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The map label for this gene is btuB [H]

Identifier: 186476964

GI number: 186476964

Start: 2486672

End: 2488537

Strand: Reverse

Name: btuB [H]

Synonym: Bphy_2213

Alternate gene names: 186476964

Gene position: 2488537-2486672 (Counterclockwise)

Preceding gene: 186476970

Following gene: 186476963

Centisome position: 71.53

GC content: 63.67

Gene sequence:

>1866_bases
ATGCTGACCCCGCTGTTCGTCCAGCCGGCGCTTCCGCGCCCGCATGCGCCCCCCGCGTTCATCGCGCCGCTCGCCGTCAT
GGCCTTCGCGTGGGTGCCGCTCGCTGTGCACGCGCAGTCGGACGACACCGCGAATCCATCCGCCAAATCCACGGATCCGT
CGGACAACGCGTCGAATCGTCTGCAGGCAGCCGCGTCCTGGTCGAACGATACGGCGACGCTCTCACCCATCGTCGTGACG
GCGGGCCGCGGCGCGCAGCGCCTCGCCGAAGCGATTGCGCAGACTACGCTGTTCGACGCGCAGGACATCGCGGACACCAC
TGCACGCGACTTGCCCGGCTTCTTGCAGTTCGCGCCCGGTGCGCAGATCGTGCGCAACGGCGGCCCTGGATCGACGTCGA
GCCTGTTTCTGCGCGGTGCATCGGCGACGCAGTCGCTGGTGTTGATCGACGGCGTGCGGGTCGATTCGGTGAGTCAGGGC
AATGCGCAGCTCGAACAGATTCCGCTTGATCAGATCGATCACGTCGAAGTCGTGAACGGTAATGTCTCGGCGTTATACGG
GTCGGGTGCGATTGGCGGCGTGGTGCAGGTGTTCACGAAAGACGGCGCCAACCATCCGCCGCGTTTTCATTTCGAGGTCG
AATACGGCAGCTATCACACGCAGCGCGAGGCGGCGGGCATCGACGGCGCACTCGACAAGGACGGCACAACGACCTTCAGC
GTCATGTTCGCGCGCGCGAAGACGGATGGGTTTTCGTCGATCGATCCGGCGCTCGTGCCCGTCAATCCGAACGCCAACGG
CTACTTCGACGAGAGCGTGAGCGCGTCGCTGCGTCACAAGCTCAACGACAGATGGGACATCGGCTTGCGCTACTTCCTGT
CGAACGGCGACGACAGCTTTGACAATCCGTTCGGCCTGCCCGCCGATCTCAATAACGTGCGCAGCAAAGTTCAGACCGCA
TCGGCCTTTGCGAACGGCAAGCTGAGCAACGGGTGGGCCATGCGCTTCATCGTCGCGACGGGCATCGATCGCAGCAACAC
GTCGCTCAATGGCGCCTATCGGTATCGCTACGATTCGGACAACCGGCAATACACGTGGCAGAACGATTTCACGTTTGCGC
GCAATCAGAAGCTGCAAGTCGGCTACGAGCATCTCGATCAGACGCTCGACTCGAACCAGTACAGCGCGCCCGACCGTCAC
GTGAACTCCGTGTTCGCGGGCTACACGGCGCGCTTAGGCGACAGCCAGATCCAGGCGAACCTGCGGCGAGACGAGTACTC
GGATCTCGGCGGAGCCAACAGTTACTATCTCGGCTATGGCTACGACTTCGACGAGCGCTGGAAGGCGACTGCAAGCTGGT
CGGATGCGTTCCGCGCGCCGAGCTTCAACGATCTGTACTACCCGTATAGCGGCAATCCGCTCATCCAGCCGGAGCGCAGT
CATTCCGTCGAAGCGGCGCTGCAATATACGTCGGACGCGCTCGGGCTGATGAAGCTCACTGCGTTCGAGACGCGCTATAC
GAACCTGATCGATTATGTGCAGACGACACCCGGCATCTATGTTGCGGAGAACGTGGGGCAGGCGAAGGTGCAGGGCATCG
AGGGCTCATGGGCGGGCCGTGTCGGCAAGACGGACGTGCGCGCGGCCGTGACGCTCCAGAATCCCGTCGACGAAGACACG
CACACCGATCTCAACCGGCGCGCGCGTCATTTCGCGTCGTTCGCGGCGAACCGCAACATCGGCGGCTGGCGCGTGGGCGG
CGAATGGATCGTGAGCGGCGAGCGCGACGACAGCGGCACGCCGCTCGGCGGATATGGCGTCGTGAACCTTCCGCGCGCTA
CAACATCACGAAGGCCTGGTACGTAG

Upstream 100 bases:

>100_bases
GCCAGCCCGGATACCGGCCAGAGTCGGAGCGCATGACGCTTCCCGAACAGGCATCGGCCCACGAGGAGCGGGCCGCGCAC
AGGCCTACAGGACTCTTTCG

Downstream 100 bases:

>100_bases
CCGCGCAAATCCAGAATCTCTTCGACAAGGACTATGAACTCGCCTACACGTACAACACGCCGCGACGTGGCGCTTACCTC
GCGCTCGGCTGGCAGCAACA

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MLTPLFVQPALPRPHAPPAFIAPLAVMAFAWVPLAVHAQSDDTANPSAKSTDPSDNASNRLQAAASWSNDTATLSPIVVT
AGRGAQRLAEAIAQTTLFDAQDIADTTARDLPGFLQFAPGAQIVRNGGPGSTSSLFLRGASATQSLVLIDGVRVDSVSQG
NAQLEQIPLDQIDHVEVVNGNVSALYGSGAIGGVVQVFTKDGANHPPRFHFEVEYGSYHTQREAAGIDGALDKDGTTTFS
VMFARAKTDGFSSIDPALVPVNPNANGYFDESVSASLRHKLNDRWDIGLRYFLSNGDDSFDNPFGLPADLNNVRSKVQTA
SAFANGKLSNGWAMRFIVATGIDRSNTSLNGAYRYRYDSDNRQYTWQNDFTFARNQKLQVGYEHLDQTLDSNQYSAPDRH
VNSVFAGYTARLGDSQIQANLRRDEYSDLGGANSYYLGYGYDFDERWKATASWSDAFRAPSFNDLYYPYSGNPLIQPERS
HSVEAALQYTSDALGLMKLTAFETRYTNLIDYVQTTPGIYVAENVGQAKVQGIEGSWAGRVGKTDVRAAVTLQNPVDEDT
HTDLNRRARHFASFAANRNIGGWRVGGEWIVSGERDDSGTPLGGYGVVNLPRATTSRRPGT

Sequences:

>Translated_621_residues
MLTPLFVQPALPRPHAPPAFIAPLAVMAFAWVPLAVHAQSDDTANPSAKSTDPSDNASNRLQAAASWSNDTATLSPIVVT
AGRGAQRLAEAIAQTTLFDAQDIADTTARDLPGFLQFAPGAQIVRNGGPGSTSSLFLRGASATQSLVLIDGVRVDSVSQG
NAQLEQIPLDQIDHVEVVNGNVSALYGSGAIGGVVQVFTKDGANHPPRFHFEVEYGSYHTQREAAGIDGALDKDGTTTFS
VMFARAKTDGFSSIDPALVPVNPNANGYFDESVSASLRHKLNDRWDIGLRYFLSNGDDSFDNPFGLPADLNNVRSKVQTA
SAFANGKLSNGWAMRFIVATGIDRSNTSLNGAYRYRYDSDNRQYTWQNDFTFARNQKLQVGYEHLDQTLDSNQYSAPDRH
VNSVFAGYTARLGDSQIQANLRRDEYSDLGGANSYYLGYGYDFDERWKATASWSDAFRAPSFNDLYYPYSGNPLIQPERS
HSVEAALQYTSDALGLMKLTAFETRYTNLIDYVQTTPGIYVAENVGQAKVQGIEGSWAGRVGKTDVRAAVTLQNPVDEDT
HTDLNRRARHFASFAANRNIGGWRVGGEWIVSGERDDSGTPLGGYGVVNLPRATTSRRPGT
>Mature_621_residues
MLTPLFVQPALPRPHAPPAFIAPLAVMAFAWVPLAVHAQSDDTANPSAKSTDPSDNASNRLQAAASWSNDTATLSPIVVT
AGRGAQRLAEAIAQTTLFDAQDIADTTARDLPGFLQFAPGAQIVRNGGPGSTSSLFLRGASATQSLVLIDGVRVDSVSQG
NAQLEQIPLDQIDHVEVVNGNVSALYGSGAIGGVVQVFTKDGANHPPRFHFEVEYGSYHTQREAAGIDGALDKDGTTTFS
VMFARAKTDGFSSIDPALVPVNPNANGYFDESVSASLRHKLNDRWDIGLRYFLSNGDDSFDNPFGLPADLNNVRSKVQTA
SAFANGKLSNGWAMRFIVATGIDRSNTSLNGAYRYRYDSDNRQYTWQNDFTFARNQKLQVGYEHLDQTLDSNQYSAPDRH
VNSVFAGYTARLGDSQIQANLRRDEYSDLGGANSYYLGYGYDFDERWKATASWSDAFRAPSFNDLYYPYSGNPLIQPERS
HSVEAALQYTSDALGLMKLTAFETRYTNLIDYVQTTPGIYVAENVGQAKVQGIEGSWAGRVGKTDVRAAVTLQNPVDEDT
HTDLNRRARHFASFAANRNIGGWRVGGEWIVSGERDDSGTPLGGYGVVNLPRATTSRRPGT

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=533, Percent_Identity=26.266416510319, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI1788478, Length=128, Percent_Identity=35.15625, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67461; Mature: 67461

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLFVQPALPRPHAPPAFIAPLAVMAFAWVPLAVHAQSDDTANPSAKSTDPSDNASNR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCHHH
LQAAASWSNDTATLSPIVVTAGRGAQRLAEAIAQTTLFDAQDIADTTARDLPGFLQFAPG
EEEHCCCCCCCEEECEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHEEECCC
AQIVRNGGPGSTSSLFLRGASATQSLVLIDGVRVDSVSQGNAQLEQIPLDQIDHVEVVNG
HHEEECCCCCCCCEEEEECCCCCCEEEEEECEEECCCCCCCCHHHHCCCCCCCCEEEECC
NVSALYGSGAIGGVVQVFTKDGANHPPRFHFEVEYGSYHTQREAAGIDGALDKDGTTTFS
CEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCEEEE
VMFARAKTDGFSSIDPALVPVNPNANGYFDESVSASLRHKLNDRWDIGLRYFLSNGDDSF
EEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCC
DNPFGLPADLNNVRSKVQTASAFANGKLSNGWAMRFIVATGIDRSNTSLNGAYRYRYDSD
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCC
NRQYTWQNDFTFARNQKLQVGYEHLDQTLDSNQYSAPDRHVNSVFAGYTARLGDSQIQAN
CCEEEECCCCEEECCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
LRRDEYSDLGGANSYYLGYGYDFDERWKATASWSDAFRAPSFNDLYYPYSGNPLIQPERS
HCCCHHHCCCCCCCEEEEECCCHHHHHCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCCC
HSVEAALQYTSDALGLMKLTAFETRYTNLIDYVQTTPGIYVAENVGQAKVQGIEGSWAGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCC
VGKTDVRAAVTLQNPVDEDTHTDLNRRARHFASFAANRNIGGWRVGGEWIVSGERDDSGT
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCCC
PLGGYGVVNLPRATTSRRPGT
CCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MLTPLFVQPALPRPHAPPAFIAPLAVMAFAWVPLAVHAQSDDTANPSAKSTDPSDNASNR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCHHH
LQAAASWSNDTATLSPIVVTAGRGAQRLAEAIAQTTLFDAQDIADTTARDLPGFLQFAPG
EEEHCCCCCCCEEECEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHEEECCC
AQIVRNGGPGSTSSLFLRGASATQSLVLIDGVRVDSVSQGNAQLEQIPLDQIDHVEVVNG
HHEEECCCCCCCCEEEEECCCCCCEEEEEECEEECCCCCCCCHHHHCCCCCCCCEEEECC
NVSALYGSGAIGGVVQVFTKDGANHPPRFHFEVEYGSYHTQREAAGIDGALDKDGTTTFS
CEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCEEEE
VMFARAKTDGFSSIDPALVPVNPNANGYFDESVSASLRHKLNDRWDIGLRYFLSNGDDSF
EEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCC
DNPFGLPADLNNVRSKVQTASAFANGKLSNGWAMRFIVATGIDRSNTSLNGAYRYRYDSD
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCC
NRQYTWQNDFTFARNQKLQVGYEHLDQTLDSNQYSAPDRHVNSVFAGYTARLGDSQIQAN
CCEEEECCCCEEECCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
LRRDEYSDLGGANSYYLGYGYDFDERWKATASWSDAFRAPSFNDLYYPYSGNPLIQPERS
HCCCHHHCCCCCCCEEEEECCCHHHHHCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCCC
HSVEAALQYTSDALGLMKLTAFETRYTNLIDYVQTTPGIYVAENVGQAKVQGIEGSWAGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCC
VGKTDVRAAVTLQNPVDEDTHTDLNRRARHFASFAANRNIGGWRVGGEWIVSGERDDSGT
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCCC
PLGGYGVVNLPRATTSRRPGT
CCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA