Click here to switch to the map view.

The map label for this gene is hemD [H]

Identifier: 186476929

GI number: 186476929

Start: 2441320

End: 2443380

Strand: Reverse

Name: hemD [H]

Synonym: Bphy_2178

Alternate gene names: 186476929

Gene position: 2443380-2441320 (Counterclockwise)

Preceding gene: 186476930

Following gene: 186476928

Centisome position: 70.23

GC content: 67.01

Gene sequence:

>2061_bases
ATGGCGGCCAGCTCGAATCCGACACCCGGTGCATTCCCGTCCAAGGACGGCGCAGGCTCGCCGCATGCGTCGGGCGCGCG
CCTGACGGTCGTGCTGACCCGCCCGGCGGGGCAGTCCGAGGCGCTCGCGAGGACGCTGCATGATGCCGGTGTCGCAACCG
TCGAATTCCCCCTCATCGAGATCGCCGCAACCGACGACGCAACGCCGCTGCGTGCGGCGCTCGCGTCGCTGGAAAAGTAC
GCGCTCGTGGTGTTCGTGTCGCCCAATGCCGTCGACTACGCATTCGCAAACCACGACGCGATCTGGCCGCATGCGCTGCC
TATCGGCGTGGTCGGGCCTGGTAGCGTCGCGGCGCTCGCGAAGCACGGCGTCGAGGCGCCCGCGTACCAGGTGATCAGCC
CCGGCGCAGCCGCCGACGACAACGGCGACGGCGCACGCTTCGATTCAGAGGCGTTGTATTCCGCGCTGGAGGCGTCGCTC
GGCGACAACGCGTTCGAAGGCAAGCGTGTGCTGATCGTGCGTGGCGACGGCGGGCGCGAATGGCTCGCCGAACGTCTGCG
CGAAGCGGGCGCGGAGGTCGAGACGGTCGCGGCCTACCGGCGCCTCGTGCCCGAGCCGTCGATCGGGGCGTGGTCGCGCA
TTCACGCGCTGCTGTCGGACACATCGACTGCGCGGCACGCGTGGCTCCTGACGAGTTCCGAAGGCGCGCGCAATCTCGCC
GAACTCGCGCACGACCATCTGACGAACAGCGAGCAGACCCATCTGAAGCGCGCGATGTTCGTCGCGCCCCACCCGCGCAT
CGCGGAAACCGCGCGGGCATTGGGTTTTGATAGCATTACGGTGTCCGGTGCGGGCGATGAGCGCATTGCCCGCACGTTGC
TGTCTCTCGCGACACCGCCCGTAGTTCAAGCGGTCCAGTCCAACCCGGCACAAGAACGCATGTCTGATACCAACGCATCC
ACGACCCCCACGCCTAATCCCGCGCCGCGGCCCGCGAACACGCTGCCGCCGAATCAGCCCTTTACGCCCTACGAACTGCA
GCAGCCCAACCGTAAAGGCAACGGACTCGTCTGGGTGGTCATCGCGCTGGTGCTCGCCGCGGCGGTCGCGGGCGGCCTTG
CGCTGAACCGCAGAATCATCAAGCTCGACCAGCAGTTGTCGCACCGGCAGCAGGCCAACGACGCCCAGATGGCCGAATTG
CGCGTGAAGACGGATCAGGCCGTCTCGACCGTGCGTCAGGCCGACGCGCAGATCGCGCAGCTCGAAGGCAAGCTCGCCGA
TGCGCAGAACGCGCAGCAGGCGCTTCAGCAACAATACGCAGACCTCGCACGCAACCGCGACGACTGGACGATGGCCGAAG
TCGGCCAGATGCTTTCGGCGGCGAGCGAGCAACTGCAATTGACGGGTAACACGCAGCTCGCGCTGTTCGCGCTGCAAAGC
GCCGACACGCGCCTCGCCGCGTCGGATGGCGCGCAGGTGCTGGCCGTGCGCAAGGCGATCGCGCAGGACATCGACAGGCT
GAAGTCCGCGCCGTCCACCGACCTGACAGGCCTCGCCATCAAGCTCGATAACGCCATCGATCAGATCGACCAGCTGCCGC
TGTCGGGCGAGGCGCCGATCGCGCACGCTACGCCGCACGCGTCGACGCCGAAGGACGTCGACCACGTCGCGGCGGCGACG
GGCGAGCCGAAGTGGAAGGTGTGGTGGAACCAGTTCGTCGCGGGTGTCGGCGAGCAGTTGACCAGCCTCGTGCAGGTGCG
CCGCATCGATCACGCCGACGCGATGCTCGTCGCGCCCGATCAGGGTTATTTCGTGCGTGAGAATCTGAAGCTGCGTCTGC
TGTCCGCGCGTCTCGCGCTGCTCGCACGCAACCAGACAACGCTCAAATCGGATCTTCATGCGGCGGATAGCACGATTGGG
CGCTACTTCGACGCGTCGTCGAAGAGCGTGCAGACGGTGCGCGACCTCATCAAGCAGATCGACGAAGGCTCGGGCGCCGT
CGACGTTCCGAACCTGAATACGAGCCTGCAGGCCGTGCATCAATATCGGAACCGGGGTTAA

