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The map label for this gene is pyrC' [H]

Identifier: 186477074

GI number: 186477074

Start: 2607479

End: 2608756

Strand: Reverse

Name: pyrC' [H]

Synonym: Bphy_2323

Alternate gene names: 186477074

Gene position: 2608756-2607479 (Counterclockwise)

Preceding gene: 186477075

Following gene: 186477073

Centisome position: 74.98

GC content: 65.73

Gene sequence:

>1278_bases
ATGCAGATTCATATTCAAGGCGGCACGCTGATCGATCCGGTAGCGGGCACCGAGCAGCAGCAGGACGTATTCATCGCGGC
GGGGAAAATTGTCGCGCTCGGCAACGCGCCCGCCGATTTCAGCGCGGCGAGAACCATCGACGCGAAGGGCCTGTACGTGG
CACCGGGCTTCGTCGACCTGTCGGCGCGTCTGCGCGAACCCGGCTACGAGCACAAGGCGACGCTCGAATCGGAAATGGCC
GCGGCGCTCGCGGGCGGCGTGACGAGTCTCGTGTGTCCGCCCGACACCGACCCTGTGCTGGACGAGCCGGGCCTCATCGA
AATGCTCAAATTCCGCGCGCGCAATCTGAACCGCGCGCATGTGTATCCGCTCGGCGCGCTGACGGTTGGCCTCAAGGGAC
AGGTTATTACCGAAATGGTCGCGTTGACGGAATCGGGCTGCATCGGTTTTTCGCAGGCCGATACGCCCATCGTCGATACG
CAGGTGTTGCTGCGCGCGCTGCAGTACGCCAAGACCTACGGCTACACAGTCTGGCTGCGTCCGCAGGATGCGTATCTCGC
GAAGGGTGGCGTGGCGGCGAGCGGGCCGCTCGCGTCGCGGCTGGGTCTGTCGGGCGTGCCCGTGTCGGCGGAAACGATCG
CGCTGCATACGATCTTCGAACTCGTGCGCGTGACGGGTGCGCGCGTGCATCTGTCGCACGTGTCGTCGGCGGCGGGCGTT
GAACTTATGCGCGCGGCGAAAGCGGAAGGCTTGCCCGTGAGTTGCGACGTCACGATCAATCATGTGCATCTGATCGATGT
CGACATTGGCTACTTCGATGCGCAGTTCCGGCTCGATCCGCCGCTGCGTTCGCAGCGCGACCGCGAAGCGATCCGCGCGG
GCCTGCTGGACGGAACGATCGACGCAATCTGCTCGGACCACACGCCCGTCGACGACGATGAAAAGCTGCTGCCCTTCGCC
GAAGCGACGCCCGGCGCGACGGGGCTCGAATTGCTGCTCTCGCTTACCGTCAAGTGGGCCGACGAGGCGGGCATCCCGCT
CGCGAAGGCGCTGAACCGCATCACGGCTGCGCCCGCTGGCGTGTTGAAGCTGCCTGCTGGGCGCATCGAAGCCGGCGCAA
CCGCCGACCTGTGCGTGTTCGACCGCGACGTCCATTGGCGCATCGAGCCGCGCGCGTTGAAAAGCCAGGGGCACAACACG
CCGTTTCTCGGCTATGAATTGCCAGCGCGCGTGCGCGCGACGGTCGTATCGGGCCAGGTTGCGTTCGAGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGTTCGGCATCGCGGTTCGCATGGCGGTGATGGGCATCGTCGCGGGCAACAACGACTGATCCCCATCCGTTCCAGAGACA
GCAGGCAAATAAGGCAGCGC

Downstream 100 bases:

>100_bases
GCCGACTGATACCCGGCCGAGATTGAAAAGATAAGCGGAAGACTCGAAACATGAAGCTCGCGTTTCGCAAGATTCGTTTG
GTGATGCATCTCGTGTACGG

Product: dihydroorotase

Products: Allantoate; H+ [C]

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MQIHIQGGTLIDPVAGTEQQQDVFIAAGKIVALGNAPADFSAARTIDAKGLYVAPGFVDLSARLREPGYEHKATLESEMA
AALAGGVTSLVCPPDTDPVLDEPGLIEMLKFRARNLNRAHVYPLGALTVGLKGQVITEMVALTESGCIGFSQADTPIVDT
QVLLRALQYAKTYGYTVWLRPQDAYLAKGGVAASGPLASRLGLSGVPVSAETIALHTIFELVRVTGARVHLSHVSSAAGV
ELMRAAKAEGLPVSCDVTINHVHLIDVDIGYFDAQFRLDPPLRSQRDREAIRAGLLDGTIDAICSDHTPVDDDEKLLPFA
EATPGATGLELLLSLTVKWADEAGIPLAKALNRITAAPAGVLKLPAGRIEAGATADLCVFDRDVHWRIEPRALKSQGHNT
PFLGYELPARVRATVVSGQVAFEQR

Sequences:

>Translated_425_residues
MQIHIQGGTLIDPVAGTEQQQDVFIAAGKIVALGNAPADFSAARTIDAKGLYVAPGFVDLSARLREPGYEHKATLESEMA
AALAGGVTSLVCPPDTDPVLDEPGLIEMLKFRARNLNRAHVYPLGALTVGLKGQVITEMVALTESGCIGFSQADTPIVDT
QVLLRALQYAKTYGYTVWLRPQDAYLAKGGVAASGPLASRLGLSGVPVSAETIALHTIFELVRVTGARVHLSHVSSAAGV
ELMRAAKAEGLPVSCDVTINHVHLIDVDIGYFDAQFRLDPPLRSQRDREAIRAGLLDGTIDAICSDHTPVDDDEKLLPFA
EATPGATGLELLLSLTVKWADEAGIPLAKALNRITAAPAGVLKLPAGRIEAGATADLCVFDRDVHWRIEPRALKSQGHNT
PFLGYELPARVRATVVSGQVAFEQR
>Mature_425_residues
MQIHIQGGTLIDPVAGTEQQQDVFIAAGKIVALGNAPADFSAARTIDAKGLYVAPGFVDLSARLREPGYEHKATLESEMA
AALAGGVTSLVCPPDTDPVLDEPGLIEMLKFRARNLNRAHVYPLGALTVGLKGQVITEMVALTESGCIGFSQADTPIVDT
QVLLRALQYAKTYGYTVWLRPQDAYLAKGGVAASGPLASRLGLSGVPVSAETIALHTIFELVRVTGARVHLSHVSSAAGV
ELMRAAKAEGLPVSCDVTINHVHLIDVDIGYFDAQFRLDPPLRSQRDREAIRAGLLDGTIDAICSDHTPVDDDEKLLPFA
EATPGATGLELLLSLTVKWADEAGIPLAKALNRITAAPAGVLKLPAGRIEAGATADLCVFDRDVHWRIEPRALKSQGHNT
PFLGYELPARVRATVVSGQVAFEQR

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=215, Percent_Identity=30.6976744186047, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI19923821, Length=466, Percent_Identity=26.1802575107296, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1786722, Length=456, Percent_Identity=27.1929824561404, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082175, Length=441, Percent_Identity=24.2630385487528, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=398, Percent_Identity=25.3768844221106, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322218, Length=439, Percent_Identity=23.9179954441913, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=26.1421319796954, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI18859883, Length=212, Percent_Identity=28.3018867924528, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.5 [C]

Molecular weight: Translated: 45165; Mature: 45165

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIHIQGGTLIDPVAGTEQQQDVFIAAGKIVALGNAPADFSAARTIDAKGLYVAPGFVDL
CEEEECCCCEECCCCCCCCCCCEEEEECCEEEECCCCCCCCHHEEECCCCEEECCCCEEH
SARLREPGYEHKATLESEMAAALAGGVTSLVCPPDTDPVLDEPGLIEMLKFRARNLNRAH
HHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYPLGALTVGLKGQVITEMVALTESGCIGFSQADTPIVDTQVLLRALQYAKTYGYTVWLR
EEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCEEEEEC
PQDAYLAKGGVAASGPLASRLGLSGVPVSAETIALHTIFELVRVTGARVHLSHVSSAAGV
CCCCCEECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHH
ELMRAAKAEGLPVSCDVTINHVHLIDVDIGYFDAQFRLDPPLRSQRDREAIRAGLLDGTI
HHHHHHHCCCCCEEEEEEEEEEEEEEEECCEEEEEEEECCCCCCHHHHHHHHHHCCCCCH
DAICSDHTPVDDDEKLLPFAEATPGATGLELLLSLTVKWADEAGIPLAKALNRITAAPAG
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCC
VLKLPAGRIEAGATADLCVFDRDVHWRIEPRALKSQGHNTPFLGYELPARVRATVVSGQV
EEECCCCCCCCCCCEEEEEEECCEEEEECCHHHHCCCCCCCEECEECCCCEEEEEEECEE
AFEQR
EECCC
>Mature Secondary Structure
MQIHIQGGTLIDPVAGTEQQQDVFIAAGKIVALGNAPADFSAARTIDAKGLYVAPGFVDL
CEEEECCCCEECCCCCCCCCCCEEEEECCEEEECCCCCCCCHHEEECCCCEEECCCCEEH
SARLREPGYEHKATLESEMAAALAGGVTSLVCPPDTDPVLDEPGLIEMLKFRARNLNRAH
HHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYPLGALTVGLKGQVITEMVALTESGCIGFSQADTPIVDTQVLLRALQYAKTYGYTVWLR
EEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCEEEEEC
PQDAYLAKGGVAASGPLASRLGLSGVPVSAETIALHTIFELVRVTGARVHLSHVSSAAGV
CCCCCEECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHH
ELMRAAKAEGLPVSCDVTINHVHLIDVDIGYFDAQFRLDPPLRSQRDREAIRAGLLDGTI
HHHHHHHCCCCCEEEEEEEEEEEEEEEECCEEEEEEEECCCCCCHHHHHHHHHHCCCCCH
DAICSDHTPVDDDEKLLPFAEATPGATGLELLLSLTVKWADEAGIPLAKALNRITAAPAG
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCC
VLKLPAGRIEAGATADLCVFDRDVHWRIEPRALKSQGHNTPFLGYELPARVRATVVSGQV
EEECCCCCCCCCCCEEEEEEECCEEEEECCHHHHCCCCCCCEECEECCCCEEEEEEECEE
AFEQR
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Allantoin; H2O [C]

Specific reaction: Allantoin + H2O --> Allantoate + H+ [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; p-Chloromercuribenzene-sulphonic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]