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The map label for this gene is pnp [H]

Identifier: 186476768

GI number: 186476768

Start: 2263257

End: 2265395

Strand: Reverse

Name: pnp [H]

Synonym: Bphy_2013

Alternate gene names: 186476768

Gene position: 2265395-2263257 (Counterclockwise)

Preceding gene: 186476769

Following gene: 186476767

Centisome position: 65.11

GC content: 63.25

Gene sequence:

>2139_bases
ATGTCTCTGTTTAACAAGGTCGTCAAAGAGTTCAAGTGGGGCCAGCATACGGTTCGCATGGAAACGGGCGAAATCGCCCG
CCAGGCGAGCGGTGCCGTGCTCGTCGACGTCGAAGACACGGTGGTGCTGGCCACCGTCGTCGGTGCGAAGACGGCCAAGC
CGGGTCAGGACTTCTTTCCGCTGACCGTCGATTACATCGAAAAGACCTACTCGGCGGGCAAGATCCCCGGTGGCTTCTTC
CGCCGCGAAGGCCGCCCGTCGGAAGGCGAAACGCTGATCTCGCGACTGATCGACCGTCCGCTGCGTCCGCTCTTCCCGGA
AGGCTTCTACAACGAAGTGCAGGTCGTCATCCACGTGATGTCGATCAACCCTGAAGTCCCCGCGGACATCCCCGCGCTGA
TCGGTGCGTCGGCGGCGCTCGCTGTGTCGGGACTGCCGTTCAACGGCCCCGTCGGCGCTGCGCGCGTTGCTTACATCAAC
AACGAGTACGTGCTGAACCCGACCCGCTCGCAGATCAAGGGCTCGCGTCTGGACCTCGTCGTCGCCGGTACGGAGCGCGC
AGTGCTGATGGTCGAATCCGAGGCGGATCAACTGCCGGAAGACGTGATGCTTGGCGCTGTCGTGTTCGGTCACGAGCAGA
TGCAAACGGCCATCGACGCGATCCACGAACTGGTGCGTGAAGGCGGCAAGGCTGAGTGGGAGTGGCAGGCTGCGCCGAAG
AACGAAGCGCTGATCTCGCGCGTCAACGAAATCGCCTACAACGAACTGCTGTCGGCGTACCAGACGCGCGACAAGCAGGC
TCGCTCGACGAAGCTGAAGGAAGTGTACGCTGCGACGCAAGCCAAGCTCGAAGAAGAAGCGGCAGCAGCGGGCACGGTGG
CAGCCGACAAGGCGTCGGTGGGCAACGTGCTGTTCGACATCGAAGCGAAGATCGTCCGTTCGCAGATCCTGAACGGCGAG
CCGCGTATCGACGGCCGCGACACGCGCACGGTGCGCCCCATCGAAATCCGCACGGGCGTCCTGCCGCGTACGCACGGCTC
GGCGCTCTTCACGCGCGGCGAAACGCAGGCGCTCGTCGTCGCGACGCTCGGCACCAAGGGCGATGAGCAGATCATCGACG
CGCTCGAAGGCGAGTACCGCGACCGTTTCATGCTCCACTACAACATGCCGCCATTCGCGACGGGCGAAACGGGCCGCGTC
GGCTCGCCGAAGCGCCGCGAAATCGGTCACGGCCGTCTGGCGAAGCGCGCGCTGGCCGCGTGCCTGCCGAGCGCCGAAGA
GTTCGGCTACTCGATCCGCGTCGTGTCGGAAATCACCGAATCGAATGGCTCGTCGTCGATGGCATCGGTGTGCGGCGGCT
GTCTCGCGCTGATGGACGCAGGCGTGCCGATGAAGGCGCACGTCGCGGGCATCGCAATGGGGCTGATCCTCGAAGGCAAC
AAGTTCGCGGTGCTGACGGACATCCTCGGCGACGAAGATCATCTCGGCGATATGGACTTCAAGGTGGCGGGCACGGCTGA
CGGCGTGACGGCACTGCAGATGGACATCAAGATCCAGGGCATCACAAAGGAAATCATGCAGGTCGCGCTGGCACAGGCGA
AGGAAGGCCGCATGCACATCCTCGGCAAGATGACCTCGGCTGTGTCCGGCGTAAACACGGAACTGTCGGCGTACGCGCCG
CGCATGATCACCATCAAGATCAACCCGGAAAAGATCCGGGACGTGATCGGCAAGGGCGGTTCGGTGATCCGCGCGCTGAC
GGAAGAAACGGGCACGACGATCGACATCTCCGACGACGGCGTCGTCACGATCGCGAGCACGAGCTCGGAAGGCATGGCGG
AAGCCAAGAAGCGGATCGAGAACATCACGGCGGAAGTCGAAGTCGGTCAGGTTTACGAAGGCACGGTGCTCAAGCTGCTG
GACTTCGGCGCGATCGTCAACCTGCTGCCGGGCAAGGACGGTCTGCTGCACATCTCCGAAATCGCCAACGAGCGCATCAA
GGACATCAATGACTACCTGAAGGAAGGCCAGCAAGTGAAGGTGAAGGTCATTCAGACGGACGAGAAGGGTCGCGTGCGCC
TGTCGGCGAAAGCGCTCCTGAACGAAGCAGCACAGACGGAGCCGACGCCGCAGCAGTAA