Upstream 100 bases:

>100_bases
TCGAACTGGGTCGCGAGGTCGCCAGCCAGCTAGAGGCGCAAGGCGCGCTCGACATTGTGCGCGCGCTCTCCACCGCAAGC
GGCCCGGCCGCTTCCGCGTG

Downstream 100 bases:

>100_bases
TCATGGCGCTGCGTGGACTTCTCTGGCTCGCATTCCTGTTCGCGATCGCCGTCGTGCTCGCGATCGTCGGGCGCTTCGAC
ACGGGGCAGGTGCTGCTCGT

Product: bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MAASSNPTPGAFPSKDGAGSPHASGARLTVVLTRPAGQSEALARTLHDAGVATVEFPLIEIAATDDATPLRAALASLEKY
ALVVFVSPNAVDYAFANHDAIWPHALPIGVVGPGSVAALAKHGVEAPAYQVISPGAAADDNGDGARFDSEALYSALEASL
GDNAFEGKRVLIVRGDGGREWLAERLREAGAEVETVAAYRRLVPEPSIGAWSRIHALLSDTSTARHAWLLTSSEGARNLA
ELAHDHLTNSEQTHLKRAMFVAPHPRIAETARALGFDSITVSGAGDERIARTLLSLATPPVVQAVQSNPAQERMSDTNAS
TTPTPNPAPRPANTLPPNQPFTPYELQQPNRKGNGLVWVVIALVLAAAVAGGLALNRRIIKLDQQLSHRQQANDAQMAEL
RVKTDQAVSTVRQADAQIAQLEGKLADAQNAQQALQQQYADLARNRDDWTMAEVGQMLSAASEQLQLTGNTQLALFALQS
ADTRLAASDGAQVLAVRKAIAQDIDRLKSAPSTDLTGLAIKLDNAIDQIDQLPLSGEAPIAHATPHASTPKDVDHVAAAT
GEPKWKVWWNQFVAGVGEQLTSLVQVRRIDHADAMLVAPDQGYFVRENLKLRLLSARLALLARNQTTLKSDLHAADSTIG
RYFDASSKSVQTVRDLIKQIDEGSGAVDVPNLNTSLQAVHQYRNRG

Sequences:

>Translated_686_residues
MAASSNPTPGAFPSKDGAGSPHASGARLTVVLTRPAGQSEALARTLHDAGVATVEFPLIEIAATDDATPLRAALASLEKY
ALVVFVSPNAVDYAFANHDAIWPHALPIGVVGPGSVAALAKHGVEAPAYQVISPGAAADDNGDGARFDSEALYSALEASL
GDNAFEGKRVLIVRGDGGREWLAERLREAGAEVETVAAYRRLVPEPSIGAWSRIHALLSDTSTARHAWLLTSSEGARNLA
ELAHDHLTNSEQTHLKRAMFVAPHPRIAETARALGFDSITVSGAGDERIARTLLSLATPPVVQAVQSNPAQERMSDTNAS
TTPTPNPAPRPANTLPPNQPFTPYELQQPNRKGNGLVWVVIALVLAAAVAGGLALNRRIIKLDQQLSHRQQANDAQMAEL
RVKTDQAVSTVRQADAQIAQLEGKLADAQNAQQALQQQYADLARNRDDWTMAEVGQMLSAASEQLQLTGNTQLALFALQS
ADTRLAASDGAQVLAVRKAIAQDIDRLKSAPSTDLTGLAIKLDNAIDQIDQLPLSGEAPIAHATPHASTPKDVDHVAAAT
GEPKWKVWWNQFVAGVGEQLTSLVQVRRIDHADAMLVAPDQGYFVRENLKLRLLSARLALLARNQTTLKSDLHAADSTIG
RYFDASSKSVQTVRDLIKQIDEGSGAVDVPNLNTSLQAVHQYRNRG
>Mature_685_residues
AASSNPTPGAFPSKDGAGSPHASGARLTVVLTRPAGQSEALARTLHDAGVATVEFPLIEIAATDDATPLRAALASLEKYA
LVVFVSPNAVDYAFANHDAIWPHALPIGVVGPGSVAALAKHGVEAPAYQVISPGAAADDNGDGARFDSEALYSALEASLG
DNAFEGKRVLIVRGDGGREWLAERLREAGAEVETVAAYRRLVPEPSIGAWSRIHALLSDTSTARHAWLLTSSEGARNLAE
LAHDHLTNSEQTHLKRAMFVAPHPRIAETARALGFDSITVSGAGDERIARTLLSLATPPVVQAVQSNPAQERMSDTNAST
TPTPNPAPRPANTLPPNQPFTPYELQQPNRKGNGLVWVVIALVLAAAVAGGLALNRRIIKLDQQLSHRQQANDAQMAELR
VKTDQAVSTVRQADAQIAQLEGKLADAQNAQQALQQQYADLARNRDDWTMAEVGQMLSAASEQLQLTGNTQLALFALQSA
DTRLAASDGAQVLAVRKAIAQDIDRLKSAPSTDLTGLAIKLDNAIDQIDQLPLSGEAPIAHATPHASTPKDVDHVAAATG
EPKWKVWWNQFVAGVGEQLTSLVQVRRIDHADAMLVAPDQGYFVRENLKLRLLSARLALLARNQTTLKSDLHAADSTIGR
YFDASSKSVQTVRDLIKQIDEGSGAVDVPNLNTSLQAVHQYRNRG

Specific function: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen-III synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790235, Length=313, Percent_Identity=24.9201277955272, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790236, Length=270, Percent_Identity=27.4074074074074, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003754 [H]

Pfam domain/function: PF02602 HEM4 [H]

EC number: =4.2.1.75 [H]

Molecular weight: Translated: 73064; Mature: 72932

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASSNPTPGAFPSKDGAGSPHASGARLTVVLTRPAGQSEALARTLHDAGVATVEFPLIE
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECEEE
IAATDDATPLRAALASLEKYALVVFVSPNAVDYAFANHDAIWPHALPIGVVGPGSVAALA
EEECCCCCHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCCCCCCEECEECCCHHHHHH
KHGVEAPAYQVISPGAAADDNGDGARFDSEALYSALEASLGDNAFEGKRVLIVRGDGGRE
HCCCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHH
WLAERLREAGAEVETVAAYRRLVPEPSIGAWSRIHALLSDTSTARHAWLLTSSEGARNLA
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
ELAHDHLTNSEQTHLKRAMFVAPHPRIAETARALGFDSITVSGAGDERIARTLLSLATPP
HHHHHHCCCCHHHHHHHHHEECCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCH
VVQAVQSNPAQERMSDTNASTTPTPNPAPRPANTLPPNQPFTPYELQQPNRKGNGLVWVV
HHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
IALVLAAAVAGGLALNRRIIKLDQQLSHRQQANDAQMAELRVKTDQAVSTVRQADAQIAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEHHHHHHHHHHHHHHHHHH
LEGKLADAQNAQQALQQQYADLARNRDDWTMAEVGQMLSAASEQLQLTGNTQLALFALQS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEECC
ADTRLAASDGAQVLAVRKAIAQDIDRLKSAPSTDLTGLAIKLDNAIDQIDQLPLSGEAPI
CCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCC
AHATPHASTPKDVDHVAAATGEPKWKVWWNQFVAGVGEQLTSLVQVRRIDHADAMLVAPD
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
QGYFVRENLKLRLLSARLALLARNQTTLKSDLHAADSTIGRYFDASSKSVQTVRDLIKQI
CCCEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEGSGAVDVPNLNTSLQAVHQYRNRG
HCCCCCEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AASSNPTPGAFPSKDGAGSPHASGARLTVVLTRPAGQSEALARTLHDAGVATVEFPLIE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECEEE
IAATDDATPLRAALASLEKYALVVFVSPNAVDYAFANHDAIWPHALPIGVVGPGSVAALA
EEECCCCCHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCCCCCCEECEECCCHHHHHH
KHGVEAPAYQVISPGAAADDNGDGARFDSEALYSALEASLGDNAFEGKRVLIVRGDGGRE
HCCCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHH
WLAERLREAGAEVETVAAYRRLVPEPSIGAWSRIHALLSDTSTARHAWLLTSSEGARNLA
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
ELAHDHLTNSEQTHLKRAMFVAPHPRIAETARALGFDSITVSGAGDERIARTLLSLATPP
HHHHHHCCCCHHHHHHHHHEECCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCH
VVQAVQSNPAQERMSDTNASTTPTPNPAPRPANTLPPNQPFTPYELQQPNRKGNGLVWVV
HHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
IALVLAAAVAGGLALNRRIIKLDQQLSHRQQANDAQMAELRVKTDQAVSTVRQADAQIAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEHHHHHHHHHHHHHHHHHH
LEGKLADAQNAQQALQQQYADLARNRDDWTMAEVGQMLSAASEQLQLTGNTQLALFALQS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEECC
ADTRLAASDGAQVLAVRKAIAQDIDRLKSAPSTDLTGLAIKLDNAIDQIDQLPLSGEAPI
CCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCC
AHATPHASTPKDVDHVAAATGEPKWKVWWNQFVAGVGEQLTSLVQVRRIDHADAMLVAPD
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
QGYFVRENLKLRLLSARLALLARNQTTLKSDLHAADSTIGRYFDASSKSVQTVRDLIKQI
CCCEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEGSGAVDVPNLNTSLQAVHQYRNRG
HCCCCCEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8159168; 10984043 [H]