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The map label for this gene is yeiT [H]

Identifier: 186476293

GI number: 186476293

Start: 1733617

End: 1734966

Strand: Reverse

Name: yeiT [H]

Synonym: Bphy_1535

Alternate gene names: 186476293

Gene position: 1734966-1733617 (Counterclockwise)

Preceding gene: 186476294

Following gene: 186476292

Centisome position: 49.87

GC content: 66.52

Gene sequence:

>1350_bases
ATGGCCATCAAGCAAACAGGCGACATCGCCGCGCATCGTCTGTCGCCCGACCAGCTTTCGTGTGAATTCGCCGACATTGC
GCCGCTGCTCGATTCCACCGCAGCGGCCGCTGCCGCGAGCCGCTGTCACTACTGCTATGACGCGCCTTGCGTGCAGGCCT
GTCCCACGCAAATCGACATCCCCAGCTTCATCCGCAAGATTGGCAACGGCAATCTGAAGGGCGCCGCAACCGACATCCTG
TCCGCGAACCCATTGGGCGGCATGTGTTCGCGTGTCTGCCCGACGGAGATTCTTTGCGAAGGCGCATGCGTGCGCAATCA
TCAGGACGCACAGCCCGTCGCGATCGGCGCGTTGCAGCGTCATGCGACGGATTGGGCGATGGCGCACGATGCCGTGCAGT
GGCAGCGTGCGCCCGACAGCGGACGTCATGTCGCCGTCGTCGGCGCGGGACCGGCGGGGCTCGCCTGTGCGCATCGCCTG
GCGGTGGCGGGGCATCGCGTGACGATTTACGACGCCCGCGACAAGGGCGGCGGACTCAACGAATATGGCATCGCCGCGTA
CAAGACGGTCGACGGCTTCGCGCAGCGCGAAGTGCAATGGCTGCTGGCGGTGGGCGGGATCGACCTGAAAACAGGTGTTG
CGCTCGGCCGTGACGTGACGCTCGATGCGCTGCGCCAGCAGCACGATGCCGTGTTTCTCGCGCTCGGTCTGACGGGCGTG
CGCGCGCTCGAACTCGATGGGGAAGCGTTGGGCGGCGTGCTTGACGCCGTCGATTTCATCGAGCAGGTGCGTGAGGCGTC
AGACCTCGCGACGGTTGCCGTTGGCCGCCGCGTGGTAGTGATCGGCGGCGGCAATACGGCTATCGATGCCGCGGTGCAAA
GCCGCAAACTCGGCGCGACGAGCGTGACGATGGTGTACCGGCGCGGTGTCGAATCGATGAGCGCGACCTGGACCGAGCGC
GAATTCGCGCAGACGCAGGGCGTGACGCTCGTCACGCATGCGAAGCCCGTGCGATTGATAGGCACCGACGGCATGGTGAG
CGCAGTCGAATTCGAACGCACTGCTCGCGATGGCGCCACGGAGCGCTTCACGCTCGAGGCCGACATGGTGCTCAAGGCCA
TCGGCCAGACGCTGGTCGCCGTCGGCCTCGATCGCGCGCTGCTGACGTTCGATCAACACCGCATCGCAGTCGACGCGGAC
ATGCGCACGTCACTGGACAACGTGTGGGCGGGCGGCGATTGCGCGGCGTCGGGCGGCATCGACCTGACCGTGCAAGCGGT
GCAGGACGGCAAGCTCGCAGCGGCATCGATCGACGCGGCGTTCGCATTGGCCGCAGTGAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
TCGAGGCTTCAACGCTGCACCCCTGCAATACGGCGCCCCGGCTTCGTTCGAGCGAAGCCGGCAGGCACGGCTACGTCCTC
ACTTCGTCGAAGGAAAGTAT

Downstream 100 bases:

>100_bases
ACGCGCCGCCCGATATCCAAGGATAAAACCGAACCCCACGGAGCCGAACATGGCCGATCTACGCTGCACGATAGCCGGCA
TCACGTCGCCGAATCCTTTC

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAIKQTGDIAAHRLSPDQLSCEFADIAPLLDSTAAAAAASRCHYCYDAPCVQACPTQIDIPSFIRKIGNGNLKGAATDIL
SANPLGGMCSRVCPTEILCEGACVRNHQDAQPVAIGALQRHATDWAMAHDAVQWQRAPDSGRHVAVVGAGPAGLACAHRL
AVAGHRVTIYDARDKGGGLNEYGIAAYKTVDGFAQREVQWLLAVGGIDLKTGVALGRDVTLDALRQQHDAVFLALGLTGV
RALELDGEALGGVLDAVDFIEQVREASDLATVAVGRRVVVIGGGNTAIDAAVQSRKLGATSVTMVYRRGVESMSATWTER
EFAQTQGVTLVTHAKPVRLIGTDGMVSAVEFERTARDGATERFTLEADMVLKAIGQTLVAVGLDRALLTFDQHRIAVDAD
MRTSLDNVWAGGDCAASGGIDLTVQAVQDGKLAAASIDAAFALAAVKAA

Sequences:

>Translated_449_residues
MAIKQTGDIAAHRLSPDQLSCEFADIAPLLDSTAAAAAASRCHYCYDAPCVQACPTQIDIPSFIRKIGNGNLKGAATDIL
SANPLGGMCSRVCPTEILCEGACVRNHQDAQPVAIGALQRHATDWAMAHDAVQWQRAPDSGRHVAVVGAGPAGLACAHRL
AVAGHRVTIYDARDKGGGLNEYGIAAYKTVDGFAQREVQWLLAVGGIDLKTGVALGRDVTLDALRQQHDAVFLALGLTGV
RALELDGEALGGVLDAVDFIEQVREASDLATVAVGRRVVVIGGGNTAIDAAVQSRKLGATSVTMVYRRGVESMSATWTER
EFAQTQGVTLVTHAKPVRLIGTDGMVSAVEFERTARDGATERFTLEADMVLKAIGQTLVAVGLDRALLTFDQHRIAVDAD
MRTSLDNVWAGGDCAASGGIDLTVQAVQDGKLAAASIDAAFALAAVKAA
>Mature_448_residues
AIKQTGDIAAHRLSPDQLSCEFADIAPLLDSTAAAAAASRCHYCYDAPCVQACPTQIDIPSFIRKIGNGNLKGAATDILS
ANPLGGMCSRVCPTEILCEGACVRNHQDAQPVAIGALQRHATDWAMAHDAVQWQRAPDSGRHVAVVGAGPAGLACAHRLA
VAGHRVTIYDARDKGGGLNEYGIAAYKTVDGFAQREVQWLLAVGGIDLKTGVALGRDVTLDALRQQHDAVFLALGLTGVR
ALELDGEALGGVLDAVDFIEQVREASDLATVAVGRRVVVIGGGNTAIDAAVQSRKLGATSVTMVYRRGVESMSATWTERE
FAQTQGVTLVTHAKPVRLIGTDGMVSAVEFERTARDGATERFTLEADMVLKAIGQTLVAVGLDRALLTFDQHRIAVDADM
RTSLDNVWAGGDCAASGGIDLTVQAVQDGKLAAASIDAAFALAAVKAA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=459, Percent_Identity=31.5904139433551, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI237757300, Length=120, Percent_Identity=44.1666666666667, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI87082180, Length=466, Percent_Identity=33.4763948497854, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1788468, Length=414, Percent_Identity=33.8164251207729, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=32.5678496868476, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789606, Length=449, Percent_Identity=33.184855233853, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789243, Length=415, Percent_Identity=29.6385542168675, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71984108, Length=452, Percent_Identity=32.7433628318584, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17570289, Length=458, Percent_Identity=26.2008733624454, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17543860, Length=167, Percent_Identity=32.3353293413174, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=448, Percent_Identity=30.5803571428571, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640763, Length=449, Percent_Identity=35.4120267260579, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI18858217, Length=449, Percent_Identity=35.4120267260579, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=29.5964125560538, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=29.5964125560538, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24665547, Length=446, Percent_Identity=29.5964125560538, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24665543, Length=446, Percent_Identity=29.5964125560538, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 46932; Mature: 46801

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKQTGDIAAHRLSPDQLSCEFADIAPLLDSTAAAAAASRCHYCYDAPCVQACPTQIDI
CCCCCCCCCHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCH
PSFIRKIGNGNLKGAATDILSANPLGGMCSRVCPTEILCEGACVRNHQDAQPVAIGALQR
HHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHCCCHHHHHCCCHHCCCCCCCCHHHHHHHH
HATDWAMAHDAVQWQRAPDSGRHVAVVGAGPAGLACAHRLAVAGHRVTIYDARDKGGGLN
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
EYGIAAYKTVDGFAQREVQWLLAVGGIDLKTGVALGRDVTLDALRQQHDAVFLALGLTGV
CCCEEEEEHHHHHHHHHHEEEEEECCCEECCCCCCCCCCCHHHHHHHCCEEEEEECCCCE
RALELDGEALGGVLDAVDFIEQVREASDLATVAVGRRVVVIGGGNTAIDAAVQSRKLGAT
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHCCCCCH
SVTMVYRRGVESMSATWTEREFAQTQGVTLVTHAKPVRLIGTDGMVSAVEFERTARDGAT
HHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEHHHHHHHHCCCCC
ERFTLEADMVLKAIGQTLVAVGLDRALLTFDQHRIAVDADMRTSLDNVWAGGDCAASGGI
EEEEEHHHHHHHHHHHHHHHHHCCHHHEEECCCEEEECCHHHHCHHHCCCCCCCCCCCCC
DLTVQAVQDGKLAAASIDAAFALAAVKAA
EEEEEEECCCCEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIKQTGDIAAHRLSPDQLSCEFADIAPLLDSTAAAAAASRCHYCYDAPCVQACPTQIDI
CCCCCCCCHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCH
PSFIRKIGNGNLKGAATDILSANPLGGMCSRVCPTEILCEGACVRNHQDAQPVAIGALQR
HHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHCCCHHHHHCCCHHCCCCCCCCHHHHHHHH
HATDWAMAHDAVQWQRAPDSGRHVAVVGAGPAGLACAHRLAVAGHRVTIYDARDKGGGLN
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
EYGIAAYKTVDGFAQREVQWLLAVGGIDLKTGVALGRDVTLDALRQQHDAVFLALGLTGV
CCCEEEEEHHHHHHHHHHEEEEEECCCEECCCCCCCCCCCHHHHHHHCCEEEEEECCCCE
RALELDGEALGGVLDAVDFIEQVREASDLATVAVGRRVVVIGGGNTAIDAAVQSRKLGAT
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHCCCCCH
SVTMVYRRGVESMSATWTEREFAQTQGVTLVTHAKPVRLIGTDGMVSAVEFERTARDGAT
HHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEHHHHHHHHCCCCC
ERFTLEADMVLKAIGQTLVAVGLDRALLTFDQHRIAVDADMRTSLDNVWAGGDCAASGGI
EEEEEHHHHHHHHHHHHHHHHHCCHHHEEECCCEEEECCHHHHCHHHCCCCCCCCCCCCC
DLTVQAVQDGKLAAASIDAAFALAAVKAA
EEEEEEECCCCEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]