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The map label for this gene is nuoM [H]

Identifier: 186476752

GI number: 186476752

Start: 2246807

End: 2248300

Strand: Reverse

Name: nuoM [H]

Synonym: Bphy_1997

Alternate gene names: 186476752

Gene position: 2248300-2246807 (Counterclockwise)

Preceding gene: 186476753

Following gene: 186476751

Centisome position: 64.62

GC content: 59.57

Gene sequence:

>1494_bases
ATGCACACGACGTTTCCGATTCTGAGTATCGCGATCTGGATGCCGATTATTTTCGGCCTGGTGGTCCTGGCCATCGGTTC
AGATCGCAATCCCGGTCCGGCGCGATGGATTGCGCTGATCGGGTCGGTGTTGAGTTTCATCGTGACGATTCCGCTGATCA
CGGATTTCGACACGAGTACGGCGGCATTGCAGTTCGAAGAAAAGGCTAACTGGATCGAGCGCTTCAACATCACGTACCAC
CTCGGCATCGACGGTATCGCGATGTGGTTCGTCGTGCTGACGGCGCTGATCACGGTGATCGTCGTGATCGCCGCATGGGA
AGTGATCACGAAGAACGTCGCGCAATACCTGGCGGCGTTCCTCATCCTGTCGGGCATCATGGTCGGCGTGTTCTGCGCAG
CTGACGGCATGCTGTTCTACGTGTTCTTCGAAGCGACGCTGATTCCGATGTACATCATCATCGGCGTGTGGGGCGGGGCG
AACCGCGTGTACGCGGCGTTCAAGTTCTTCCTCTATACGCTGATGGGTTCGCTGCTGATGCTGGTGGGCTTGCTGTATCT
GTACACGCAGACGGGCACGTTCGACCTGGCCGCATGGCACGCCGCAAAGTTGTCGATGACGCCGCAGGTGCTGCTCTTCA
TCGCGTTCTTCCTCGCGTTCGCCGTCAAGGTGCCGATGTGGCCTGTCCACACGTGGCTGCCGGACGCGCACGTGGAAGCG
CCGACGGGCGGCTCGGTCGTGCTGGCGGCCATCATGCTGAAGCTCGGTGCGTACGGTTTCCTGCGTTTCTCGCTGCCTAT
CGCACCGGACGCAAGTCACTTCCTCGCACCCGTCGTCATTACGCTGTCGCTGATCGCGGTGGTCTACATCGGCCTCGTCG
CGATGGTGCAGGCCGACATGAAGAAGCTGGTTGCGTATTCGTCGATTGCGCACATGGGCTTCGTCACGCTCGGCTTCTTT
ATCTTCAACCAGCTGGGCACGGAAGGCGCGATCGTGCAGATGATCTCGCACGGTTTCGTGTCGGGCGCGATGTTCCTTTG
CATCGGCGTGCTGTATGACCGCATGCACTCGCGTCAGATCGCCGACTACGGCGGCGTCGTGAACACGATGCCGAAGTTCG
CGGCGCTCGTGATGCTGTTCTCGATGGCGAACTGCGGCCTACCGGGCACCTCCGGTTTCGTCGGCGAGTTCATGGTGATT
CTGGCCGCTGTCCAGTACAACTTCTGGATCGCGTTCGGCGCGGCCGTGACGCTGATTCTCGGCGCGGCCTATACGTTGTG
GATGTACAAGCGCGTGTACTTCGGCGCGGTTGTGAACGATCACGTGAAAAGCCTCATCGACATCAACCGCCGCGAATTCT
TCATGCTGGGGGTGCTCGCGGTGTTGACGCTGTACATGGGCATCTATCCGAAGCCCTTTACCGAAGTAATGCACGTGTCG
GTGGAAAATCTCCTGTCCCACGTTGCGCAGTCGAAGCTGCCGCTACCGCAGTAA

Upstream 100 bases:

>100_bases
ATCTACCACTACGCATTCGCCATGATTATCGGCATGCTGGGGCTCTTGACCCTGTTTGTAACGCTCGGCGGCAAATAAGA
ACAGGCTGGAGACACTTCTC

Downstream 100 bases:

>100_bases
TGCGAAGCGGAGGAATTTAAAGATCATGCAAAACGCACCTATGACCGCCTTGTTACCCGACGCGCTGGTGATGGCCGCTA
TCGTCGTCGCGTGGCTCATC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MHTTFPILSIAIWMPIIFGLVVLAIGSDRNPGPARWIALIGSVLSFIVTIPLITDFDTSTAALQFEEKANWIERFNITYH
LGIDGIAMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFLILSGIMVGVFCAADGMLFYVFFEATLIPMYIIIGVWGGA
NRVYAAFKFFLYTLMGSLLMLVGLLYLYTQTGTFDLAAWHAAKLSMTPQVLLFIAFFLAFAVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAIMLKLGAYGFLRFSLPIAPDASHFLAPVVITLSLIAVVYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFF
IFNQLGTEGAIVQMISHGFVSGAMFLCIGVLYDRMHSRQIADYGGVVNTMPKFAALVMLFSMANCGLPGTSGFVGEFMVI
LAAVQYNFWIAFGAAVTLILGAAYTLWMYKRVYFGAVVNDHVKSLIDINRREFFMLGVLAVLTLYMGIYPKPFTEVMHVS
VENLLSHVAQSKLPLPQ

Sequences:

>Translated_497_residues
MHTTFPILSIAIWMPIIFGLVVLAIGSDRNPGPARWIALIGSVLSFIVTIPLITDFDTSTAALQFEEKANWIERFNITYH
LGIDGIAMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFLILSGIMVGVFCAADGMLFYVFFEATLIPMYIIIGVWGGA
NRVYAAFKFFLYTLMGSLLMLVGLLYLYTQTGTFDLAAWHAAKLSMTPQVLLFIAFFLAFAVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAIMLKLGAYGFLRFSLPIAPDASHFLAPVVITLSLIAVVYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFF
IFNQLGTEGAIVQMISHGFVSGAMFLCIGVLYDRMHSRQIADYGGVVNTMPKFAALVMLFSMANCGLPGTSGFVGEFMVI
LAAVQYNFWIAFGAAVTLILGAAYTLWMYKRVYFGAVVNDHVKSLIDINRREFFMLGVLAVLTLYMGIYPKPFTEVMHVS
VENLLSHVAQSKLPLPQ
>Mature_497_residues
MHTTFPILSIAIWMPIIFGLVVLAIGSDRNPGPARWIALIGSVLSFIVTIPLITDFDTSTAALQFEEKANWIERFNITYH
LGIDGIAMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFLILSGIMVGVFCAADGMLFYVFFEATLIPMYIIIGVWGGA
NRVYAAFKFFLYTLMGSLLMLVGLLYLYTQTGTFDLAAWHAAKLSMTPQVLLFIAFFLAFAVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAIMLKLGAYGFLRFSLPIAPDASHFLAPVVITLSLIAVVYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFF
IFNQLGTEGAIVQMISHGFVSGAMFLCIGVLYDRMHSRQIADYGGVVNTMPKFAALVMLFSMANCGLPGTSGFVGEFMVI
LAAVQYNFWIAFGAAVTLILGAAYTLWMYKRVYFGAVVNDHVKSLIDINRREFFMLGVLAVLTLYMGIYPKPFTEVMHVS
VENLLSHVAQSKLPLPQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=356, Percent_Identity=34.5505617977528, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI251831117, Length=409, Percent_Identity=23.960880195599, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1788613, Length=468, Percent_Identity=39.5299145299145, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788827, Length=284, Percent_Identity=25, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788829, Length=330, Percent_Identity=30.3030303030303, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788831, Length=275, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI145693160, Length=427, Percent_Identity=24.1217798594848, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=379, Percent_Identity=25.3298153034301, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54586; Mature: 54586

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]