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The map label for this gene is hsrA [H]

Identifier: 186476562

GI number: 186476562

Start: 2062272

End: 2063660

Strand: Reverse

Name: hsrA [H]

Synonym: Bphy_1804

Alternate gene names: 186476562

Gene position: 2063660-2062272 (Counterclockwise)

Preceding gene: 186476563

Following gene: 186476560

Centisome position: 59.31

GC content: 67.17

Gene sequence:

>1389_bases
ATGACCGACCGCGCCCGCATCATTCCGCTGATCGTTGCCTGTCCTTTGTTCCTGCAAAACCTCGATACGTCGATCATGGC
GACCGCGCTGCCTTCCATCGCGCGCTCGCTCGGCGTCGAGGCGCTGCATCTGAATCTCGCGATCACTTCCTATCTGCTGA
GCCTTGCCGTGTGCCTGCCGGCGAGCGGCTGGCTTGCCGACCGCTTTGGCGCGCGCCGCGTGTTCTGCTCGGCGATCGGC
CTGTTTTCTCTGGCGTCGGCGTTATGCGGAATCGCCATGTCCCTGCCGCAGCTCGTCGTGTTTCGCGTGCTGCAGGGCAT
GGGCGGCGCGATGATGGTGCCCGTCGGCCGGTTGATCCTGCTGCGCAGCGTGCCGCCCGCGCGCATGGTGGCGGCGATGG
TGTGGTTCACGGTGCCGGGCGCGGTGGGGCGGCTCGCGGGACCGCTGCTCGGCGGCGTGGTGGTGACGTTCACGTCGTGG
CGCTGGATCTTCCTGTTGAACGTGCCGTTTGGCGTGATCGGCGTGCTGTGCGCGCTCGCGTTCATCGAAGACACGCATGA
CGCGCTCGACGAGCGCTTCGATCTGCCCGGGTTCATCCTCCTGTCGACGGGGCTCGTCGGTCTGGTCGGCGGGCTGGATA
CGGCGGGCCGCGGGCTCGTGCCGCAATGGGTCACCTTCGCGTTCACGGGCACGGGTGCGCTGTCGATGCTGCTGTATTGG
CTGTATAGCCGCCGTCACGCCGATGCGATCATCGATCCCTTGCTGCTGCGCTACAAGGCATACCGGACGGCTGTGCTCGG
CGGGATGCCGCTGCGCATCGGCATCGGCGCGTCGCCGTTCCTGATGCCGTTGATGCTGCAGCTCGGGTTCGGCCTGTCGC
CGCTCACGTCGGGATCGCTGAGCGTGGCGACGGCGGTCGGCTCGCTCGCCGTGCGCGGCGTGATGAGCACCGCGATCCGG
CGCATCGGCTTTCGCACGCTGCTGATCGCTGCGACGTGCGTGACGGGTTGCTTCTACGCGTGCTATGGGCTCTTCAGGCC
CGGCACGCCGCATCTGCTGATCTTTTGCGTGCTGCTGGTGGGCGGCCTGTTCAATTCGCTCGCGATGGTCACGCTCAACA
CGCTTGGCTATGCCGACATGCCCAAGCCGAAGATGAGCCGTGCGACGGCGCTGTCCGGCATGGCGCAGCAACTGTCGGTG
AGTCTCGGCGTGGCGTTCGGCGCGTCGCTGCTCGTTCTGACGGCCCGTCTGCACGGCGGCAGCGCCGCACATCTCGCCGC
GCGCGATTTCTCACCGGCGTTCTTCGTGGTGGGCGCGGCCGTGCTGTGCTCGCTGTTCTTCTTCGTGAAGCTGTCGAAGG
AGGAGGGCGCGCAGTTGCGGCAGCTCTGA

Upstream 100 bases:

>100_bases
GCGATCCATCGGTGCGATTCATGGCGTGCGCAACAAACGGTAATGCCGTCGTCCACAGTGACGCTCGCCACATGCCGCCG
TTTTGCGGTAAAAGAAAAGG

Downstream 100 bases:

>100_bases
TCGGGCGGCGCGGCGCGCTTGCAACGTGCTAGCCGCGCCTGCGCCGCGCGACGAACTCGCCTTCCAGATTCCACGTGACG
CCGTCCGGCGCGAGCGCGAC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTDRARIIPLIVACPLFLQNLDTSIMATALPSIARSLGVEALHLNLAITSYLLSLAVCLPASGWLADRFGARRVFCSAIG
LFSLASALCGIAMSLPQLVVFRVLQGMGGAMMVPVGRLILLRSVPPARMVAAMVWFTVPGAVGRLAGPLLGGVVVTFTSW
RWIFLLNVPFGVIGVLCALAFIEDTHDALDERFDLPGFILLSTGLVGLVGGLDTAGRGLVPQWVTFAFTGTGALSMLLYW
LYSRRHADAIIDPLLLRYKAYRTAVLGGMPLRIGIGASPFLMPLMLQLGFGLSPLTSGSLSVATAVGSLAVRGVMSTAIR
RIGFRTLLIAATCVTGCFYACYGLFRPGTPHLLIFCVLLVGGLFNSLAMVTLNTLGYADMPKPKMSRATALSGMAQQLSV
SLGVAFGASLLVLTARLHGGSAAHLAARDFSPAFFVVGAAVLCSLFFFVKLSKEEGAQLRQL

Sequences:

>Translated_462_residues
MTDRARIIPLIVACPLFLQNLDTSIMATALPSIARSLGVEALHLNLAITSYLLSLAVCLPASGWLADRFGARRVFCSAIG
LFSLASALCGIAMSLPQLVVFRVLQGMGGAMMVPVGRLILLRSVPPARMVAAMVWFTVPGAVGRLAGPLLGGVVVTFTSW
RWIFLLNVPFGVIGVLCALAFIEDTHDALDERFDLPGFILLSTGLVGLVGGLDTAGRGLVPQWVTFAFTGTGALSMLLYW
LYSRRHADAIIDPLLLRYKAYRTAVLGGMPLRIGIGASPFLMPLMLQLGFGLSPLTSGSLSVATAVGSLAVRGVMSTAIR
RIGFRTLLIAATCVTGCFYACYGLFRPGTPHLLIFCVLLVGGLFNSLAMVTLNTLGYADMPKPKMSRATALSGMAQQLSV
SLGVAFGASLLVLTARLHGGSAAHLAARDFSPAFFVVGAAVLCSLFFFVKLSKEEGAQLRQL
>Mature_461_residues
TDRARIIPLIVACPLFLQNLDTSIMATALPSIARSLGVEALHLNLAITSYLLSLAVCLPASGWLADRFGARRVFCSAIGL
FSLASALCGIAMSLPQLVVFRVLQGMGGAMMVPVGRLILLRSVPPARMVAAMVWFTVPGAVGRLAGPLLGGVVVTFTSWR
WIFLLNVPFGVIGVLCALAFIEDTHDALDERFDLPGFILLSTGLVGLVGGLDTAGRGLVPQWVTFAFTGTGALSMLLYWL
YSRRHADAIIDPLLLRYKAYRTAVLGGMPLRIGIGASPFLMPLMLQLGFGLSPLTSGSLSVATAVGSLAVRGVMSTAIRR
IGFRTLLIAATCVTGCFYACYGLFRPGTPHLLIFCVLLVGGLFNSLAMVTLNTLGYADMPKPKMSRATALSGMAQQLSVS
LGVAFGASLLVLTARLHGGSAAHLAARDFSPAFFVVGAAVLCSLFFFVKLSKEEGAQLRQL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=463, Percent_Identity=37.5809935205184, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788392, Length=421, Percent_Identity=40.3800475059382, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1788710, Length=424, Percent_Identity=26.1792452830189, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789042, Length=441, Percent_Identity=25.6235827664399, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87081983, Length=202, Percent_Identity=34.1584158415842, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48888; Mature: 48757

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRARIIPLIVACPLFLQNLDTSIMATALPSIARSLGVEALHLNLAITSYLLSLAVCLP
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
ASGWLADRFGARRVFCSAIGLFSLASALCGIAMSLPQLVVFRVLQGMGGAMMVPVGRLIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
LRSVPPARMVAAMVWFTVPGAVGRLAGPLLGGVVVTFTSWRWIFLLNVPFGVIGVLCALA
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHH
FIEDTHDALDERFDLPGFILLSTGLVGLVGGLDTAGRGLVPQWVTFAFTGTGALSMLLYW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCHHHHHHHHHH
LYSRRHADAIIDPLLLRYKAYRTAVLGGMPLRIGIGASPFLMPLMLQLGFGLSPLTSGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCH
SVATAVGSLAVRGVMSTAIRRIGFRTLLIAATCVTGCFYACYGLFRPGTPHLLIFCVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GGLFNSLAMVTLNTLGYADMPKPKMSRATALSGMAQQLSVSLGVAFGASLLVLTARLHGG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SAAHLAARDFSPAFFVVGAAVLCSLFFFVKLSKEEGAQLRQL
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCHHHHCC
>Mature Secondary Structure 
TDRARIIPLIVACPLFLQNLDTSIMATALPSIARSLGVEALHLNLAITSYLLSLAVCLP
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
ASGWLADRFGARRVFCSAIGLFSLASALCGIAMSLPQLVVFRVLQGMGGAMMVPVGRLIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
LRSVPPARMVAAMVWFTVPGAVGRLAGPLLGGVVVTFTSWRWIFLLNVPFGVIGVLCALA
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHH
FIEDTHDALDERFDLPGFILLSTGLVGLVGGLDTAGRGLVPQWVTFAFTGTGALSMLLYW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCHHHHHHHHHH
LYSRRHADAIIDPLLLRYKAYRTAVLGGMPLRIGIGASPFLMPLMLQLGFGLSPLTSGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCH
SVATAVGSLAVRGVMSTAIRRIGFRTLLIAATCVTGCFYACYGLFRPGTPHLLIFCVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GGLFNSLAMVTLNTLGYADMPKPKMSRATALSGMAQQLSVSLGVAFGASLLVLTARLHGG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SAAHLAARDFSPAFFVVGAAVLCSLFFFVKLSKEEGAQLRQL
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]