Click here to switch to the map view.

The map label for this gene is yneJ [H]

Identifier: 186476370

GI number: 186476370

Start: 1830799

End: 1831704

Strand: Direct

Name: yneJ [H]

Synonym: Bphy_1612

Alternate gene names: 186476370

Gene position: 1830799-1831704 (Clockwise)

Preceding gene: 186476361

Following gene: 186476372

Centisome position: 52.62

GC content: 66.45

Gene sequence:

>906_bases
ATGGATCTTGCCGCCCTCACCATCTTTCGCGCGGTCGTGCGCGAAAACGGCGTGACGCGCGCCGCCGCGAAGCTCAATCG
CGTGCAGTCGAATGTGACGACGCGTATCAGGCAACTCGAAGAGCAACTGGGCACCGAACTCTTCACCCGCGACGGCCGGC
GGCTCGTGCTGACGCCCGCAGGCGAAACGTTGCTGCCCTACGCCGAACGGCTGCTCGCGCTCGCGGACGAGGCGCGGCAT
GCCGTCAAGGACGCGCAGCCAAGCGGCCGGCTGCGCCTCGGCACGATGGAAAGTGTCGCCGCGACCCGGCTGCCCGGACT
GCTCGCGCACTATCACCAGACCTGGCCTGAAGTCGCGCTCGAACTGGAGGTGGGGACCACGGACAAGCTGATCGACCGCG
TGCGCGAATTCGAAGTGGATGCCGCGATCGTTGCGAGGCCGCTCGACGCCGGCTGGATCGGCGATCTGTTCGAGGTCGAG
GCCGTGTTCCGCGAAGAACTGGTGATGCTGACGCCGCGCGGTCATCCGCCTGTCCGCGAGCCGAGCGACATTACCCTGTC
GACGCTGATCGCGTTCGAAACGGGCTGCGCGTATCGCGTGCATGTTGAGAAGTGGTATATGGATCGAGGCCTGCGGCCGG
CACGCGTGCTGGAACTCGGCTCGTATCACGCGATCGTCGCGTGCGTGGCGGCGGGCGCGGGTGTGGCTGTCGCCCCACGT
TCGGTGCTCGCGCTGCTGCCCGAATCGAACGACATCGCCGTGCATGCGCTCGACGATCTGGGCAGCATCGATACGCTGCT
GATCTGGCGGCGCGGGCATTTTTCGTCAGCGTTGAATGCGTTGCGCGGGATGCTGGTTGCGAGCAGCAACCTGCGGGACA
AGGCGGCGGAAGAAGCAGCCGTCTGA

Upstream 100 bases:

>100_bases
AATAGAGGAGCGACAGAGTGCTTTCACGCTGCATCCTAGCTTGACCCATATATCTCGTAAAATGAATAATCTTGATGTAT
CGAATCTCATGGAGAGATTG

Downstream 100 bases:

>100_bases
GCGGCCCGGGAGGGACGCCGTCAACTGATCGATATAAACAAAAAAAGCCGCAAGTCCTATCGACTCGCGGCCTTCTTTTA
TGCATAAGCGGATGCTACGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDLAALTIFRAVVRENGVTRAAAKLNRVQSNVTTRIRQLEEQLGTELFTRDGRRLVLTPAGETLLPYAERLLALADEARH
AVKDAQPSGRLRLGTMESVAATRLPGLLAHYHQTWPEVALELEVGTTDKLIDRVREFEVDAAIVARPLDAGWIGDLFEVE
AVFREELVMLTPRGHPPVREPSDITLSTLIAFETGCAYRVHVEKWYMDRGLRPARVLELGSYHAIVACVAAGAGVAVAPR
SVLALLPESNDIAVHALDDLGSIDTLLIWRRGHFSSALNALRGMLVASSNLRDKAAEEAAV

Sequences:

>Translated_301_residues
MDLAALTIFRAVVRENGVTRAAAKLNRVQSNVTTRIRQLEEQLGTELFTRDGRRLVLTPAGETLLPYAERLLALADEARH
AVKDAQPSGRLRLGTMESVAATRLPGLLAHYHQTWPEVALELEVGTTDKLIDRVREFEVDAAIVARPLDAGWIGDLFEVE
AVFREELVMLTPRGHPPVREPSDITLSTLIAFETGCAYRVHVEKWYMDRGLRPARVLELGSYHAIVACVAAGAGVAVAPR
SVLALLPESNDIAVHALDDLGSIDTLLIWRRGHFSSALNALRGMLVASSNLRDKAAEEAAV
>Mature_301_residues
MDLAALTIFRAVVRENGVTRAAAKLNRVQSNVTTRIRQLEEQLGTELFTRDGRRLVLTPAGETLLPYAERLLALADEARH
AVKDAQPSGRLRLGTMESVAATRLPGLLAHYHQTWPEVALELEVGTTDKLIDRVREFEVDAAIVARPLDAGWIGDLFEVE
AVFREELVMLTPRGHPPVREPSDITLSTLIAFETGCAYRVHVEKWYMDRGLRPARVLELGSYHAIVACVAAGAGVAVAPR
SVLALLPESNDIAVHALDDLGSIDTLLIWRRGHFSSALNALRGMLVASSNLRDKAAEEAAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=286, Percent_Identity=40.2097902097902, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082132, Length=252, Percent_Identity=30.952380952381, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=28.8659793814433, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788887, Length=287, Percent_Identity=27.8745644599303, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1790208, Length=258, Percent_Identity=30.2325581395349, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI157672245, Length=232, Percent_Identity=28.8793103448276, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788296, Length=253, Percent_Identity=29.6442687747036, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=27.5862068965517, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693105, Length=259, Percent_Identity=23.1660231660232, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787601, Length=175, Percent_Identity=26.8571428571429, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788626, Length=148, Percent_Identity=35.1351351351351, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994962, Length=147, Percent_Identity=34.6938775510204, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787589, Length=136, Percent_Identity=36.7647058823529, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787530, Length=282, Percent_Identity=24.113475177305, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=24.1935483870968, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=192, Percent_Identity=28.125, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081904, Length=224, Percent_Identity=26.3392857142857, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790399, Length=264, Percent_Identity=24.2424242424242, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367136, Length=119, Percent_Identity=36.9747899159664, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32948; Mature: 32948

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALTIFRAVVRENGVTRAAAKLNRVQSNVTTRIRQLEEQLGTELFTRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEECC
GETLLPYAERLLALADEARHAVKDAQPSGRLRLGTMESVAATRLPGLLAHYHQTWPEVAL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCHHEEE
ELEVGTTDKLIDRVREFEVDAAIVARPLDAGWIGDLFEVEAVFREELVMLTPRGHPPVRE
EEECCCHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCC
PSDITLSTLIAFETGCAYRVHVEKWYMDRGLRPARVLELGSYHAIVACVAAGAGVAVAPR
CCCCHHHHHHHHHCCCEEEEEHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLALLPESNDIAVHALDDLGSIDTLLIWRRGHFSSALNALRGMLVASSNLRDKAAEEAA
HHHEECCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCC
V
C
>Mature Secondary Structure
MDLAALTIFRAVVRENGVTRAAAKLNRVQSNVTTRIRQLEEQLGTELFTRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEECC
GETLLPYAERLLALADEARHAVKDAQPSGRLRLGTMESVAATRLPGLLAHYHQTWPEVAL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCHHEEE
ELEVGTTDKLIDRVREFEVDAAIVARPLDAGWIGDLFEVEAVFREELVMLTPRGHPPVRE
EEECCCHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCC
PSDITLSTLIAFETGCAYRVHVEKWYMDRGLRPARVLELGSYHAIVACVAAGAGVAVAPR
CCCCHHHHHHHHHCCCEEEEEHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLALLPESNDIAVHALDDLGSIDTLLIWRRGHFSSALNALRGMLVASSNLRDKAAEEAA
HHHEECCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]