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The map label for this gene is bla [H]

Identifier: 186476980

GI number: 186476980

Start: 2501663

End: 2502508

Strand: Reverse

Name: bla [H]

Synonym: Bphy_2229

Alternate gene names: 186476980

Gene position: 2502508-2501663 (Counterclockwise)

Preceding gene: 186476981

Following gene: 186476976

Centisome position: 71.93

GC content: 64.78

Gene sequence:

>846_bases
ATGATCGGCGTACGTGCGCAGCCGGCTTCCTCCGTATCGATCAATCTTGCTGCGCGTGAGCGCTTCGAACAACTGGAGGC
ATCGAGTGGCGGCCGGCTCGGCGTCGCGGCGCTCAGAACGGCTGACGGTTCCTATGTCGGCTACCGGGAGTCGGAACGCT
TTCCGATGTGCAGTACTTTCAAGCTGCTCGCCGTCGCATTGATCCTGAAGCGCAGCATGGCCGAACGCGGCTTGCTCGAA
GAGCGCATCCGCTACGGCGACGCTCAGGTCGTCGCTCATTCGCCCGTCACGAAGCGGCACGCAGGCGGCGAGGGAATGAC
GGTAGGGGAGTTGAGTGCAGCGGCGCTCCAGCATAGCGACAACACCGCGGCCAATCTGTTGCTGACGTCGCTTGGCGGGC
CGGAGGTGCTGAACCAGTTTGCGTTATCGATCGGCGACACCTGGTTCGACCTCGTTCGCAACGAACCCGAAGTGAACGCA
TCGGTGCCCGGGGACATGCGTGACACCACGTCGCCGCGCGCGATGCTGCTCGATGTGCAGAAACTGCTGCTCGCAGACGA
CGTGCTGGGTGCGCGCGAGCGCGAGCAGTTGAAGGCGTGGATGCTGGGCAATACGACGGGTGCCACGCGCATCCGCGCGG
CCGTGCCTGCGGCCGGATGGTTGGTCGCGGACAACACGGGCAGTGGAGACTACGGCACGGCTAACGACGTCGCCGTGGTA
TATCCGCCCTCGTCGGCGCCGTTGGTCGTCGCGATCTACTTCACGGGCGTGACGCCGAAGGAAACGCCGCCACAGGACGC
AATCGTCGACGAGGCGGCGCGAATCGTATTCGACGCGCTGCGATGA

Upstream 100 bases:

>100_bases
GCATTGCGATAATGCTTTCCCTTCGCTTTGCATTCAGTCTTCGCTCATGTCTTTTTCTTCTCTGCGTCGTTCACTGCTGA
TTGCGCTTGCGGCTGGTCCC

Downstream 100 bases:

>100_bases
ACGATGCAGATGCAGGAGCTAACGGTGTGAGCGGCATTCTTTTGCGAAGGCATAGCGTGGATGCCAGCGACGAAAACGCA
AACCGACGGCAGCGAAATCA

Product: beta-lactamase

Products: NA

Alternate protein names: Beta-lactamase MEN-1; Cefotaximase 1 [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MIGVRAQPASSVSINLAARERFEQLEASSGGRLGVAALRTADGSYVGYRESERFPMCSTFKLLAVALILKRSMAERGLLE
ERIRYGDAQVVAHSPVTKRHAGGEGMTVGELSAAALQHSDNTAANLLLTSLGGPEVLNQFALSIGDTWFDLVRNEPEVNA
SVPGDMRDTTSPRAMLLDVQKLLLADDVLGAREREQLKAWMLGNTTGATRIRAAVPAAGWLVADNTGSGDYGTANDVAVV
YPPSSAPLVVAIYFTGVTPKETPPQDAIVDEAARIVFDALR

Sequences:

>Translated_281_residues
MIGVRAQPASSVSINLAARERFEQLEASSGGRLGVAALRTADGSYVGYRESERFPMCSTFKLLAVALILKRSMAERGLLE
ERIRYGDAQVVAHSPVTKRHAGGEGMTVGELSAAALQHSDNTAANLLLTSLGGPEVLNQFALSIGDTWFDLVRNEPEVNA
SVPGDMRDTTSPRAMLLDVQKLLLADDVLGAREREQLKAWMLGNTTGATRIRAAVPAAGWLVADNTGSGDYGTANDVAVV
YPPSSAPLVVAIYFTGVTPKETPPQDAIVDEAARIVFDALR
>Mature_281_residues
MIGVRAQPASSVSINLAARERFEQLEASSGGRLGVAALRTADGSYVGYRESERFPMCSTFKLLAVALILKRSMAERGLLE
ERIRYGDAQVVAHSPVTKRHAGGEGMTVGELSAAALQHSDNTAANLLLTSLGGPEVLNQFALSIGDTWFDLVRNEPEVNA
SVPGDMRDTTSPRAMLLDVQKLLLADDVLGAREREQLKAWMLGNTTGATRIRAAVPAAGWLVADNTGSGDYGTANDVAVV
YPPSSAPLVVAIYFTGVTPKETPPQDAIVDEAARIVFDALR

Specific function: Broad spectrum beta-lactamase which confers resistance to penicillins, as well as first, second and third-generation cephalosporins [H]

COG id: COG2367

COG function: function code V; Beta-lactamase class A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 29922; Mature: 29922

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVRAQPASSVSINLAARERFEQLEASSGGRLGVAALRTADGSYVGYRESERFPMCSTF
CCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHH
KLLAVALILKRSMAERGLLEERIRYGDAQVVAHSPVTKRHAGGEGMTVGELSAAALQHSD
HHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHCCC
NTAANLLLTSLGGPEVLNQFALSIGDTWFDLVRNEPEVNASVPGDMRDTTSPRAMLLDVQ
CHHHHEEEECCCCHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KLLLADDVLGAREREQLKAWMLGNTTGATRIRAAVPAAGWLVADNTGSGDYGTANDVAVV
HHHHHHHHHCCHHHHHHHHHHCCCCCCCHHEEEECCCCCEEEECCCCCCCCCCCCCEEEE
YPPSSAPLVVAIYFTGVTPKETPPQDAIVDEAARIVFDALR
ECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGVRAQPASSVSINLAARERFEQLEASSGGRLGVAALRTADGSYVGYRESERFPMCSTF
CCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHH
KLLAVALILKRSMAERGLLEERIRYGDAQVVAHSPVTKRHAGGEGMTVGELSAAALQHSD
HHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHCCC
NTAANLLLTSLGGPEVLNQFALSIGDTWFDLVRNEPEVNASVPGDMRDTTSPRAMLLDVQ
CHHHHEEEECCCCHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KLLLADDVLGAREREQLKAWMLGNTTGATRIRAAVPAAGWLVADNTGSGDYGTANDVAVV
HHHHHHHHHCCHHHHHHHHHHCCCCCCCHHEEEECCCCCEEEECCCCCCCCCCCCCEEEE
YPPSSAPLVVAIYFTGVTPKETPPQDAIVDEAARIVFDALR
ECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8834913; 1633193 [H]