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The map label for this gene is hemC

Identifier: 186476930

GI number: 186476930

Start: 2443380

End: 2444363

Strand: Reverse

Name: hemC

Synonym: Bphy_2179

Alternate gene names: 186476930

Gene position: 2444363-2443380 (Counterclockwise)

Preceding gene: 186476932

Following gene: 186476929

Centisome position: 70.26

GC content: 67.48

Gene sequence:

>984_bases
ATGAATTCCGAGACGCTTGCGCCCACGCCGCCCGCCACGCTTGTGATCGCGTCGCGTGAAAGCCGGCTTGCAATGTGGCA
GGCCGAGCACGTGCGGTGTGCGCTGCACAAATTATATCCATCTTGTGACGTAAAAATCCTCGGAATGACGACTCGCGGCG
ATCAGATTCTCGATCGCACGCTGTCGAAGGTCGGCGGCAAAGGGTTGTTCGTGAAGGAACTGGAGAACGCGCTCGCCGAC
GGTCGCGCCGATCTCGCCGTTCATTCGCTCAAAGACGTGCCCATGGAACTGCCCGAGGGCTTCGTGCTGTCGACCATCAT
GGAGCGCGAAGATCCGCGCGACGCGTTTGTCTCGAACCAGTACGACTCGCTGGCTGCGCTGCCGGCCGGCAGCGTGGTCG
GCACGTCGAGCCTGCGCCGCGAAGCGATGCTGCGCGCGCGTTATCCCGAGCTTGTCGTGAAGCCGCTGCGCGGCAATCTG
GACACGCGGCTTGGCAAACTCGACCGCGGCGACTATGCGGCCATCATTCTCGCTGCTGCGGGCCTCAAGCGCCTCGGCCT
CGGCGAGCGCATCCGTTCGCTGCTCGATCCCGCGGACAGCCTGCCCGCTGCGGGTCAAGGCGCGCTCGGCATCGAGATTC
GCGCCGGCCGCGACGATCTTGCTGCATGGCTCGCGCCGCTGCATCACGAGCACACGGCGGCTGCCGTCGAAGCAGAGCGC
ATGGTGTCGCGCACGCTGGGCGGCAGTTGCGAAGTGCCCCTCGCCGCGTATGCGACATGGCACGACGGCGCGCTGCATTT
GCGCGGCATCGTCGCCACGCCGGACGGCGAGCGGGTGCTGAGCGCGCAGGCGTCGGCGCCTGCAGCCACGACGGACGCCG
CGCTCGAACTGGGTCGCGAGGTCGCCAGCCAGCTAGAGGCGCAAGGCGCGCTCGACATTGTGCGCGCGCTCTCCACCGCA
AGCGGCCCGGCCGCTTCCGCGTGA

Upstream 100 bases:

>100_bases
TGTCGAACTGCTTCGGCATGGCCGTGCGGCCAGCGAGAGGTTGCCGAAGCGCGTGCTAACATTGTTTGTTATCACATCCC
GTCGTTCAATGACTCAAACA

Downstream 100 bases:

>100_bases
TGGCGGCCAGCTCGAATCCGACACCCGGTGCATTCCCGTCCAAGGACGGCGCAGGCTCGCCGCATGCGTCGGGCGCGCGC
CTGACGGTCGTGCTGACCCG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNSETLAPTPPATLVIASRESRLAMWQAEHVRCALHKLYPSCDVKILGMTTRGDQILDRTLSKVGGKGLFVKELENALAD
GRADLAVHSLKDVPMELPEGFVLSTIMEREDPRDAFVSNQYDSLAALPAGSVVGTSSLRREAMLRARYPELVVKPLRGNL
DTRLGKLDRGDYAAIILAAAGLKRLGLGERIRSLLDPADSLPAAGQGALGIEIRAGRDDLAAWLAPLHHEHTAAAVEAER
MVSRTLGGSCEVPLAAYATWHDGALHLRGIVATPDGERVLSAQASAPAATTDAALELGREVASQLEAQGALDIVRALSTA
SGPAASA

Sequences:

>Translated_327_residues
MNSETLAPTPPATLVIASRESRLAMWQAEHVRCALHKLYPSCDVKILGMTTRGDQILDRTLSKVGGKGLFVKELENALAD
GRADLAVHSLKDVPMELPEGFVLSTIMEREDPRDAFVSNQYDSLAALPAGSVVGTSSLRREAMLRARYPELVVKPLRGNL
DTRLGKLDRGDYAAIILAAAGLKRLGLGERIRSLLDPADSLPAAGQGALGIEIRAGRDDLAAWLAPLHHEHTAAAVEAER
MVSRTLGGSCEVPLAAYATWHDGALHLRGIVATPDGERVLSAQASAPAATTDAALELGREVASQLEAQGALDIVRALSTA
SGPAASA
>Mature_327_residues
MNSETLAPTPPATLVIASRESRLAMWQAEHVRCALHKLYPSCDVKILGMTTRGDQILDRTLSKVGGKGLFVKELENALAD
GRADLAVHSLKDVPMELPEGFVLSTIMEREDPRDAFVSNQYDSLAALPAGSVVGTSSLRREAMLRARYPELVVKPLRGNL
DTRLGKLDRGDYAAIILAAAGLKRLGLGERIRSLLDPADSLPAAGQGALGIEIRAGRDDLAAWLAPLHHEHTAAAVEAER
MVSRTLGGSCEVPLAAYATWHDGALHLRGIVATPDGERVLSAQASAPAATTDAALELGREVASQLEAQGALDIVRALSTA
SGPAASA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=338, Percent_Identity=41.1242603550296, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI66933009, Length=338, Percent_Identity=41.1242603550296, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI48994974, Length=312, Percent_Identity=53.525641025641, Blast_Score=306, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6319996, Length=320, Percent_Identity=38.75, Blast_Score=200, Evalue=2e-52,
Organism=Drosophila melanogaster, GI20130425, Length=249, Percent_Identity=47.3895582329317, Blast_Score=220, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_BURP8 (B2JEN9)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858400.1
- GeneID:   6243665
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2179
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34552; Mature: 34552

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSETLAPTPPATLVIASRESRLAMWQAEHVRCALHKLYPSCDVKILGMTTRGDQILDRT
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
LSKVGGKGLFVKELENALADGRADLAVHSLKDVPMELPEGFVLSTIMEREDPRDAFVSNQ
HHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCHHHHHCCC
YDSLAALPAGSVVGTSSLRREAMLRARYPELVVKPLRGNLDTRLGKLDRGDYAAIILAAA
CHHHHHCCCCCHHCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHH
GLKRLGLGERIRSLLDPADSLPAAGQGALGIEIRAGRDDLAAWLAPLHHEHTAAAVEAER
HHHHCCHHHHHHHHHCCHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHH
MVSRTLGGSCEVPLAAYATWHDGALHLRGIVATPDGERVLSAQASAPAATTDAALELGRE
HHHHHCCCCCCCCHHEEEEECCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHH
VASQLEAQGALDIVRALSTASGPAASA
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNSETLAPTPPATLVIASRESRLAMWQAEHVRCALHKLYPSCDVKILGMTTRGDQILDRT
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
LSKVGGKGLFVKELENALADGRADLAVHSLKDVPMELPEGFVLSTIMEREDPRDAFVSNQ
HHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCHHHHHCCC
YDSLAALPAGSVVGTSSLRREAMLRARYPELVVKPLRGNLDTRLGKLDRGDYAAIILAAA
CHHHHHCCCCCHHCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHH
GLKRLGLGERIRSLLDPADSLPAAGQGALGIEIRAGRDDLAAWLAPLHHEHTAAAVEAER
HHHHCCHHHHHHHHHCCHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHH
MVSRTLGGSCEVPLAAYATWHDGALHLRGIVATPDGERVLSAQASAPAATTDAALELGRE
HHHHHCCCCCCCCHHEEEEECCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHH
VASQLEAQGALDIVRALSTASGPAASA
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA