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The map label for this gene is pyrB [H]

Identifier: 186477075

GI number: 186477075

Start: 2608798

End: 2609838

Strand: Reverse

Name: pyrB [H]

Synonym: Bphy_2324

Alternate gene names: 186477075

Gene position: 2609838-2608798 (Counterclockwise)

Preceding gene: 186477076

Following gene: 186477074

Centisome position: 75.01

GC content: 62.54

Gene sequence:

>1041_bases
ATGAACACGCCCAACCCGGTTTCCCAAGGTTCCACCGTTTCCGCGGATAACCCCGCGCCAGCGGACCGTTTTCGCTACGG
TTTCCTGAAGGGCAATCCGCAGCTCACGAAGAACGGCGAGCTGAAGCATCTGCTCACCATCGAGGGACTGCCGCGCGCAA
TCGTCACGCACATTCTCGATACGGCCGAGCAGTTCGTCAGCGTGACCGACCGCGAGGTGAAGAAGGTGCCGCTGCTGCGC
GGCAAATCGGTATTCAACCTGTTTTTCGAGAACTCGACGCGCACGCGCACCACGTTCGAAATCGCCGCGAAACGGCTGTC
GGCGGACGTGATCAACCTGAACATCAACGCGTCGTCGACGAGCAAGGGCGAATCGCTGCTCGACACGATCAACAACCTGT
CGGCGATGCATGCCGACATGTTCGTCGTGCGTCACGCGTCGAGTGGCGCGCCGTATCTGATCGCCGAGCATTGTGCGCCG
CACGTGCACGTGATCAACGCGGGTGACGGCCGCCACGCACATCCGACGCAGGGCCTGCTCGACATGTACACGATCCGCCA
CTACAAGCGCGATTTCACCAATCTGCGCGTGGCGATCGTCGGCGACATTCTGCATTCGCGCGTTGCACGTTCGGACATCC
ACGCGCTCACCACGCTCGGCGTACCCGAAGTGCGCGCGATCGGTCCGCGTACGCTGCTGCCGGGCGGCCTCGAGCAGATG
GGGGTGCGCGTGTTCCATAACCTCGACGAAGGGTTGAAGGACGTTGACGTGATCATCATGCTGCGTCTGCAGAACGAGCG
GATGAGCGGTGCATTGCTGCCGTCGGCGCAGGAATACTTCAAGAGCTGGGGGCTGACGCCCGAGCGTCTCGCGCTGGCGA
AGCCCGACGCGATCGTGATGCATCCGGGACCGATGAACCGCGGCGTCGAGATCGATTCGCAGGTGGCGGACGGTCCGCAA
TCGGTGATTCTCAATCAGGTGACGTTCGGCATCGCGGTTCGCATGGCGGTGATGGGCATCGTCGCGGGCAACAACGACTG
A

Upstream 100 bases:

>100_bases
TTCACTTTTCACACCGAGCCGCGTGCGCATTGAATGCGGCGCTTCGGCATCGAAAGCAATACGGGCAGCACACGCAGTCC
CAGTCTGATTCAGGAACACC

Downstream 100 bases:

>100_bases
TCCCCATCCGTTCCAGAGACAGCAGGCAAATAAGGCAGCGCATGCAGATTCATATTCAAGGCGGCACGCTGATCGATCCG
GTAGCGGGCACCGAGCAGCA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNTPNPVSQGSTVSADNPAPADRFRYGFLKGNPQLTKNGELKHLLTIEGLPRAIVTHILDTAEQFVSVTDREVKKVPLLR
GKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMHADMFVVRHASSGAPYLIAEHCAP
HVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTNLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQM
GVRVFHNLDEGLKDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAKPDAIVMHPGPMNRGVEIDSQVADGPQ
SVILNQVTFGIAVRMAVMGIVAGNND

Sequences:

>Translated_346_residues
MNTPNPVSQGSTVSADNPAPADRFRYGFLKGNPQLTKNGELKHLLTIEGLPRAIVTHILDTAEQFVSVTDREVKKVPLLR
GKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMHADMFVVRHASSGAPYLIAEHCAP
HVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTNLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQM
GVRVFHNLDEGLKDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAKPDAIVMHPGPMNRGVEIDSQVADGPQ
SVILNQVTFGIAVRMAVMGIVAGNND
>Mature_346_residues
MNTPNPVSQGSTVSADNPAPADRFRYGFLKGNPQLTKNGELKHLLTIEGLPRAIVTHILDTAEQFVSVTDREVKKVPLLR
GKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMHADMFVVRHASSGAPYLIAEHCAP
HVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTNLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQM
GVRVFHNLDEGLKDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAKPDAIVMHPGPMNRGVEIDSQVADGPQ
SVILNQVTFGIAVRMAVMGIVAGNND

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=34.6153846153846, Blast_Score=165, Evalue=5e-41,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=31.2312312312312, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=30.9904153354633, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24642586, Length=320, Percent_Identity=34.0625, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 37794; Mature: 37794

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPNPVSQGSTVSADNPAPADRFRYGFLKGNPQLTKNGELKHLLTIEGLPRAIVTHILD
CCCCCCCCCCCEECCCCCCCHHHHEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
TAEQFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASST
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
SKGESLLDTINNLSAMHADMFVVRHASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLL
CCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHH
DMYTIRHYKRDFTNLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQM
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHH
GVRVFHNLDEGLKDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAKPDAIVM
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHEECCCCEEEE
HPGPMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGNND
CCCCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MNTPNPVSQGSTVSADNPAPADRFRYGFLKGNPQLTKNGELKHLLTIEGLPRAIVTHILD
CCCCCCCCCCCEECCCCCCCHHHHEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
TAEQFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASST
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
SKGESLLDTINNLSAMHADMFVVRHASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLL
CCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHH
DMYTIRHYKRDFTNLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQM
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHH
GVRVFHNLDEGLKDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAKPDAIVM
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHEECCCCEEEE
HPGPMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGNND
CCCCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA