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The map label for this gene is nasA [H]

Identifier: 186477088

GI number: 186477088

Start: 2621040

End: 2622437

Strand: Reverse

Name: nasA [H]

Synonym: Bphy_2337

Alternate gene names: 186477088

Gene position: 2622437-2621040 (Counterclockwise)

Preceding gene: 186477089

Following gene: 186477087

Centisome position: 75.37

GC content: 65.59

Gene sequence:

>1398_bases
ATGAAAAACCTGTTGAATTCGCTTGCGAGCGGTAACTGGCGCGCGCTGCTCGCGTGCTTCCTTTACTTCGATACCGGCTT
CACCGTCTGGGTCATGTTCGGGCCGCTGGCGCCGTTCATCAACAAGAGTATCGCGATGTCGCCCGCGGAACTGGGCTTTC
TCGTCGCGGTGCCCGTGCTCGGCGCGGCAATCCTGCGCGTGACGCTTGGCAATCTCTATCAGGCTTGCGACGGTCGACGC
GTGGCGCTGATGGGCGTCGCGCTGTCAGCGATTCCTTCGATCGTGCTGCTGCTGATGCCAGGCGCGCCGTCATACACGCT
GCTGCTGATTCTCGGCGTGTTTCTCGGCGTCGGCGGGGCGAGCTTCGCGGTCGCGTTGCCGATGGCAGGCAGCAACTATC
CGCCGAAAGTGCAAGGCCTGGTGCTCGGCCTCGCGGCCGCGGGCAACATCGGCGCGGTGCTGGACGGCTTCATGTTCCCG
GCGCTTGCCGATCATTTCGGCTGGGCGAAGGCTGCGGGCGCCGCGCTGCCGCTGCTGGGCCTCGCTGCCGTCGCGCTGTT
CTTCTGGGCCAGGGATCTCGGGCAGAAAACGGGCAGCGGCGCACAGGCGCTGCGCAGCTTCATCGTGACGCTCGCGGGTC
TCGTTGCGCTCGTCGTCGCCGTGCATGCGGGCGTGTTCGGCGCGGGCAAGACGGGTGTGCTGCTGCTGCCCGTGCTCGGC
GCGCTGCTCGCGATTGCCGTGCTGCCGAAGCACTATCGCAGCGTGCTCGTCGAAGGCGACACGTGGGTCGTGATGCTGGT
CTACAGCATTACGTTCGGCGGCTTTGTCGGCATGTCCTCGTATGTGACGACACTGCTGATCTCGCTGTATCAGATGCCGC
GTCTCGAAGCCGGACTCTTCATGTCGCTGCTCGCGTGTATCGGTGCGCTTGTGCGTCCCATCGGCGGGCTGGTCGCGGAT
CGCATTTCGGGTGTGCGTGCGCTGGTGATTCTGCTCGCGGCGATCTCGCTGTGCGACTTCCTGTTCGCTGCATGGATGCC
GCCCGTTTCCGCTGGCATCGCCTTGCTGATCGCGATGTATGTGTGCTTCGGTCTCGGCAACGGCGCGACGTTCCAGCTCG
TGCCGCAGCGCTGGAAGGGCAAGACGGGCCTGATGTCGGGCATCGTCGGCGCGGCGGGCGGTATTGGCGGGTTCTATCTG
CCCGTGATCATGGGCATCGCGAAGGAAGGCACGGGCAGCTATCAGATGGGCTTCGCGACGTTCGGCGTGCTGTCGGCGCT
CGCGTTCGGTCTGGTCGTGCTGCATCGCGCGCGCTGGCTCGAGTGGGCGCTGCCGAAGGAAAGTGCGGTGGTGATCGAGC
CGATCCGCGCGGGCATCAGCGTGGATAGCGGAGCTTGA

Upstream 100 bases:

>100_bases
TCGCGTTCTCGCGTCAACACGCGCAGTCCCCAACGCATTTGCACCTAGTTGTTTGCCCGACCCCCGCACCAGCCCTGCTT
TCGTTCAATGAGAGAGACCG

Downstream 100 bases:

>100_bases
CGTCCCCGGTTGTGCGCGTTGTCGATAATCGCCAGCACCTCGCGTGCTGGCGATTTTTTTTCTGGGGCGTGCGGATAAGT
GGCGTGGATGTCATCCACCC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKNLLNSLASGNWRALLACFLYFDTGFTVWVMFGPLAPFINKSIAMSPAELGFLVAVPVLGAAILRVTLGNLYQACDGRR
VALMGVALSAIPSIVLLLMPGAPSYTLLLILGVFLGVGGASFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFMFP
ALADHFGWAKAAGAALPLLGLAAVALFFWARDLGQKTGSGAQALRSFIVTLAGLVALVVAVHAGVFGAGKTGVLLLPVLG
ALLAIAVLPKHYRSVLVEGDTWVVMLVYSITFGGFVGMSSYVTTLLISLYQMPRLEAGLFMSLLACIGALVRPIGGLVAD
RISGVRALVILLAAISLCDFLFAAWMPPVSAGIALLIAMYVCFGLGNGATFQLVPQRWKGKTGLMSGIVGAAGGIGGFYL
PVIMGIAKEGTGSYQMGFATFGVLSALAFGLVVLHRARWLEWALPKESAVVIEPIRAGISVDSGA

Sequences:

>Translated_465_residues
MKNLLNSLASGNWRALLACFLYFDTGFTVWVMFGPLAPFINKSIAMSPAELGFLVAVPVLGAAILRVTLGNLYQACDGRR
VALMGVALSAIPSIVLLLMPGAPSYTLLLILGVFLGVGGASFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFMFP
ALADHFGWAKAAGAALPLLGLAAVALFFWARDLGQKTGSGAQALRSFIVTLAGLVALVVAVHAGVFGAGKTGVLLLPVLG
ALLAIAVLPKHYRSVLVEGDTWVVMLVYSITFGGFVGMSSYVTTLLISLYQMPRLEAGLFMSLLACIGALVRPIGGLVAD
RISGVRALVILLAAISLCDFLFAAWMPPVSAGIALLIAMYVCFGLGNGATFQLVPQRWKGKTGLMSGIVGAAGGIGGFYL
PVIMGIAKEGTGSYQMGFATFGVLSALAFGLVVLHRARWLEWALPKESAVVIEPIRAGISVDSGA
>Mature_465_residues
MKNLLNSLASGNWRALLACFLYFDTGFTVWVMFGPLAPFINKSIAMSPAELGFLVAVPVLGAAILRVTLGNLYQACDGRR
VALMGVALSAIPSIVLLLMPGAPSYTLLLILGVFLGVGGASFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFMFP
ALADHFGWAKAAGAALPLLGLAAVALFFWARDLGQKTGSGAQALRSFIVTLAGLVALVVAVHAGVFGAGKTGVLLLPVLG
ALLAIAVLPKHYRSVLVEGDTWVVMLVYSITFGGFVGMSSYVTTLLISLYQMPRLEAGLFMSLLACIGALVRPIGGLVAD
RISGVRALVILLAAISLCDFLFAAWMPPVSAGIALLIAMYVCFGLGNGATFQLVPQRWKGKTGLMSGIVGAAGGIGGFYL
PVIMGIAKEGTGSYQMGFATFGVLSALAFGLVVLHRARWLEWALPKESAVVIEPIRAGISVDSGA

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=192, Percent_Identity=28.125, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48074; Mature: 48074

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLNSLASGNWRALLACFLYFDTGFTVWVMFGPLAPFINKSIAMSPAELGFLVAVPVL
CHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCHHCCCHHHHHHHHHHHHH
GAAILRVTLGNLYQACDGRRVALMGVALSAIPSIVLLLMPGAPSYTLLLILGVFLGVGGA
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFMFPALADHFGWAKAAGAALPLLG
HHHEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAAVALFFWARDLGQKTGSGAQALRSFIVTLAGLVALVVAVHAGVFGAGKTGVLLLPVLG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ALLAIAVLPKHYRSVLVEGDTWVVMLVYSITFGGFVGMSSYVTTLLISLYQMPRLEAGLF
HHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MSLLACIGALVRPIGGLVADRISGVRALVILLAAISLCDFLFAAWMPPVSAGIALLIAMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VCFGLGNGATFQLVPQRWKGKTGLMSGIVGAAGGIGGFYLPVIMGIAKEGTGSYQMGFAT
HHHCCCCCCEEEECCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEHHHHH
FGVLSALAFGLVVLHRARWLEWALPKESAVVIEPIRAGISVDSGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCC
>Mature Secondary Structure
MKNLLNSLASGNWRALLACFLYFDTGFTVWVMFGPLAPFINKSIAMSPAELGFLVAVPVL
CHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCHHCCCHHHHHHHHHHHHH
GAAILRVTLGNLYQACDGRRVALMGVALSAIPSIVLLLMPGAPSYTLLLILGVFLGVGGA
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFMFPALADHFGWAKAAGAALPLLG
HHHEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAAVALFFWARDLGQKTGSGAQALRSFIVTLAGLVALVVAVHAGVFGAGKTGVLLLPVLG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ALLAIAVLPKHYRSVLVEGDTWVVMLVYSITFGGFVGMSSYVTTLLISLYQMPRLEAGLF
HHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MSLLACIGALVRPIGGLVADRISGVRALVILLAAISLCDFLFAAWMPPVSAGIALLIAMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VCFGLGNGATFQLVPQRWKGKTGLMSGIVGAAGGIGGFYLPVIMGIAKEGTGSYQMGFAT
HHHCCCCCCEEEECCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEHHHHH
FGVLSALAFGLVVLHRARWLEWALPKESAVVIEPIRAGISVDSGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]