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The map label for this gene is tagO [H]

Identifier: 186477041

GI number: 186477041

Start: 2569410

End: 2570438

Strand: Reverse

Name: tagO [H]

Synonym: Bphy_2290

Alternate gene names: 186477041

Gene position: 2570438-2569410 (Counterclockwise)

Preceding gene: 186477042

Following gene: 186477040

Centisome position: 73.88

GC content: 66.47

Gene sequence:

>1029_bases
ATGCAGATGCAAGATTCACTGATGTCTCCGTGGACTCTATCCCTCGTCGCGGGCGTGGCCGTCTGTGCCTGCGCGGCGAT
CCTGTACCTGCTGCTGAAGACAGGTCTCGCCTGGCGCCTCGCGACCGATATCCCGAACGACCGCTCGCTCCACGTGCGGC
CGACGCCGCGCGTCGGCGGCTGGGGCATCGTGCCCGTCGCGGTCGTCGCGATGCTGCTGATCGCGCCGTCGCTGTGGTTC
GTCGGGCTGGCGACGGCGTTTCTCGCCGCCGTATCGCAGGTTGACGACCGGCGCGGCTTGCCCGCGCGCGTGCGTTTCGC
CGCGCATCTGATCGCCGTCGTCGCGTTCGTCGCACTGTTTCCGGCTGCGGCGCCGTGGTGGGTGCTGGTCGTGCTGGCAT
TTTTGATGATCTGGCTGGTCAATCTCTACAATTTCATGGACGGCGCCGACGGCCTCGCGGGCGGCATGGCGCTGTTCGGC
TTCGCGGGCTACGCGGCGGCGGCGCTGCTCTCCGATCATCCGATGCCGGAACTGGCACTTGCGTCGCTGGCGATTGCGGG
CGCCGCGCTCGGTTTCCTGTTCTTCAATTTTCATCCGGCGCGGATTTTTCTCGGCGACGCAGGCTCGATCTCGCTCGGTT
ATCTGGCGGGCGCGCTCGGCTACTGGGGCTGGCGCGGCGGCGCATGGCCCGTGTGGTTCCCGGCGCTCGTGTTCTCGCCG
TTTATCGCCGACGCGTCGGTGACCCTCGCGCGGCGCCTTGCGCGCGGTGAAAAGTTTTGGCAAGCGCACCGCGAGCACTA
CTATCAACGCATGGTGCGTTCCGGCGTCGGTCATGCCGGAACCGCATGGGTGTGGTATGCAGTGATGGTCGCCGGCATCG
CCCTTGCTTTATTCGCGCTCGGCCGTTCTACGGCCACTCAGTGGGGCATCGTCGCGGGATGGGCCGGCGTGCTGGTCGTC
GTCGGAAGCATCGTCGATCTGCGCTGGCGCCGTTTCCAGTCCACACTGTGCAAACAATCCGAGGTATGA

Upstream 100 bases:

>100_bases
TCGCACATACGCGACGTCCTCGGCTGGCAACCGCCGTTCTCAACCGACGAAGGTCTGGCAGCAACCGCCGACTGGTATCG
TTCGACTCACTGAACCGTTC

Downstream 100 bases:

>100_bases
CGCTGATGCTCAGATCCAAAGCCAGCTGGCTATCCTTCAGCGCTTTTCTGTTCGACCTGGCTGCCGTCGCCGGCACGTGG
CTCATCGCCTACGTGATCCG

Product: glycosyl transferase family protein

Products: Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine; UMP [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-Phosphate GlcNAc-1-phosphate transferase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MQMQDSLMSPWTLSLVAGVAVCACAAILYLLLKTGLAWRLATDIPNDRSLHVRPTPRVGGWGIVPVAVVAMLLIAPSLWF
VGLATAFLAAVSQVDDRRGLPARVRFAAHLIAVVAFVALFPAAAPWWVLVVLAFLMIWLVNLYNFMDGADGLAGGMALFG
FAGYAAAALLSDHPMPELALASLAIAGAALGFLFFNFHPARIFLGDAGSISLGYLAGALGYWGWRGGAWPVWFPALVFSP
FIADASVTLARRLARGEKFWQAHREHYYQRMVRSGVGHAGTAWVWYAVMVAGIALALFALGRSTATQWGIVAGWAGVLVV
VGSIVDLRWRRFQSTLCKQSEV

Sequences:

>Translated_342_residues
MQMQDSLMSPWTLSLVAGVAVCACAAILYLLLKTGLAWRLATDIPNDRSLHVRPTPRVGGWGIVPVAVVAMLLIAPSLWF
VGLATAFLAAVSQVDDRRGLPARVRFAAHLIAVVAFVALFPAAAPWWVLVVLAFLMIWLVNLYNFMDGADGLAGGMALFG
FAGYAAAALLSDHPMPELALASLAIAGAALGFLFFNFHPARIFLGDAGSISLGYLAGALGYWGWRGGAWPVWFPALVFSP
FIADASVTLARRLARGEKFWQAHREHYYQRMVRSGVGHAGTAWVWYAVMVAGIALALFALGRSTATQWGIVAGWAGVLVV
VGSIVDLRWRRFQSTLCKQSEV
>Mature_342_residues
MQMQDSLMSPWTLSLVAGVAVCACAAILYLLLKTGLAWRLATDIPNDRSLHVRPTPRVGGWGIVPVAVVAMLLIAPSLWF
VGLATAFLAAVSQVDDRRGLPARVRFAAHLIAVVAFVALFPAAAPWWVLVVLAFLMIWLVNLYNFMDGADGLAGGMALFG
FAGYAAAALLSDHPMPELALASLAIAGAALGFLFFNFHPARIFLGDAGSISLGYLAGALGYWGWRGGAWPVWFPALVFSP
FIADASVTLARRLARGEKFWQAHREHYYQRMVRSGVGHAGTAWVWYAVMVAGIALALFALGRSTATQWGIVAGWAGVLVV
VGSIVDLRWRRFQSTLCKQSEV

Specific function: Catalyzes the formation of undecaprenyl-PP-N- acetylglucosamine. Involved in the synthesis of anionic cell-wall polymers as it mediates the initiation of the linkage unit formation that appears to be common to the two types of teichoic acids attached to t

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1786275, Length=216, Percent_Identity=32.8703703703704, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000715
- InterPro:   IPR018480 [H]

Pfam domain/function: PF00953 Glycos_transf_4 [H]

EC number: 2.7.8.13 [C]

Molecular weight: Translated: 36844; Mature: 36844

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMQDSLMSPWTLSLVAGVAVCACAAILYLLLKTGLAWRLATDIPNDRSLHVRPTPRVGG
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCEEEECCCCCCCC
WGIVPVAVVAMLLIAPSLWFVGLATAFLAAVSQVDDRRGLPARVRFAAHLIAVVAFVALF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PAAAPWWVLVVLAFLMIWLVNLYNFMDGADGLAGGMALFGFAGYAAAALLSDHPMPELAL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ASLAIAGAALGFLFFNFHPARIFLGDAGSISLGYLAGALGYWGWRGGAWPVWFPALVFSP
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FIADASVTLARRLARGEKFWQAHREHYYQRMVRSGVGHAGTAWVWYAVMVAGIALALFAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
GRSTATQWGIVAGWAGVLVVVGSIVDLRWRRFQSTLCKQSEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQMQDSLMSPWTLSLVAGVAVCACAAILYLLLKTGLAWRLATDIPNDRSLHVRPTPRVGG
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCEEEECCCCCCCC
WGIVPVAVVAMLLIAPSLWFVGLATAFLAAVSQVDDRRGLPARVRFAAHLIAVVAFVALF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PAAAPWWVLVVLAFLMIWLVNLYNFMDGADGLAGGMALFGFAGYAAAALLSDHPMPELAL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ASLAIAGAALGFLFFNFHPARIFLGDAGSISLGYLAGALGYWGWRGGAWPVWFPALVFSP
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FIADASVTLARRLARGEKFWQAHREHYYQRMVRSGVGHAGTAWVWYAVMVAGIALALFAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
GRSTATQWGIVAGWAGVLVVVGSIVDLRWRRFQSTLCKQSEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Undecaprenyl phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine [C]

Specific reaction: Undecaprenyl phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine --> Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP [C]

General reaction: Substituted phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12101296; 9384377 [H]