Upstream 100 bases:

>100_bases
ATCCTCTGGAATGGCATAACACTCCTCTACCCGAAAGGCTCCGGTCCTGACGATGCCGCGTGTGGTCAGGCGCGGCATGA
CCAAAGATCAAGGAGGACAA

Downstream 100 bases:

>100_bases
CGCGGTGAAAGCGGGACGGCCGGTAGCGCAAAAGCGCGCCGGCCGTTTTTCATGTAGGGTGGATTGCAGTGCGAACCGGT
TTTTGTCTGACCGGTTTGCA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 712; Mature: 711

Protein sequence:

>712_residues
MSLFNKVVKEFKWGQHTVRMETGEIARQASGAVLVDVEDTVVLATVVGAKTAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVMSINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYIN
NEYVLNPTRSQIKGSRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKAEWEWQAAPK
NEALISRVNEIAYNELLSAYQTRDKQARSTKLKEVYAATQAKLEEEAAAAGTVAADKASVGNVLFDIEAKIVRSQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALAACLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMTSAVSGVNTELSAYAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITAEVEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIANERIKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAQTEPTPQQ

Sequences:

>Translated_712_residues
MSLFNKVVKEFKWGQHTVRMETGEIARQASGAVLVDVEDTVVLATVVGAKTAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVMSINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYIN
NEYVLNPTRSQIKGSRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKAEWEWQAAPK
NEALISRVNEIAYNELLSAYQTRDKQARSTKLKEVYAATQAKLEEEAAAAGTVAADKASVGNVLFDIEAKIVRSQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALAACLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMTSAVSGVNTELSAYAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITAEVEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIANERIKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAQTEPTPQQ
>Mature_711_residues
SLFNKVVKEFKWGQHTVRMETGEIARQASGAVLVDVEDTVVLATVVGAKTAKPGQDFFPLTVDYIEKTYSAGKIPGGFFR
REGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVMSINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYINN
EYVLNPTRSQIKGSRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKAEWEWQAAPKN
EALISRVNEIAYNELLSAYQTRDKQARSTKLKEVYAATQAKLEEEAAAAGTVAADKASVGNVLFDIEAKIVRSQILNGEP
RIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVG
SPKRREIGHGRLAKRALAACLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGNK
FAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMTSAVSGVNTELSAYAPR
MITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITAEVEVGQVYEGTVLKLLD
FGAIVNLLPGKDGLLHISEIANERIKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAQTEPTPQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=37.5698324022346, Blast_Score=435, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=64.1726618705036, Blast_Score=919, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=729, Percent_Identity=32.3731138545953, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=36.4022662889518, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=36.4022662889518, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=36.4022662889518, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.3774733637747, Blast_Score=374, Evalue=1e-103,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76765; Mature: 76634

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFNKVVKEFKWGQHTVRMETGEIARQASGAVLVDVEDTVVLATVVGAKTAKPGQDFFP
CCHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVM
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEE
SINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYINNEYVLNPTRSQIKGSRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHCCCCEEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKAEWEWQAAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
NEALISRVNEIAYNELLSAYQTRDKQARSTKLKEVYAATQAKLEEEAAAAGTVAADKASV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GNVLFDIEAKIVRSQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEHHHHHHHHHHCCCCCCCCCCCCCEECEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALAA
EEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCHHHCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEE
LGKMTSAVSGVNTELSAYAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIENITAEVEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHH
IANERIKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAQTEPTPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLFNKVVKEFKWGQHTVRMETGEIARQASGAVLVDVEDTVVLATVVGAKTAKPGQDFFP
CHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVM
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEE
SINPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYINNEYVLNPTRSQIKGSRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHCCCCEEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKAEWEWQAAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
NEALISRVNEIAYNELLSAYQTRDKQARSTKLKEVYAATQAKLEEEAAAAGTVAADKASV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GNVLFDIEAKIVRSQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEHHHHHHHHHHCCCCCCCCCCCCCEECEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALAA
EEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCHHHCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEE
LGKMTSAVSGVNTELSAYAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIENITAEVEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHH
IANERIKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAQTEPTPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA