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The map label for this gene is cysN [H]

Identifier: 186476982

GI number: 186476982

Start: 2503497

End: 2504825

Strand: Reverse

Name: cysN [H]

Synonym: Bphy_2231

Alternate gene names: 186476982

Gene position: 2504825-2503497 (Counterclockwise)

Preceding gene: 186476983

Following gene: 186476981

Centisome position: 71.99

GC content: 64.48

Gene sequence:

>1329_bases
ATGAGCCGCATTGAGCAACCCGCGCATCAACCTGAAGACCTCGGCGTGCTTCGTTTCATCACGGCAGGCAGCGTCGACGA
CGGCAAGAGCACGCTGATCGGCAGGCTGCTGTACGACAGCAAGGCCGTGCTGTCGGATCAGCTGTCGGCGCTGTCGCGCG
CGAAGAACAAGCGCACGGTCGGCGACGAAATCGACCTGTCGCTGCTCACGGACGGCCTCGAAGCCGAGCGCGAGCAGGGC
ATCACGATCGATGTCGCGTATCGCTACTTCGCGACGGCCAGGCGCAAGTTCATCATCGCCGACACGCCGGGCCACGAGCA
GTACACGCGCAATATGGTAACGGGCGCGTCGACGGCGCACGCGGCCATCATTCTTGTCGACGCGACGCGCGTCACGTTCG
AGAACGACGAAGCGCAACTGCTGCCGCAAACCAAGCGCCACAGCGCGATCGTGAAGCTGCTCGGCTTGCAGCACGTGATC
GTCGCGATCAACAAGATGGATCTCGTCGAGTACAGCGAAGCACGCTTCAACGAGATTCGCGATGCGTATGTCGCGCTGGC
ACGCCAACTCGGTTTGAGCGATGTGCGCTTCGTGCCCGTGTCGGCCCTGAAGGGCGACAACATCGTGACGGCGAGCGAAC
GCATGCCGTGGTATGCGGGCGAGCCGCTGCTCGACGTGCTGGAGGCACTGCCCGTCGATACAGCGACGCATGAAGCGCTG
CGTTTCCCGGTGCAATGGGTCGCGCGCCAGGACGGCAGCCAGGCCGACGATTTCCGCGGCTACATGGGCCGCATCGAATC
GGGCGAAGTGAAGCTCGGCGATTCGATCGTCGTGCTGCCGGCAAACCGCACGGCGACCGTCGCTGAGATCATCGCGCCCG
TGCCGGGCGGCACGGCGCAAGTGGACCGCGCGTTCGCGGGCCAGACGGTCACGCTCCGTCTCGCGGAAGACGTCGATGTG
TCGCGCGGCGATACGTTCGTGCTCGCTTCCGACCCTGTGCAGCCGGCCAAAAAGCTCGAAGCGGACCTGTGCTGGTTCGA
CGAGACGCCGCTGTCGACGGCTCGCAAGTATCTGCTGAAGCAGACCACGAACACGGTGTTTGCGCGTGTCGGCGGTATCC
AGCAGGTACTGGACGTGCACACGTTGTCGCACGCGACGGACCGCCACGAACTGGCGATGAACGATATCGGCCGCGTGTCG
CTGACGCTGCAAAAGCCGATCGTCGCGGACGAGTACGACGCGCATCCGGGCACGGGCGCCTTTGTGCTGATCGACGAAGC
GACCCATCACACGGTTGCGGCGGGCATGATCCGTTCGGTTTTCGCCTGA

Upstream 100 bases:

>100_bases
CGTGGCGCGACGCGCATGGACGACCAGACGTCGGAAGCAGCGATGGAACAGCGCAAGAAGCAAGGTTATTTCTAAAGAGC
ACGCAGAGGAAAGAGTCAAC

Downstream 100 bases:

>100_bases
AGCATTTGAAGTTCGCAGTGGAACGGAGCCAGCAAATGGGTAAGGTGTATCTGATCGGCGCGGGGCCGGGTGCGGCGGAC
CTGATCACGGTTCGCGGTGC

Product: sulfate adenylyltransferase large subunit

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSRIEQPAHQPEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEIDLSLLTDGLEAEREQG
ITIDVAYRYFATARRKFIIADTPGHEQYTRNMVTGASTAHAAIILVDATRVTFENDEAQLLPQTKRHSAIVKLLGLQHVI
VAINKMDLVEYSEARFNEIRDAYVALARQLGLSDVRFVPVSALKGDNIVTASERMPWYAGEPLLDVLEALPVDTATHEAL
RFPVQWVARQDGSQADDFRGYMGRIESGEVKLGDSIVVLPANRTATVAEIIAPVPGGTAQVDRAFAGQTVTLRLAEDVDV
SRGDTFVLASDPVQPAKKLEADLCWFDETPLSTARKYLLKQTTNTVFARVGGIQQVLDVHTLSHATDRHELAMNDIGRVS
LTLQKPIVADEYDAHPGTGAFVLIDEATHHTVAAGMIRSVFA

Sequences:

>Translated_442_residues
MSRIEQPAHQPEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEIDLSLLTDGLEAEREQG
ITIDVAYRYFATARRKFIIADTPGHEQYTRNMVTGASTAHAAIILVDATRVTFENDEAQLLPQTKRHSAIVKLLGLQHVI
VAINKMDLVEYSEARFNEIRDAYVALARQLGLSDVRFVPVSALKGDNIVTASERMPWYAGEPLLDVLEALPVDTATHEAL
RFPVQWVARQDGSQADDFRGYMGRIESGEVKLGDSIVVLPANRTATVAEIIAPVPGGTAQVDRAFAGQTVTLRLAEDVDV
SRGDTFVLASDPVQPAKKLEADLCWFDETPLSTARKYLLKQTTNTVFARVGGIQQVLDVHTLSHATDRHELAMNDIGRVS
LTLQKPIVADEYDAHPGTGAFVLIDEATHHTVAAGMIRSVFA
>Mature_441_residues
SRIEQPAHQPEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEIDLSLLTDGLEAEREQGI
TIDVAYRYFATARRKFIIADTPGHEQYTRNMVTGASTAHAAIILVDATRVTFENDEAQLLPQTKRHSAIVKLLGLQHVIV
AINKMDLVEYSEARFNEIRDAYVALARQLGLSDVRFVPVSALKGDNIVTASERMPWYAGEPLLDVLEALPVDTATHEALR
FPVQWVARQDGSQADDFRGYMGRIESGEVKLGDSIVVLPANRTATVAEIIAPVPGGTAQVDRAFAGQTVTLRLAEDVDVS
RGDTFVLASDPVQPAKKLEADLCWFDETPLSTARKYLLKQTTNTVFARVGGIQQVLDVHTLSHATDRHELAMNDIGRVSL
TLQKPIVADEYDAHPGTGAFVLIDEATHHTVAAGMIRSVFA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=29.9771167048055, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=29.9771167048055, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=27.7161862527716, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=27.7161862527716, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI194018522, Length=439, Percent_Identity=27.7904328018223, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=28.246013667426, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194097354, Length=321, Percent_Identity=30.8411214953271, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194018520, Length=321, Percent_Identity=30.8411214953271, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI34147630, Length=335, Percent_Identity=28.3582089552239, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789108, Length=428, Percent_Identity=49.7663551401869, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI2367247, Length=360, Percent_Identity=25.2777777777778, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790412, Length=341, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789737, Length=341, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17552884, Length=447, Percent_Identity=28.8590604026846, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=447, Percent_Identity=28.8590604026846, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI115532067, Length=349, Percent_Identity=33.5243553008596, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI115532065, Length=349, Percent_Identity=33.5243553008596, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32566629, Length=443, Percent_Identity=28.4424379232506, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=447, Percent_Identity=26.8456375838926, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32566301, Length=144, Percent_Identity=31.9444444444444, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=270, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=343, Percent_Identity=27.4052478134111, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322937, Length=327, Percent_Identity=31.8042813455657, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=28.0898876404494, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=28.0898876404494, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=447, Percent_Identity=29.3064876957494, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324761, Length=277, Percent_Identity=28.5198555956679, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24652838, Length=460, Percent_Identity=30, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI17137572, Length=460, Percent_Identity=30, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.4642857142857, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.4642857142857, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.4642857142857, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.4642857142857, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45550900, Length=451, Percent_Identity=29.0465631929047, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17137380, Length=434, Percent_Identity=25.3456221198157, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI281363316, Length=267, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17864358, Length=267, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19921738, Length=175, Percent_Identity=30.8571428571429, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 48305; Mature: 48174

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIEQPAHQPEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTV
CCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GDEIDLSLLTDGLEAEREQGITIDVAYRYFATARRKFIIADTPGHEQYTRNMVTGASTAH
CCCCCHHHHHCCHHHHHHCCCEEEEHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCC
AAIILVDATRVTFENDEAQLLPQTKRHSAIVKLLGLQHVIVAINKMDLVEYSEARFNEIR
EEEEEEEEEEEEEECCCHHCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
DAYVALARQLGLSDVRFVPVSALKGDNIVTASERMPWYAGEPLLDVLEALPVDTATHEAL
HHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHH
RFPVQWVARQDGSQADDFRGYMGRIESGEVKLGDSIVVLPANRTATVAEIIAPVPGGTAQ
HCCHHHHHHCCCCCCHHHHHHHCCCCCCCEEECCEEEEEECCCCHHHHHHHHCCCCCHHH
VDRAFAGQTVTLRLAEDVDVSRGDTFVLASDPVQPAKKLEADLCWFDETPLSTARKYLLK
HHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHCCCCEEECCCCCHHHHHHHHHH
QTTNTVFARVGGIQQVLDVHTLSHATDRHELAMNDIGRVSLTLQKPIVADEYDAHPGTGA
HHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCE
FVLIDEATHHTVAAGMIRSVFA
EEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIEQPAHQPEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTV
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GDEIDLSLLTDGLEAEREQGITIDVAYRYFATARRKFIIADTPGHEQYTRNMVTGASTAH
CCCCCHHHHHCCHHHHHHCCCEEEEHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCC
AAIILVDATRVTFENDEAQLLPQTKRHSAIVKLLGLQHVIVAINKMDLVEYSEARFNEIR
EEEEEEEEEEEEEECCCHHCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
DAYVALARQLGLSDVRFVPVSALKGDNIVTASERMPWYAGEPLLDVLEALPVDTATHEAL
HHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHH
RFPVQWVARQDGSQADDFRGYMGRIESGEVKLGDSIVVLPANRTATVAEIIAPVPGGTAQ
HCCHHHHHHCCCCCCHHHHHHHCCCCCCCEEECCEEEEEECCCCHHHHHHHHCCCCCHHH
VDRAFAGQTVTLRLAEDVDVSRGDTFVLASDPVQPAKKLEADLCWFDETPLSTARKYLLK
HHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHCCCCEEECCCCCHHHHHHHHHH
QTTNTVFARVGGIQQVLDVHTLSHATDRHELAMNDIGRVSLTLQKPIVADEYDAHPGTGA
HHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCE
FVLIDEATHHTVAAGMIRSVFA
EEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA