRLSMFTLPQSLPVSLPSSLVRQRPDILAADATLHQASAQVGVATANMYPQITLS
ASYGPQALTPAGLLKYADMIWSIGAGITQPIFHGGQLSAQKRAAEAAFDQANAQYRQTVLLAFQNVADTLRALEHDATGL
AAQTDAWRSASDTLDLTRGQFRIGGVSYLALLDAQRQYQQTVVNLAQAQAARYADTAALFQALGGGWWNDTATGTAPPVA
ASQ
>Mature_483_residues
MLLSGCTVGPDYRTPAPPATQTYTPAPLPDETAPSPGATGVRQQFVTGQDIPAQWWTLFHCEPLDALIREALANSPNVAA
AQAALRQAAENYRAEVGSALYPSVDAKLNATREKFNGVTFGQPGLTEVLNLYNASVNVSYNLDVFGGSRRELESLRSQID
YQGYQLQAAYLALSANIVTAAVKEASLREQIDATERIARDEASQLDVLHKQFDLGGVGRTAVLSQQTLLAQTRASLPPLR
QSLDQTRHQLAVLAGKPPSDAGVPEFRLSMFTLPQSLPVSLPSSLVRQRPDILAADATLHQASAQVGVATANMYPQITLS
ASYGPQALTPAGLLKYADMIWSIGAGITQPIFHGGQLSAQKRAAEAAFDQANAQYRQTVLLAFQNVADTLRALEHDATGL
AAQTDAWRSASDTLDLTRGQFRIGGVSYLALLDAQRQYQQTVVNLAQAQAARYADTAALFQALGGGWWNDTATGTAPPVA
ASQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=467, Percent_Identity=26.5524625267666, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI2367348, Length=443, Percent_Identity=24.6049661399549, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51628; Mature: 51628

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSGCTVGPDYRTPAPPATQTYTPAPLPDETAPSPGATGVRQQFVTGQDIPAQWWTLFH
CEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEE
CEPLDALIREALANSPNVAAAQAALRQAAENYRAEVGSALYPSVDAKLNATREKFNGVTF
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
GQPGLTEVLNLYNASVNVSYNLDVFGGSRRELESLRSQIDYQGYQLQAAYLALSANIVTA
CCCCHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
AVKEASLREQIDATERIARDEASQLDVLHKQFDLGGVGRTAVLSQQTLLAQTRASLPPLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
QSLDQTRHQLAVLAGKPPSDAGVPEFRLSMFTLPQSLPVSLPSSLVRQRPDILAADATLH
HHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEECHHHH
QASAQVGVATANMYPQITLSASYGPQALTPAGLLKYADMIWSIGAGITQPIFHGGQLSAQ
HHHHHHEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHH
KRAAEAAFDQANAQYRQTVLLAFQNVADTLRALEHDATGLAAQTDAWRSASDTLDLTRGQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHCCCE
FRIGGVSYLALLDAQRQYQQTVVNLAQAQAARYADTAALFQALGGGWWNDTATGTAPPVA
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ASQ
CCC
>Mature Secondary Structure
MLLSGCTVGPDYRTPAPPATQTYTPAPLPDETAPSPGATGVRQQFVTGQDIPAQWWTLFH
CEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEE
CEPLDALIREALANSPNVAAAQAALRQAAENYRAEVGSALYPSVDAKLNATREKFNGVTF
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
GQPGLTEVLNLYNASVNVSYNLDVFGGSRRELESLRSQIDYQGYQLQAAYLALSANIVTA
CCCCHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
AVKEASLREQIDATERIARDEASQLDVLHKQFDLGGVGRTAVLSQQTLLAQTRASLPPLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
QSLDQTRHQLAVLAGKPPSDAGVPEFRLSMFTLPQSLPVSLPSSLVRQRPDILAADATLH
HHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEECHHHH
QASAQVGVATANMYPQITLSASYGPQALTPAGLLKYADMIWSIGAGITQPIFHGGQLSAQ
HHHHHHEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHH
KRAAEAAFDQANAQYRQTVLLAFQNVADTLRALEHDATGLAAQTDAWRSASDTLDLTRGQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHCCCE
FRIGGVSYLALLDAQRQYQQTVVNLAQAQAARYADTAALFQALGGGWWNDTATGTAPPVA
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ASQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]