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The map label for this gene is gcvA [H]

Identifier: 186477136

GI number: 186477136

Start: 2680902

End: 2681807

Strand: Reverse

Name: gcvA [H]

Synonym: Bphy_2385

Alternate gene names: 186477136

Gene position: 2681807-2680902 (Counterclockwise)

Preceding gene: 186477137

Following gene: 186477135

Centisome position: 77.08

GC content: 59.93

Gene sequence:

>906_bases
ATGAAACGACTCCCGCCGCTCAATTGGTTACGCGCTTTCGAATCATCGGCCCGGCACCTCAGTTTCACACATGCCGCGAC
CGAGCTGAGCCTTACCCAGGCGGCCGTTAGCCAGCAGGTGAAGGGACTCGAATCTCAGCTTGGAACAGCGTTGTTCAAGC
GCCTGCCGCGCGGCATCGAGTTGACCGAGGCCGGGCTTGCGTATCTGCCAGTTGTCCACGAAGCGATCGAGCGACTTGCT
GCTGCCACGGACGAGATCTTCGGGCAAGGACACCTGAAAATGCTGACCGTGCGCGTGAGTCTTGTGTTTTTCACGCACTG
GCTCTCATGGCGGCTGCCCGATTTCCGGAAGCGGTATCCAGACGTTAATCTGCGCATCACCAGCAATATCTGGGGAGGCG
ACAGCAGCGTAACCGATGTCGAAGCCGATCTGGAGATCCGCTACGGCCACGGGAAATGGACTGGCCTGCGTGCGGAGCGA
CTGACGTGGGATACGCTGCTGCCCGTCTGCGCGCCCGGCTTGCCTTCTCCAAAAGCGCCTCTGTCTACGCCCAAGGATCT
CGCGCATCACGAACTGCTCCACGTGCTCGGCTACGAAGAGGGCTGGGGATACTGGCTAAAAAAGGCTGGTGCTTCGAAGG
TCGATTCGTCGAAGGGTATGCAATTCGACACGCTTATCTCCGCACTCAGGGCGGCTGAACTTGGCCAGGGCGTCGCACTC
GCGCGTTCGTCGCTCGTCGAGCACATGCTGATCTCCGGACAACTGATAGCCCCACTGAAGCGTCGGCTTCCAACCAGTGA
GGCGTTCTATCTCGTCTACTCCCCGCACAGCATCGTTAACCCCAATGCGGCTGCGTTCGTCGCATGGTTGAGTGCACAGG
CCGCAGAAGCGCACGCAAGGTACTGA

Upstream 100 bases:

>100_bases
CTTGAAGACGCGCCTCGCCGACGGGTGATTCAGAATGAATCGGCGCAATAGAATGCGAGAATCCCCAATTGTCCGCCCGA
TCCGCTAAATTATTCTCAAG

Downstream 100 bases:

>100_bases
CCTTCGGTCCGCCTGGCACACCCGCATTAGTTCATCGGTGCACGGCGTCCTGCCGTCCGACTCGTCTGCTTGAGCCATAA
AAAAAACCGACCCACCATCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKRLPPLNWLRAFESSARHLSFTHAATELSLTQAAVSQQVKGLESQLGTALFKRLPRGIELTEAGLAYLPVVHEAIERLA
AATDEIFGQGHLKMLTVRVSLVFFTHWLSWRLPDFRKRYPDVNLRITSNIWGGDSSVTDVEADLEIRYGHGKWTGLRAER
LTWDTLLPVCAPGLPSPKAPLSTPKDLAHHELLHVLGYEEGWGYWLKKAGASKVDSSKGMQFDTLISALRAAELGQGVAL
ARSSLVEHMLISGQLIAPLKRRLPTSEAFYLVYSPHSIVNPNAAAFVAWLSAQAAEAHARY

Sequences:

>Translated_301_residues
MKRLPPLNWLRAFESSARHLSFTHAATELSLTQAAVSQQVKGLESQLGTALFKRLPRGIELTEAGLAYLPVVHEAIERLA
AATDEIFGQGHLKMLTVRVSLVFFTHWLSWRLPDFRKRYPDVNLRITSNIWGGDSSVTDVEADLEIRYGHGKWTGLRAER
LTWDTLLPVCAPGLPSPKAPLSTPKDLAHHELLHVLGYEEGWGYWLKKAGASKVDSSKGMQFDTLISALRAAELGQGVAL
ARSSLVEHMLISGQLIAPLKRRLPTSEAFYLVYSPHSIVNPNAAAFVAWLSAQAAEAHARY
>Mature_301_residues
MKRLPPLNWLRAFESSARHLSFTHAATELSLTQAAVSQQVKGLESQLGTALFKRLPRGIELTEAGLAYLPVVHEAIERLA
AATDEIFGQGHLKMLTVRVSLVFFTHWLSWRLPDFRKRYPDVNLRITSNIWGGDSSVTDVEADLEIRYGHGKWTGLRAER
LTWDTLLPVCAPGLPSPKAPLSTPKDLAHHELLHVLGYEEGWGYWLKKAGASKVDSSKGMQFDTLISALRAAELGQGVAL
ARSSLVEHMLISGQLIAPLKRRLPTSEAFYLVYSPHSIVNPNAAAFVAWLSAQAAEAHARY

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=40.6666666666667, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=31.4189189189189, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=30.1038062283737, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=26.3513513513513, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=24.1496598639456, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=25, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33321; Mature: 33321

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50931 HTH_LYSR ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLPPLNWLRAFESSARHLSFTHAATELSLTQAAVSQQVKGLESQLGTALFKRLPRGIE
CCCCCCHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LTEAGLAYLPVVHEAIERLAAATDEIFGQGHLKMLTVRVSLVFFTHWLSWRLPDFRKRYP
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
DVNLRITSNIWGGDSSVTDVEADLEIRYGHGKWTGLRAERLTWDTLLPVCAPGLPSPKAP
CCEEEEECCCCCCCCCCEEEEECEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
LSTPKDLAHHELLHVLGYEEGWGYWLKKAGASKVDSSKGMQFDTLISALRAAELGQGVAL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
ARSSLVEHMLISGQLIAPLKRRLPTSEAFYLVYSPHSIVNPNAAAFVAWLSAQAAEAHAR
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
Y
C
>Mature Secondary Structure
MKRLPPLNWLRAFESSARHLSFTHAATELSLTQAAVSQQVKGLESQLGTALFKRLPRGIE
CCCCCCHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LTEAGLAYLPVVHEAIERLAAATDEIFGQGHLKMLTVRVSLVFFTHWLSWRLPDFRKRYP
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
DVNLRITSNIWGGDSSVTDVEADLEIRYGHGKWTGLRAERLTWDTLLPVCAPGLPSPKAP
CCEEEEECCCCCCCCCCEEEEECEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
LSTPKDLAHHELLHVLGYEEGWGYWLKKAGASKVDSSKGMQFDTLISALRAAELGQGVAL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
ARSSLVEHMLISGQLIAPLKRRLPTSEAFYLVYSPHSIVNPNAAAFVAWLSAQAAEAHAR
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]