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The map label for this gene is nuoF2 [H]

Identifier: 186476759

GI number: 186476759

Start: 2255561

End: 2256889

Strand: Reverse

Name: nuoF2 [H]

Synonym: Bphy_2004

Alternate gene names: 186476759

Gene position: 2256889-2255561 (Counterclockwise)

Preceding gene: 186476760

Following gene: 186476758

Centisome position: 64.87

GC content: 61.7

Gene sequence:

>1329_bases
ATGACGTCTTTACACGATCGTCACATCAAGCCGCTGATTCTCGCCGGCCTCAACGGCGACAACTGGCATCTTCAGGACTA
CGTCGAGCGCGGCGGCTACAAGCAGCTGCGCCGCATTCTCGAAGAAAAGATTCCGCCCGAGCAGGTAATCGCCGACGTGA
AGGCGTCGGGGCTGCGCGGCCGTGGCGGTGCGGGCTTTCCGACGGGGCTCAAGTGGAGCTTCATGCCGCGTCAGTTTCCT
GGGCAGAAGTACCTCGTCTGCAATTCGGACGAAGGCGAACCGGGTACGTTCAAGGATCGCGACATCCTGCGCTGGAATCC
GCATGCGCTGATCGAAGGCATGGCGATCGGCGCCTACGCGATGGGCATCACCGTCGGCTACAACTACATCCACGGCGAAA
TCTTCGAAGTCTATCGACGCTTCGAAGCCGCACTGGAAGAAGCGCGCACAGCGGGTTTCCTCGGCGACAACATCATGGGC
TCGGGCTTCTCGTTCCAACTGCATGCGCACCACGGTTATGGCGCATACATCTGCGGCGAAGAAACGGCGCTGCTCGAATC
GCTGGAAGGCAAGAAGGGTCAGCCGCGCTTCAAACCGCCTTTCCCGGCGAGCTTCGGCGTGTACGGCAAGCCGACCACGA
TCAACAACACGGAAACGTTCGCAGCCGTGCCGTTTCTGCTGGCCGTCGGTCCGCAGAACTACCTCGAAATGGGCAAGCCG
AACAACGGCGGCACGAAGATTTTCTCGGTGTCGGGCGACGTCGAGCGTCCGGGCAACTATGAAATTCCGCTCGGCACGCC
GTTCGCCACCCTGATGGAACTGGCGGGCGGCATGCGCGGCGGCAAGAAGATCAAGGCCGTGATTCCTGGCGGTTCGTCGG
CCCCGGTCATTCCGGGCGACATCATGATGCAAACCGACATGGACTACGATTCGATCGCGAAGCAGGGCTCGATGTTGGGC
TCGGGCGCGGTCATCGTGATGGACGAGACGCGCTGCATGGTTCGTTCGCTGCTGCGCCTGTCGTACTTCTATTACGAAGA
GTCGTGCGGTCAGTGCACGCCGTGCCGTGAAGGCACAGGCTGGCTGTATCGCGTCGTGCATCGCATCGAGCATGGTCTGG
GCCGCAAGGAAGACCTGGACCTGCTGAACTCGGTCGCCGAAAACATCATGGGCCGCACGATCTGCGCGCTCGGCGATGCG
GCTGCGATGCCGGTGCGAGGCATGCTCAAGCACTACTGGGACGAATTCGAGTATCACGTCGCCAACAAGCGTTGCCTCAC
GGGCGGTCATGCCGACGCGGCGGCGGCCTCTGAAACGGTCGCTGCATAA

Upstream 100 bases:

>100_bases
GCTCGTGCGGTGACGCCCCCGTGATGCTCGTGAACAACCATCGTATGTGCAGCTTCATGAGCCGCGAGAAGATCGACCAG
CTCCTCGAGGAACTTTCGAA

Downstream 100 bases:

>100_bases
AGCTGAGGCAGACGCGGGCGCTGCTGCGTCGCGATGAGCGAAGCAGCGAGCGGGCGAACGATTGAGCGGTAACAGGTTAA
GGAAGATTGACCATCATGGT

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTSLHDRHIKPLILAGLNGDNWHLQDYVERGGYKQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFP
GQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYAMGITVGYNYIHGEIFEVYRRFEAALEEARTAGFLGDNIMG
SGFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLEMGKP
NNGGTKIFSVSGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGDIMMQTDMDYDSIAKQGSMLG
SGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVHRIEHGLGRKEDLDLLNSVAENIMGRTICALGDA
AAMPVRGMLKHYWDEFEYHVANKRCLTGGHADAAAASETVAA

Sequences:

>Translated_442_residues
MTSLHDRHIKPLILAGLNGDNWHLQDYVERGGYKQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFP
GQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYAMGITVGYNYIHGEIFEVYRRFEAALEEARTAGFLGDNIMG
SGFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLEMGKP
NNGGTKIFSVSGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGDIMMQTDMDYDSIAKQGSMLG
SGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVHRIEHGLGRKEDLDLLNSVAENIMGRTICALGDA
AAMPVRGMLKHYWDEFEYHVANKRCLTGGHADAAAASETVAA
>Mature_441_residues
TSLHDRHIKPLILAGLNGDNWHLQDYVERGGYKQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFPG
QKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYAMGITVGYNYIHGEIFEVYRRFEAALEEARTAGFLGDNIMGS
GFSFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLEMGKPN
NGGTKIFSVSGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGDIMMQTDMDYDSIAKQGSMLGS
GAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVHRIEHGLGRKEDLDLLNSVAENIMGRTICALGDAA
AMPVRGMLKHYWDEFEYHVANKRCLTGGHADAAAASETVAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=420, Percent_Identity=46.6666666666667, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI260656005, Length=420, Percent_Identity=46.6666666666667, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1788620, Length=397, Percent_Identity=43.3249370277078, Blast_Score=333, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17531727, Length=424, Percent_Identity=44.811320754717, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI19920794, Length=420, Percent_Identity=44.7619047619048, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45550452, Length=439, Percent_Identity=42.8246013667426, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24653819, Length=403, Percent_Identity=36.7245657568238, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24653817, Length=403, Percent_Identity=33.0024813895782, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48390; Mature: 48259

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLHDRHIKPLILAGLNGDNWHLQDYVERGGYKQLRRILEEKIPPEQVIADVKASGLRG
CCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRQFPGQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYA
CCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHEE
MGITVGYNYIHGEIFEVYRRFEAALEEARTAGFLGDNIMGSGFSFQLHAHHGYGAYICGE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLEMGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCC
NNGGTKIFSVSGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGD
CCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
IMMQTDMDYDSIAKQGSMLGSGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTG
EEEECCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
WLYRVVHRIEHGLGRKEDLDLLNSVAENIMGRTICALGDAAAMPVRGMLKHYWDEFEYHV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
ANKRCLTGGHADAAAASETVAA
CCCEEECCCCCCHHHCHHHHCC
>Mature Secondary Structure 
TSLHDRHIKPLILAGLNGDNWHLQDYVERGGYKQLRRILEEKIPPEQVIADVKASGLRG
CCCCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRQFPGQKYLVCNSDEGEPGTFKDRDILRWNPHALIEGMAIGAYA
CCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHEE
MGITVGYNYIHGEIFEVYRRFEAALEEARTAGFLGDNIMGSGFSFQLHAHHGYGAYICGE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAVGPQNYLEMGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCC
NNGGTKIFSVSGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGD
CCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
IMMQTDMDYDSIAKQGSMLGSGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTG
EEEECCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
WLYRVVHRIEHGLGRKEDLDLLNSVAENIMGRTICALGDAAAMPVRGMLKHYWDEFEYHV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
ANKRCLTGGHADAAAASETVAA
CCCEEECCCCCCHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]